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During tumorigenesis, cancer cells undergo metabolic reprogramming to meet energetic and biosynthetic demands, increasing glycolytic metabolism and reducing oxidative metabolism\u2014a phenomenon known as the Warburg effect. This study aimed to evaluate the impact of butyrate on the aggressiveness-related metabolic phenotype of three colon cancer cell lines (LS1034, C2BBe1, and WiDr). Butyrate\u2019s effects were assessed through fluorine-18 fluorodeoxyglucose ([18F]FDG) uptake, flow cytometry analysis of cytoplasmic and membrane expression of glucose transporters (GLUT1, GLUT3, GLUT5, and GLUT12), lactate production, and analysis of Krebs cycle turnover and glycolysis\u2013Krebs cycle coupling using nuclear magnetic resonance isotopomer profiling. [18F]FDG uptake decreased in C2BBe1 and WiDr cells, whereas an opposite response was observed in LS1034 cells, which also exhibited reduced GLUT5 expression. These uptake patterns were consistent with lactate production measurements, and an enhancement of oxidative metabolism was detected in C2BBe1 and WiDr cells. Although butyrate was consumed by all three cell lines, its metabolic handling appeared to differ in LS1034 cells, possibly reflecting cytotoxic stress and\/or distinct metabolic regulation mechanisms. Overall, these findings indicate that butyrate exerts cell-line-dependent metabolic effects in colorectal cancer cells. In C2BBe1 and WiDr cells, butyrate exposure was broadly consistent with the attenuation of glycolytic\/Warburg-associated features, whereas LS1034 cells displayed a divergent response and were interpreted separately. These data support further investigation of butyrate as a modulator of colorectal cancer cell metabolism, while highlighting the heterogeneity of metabolic responses across tumor models.<\/jats:p>","DOI":"10.3390\/ijms27083696","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T16:10:44Z","timestamp":1776787844000},"page":"3696","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preventive Effect of Butyrate in Colon Cancer Cell Metabolism"],"prefix":"10.3390","volume":"27","author":[{"given":"Telmo Jos\u00e9","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Univ Coimbra, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Biophysics Institute of Faculty of Medicine, 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, Faculty of Pharmacy, 3000-548 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4185-7871","authenticated-orcid":false,"given":"Ana Margarida","family":"Abrantes","sequence":"additional","affiliation":[{"name":"Univ Coimbra, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Biophysics Institute of Faculty of Medicine, 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, Center for Innovative Biomedicine and Biotechnology (CIBB), 3000-548 Coimbra, Portugal"},{"name":"Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7994-4650","authenticated-orcid":false,"given":"Ana Salom\u00e9","family":"Pires","sequence":"additional","affiliation":[{"name":"Univ Coimbra, Coimbra Institute for Clinical and Biomedical Research (iCBR) area of Environment Genetics and Oncobiology (CIMAGO), Biophysics 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Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2324-1259","authenticated-orcid":false,"given":"Ludgero Can\u00e1rio","family":"Tavares","sequence":"additional","affiliation":[{"name":"Univ Coimbra, Center for Innovative Biomedicine and Biotechnology (CIBB), 3000-548 Coimbra, Portugal"},{"name":"Univ Coimbra, CNC-Center for Neuroscience and Cell Biology, 3004-504 Coimbra, Portugal"},{"name":"Vasco da Gama Research Center (CIVG), University School Vasco da Gama (EUVG), 3020-210 Coimbra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o","family":"Casalta-Lopes","sequence":"additional","affiliation":[{"name":"Coimbra Health School, Polytechnic University of Coimbra, 3045-093 Coimbra, Portugal"},{"name":"Department of Radiotherapy, Unidade Local de Sa\u00fade de S\u00e3o Jo\u00e3o, 4200-319 Porto, Portugal"},{"name":"Life and Health Sciences Research Institute (ICVS), School of Medicine, University 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36 Cancers in 185 Countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1500902","DOI":"10.1002\/mnfr.201500902","article-title":"Interplay between Diet, Gut Microbiota, Epigenetic Events, and Colorectal Cancer","volume":"61","author":"Bultman","year":"2017","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Yang, Y.-C., Chang, S.-C., Hung, C.-S., Shen, M.-H., Lai, C.-L., and Huang, C.-J. (2025). Gut-Microbiota-Derived Metabolites and Probiotic Strategies in Colorectal Cancer: Implications for Disease Modulation and Precision Therapy. Nutrients, 17.","DOI":"10.3390\/nu17152501"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s10555-015-9578-9","article-title":"Revisit Dietary Fiber on Colorectal Cancer: Butyrate and Its Role on Prevention and Treatment","volume":"34","author":"Abrantes","year":"2015","journal-title":"Cancer Metastasis Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/j.1440-1746.2006.04213.x","article-title":"Does Butyrate Protect from Colorectal Cancer?","volume":"21","author":"Sengupta","year":"2006","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1007\/s12029-019-00329-3","article-title":"Importance of the Microbiota Inhibitory Mechanism on the Warburg Effect in Colorectal Cancer Cells","volume":"51","author":"Eslami","year":"2020","journal-title":"J. Gastrointest. Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.pharmthera.2008.09.005","article-title":"Nutrient Transporters in Cancer: Relevance to Warburg Hypothesis and Beyond","volume":"121","author":"Ganapathy","year":"2009","journal-title":"Pharmacol. Ther."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"874904","DOI":"10.1155\/2015\/874904","article-title":"Aerobic Glycolysis as a Marker of Tumor Aggressiveness: Preliminary Data in High Grade Human Brain Tumors","volume":"2015","author":"Vlassenko","year":"2015","journal-title":"Dis. Markers"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.cell.2011.02.013","article-title":"Hallmarks of Cancer: The next Generation","volume":"144","author":"Hanahan","year":"2011","journal-title":"Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1002\/ijc.25599","article-title":"Butyrate Elicits a Metabolic Switch in Human Colon Cancer Cells by Targeting the Pyruvate Dehydrogenase Complex","volume":"128","author":"Blouin","year":"2011","journal-title":"Int. J. Cancer"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.molcel.2012.08.033","article-title":"The Warburg Effect Dictates the Mechanism of Butyrate-Mediated Histone Acetylation and Cell Proliferation","volume":"48","author":"Donohoe","year":"2012","journal-title":"Mol. Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"117900","DOI":"10.1016\/j.biopha.2025.117900","article-title":"Tailor-Made Biochar Enhances the Anti-Tumour Effects of Butyrate-Glycerides in Colorectal Cancer","volume":"184","author":"Fei","year":"2025","journal-title":"Biomed. Pharmacother."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1074\/mcp.RA118.000752","article-title":"Butyrate Suppresses the Proliferation of Colorectal Cancer Cells via Targeting Pyruvate Kinase M2 and Metabolic Reprogramming","volume":"17","author":"Li","year":"2018","journal-title":"Mol. Cell. Proteom."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"759791","DOI":"10.1155\/2014\/759791","article-title":"Metabolic Effects of Hypoxia in Colorectal Cancer by 13C NMR Isotopomer Analysis","volume":"2014","author":"Abrantes","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1038\/nrc2981","article-title":"Regulation of Cancer Cell Metabolism","volume":"11","author":"Cairns","year":"2011","journal-title":"Nat. Rev. Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"22700","DOI":"10.1074\/jbc.M801765200","article-title":"Pyruvate Dehydrogenase Complex Activity Controls Metabolic and Malignant Phenotype in Cancer Cells","volume":"283","author":"McFate","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.semcdb.2012.02.003","article-title":"New Aspects of the Warburg Effect in Cancer Cell Biology","volume":"23","author":"Bensinger","year":"2012","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.molcel.2014.09.026","article-title":"A Role for the Mitochondrial Pyruvate Carrier as a Repressor of the Warburg Effect and Colon Cancer Cell Growth","volume":"56","author":"Schell","year":"2014","journal-title":"Mol. Cell"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.jnutbio.2018.02.018","article-title":"Butyrate, a Dietary Fiber Derivative That Improves Irinotecan Effect in Colon Cancer Cells","volume":"56","author":"Pires","year":"2018","journal-title":"J. Nutr. Biochem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"408","DOI":"10.4236\/jct.2011.23056","article-title":"Efflux Pumps Modulation in Colorectal Adenocarcinoma Cell Lines: The Role of Nuclear Medicine","volume":"02","author":"Abrantes","year":"2011","journal-title":"J. Cancer Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.2967\/jnumed.108.054098","article-title":"18F-FDG Uptake in Lung, Breast, and Colon Cancers: Molecular Biology Correlates and Disease Characterization","volume":"50","author":"Jadvar","year":"2009","journal-title":"J. Nucl. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1590\/S1807-59322011000600008","article-title":"GLUT1 Expression in Malignant Tumors and Its Use as an Immunodiagnostic Marker","volume":"66","author":"Carvalho","year":"2011","journal-title":"Clinics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1042\/bj2980629","article-title":"Presence and Differential Expression of SGLT1, GLUT1, GLUT2, GLUT3 and GLUT5 Hexose-Transporter MRNAs in Caco-2 Cell Clones in Relation to Cell Growth and Glucose Consumption","volume":"298","author":"Mahraoui","year":"1994","journal-title":"Biochem. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s13105-024-01028-9","article-title":"The Glucose Transporter GLUT12, a New Actor in Obesity and Cancer","volume":"81","author":"Burgos","year":"2025","journal-title":"J. Physiol. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1002\/jcp.20166","article-title":"Molecular and Cellular Regulation of Glucose Transporter (GLUT) Proteins in Cancer","volume":"202","author":"Macheda","year":"2005","journal-title":"J. Cell. Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1097\/00006676-199309000-00010","article-title":"Effect of Sodium Butyrate on Glucose Transport and Glucose-Phosphorylating Enzymes in RIN-MSF Cells","volume":"8","author":"Davidson","year":"1993","journal-title":"Pancreas"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1002\/jcp.21885","article-title":"Kinetics of Transport and Phosphorylation of Glucose in Cancer Cells","volume":"221","year":"2009","journal-title":"J. Cell. Physiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1097\/00006676-198902000-00009","article-title":"Effect of Sodium Butyrate, A Differentiating Agent, on Cell Surface Glycoconjugates of a Human Pancreatic Cell Line","volume":"4","author":"Bloom","year":"1989","journal-title":"Pancreas"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4938","DOI":"10.1128\/MCB.06120-11","article-title":"Tyrosine Phosphorylation of Lactate Dehydrogenase A Is Important for NADH\/NAD+ Redox Homeostasis in Cancer Cells","volume":"31","author":"Fan","year":"2011","journal-title":"Mol. Cell. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.ejphar.2012.11.029","article-title":"The Effect of Oxidative Stress upon the Intestinal Epithelial Uptake of Butyrate","volume":"699","author":"Catarino","year":"2013","journal-title":"Eur. J. Pharmacol."},{"key":"ref_31","first-page":"465","article-title":"Evaluation the Effects of Some Cobalt Amino Acids Complexes Using Leukocytes as in vitro Model for Cytotoxicity. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca","volume":"66","author":"Stanila","year":"1970","journal-title":"Agriculture"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1002\/jcp.21556","article-title":"Butyrate Metabolism in Human Colon Carcinoma Cells: Implications Concerning Its Growth-Inhibitory Effect","volume":"218","author":"Andriamihaja","year":"2009","journal-title":"J. Cell. Physiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1007\/s10620-005-2463-6","article-title":"Butyrate-Induced Differentiation of Colon Cancer Cells Is PKC and JNK Dependent","volume":"50","author":"Orchel","year":"2005","journal-title":"Dig. Dis. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.jceh.2015.05.003","article-title":"Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and P53 Expression","volume":"5","author":"Brito","year":"2015","journal-title":"J. Clin. Exp. Hepatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"9637","DOI":"10.1074\/jbc.M116.764597","article-title":"Effects of Altered Sialic Acid Biosynthesis on N-Linked Glycan Branching and Cell Surface Interactions","volume":"292","author":"Pham","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1007\/s00216-010-4425-x","article-title":"Metabolite Extraction from Adherently Growing Mammalian Cells for Metabolomics Studies: Optimization of Harvesting and Extraction Protocols","volume":"399","author":"Dettmer","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1007\/s12012-011-9112-4","article-title":"Metabolic Remodeling during H9c2 Myoblast Differentiation: Relevance for in Vitro Toxicity Studies","volume":"11","author":"Pereira","year":"2011","journal-title":"Cardiovasc. Toxicol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"59","DOI":"10.3390\/i7020059","article-title":"Downregulation of the Expression of GLUT1 Plays a Role in Apoptosis Induced by Sodium Butyrate in HT-29 Cell Line","volume":"7","author":"Li","year":"2006","journal-title":"Int. J. Mol. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Amo\u00eado, N.D., Rodrigues, M.F., Pezzuto, P., Galina, A., da Costa, R.M., de Almeida, F.C.L., El-Bacha, T., and Rumjanek, F.D. (2011). Energy Metabolism in H460 Lung Cancer Cells: Effects of Histone Deacetylase Inhibitors. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0022264"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1002\/stem.1662","article-title":"Targeting Unique Metabolic Properties of Breast Tumor Initiating Cells","volume":"32","author":"Feng","year":"2014","journal-title":"Stem Cells"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1002\/jcb.24671","article-title":"Energy Metabolism Characterization of a Novel Cancer Stem Cell-like Line 3AB-OS","volume":"115","author":"Palorini","year":"2014","journal-title":"J. Cell. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.4155\/fmc.12.80","article-title":"Histone Deacetylase Inhibitors in the Treatment of Cancer: Overview and Perspectives","volume":"4","author":"Giannini","year":"2012","journal-title":"Future Med. Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/8\/3696\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T04:32:28Z","timestamp":1777005148000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/8\/3696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,21]]},"references-count":42,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["ijms27083696"],"URL":"https:\/\/doi.org\/10.3390\/ijms27083696","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,21]]}}}