{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:54:30Z","timestamp":1785430470067,"version":"3.56.0"},"reference-count":85,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Science and Technology Department of Sichuan Province","doi-asserted-by":"publisher","award":["2025YFHZ0111"],"award-info":[{"award-number":["2025YFHZ0111"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2025YFHZ0111"],"award-info":[{"award-number":["2025YFHZ0111"]}],"id":[{"id":"https:\/\/ror.org\/04323m874","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Plastic pollution is a global environmental crisis, and microbial degradation represents a promising remediation strategy. While bacteria have been widely studied, yeasts offer unique advantages for plastic degradation due to their metabolic versatility, stress tolerance, and enzymatic capabilities. However, plastic degradative yeasts have not been reviewed comprehensively. Although several yeasts capable of degrading polyethylene terephthalate (PET) or polyethylene (PE) have been reported (e.g., Moesziomyces antarcticus, Candida tropicalis, Yarrowia lipolytica and Rhodotorula mucilaginosa), degraders of other plastic types are less studied. Although some yeasts can assimilate carbon from plastics, the diversity of yeasts capable of participating in plastic mineralization remains vastly underexplored. In recent years, yeast cell surface display systems for bacterial PETase and fungal cutinase have been developed, demonstrating promising PET degradation efficiency. However, PETase is feedback-inhibited by the intermediate product mono(2-hydroxyethyl)terephthalate (MHET). Systems synergizing PETase with MHETase have shown superior stability during long-term PET degradation and enable large-scale depolymerization of PET waste. For high-crystallinity PET, fungal hydrophobins can be used to modify the surface hydrophobicity of PETase-displaying yeast cells, facilitating their attachment to hydrophobic PET surfaces and ultimately enhancing the degradation efficiency of the whole-cell biocatalyst. Limitations of current research and future directions are also discussed.<\/jats:p>","DOI":"10.3390\/ijms27093939","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:09:33Z","timestamp":1777388973000},"page":"3939","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Yeast-Mediated Plastic Biodegradation"],"prefix":"10.3390","volume":"27","author":[{"given":"Xin-Yue","family":"Yang","sequence":"first","affiliation":[{"name":"International Science and Technology Cooperation Base for Efficient Utilization of Nutrient Resources and Fertilizer Innovation, College of Resources, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin-Bei","family":"Xie","sequence":"additional","affiliation":[{"name":"International Science and Technology Cooperation Base for Efficient Utilization of Nutrient Resources and Fertilizer Innovation, College of Resources, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong-Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"International Science and Technology Cooperation Base for Efficient Utilization of Nutrient Resources and Fertilizer Innovation, College of Resources, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6565-6914","authenticated-orcid":false,"given":"Shu","family":"Yuan","sequence":"additional","affiliation":[{"name":"International Science and Technology Cooperation Base for Efficient Utilization of Nutrient Resources and Fertilizer Innovation, College of Resources, Sichuan Agricultural University, Chengdu 611130, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1007\/s10311-022-01552-4","article-title":"Biotechnological methods to remove microplastics: A review","volume":"21","author":"Anand","year":"2023","journal-title":"Environ. Chem. Lett."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/j.petsci.2023.08.015","article-title":"Synthetic polymers: A review of applications in drilling fluids","volume":"21","author":"Davoodi","year":"2024","journal-title":"Petrol. