{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:13:14Z","timestamp":1785413594849,"version":"3.56.0"},"reference-count":50,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"crossref","award":["202208080236"],"award-info":[{"award-number":["202208080236"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"crossref"}]},{"award":["202208080236"],"award-info":[{"award-number":["202208080236"]}],"id":[{"id":"https:\/\/ror.org\/04atp4p48","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003336","name":"Bulgarian National Science Fund","doi-asserted-by":"crossref","award":["KP-06-H81\/9"],"award-info":[{"award-number":["KP-06-H81\/9"]}],"id":[{"id":"10.13039\/501100003336","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ovarian tissue cryopreservation is the primary fertility preservation strategy for prepubertal girls and patients requiring urgent gonadotoxic therapy. However, the risk of reintroducing malignant cells has prompted the development of safer alternatives, including follicle isolation followed by three-dimensional scaffold encapsulation for transplantation. Fibrin is a promising biomaterial for bioengineered ovary construction, although its ability to support early human follicle maintenance remains unclear. Follicles isolated from cryopreserved ovarian tissues of six patients were encapsulated within fibrin scaffolds of graded concentrations (high, medium, low). After 7 days of in vitro culture, follicle survival and diameter change were quantified. A total of 282 follicles (45.4 \u00b1 10.1 \u00b5m) were embedded into fibrin scaffolds. After culture, 237 viable follicles were detected, yielding an overall survival of 84%. Follicle diameter increased to 58.8 \u00b1 12.0 \u00b5m. Follicle survival rates were comparable across groups, while mean follicle diameter was 56.3 \u00b1 12.5 \u00b5m (high), 61.9 \u00b1 13.4 \u00b5m (medium), and 57.4 \u00b1 9.3 \u00b5m (low). Follicles cultured in medium-concentration fibrin demonstrated significantly larger diameters compared with both high and low groups (p &lt; 0.05), with no difference between high and low groups. Fibrin-based bioprosthetic ovary scaffolds support short-term in vitro maintenance of isolated human follicles, preserving spherical morphology and granulosa cell layer integrity. Medium-concentration fibrin was associated with greater follicle diameter expansion compared with higher and lower concentrations, indicating that scaffold composition influences early morphometric changes during in vitro follicle culture.<\/jats:p>","DOI":"10.3390\/ijms27093799","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T14:28:23Z","timestamp":1777040903000},"page":"3799","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biomimetic Fibrin Matrix Modulates Early Human Follicular Growth Dynamics in a Bioengineered Artificial Ovary Derived from Cryopreserved Tissue: In Vitro Morphometric Assessment"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5660-7074","authenticated-orcid":false,"given":"Mengyang","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3731-6116","authenticated-orcid":false,"given":"Plamen","family":"Todorov","sequence":"additional","affiliation":[{"name":"Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences (BAS), 1113 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Pei","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gohar","family":"Rahimi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"},{"name":"AMEDES Facharzt-Zentrum f\u00fcr Kinderwunsch, Pr\u00e4natale Medizin, Endokrinologie und Osteologie GmbH, 90518 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Skala","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3674-543X","authenticated-orcid":false,"given":"Volodimir","family":"Isachenko","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Medical Faculty, Cologne University, 50931 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.fertnstert.2020.04.050","article-title":"Importance of patient selection to analyze in vitro fertilization outcome with transplanted cryopreserved ovarian tissue","volume":"114","author":"Dolmans","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.1056\/NEJMra1614676","article-title":"Fertility Preservation in Women","volume":"377","author":"Donnez","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/j.molmed.2020.11.003","article-title":"Fertility Preservation: The Challenge of Freezing and Transplanting Ovarian Tissue","volume":"27","author":"Dolmans","year":"2021","journal-title":"Trends Mol. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2186","DOI":"10.1016\/j.fertnstert.2009.11.032","article-title":"Evidence of residual disease in cryopreserved ovarian cortex from female patients with leukemia","volume":"94","author":"Rosendahl","year":"2010","journal-title":"Fertil. Steril."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"363","DOI":"10.2217\/fon-2017-0410","article-title":"Ovarian tissue cryopreservation in young females through the Oncofertility Consortium\u2019s National Physicians Cooperative","volume":"14","author":"Armstrong","year":"2018","journal-title":"Future Oncol."},{"key":"ref_6","first-page":"302","article-title":"Risk of transplanting malignant cells in cryopreserved ovarian tissue","volume":"8","author":"Dolmans","year":"2021","journal-title":"Fertil. Preserv. Princ. Pract."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"F15","DOI":"10.1530\/REP-18-0536","article-title":"Fertility preservation: Construction and use of artificial ovaries","volume":"158","author":"Dolmans","year":"2019","journal-title":"Reproduction"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1111\/aogs.13552","article-title":"From bench to bedside: Current developments and future possibilities of artificial human ovary to restore fertility","volume":"98","author":"Salama","year":"2019","journal-title":"Acta Obstet. Gynecol. Scand."