{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T01:54:39Z","timestamp":1785462879199,"version":"3.56.0"},"reference-count":39,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82302827"],"award-info":[{"award-number":["82302827"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"award":["82302827"],"award-info":[{"award-number":["82302827"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Guangzhou Science and Technology Plan Project","award":["2025A04J5306"],"award-info":[{"award-number":["2025A04J5306"]}]},{"name":"Project of Administration of Traditional Chinese Medicine of Guangdong Province","award":["20242048"],"award-info":[{"award-number":["20242048"]}]},{"name":"Key Team of Basic and Clinical Research on Tumor Immunotherapy of Guangdong Pharmaceutical University","award":["2024ZZ10"],"award-info":[{"award-number":["2024ZZ10"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Astragalin (AST), a natural flavonoid found in various plants, possesses antioxidant and anti-inflammatory properties. However, its protective efficacy against ultraviolet B (UVB)-induced cutaneous damage remains unclear. This study investigated the photoprotective effects of AST against UVB-induced photodamage using HaCaT keratinocytes and Kunming mice. In vitro, AST mitigated UVB-induced cytotoxicity and apoptosis in HaCaT cells. In vivo, topical application of AST attenuated UVB-induced erythema, epidermal hyperplasia, and collagen degradation in mouse skin. Additionally, AST reduced reactive oxygen species accumulation and enhanced antioxidant enzyme activity via activation of the Keap1\/Nrf2 pathway. Furthermore, AST suppressed the expression of proinflammatory cytokines by inhibiting the TLR4\/NF-\u03baB signaling pathway. These findings demonstrate the photoprotective properties of AST and support its potential as a natural therapeutic agent for preventing UVB-induced skin damage.<\/jats:p>","DOI":"10.3390\/ijms27093839","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:33:37Z","timestamp":1777376017000},"page":"3839","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protective Effects of Astragalin Against Acute Ultraviolet B-Induced Photodamage in HaCaT Cells and Mouse Skin"],"prefix":"10.3390","volume":"27","author":[{"given":"Pei","family":"Tang","sequence":"first","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanhao","family":"Huang","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialing","family":"Tong","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Feng","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Fan","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Ma","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuiling","family":"Qi","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Immunity, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1264","DOI":"10.1111\/php.13634","article-title":"Visible Light and the Skin","volume":"98","author":"Ezekwe","year":"2022","journal-title":"Photochem. 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