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1126\/science.abg5433","article-title":"The global threat from plastic pollution","volume":"373","author":"MacLeod","year":"2021","journal-title":"Science"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"140115","DOI":"10.1016\/j.jhazmat.2025.140115","article-title":"Soil denitrification stimulated by macroplastics in maize cultivation","volume":"499","author":"Zhang","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2617696","DOI":"10.1080\/19490976.2026.2617696","article-title":"Gut microbiome remodeling induced by microplastic exposures in humans","volume":"18","author":"Yang","year":"2026","journal-title":"Gut Microbes"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"108544","DOI":"10.1016\/j.biotechadv.2025.108544","article-title":"Exploring biotechnology for plastic recycling, degradation and upcycling for a sustainable future","volume":"81","author":"Liu","year":"2025","journal-title":"Biotechnol. Adv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1038\/s41564-023-01529-1","article-title":"Sustainable production and degradation of plastics using microbes","volume":"8","author":"Choi","year":"2023","journal-title":"Nat. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1007\/s00203-024-03904-w","article-title":"Microorganism-mediated biodegradation for effective management and\/or removal of micro-plastics from the environment: A comprehensive review","volume":"206","author":"Zahid","year":"2024","journal-title":"Arch. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"140470","DOI":"10.1016\/j.cej.2022.140470","article-title":"Recent advances and challenges in the biotechnological upcycling of plastic wastes for constructing a circular bioeconomy","volume":"454","author":"Lee","year":"2023","journal-title":"Chem. Eng. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1038\/s41586-020-2149-4","article-title":"An engineered PET depolymerase to break down and recycle plastic bottles","volume":"580","author":"Tournier","year":"2020","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Lv, S., Li, Y., Zhao, S., and Shao, Z. (2024). Biodegradation of typical plastics: From microbial diversity to metabolic mechanisms. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25010593"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ekanayaka, A.H., Tibpromma, S., Dai, D., Xu, R., Suwannarach, N., Stephenson, S.L., Dao, C., and Karunarathna, S.C. (2022). A review of the fungi that degrade plastic. J. Fungi, 8.","DOI":"10.3390\/jof8080772"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.1111\/jam.15369","article-title":"Biochemical features and early adhesion of marine Candida parapsilosis strains on high-density polyethylene","volume":"132","author":"Oliveira","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"125659","DOI":"10.1016\/j.cej.2020.125659","article-title":"Mechanistic investigation in Co-biodegradation of phenanthrene and pyrene by Candida tropicalis MTCC 184","volume":"399","author":"Kashyap","year":"2020","journal-title":"Chem. Eng. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"108415","DOI":"10.1016\/j.cep.2021.108415","article-title":"Intensification of pyrene degradation by native Candida tropicalis MTCC 184 with sonication: Kinetic and mechanistic investigation","volume":"164","author":"Kashyap","year":"2021","journal-title":"Chem. Eng. Process."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ahmaditabatabaei, S., Kyazze, G., Iqbal, H.M.N., and Keshavarz, T. (2021). Fungal enzymes as catalytic tools for polyethylene terephthalate (PET) degradation. J. Fungi, 7.","DOI":"10.3390\/jof7110931"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Temporiti, M.E.E., Nicola, L., Nielsen, E., and Tosi, S. (2022). Fungal enzymes involved in plastics biodegradation. Microorganisms, 10.","DOI":"10.3390\/microorganisms10061180"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1002\/jobm.202200428","article-title":"Understanding challenges associated with plastic and bacterial approach toward plastic degradation","volume":"63","author":"Sharma","year":"2023","journal-title":"J. Basic Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1007\/s11274-023-03558-8","article-title":"Solving the plastic dilemma: The fungal and bacterial biodegradability of polyurethanes","volume":"39","author":"Bhavsar","year":"2023","journal-title":"World J.Microbiol.Biotechnol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10945","DOI":"10.1038\/s41598-020-67927-1","article-title":"Fragmentation of plastic objects in a laboratory seawater microcosm","volume":"10","author":"Gerritse","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5016","DOI":"10.1038\/s41598-023-31639-z","article-title":"Aerobic biodegradation of untreated polyester-polyether urethanes by newly isolated yeast strains Exophiliasp. NS-7 and Rhodotorulasp. NS-12","volume":"13","author":"Giyahchi","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1038\/s43705-023-00267-z","article-title":"Polyethylene degradation and