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Cao, M., Todorov, P., Rahimi, G., Salama, M., Woodruff, T.K., Isachenko, E., Skala, C., and Isachenko, V. (2025). Construction and Bioengineering of Human Bioprosthetic Ovaries from Cryopreserved Ovarian Tissue. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26125545"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s13770-016-9107-0","article-title":"Efficient biomaterials for tissue engineering of female reproductive organs","volume":"13","author":"Tamadon","year":"2016","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s10439-022-03125-6","article-title":"Design and application strategies of natural polymer biomaterials in artificial ovaries","volume":"51","author":"Peng","year":"2023","journal-title":"Ann. Biomed. Eng."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1146\/annurev-bioeng-071813-105131","article-title":"Bioengineering the ovarian follicle microenvironment","volume":"16","author":"Shea","year":"2014","journal-title":"Annu. Rev. Biomed. Eng."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/j.fertnstert.2013.12.025","article-title":"A new step toward the artificial ovary: Survival and proliferation of isolated murine follicles after autologous transplantation in a fibrin scaffold","volume":"101","author":"Luyckx","year":"2014","journal-title":"Fertil. Steril."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1089\/ten.tea.2013.0675","article-title":"Fibrin-mediated delivery of an ovarian follicle pool in a mouse model of infertility","volume":"20","author":"Smith","year":"2014","journal-title":"Tissue Eng. Part A"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1093\/humrep\/dew254","article-title":"Influence of follicle stage on artificial ovary outcome using fibrin as a matrix","volume":"31","author":"Chiti","year":"2016","journal-title":"Hum. Reprod."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"17709","DOI":"10.1038\/srep17709","article-title":"Primordial follicle transplantation within designer biomaterial grafts produce live births in a mouse infertility model","volume":"5","author":"Kniazeva","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1093\/molehr\/gax016","article-title":"Further insights into the impact of mouse follicle stage on graft outcome in an artificial ovary environment","volume":"23","author":"Chiti","year":"2017","journal-title":"Mol. Hum. Reprod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.rbmo.2016.05.003","article-title":"Survival and growth of human preantral follicles after cryopreservation of ovarian tissue, follicle isolation and short-term xenografting","volume":"33","author":"Paulini","year":"2016","journal-title":"Reprod. Biomed. Online"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s10815-017-1091-3","article-title":"A novel fibrin-based artificial ovary prototype resembling human ovarian tissue in terms of architecture and rigidity","volume":"35","author":"Chiti","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1016\/j.actbio.2013.09.008","article-title":"Fibrin-based biomaterials: Modulation of macroscopic properties through rational design at the molecular level","volume":"10","author":"Brown","year":"2014","journal-title":"Acta Biomater."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1007\/s10439-017-1817-5","article-title":"Fibrin in Reproductive Tissue Engineering: A Review on Its Application as a Biomaterial for Fertility Preservation","volume":"45","author":"Chiti","year":"2017","journal-title":"Ann. Biomed. Eng."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.actbio.2021.08.026","article-title":"A review on biomaterials for ovarian tissue engineering","volume":"135","author":"Dadashzadeh","year":"2021","journal-title":"Acta Biomater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.fertnstert.2007.11.026","article-title":"Chick embryo chorioallantoic membrane (CAM) model: A useful tool to study short-term transplantation of cryopreserved human ovarian tissue","volume":"91","author":"Donnez","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.fertnstert.2008.05.012","article-title":"Electron paramagnetic resonance as a tool to evaluate human ovarian tissue reoxygenation after xenografting","volume":"92","author":"Jordan","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.fertnstert.2018.04.009","article-title":"In vivo characterization of metabolic activity and oxidative stress in grafted human ovarian tissue using microdialysis","volume":"110","author":"Cacciottola","year":"2018","journal-title":"Fertil. Steril."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"F35","DOI":"10.1530\/REP-19-0097","article-title":"FERTILITY PRESERVATION: Follicle reserve loss in ovarian tissue transplantation","volume":"158","author":"Roness","year":"2019","journal-title":"Reproduction"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s10815-017-1079-z","article-title":"Follicle activation is a significant and immediate cause of follicle loss after ovarian tissue transplantation","volume":"35","author":"Gavish","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1530\/REP-07-0131","article-title":"Short-term transplantation of isolated human ovarian follicles and cortical tissue into nude mice","volume":"134","author":"Dolmans","year":"2007","journal-title":"Reproduction"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s13048-017-0366-8","article-title":"A modified and tailored human follicle isolation procedure improves follicle recovery and survival","volume":"10","author":"Chiti","year":"2017","journal-title":"J. Ovarian Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/S1472-6483(10)60485-3","article-title":"Development of antral follicles after xenografting of isolated small human preantral follicles","volume":"16","author":"Dolmans","year":"2008","journal-title":"Reprod. Biomed. Online"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.2217\/fon-2016-0202","article-title":"The artificial ovary: Current status and future perspectives","volume":"12","author":"Amorim","year":"2016","journal-title":"Future Oncol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s10456-013-9388-z","article-title":"Proangiogenic microtemplated fibrin scaffolds containing aprotinin promote improved wound healing responses","volume":"17","author":"Thomson","year":"2014","journal-title":"Angiogenesis"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e1558","DOI":"10.31661\/gmj.v9i0.1558","article-title":"Fibrin scaffold incorporating platelet lysate enhance follicle survival and angiogenesis in cryopreserved preantral follicle transplantation","volume":"9","author":"Rajabzadeh","year":"2020","journal-title":"Galen Med. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s12013-007-9001-4","article-title":"A comparison of the mechanical and structural properties of fibrin fibers with other protein fibers","volume":"49","author":"Guthold","year":"2007","journal-title":"Cell Biochem. Biophys."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"609","DOI":"10.2217\/rme.14.33","article-title":"First transplantation of isolated murine follicles in alginate","volume":"9","author":"Vanacker","year":"2014","journal-title":"Regen. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1002\/bit.26721","article-title":"Retrievable hydrogels for ovarian follicle transplantation and oocyte collection","volume":"115","author":"Rios","year":"2018","journal-title":"Biotechnol. Bioeng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"16010","DOI":"10.1038\/npjregenmed.2016.10","article-title":"Synthetic hydrogel supports the function and regeneration of artificial ovarian tissue in mice","volume":"1","author":"Kim","year":"2016","journal-title":"NPJ Regen. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1093\/humrep\/dez077","article-title":"Initial steps in reconstruction of the human ovary: Survival of pre-antral stage follicles in a decellularized human ovarian scaffold","volume":"34","author":"Pors","year":"2019","journal-title":"Hum. Reprod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1016\/j.biomaterials.2005.06.016","article-title":"The in vitro regulation of ovarian follicle development using alginate extracellular matrix gels","volume":"27","author":"Kreeger","year":"2006","journal-title":"Biomaterials"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2297","DOI":"10.1016\/j.actbio.2012.02.021","article-title":"Umbilical cord stem cells released from alginate\u2013fibrin microbeads inside macroporous and biofunctionalized calcium phosphate cement for bone regeneration","volume":"8","author":"Chen","year":"2012","journal-title":"Acta Biomater."},{"key":"ref_41","first-page":"529","article-title":"Modification of fibrinogen with poly (ethylene glycol) and its effects on fibrin clot characteristics","volume":"56","author":"Barker","year":"2001","journal-title":"J. Biomed. Mater. Res. Off. J. Soc. Biomater. Jpn. Soc. Biomater. Aust. Soc. Biomater. Korean Soc. Biomater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"B200","DOI":"10.1002\/adem.200980054","article-title":"Proteolytically Degradable Photo-Polymerized Hydrogels Made From PEG\u2013Fibrinogen Adducts","volume":"12","author":"Dikovsky","year":"2010","journal-title":"Adv. Eng. Mater."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"100626","DOI":"10.1016\/j.mtchem.2021.100626","article-title":"Evaluation of PEGylated fibrin as a three-dimensional biodegradable scaffold for ovarian tissue engineering","volume":"22","author":"Dadashzadeh","year":"2021","journal-title":"Mater. Today Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"hoad004","DOI":"10.1093\/hropen\/hoad004","article-title":"Mind the mechanical strength: Tailoring a 3D matrix to encapsulate isolated human preantral follicles","volume":"2023","author":"Dadashzadeh","year":"2023","journal-title":"Hum. Reprod. Open"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Isachenko, V., Todorov, P., Isachenko, E., Rahimi, G., Tchorbanov, A., Mihaylova, N., Manoylov, I., Mallmann, P., and Merzenich, M. (2015). Long-time cooling before cryopreservation decreased translocation of phosphatidylserine (Ptd-L-Ser) in human ovarian tissue. PLoS ONE, 10, Correction in PLoS ONE 2019, 14, e0212961.","DOI":"10.1371\/journal.pone.0212961"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s12958-016-0213-6","article-title":"Cryopreservation and xenografting of human ovarian fragments: Medulla decreases the phosphatidylserine translocation rate","volume":"14","author":"Isachenko","year":"2016","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1089\/bio.2021.0060","article-title":"Optimization of Follicle Isolation for Bioengineering of Human Artificial Ovary","volume":"20","author":"Chen","year":"2022","journal-title":"Biopreserv. Biobank."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Wang, W., Pei, C., Isachenko, E., Zhou, Y., Wang, M., Rahimi, G., Liu, W., Mallmann, P., and Isachenko, V. (2022). Automatic Evaluation for Bioengineering of Human Artificial Ovary: A Model for Fertility Preservation for Prepubertal Female Patients with a Malignant Tumor. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232012419"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0378-4320(03)00088-5","article-title":"The early stages of follicular development: Activation of primordial follicles and growth of preantral follicles","volume":"78","author":"Fortune","year":"2003","journal-title":"Anim. Reprod. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1210\/edrv-17-2-121","article-title":"Regulation of ovarian follicular development in primates: Facts and hypotheses","volume":"17","author":"Gougeon","year":"1996","journal-title":"Endocr. Rev."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/9\/3799\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T04:17:15Z","timestamp":1777177035000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/9\/3799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,24]]},"references-count":50,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["ijms27093799"],"URL":"https:\/\/doi.org\/10.3390\/ijms27093799","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,24]]}}}