assimilation by the marine yeast Rhodotorulamucilaginosa","volume":"3","author":"Vaksmaa","year":"2023","journal-title":"ISME Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"13941","DOI":"10.1007\/s11356-024-31994-y","article-title":"Enhanced biodegradable polyester film degradation in soil by sequential cooperation of yeast-derived esterase and microbial community","volume":"31","author":"Tsuboi","year":"2024","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1007\/s10295-017-1942-z","article-title":"Screening of commercial enzymes for poly(ethylene terephthalate) (PET) hydrolysis and synergy studies on different substrate sources","volume":"44","author":"Carniel","year":"2017","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.procbio.2016.07.023","article-title":"Lipase from Candida antarctica (CALB) and cutinase from Humicolainsolens act synergistically for PET hydrolysis to terephthalic acid","volume":"59","author":"Carniel","year":"2017","journal-title":"Proc. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.1007\/s00449-025-03230-5","article-title":"The use of dimethyl sulfoxide (DMSO) to increase PET microbial degradation by Yarrowialipolytica IMUFRJ 50682","volume":"48","author":"Botelho","year":"2025","journal-title":"Bioprocess Biosyst. Eng."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s13205-025-04396-1","article-title":"Microbial biodegradation of polyethylene terephthalate microplastics by an indigenous Candida tropicalis strain and biocompatibility evaluation of microplastics-degraded metabolites in GIFT Tilapia","volume":"15","author":"Avinash","year":"2025","journal-title":"3 Biotech"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"124106","DOI":"10.1016\/j.envpol.2024.124106","article-title":"Acceleration a yeast-based biodegradation process of polyethylene terephthalate microplastics by Tween 20: Efficiency, by-product analysis, and metabolic pathway prediction","volume":"351","author":"Giyahchi","year":"2024","journal-title":"Environ. Pollut."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Salinas, J., Carpena, V., Mart\u00ednez-Gallardo, M.R., Segado, M., Estrella-Gonz\u00e1lez, M.J., Toribio, A.J., Jurado, M.M., L\u00f3pez-Gonz\u00e1lez, J.A., Su\u00e1rez-Estrella, F., and L\u00f3pez, M.J. (2023). Development of plastic-degrading microbial consortia by induced selection in microcosms. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1143769"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"40437","DOI":"10.1038\/s41598-025-22911-5","article-title":"Functional and genomic characterization of polyethylene degrading yeast Meyerozymacarpophila M6.0.2 isolated from marine plastic debris in East Java Indonesia","volume":"15","author":"Alami","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e0256224","DOI":"10.1128\/aem.02562-24","article-title":"Discovery of two novel cutinases from a gut yeast of plastic-eating mealworm for polyester depolymerization","volume":"91","author":"Huang","year":"2025","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1007\/s10529-007-9354-1","article-title":"Candida rugosa lipase-catalyzed polyurethane degradation in aqueous medium","volume":"29","author":"Gautam","year":"2007","journal-title":"Biotechnol. Lett."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"37757","DOI":"10.1021\/acsomega.2c04734","article-title":"Subcritical hydrothermal liquefaction as a pretreatment for enzymatic degradation of polyurethane","volume":"7","author":"Gallorini","year":"2022","journal-title":"ACS Omega"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7313","DOI":"10.1128\/AEM.02536-13","article-title":"Fungal communities associated with the biodegradation of polyester polyurethane buried under compost at different temperatures","volume":"79","author":"Zafar","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"101111","DOI":"10.1016\/j.margen.2024.101111","article-title":"Complete genome sequencing of Hortaeawerneckii M-3 for identifying polyester polyurethane degrading enzymes","volume":"75","author":"Ling","year":"2024","journal-title":"Mar. Genom."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"135804","DOI":"10.1016\/j.jhazmat.2024.135804","article-title":"Metagenomic analysis of soil from landfill site reveals a diverse microbial community involved in plastic degradation","volume":"480","author":"Kumar","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.jbiotec.2021.03.015","article-title":"Enhancing polypropylene bioconversion and lipogenesis by Yarrowialipolytica using a chemical\/biological hybrid process","volume":"332","author":"Mihreteab","year":"2021","journal-title":"J. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e31130","DOI":"10.1016\/j.heliyon.2024.e31130","article-title":"A screening method for plastic-degrading fungi","volume":"10","author":"Cortizas","year":"2024","journal-title":"Heliyon"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"118677","DOI":"10.1016\/j.envres.2024.118677","article-title":"Effects of polymerization types on plastics ingestion and biodegradation by Zophobasatratus larvae, and successions of both gut bacterial and fungal microbiomes","volume":"251","author":"Weng","year":"2024","journal-title":"Environ. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"El-Dash, H.A., Yousef, N.E., Aboelazm, A.A., Awan, Z.A., Yahya, G., and El-Ganiny, A.M. (2023). Optimizing eco-friendly degradation of polyvinyl chloride (PVC) plastic using environmental strains of Malassezia species and Aspergillus fumigatus. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242015452"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3194","DOI":"10.1128\/AEM.66.8.3194-3200.2000","article-title":"Fungal colonization and biodeterioration of plasticized polyvinyl chloride","volume":"66","author":"Webb","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.1039\/c2gc35876j","article-title":"Influence of chemical and architectural modifications on the enzymatic hydrolysis of poly(butylene succinate)","volume":"14","author":"Gigli","year":"2012","journal-title":"Green Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7548","DOI":"10.1128\/AEM.71.11.7548-7550.2005","article-title":"Cutinase-like enzyme from the yeast Cryptococcus sp.strain S-2 hydrolyzes polylactic acid and other biodegradable plastics","volume":"71","author":"Masaki","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ibiod.2016.03.005","article-title":"Degradation of poly(butylene succinate) and poly(butylene succinate-co-butylene adipate) by a lipase from yeast Cryptococcus sp. grown on agro-industrial residues","volume":"110","author":"Thirunavukarasu","year":"2016","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"105232","DOI":"10.1016\/j.ibiod.2021.105232","article-title":"Efficient biodegradation of aliphatic polyester by genetically engineered strains of the yeast Yarrowialipolytica","volume":"161","author":"Kosiorowska","year":"2021","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Rosato, A., Romano, A., Totaro, G., Celli, A., Fava, F., Zanaroli, G., and Sisti, L. (2022). Enzymatic degradation of the most common aliphatic bio-polyesters and evaluation of the mechanisms involved: An extended study. Polymers, 14.","DOI":"10.3390\/polym14091850"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ru, J., Huo, Y., and Yang, Y. (2020). Microbial degradation and valorization of plastic wastes. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.00442"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1080\/10242420701379734","article-title":"Hydrolysis of PET and bis-(benzoyloxyethyl) terephthalate with a new polyesterase from Penicillium citrinum","volume":"25","author":"Liebminger","year":"2007","journal-title":"Biocatal. Biotransform."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Albayati, S.H., Masomian, M., Ishak, S.N.H., Mohamad Ali, M.S.b., Thean, A.L., Mohd Shariff, F.b., Muhd Noor, N.D.b., and Raja Abd Rahman, R.N.Z. (2020). Main structural targets for engineering lipase substrate specificity. Catalysts, 10.","DOI":"10.3390\/catal10070747"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"7729","DOI":"10.1007\/s00253-019-09999-2","article-title":"Microbial bioconversion of thermally depolymerized polypropylene by Yarrowialipolytica for fatty acid production","volume":"103","author":"Mihreteab","year":"2019","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s40643-022-00532-4","article-title":"Biodegradation of plastic polymers by fungi: A brief review","volume":"9","author":"Srikanth","year":"2022","journal-title":"Bioresour. Bioprocess."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"157358","DOI":"10.1016\/j.scitotenv.2022.157358","article-title":"Production of PETase by engineered Yarrowialipolytica for efficient poly(ethylene terephthalate) biodegradation","volume":"846","author":"Kosiorowska","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1126\/science.aad6359","article-title":"A bacterium that degrades and assimilates poly(ethylene terephthalate)","volume":"351","author":"Yoshida","year":"2016","journal-title":"Science"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3519","DOI":"10.1021\/acscatal.9b00568","article-title":"Rational protein engineering of thermo-stable PETase from Ideonellasakaiensis for highly efficient PET degradation","volume":"9","author":"Son","year":"2019","journal-title":"ACS Catal."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1038\/s41586-022-04599-z","article-title":"Machine learning-aided engineering of hydrolases for PET depolymerization","volume":"604","author":"Lu","year":"2022","journal-title":"Nature"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e202218390","DOI":"10.1002\/anie.202218390","article-title":"Complete depolymerization of PET wastes by an evolved PET hydrolase from directed evolution","volume":"62","author":"Shi","year":"2023","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/s41929-022-00821-3","article-title":"Directed evolution of an efficient and thermostable PET depolymerase","volume":"5","author":"Bell","year":"2022","journal-title":"Nat. Catal."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1002\/cctc.202100443","article-title":"Highly crystalline post-consumer PET waste hydrolysis by surface displayed PETase using a bacterial whole-cell biocatalyst","volume":"13","author":"Gercke","year":"2021","journal-title":"ChemCatChem"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"131177","DOI":"10.1016\/j.biortech.2024.131177","article-title":"Bacterial surface display of PETase mutants and MHETase for an efficient dual-enzyme cascade catalysis","volume":"408","author":"Xue","year":"2024","journal-title":"Bioresour. Technol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e202101932","DOI":"10.1002\/cssc.202101932","article-title":"Comparative performance of PETase as a function of reaction conditions, substrate properties, and product accumulation","volume":"15","author":"Erickson","year":"2022","journal-title":"ChemSusChem"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"134887","DOI":"10.1016\/j.jhazmat.2024.134887","article-title":"Optimization of polyethylene terephthalate biodegradation using a self-assembled multi-enzyme cascade strategy","volume":"476","author":"Aer","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e18228","DOI":"10.1002\/aic.18228","article-title":"Breakdown of polyethylene therepthalate microplastics under saltwater conditions using engineered Vibrio natriegens","volume":"69","author":"Li","year":"2023","journal-title":"AIChE J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"132632","DOI":"10.1016\/j.jhazmat.2023.132632","article-title":"Biodegradation of poly(ethylene terephthalate) through PETase surface-display: From function to structure","volume":"461","author":"Han","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2938","DOI":"10.1021\/acssynbio.1c00175","article-title":"Yeast surface display for in vitro biosynthetic pathway reconstruction","volume":"10","author":"Luo","year":"2021","journal-title":"ACS Synth. Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"139484","DOI":"10.1016\/j.jhazmat.2025.139484","article-title":"Integrated self-assembling multi-enzyme display platform on Candida tropicalis surface for efficient degradation of PET waste","volume":"497","author":"Zhang","year":"2025","journal-title":"J. Hazard. Mater."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"100003","DOI":"10.1016\/j.engmic.2021.100003","article-title":"Potential one-step strategy for PET degradation and PHB biosynthesis through co-cultivation of two engineered microorganisms","volume":"1","author":"Liu","year":"2021","journal-title":"Eng. Microbiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"7138","DOI":"10.1038\/s41467-022-34908-z","article-title":"Biodegradation of highly crystallized poly(ethylene terephthalate) through cell surface codisplay of bacterial PETase and hydrophobin","volume":"13","author":"Chen","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"154841","DOI":"10.1016\/j.scitotenv.2022.154841","article-title":"Metabolic engineering of Yarrowialipolytica for poly(ethylene terephthalate) degradation","volume":"831","author":"Kosiorowska","year":"2022","journal-title":"Sci. Total Environ."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"108475","DOI":"10.1016\/j.abb.2020.108475","article-title":"High-yield expression of extracellular lipase from Yarrowialipolytica and its interactions with lipopeptide biosurfactants: A biophysical approach","volume":"689","author":"Janek","year":"2020","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"129517","DOI":"10.1016\/j.jhazmat.2022.129517","article-title":"Sequentially co-immobilized PET and MHET hydrolases via spy chemistry in calcium phosphate nanocrystals present high-performance PET degradation","volume":"438","author":"Chen","year":"2022","journal-title":"J. Hazard. Mater."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"135380","DOI":"10.1016\/j.jhazmat.2024.135380","article-title":"A customized self-assembled synergistic biocatalyst for plastic depolymerization","volume":"477","author":"Zhang","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1002\/bit.28963","article-title":"High-efficiency PET degradation with a duo-enzyme system immobilized on magnetic nanoparticles","volume":"122","author":"Kotnis","year":"2025","journal-title":"Biotechnol. Bioeng."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1038\/nature08264","article-title":"Surface hydrophobin prevents immune recognition of airborne fungal spores","volume":"460","author":"Aimanianda","year":"2009","journal-title":"Nature"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.cocis.2009.04.001","article-title":"Hydrophobins: Proteins that self assemble at interfaces","volume":"14","author":"Linder","year":"2009","journal-title":"Curr. Opin. Colloid Interface Sci."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"5848","DOI":"10.1002\/j.1460-2075.1994.tb06929.x","article-title":"Interfacial selfassembly of a hydrophobin into an amphipathic protein membrane mediates fungal attachment to hydrophobic surfaces","volume":"13","author":"Schuren","year":"1994","journal-title":"EMBO J."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"4293","DOI":"10.1021\/la300501u","article-title":"Self-assembly of class II hydrophobins on polar surfaces","volume":"28","author":"Szilvay","year":"2012","journal-title":"Langmuir"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.ijbiomac.2021.10.089","article-title":"Class I hydrophobin fusion with cellulose binding domain for its soluble expression and facile purification","volume":"193","author":"Puspitasari","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1007\/s12010-020-03358-y","article-title":"Fungal hydrophobin RolA enhanced PETase hydrolysis of polyethylene terephthalate","volume":"193","author":"Puspitasari","year":"2021","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1038\/356615a0","article-title":"Fusarium solanicutinase is a lipolytic enzyme with a catalytic serine accessible to solvent","volume":"356","author":"Martinez","year":"1992","journal-title":"Nature"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"eadl2746","DOI":"10.1126\/science.adl2746","article-title":"Twenty years of microplastic pollution research-what have we learned?","volume":"386","author":"Thompson","year":"2024","journal-title":"Science"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Mohanan, N., Montazer, Z., Sharma, P.K., and Levin, D.B. (2020). Microbial and enzymatic degradation of synthetic plastics. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.580709"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1002\/yea.3827","article-title":"Oleaginous yeasts: Time to rethink the definition?","volume":"39","author":"Vandeputte","year":"2022","journal-title":"Yeast"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"6963","DOI":"10.1007\/s00253-022-12208-2","article-title":"Papiliotremalaurentii: General features and biotechnological applications","volume":"106","author":"Ventorim","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1002\/biot.201600008","article-title":"A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films","volume":"11","author":"Barth","year":"2016","journal-title":"Biotechnol. J."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.ijbiomac.2022.11.126","article-title":"A versatile tag for simple preparation of cutinase towards enhanced biodegradation of polyethylene terephthalate","volume":"225","author":"Yuan","year":"2023","journal-title":"Int. J. Biol. Macromol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/9\/3939\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T04:16:01Z","timestamp":1778127361000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/9\/3939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,28]]},"references-count":85,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["ijms27093939"],"URL":"https:\/\/doi.org\/10.3390\/ijms27093939","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,28]]}}}