{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:13:36Z","timestamp":1790856816078,"version":"4.1.0"},"reference-count":99,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001287","name":"Elsa U. Pardee Foundation","doi-asserted-by":"publisher","award":["273383"],"award-info":[{"award-number":["273383"]}],"id":[{"id":"10.13039\/100001287","id-type":"DOI","asserted-by":"publisher"}]},{"award":["273383"],"award-info":[{"award-number":["273383"]}],"id":[{"id":"https:\/\/ror.org\/05sjnvv07","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Mitchell Cancer Institute and Frederick P. Whiddon College of Medicine, The University of South Alabama"},{"id":[{"id":"https:\/\/ror.org\/01s7b5y08","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cervical cancer (CC) is an alarming global health problem, with predominantly higher incidence, lethal progression, and mortality among women of African ancestry (AA) than women of European ancestry (EA). Although persistent high-risk human papillomavirus (HPV) integration and infection are the key etiological factors, currently available evidence implicates epigenetic reprogramming as a prime contributor to ancestry-associated differences in CC pathogenesis. To address these disparities, we performed genome-wide DNA methylation profiling of HPV-positive cervical intraepithelial neoplasia (CIN) lesions from AA (n = 15) and EA (n = 15) women. Differential methylation analysis identified a distinct epigenomic landscape in AA-CIN lesions, with widespread hypermethylation and hypomethylation at promoter-associated and regulatory CpG sites. Pathway enrichment analyses highlighted dysregulation of ECM-receptor interaction, focal adhesion, PI3K-Akt, MAPK, Ras, Rap1, and RUNX-dependent transcriptional networks. Comparative analysis across CIN grades (CIN1\u2013CIN3) revealed progressive epigenetic reprogramming affecting cell cycles, cytoskeletal dynamics, signaling, and metabolic pathways. Among hypermethylated tumor suppressor genes, SH3GL2 and ARHGAP25 showed significantly higher methylation in AA lesions, accompanied by concomitant loss of their protein expression. MBD1, a methylation-binding regulator, was upregulated in AA-CIN lesions, coinciding with global loss of 5-hydroxymethylcytosine (5hmC), suggesting enhanced transcriptional repression. In contrast, EA lesions retained protein expression and 5hmC levels. Collectively, these findings indicate that early, ancestry-specific epigenetic modifications target tumor suppressor pathways and converge on oncogenic signaling, cytoskeletal remodeling, and cell\u2013cell adhesion. Our study provides mechanistic insight into CC health disparities, identifying SH3GL2 and ARHGAP25 hypermethylation as potential biomarkers, and highlighting epigenetic regulation as a contributor to disparate CC progression in AA women.<\/jats:p>","DOI":"10.3390\/ijms27093986","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:01:18Z","timestamp":1777474878000},"page":"3986","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genome-Wide DNA Methylation Profiling Reveals Ancestry-Associated Epigenetic Reprogramming in Cervical Intraepithelial Neoplasia"],"prefix":"10.3390","volume":"27","author":[{"given":"Mohamed","family":"Masoud","sequence":"first","affiliation":[{"name":"Department of Pathology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL 36617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charu","family":"Shastri","sequence":"additional","affiliation":[{"name":"Department of Pathology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL 36617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajarshi","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Pathology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL 36617, USA"},{"name":"Mitchell Cancer Institute, University of South Alabama, Mobile, AL 36604, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saanvi","family":"Dasgupta","sequence":"additional","affiliation":[{"name":"Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector","family":"Chavarria-Bernal","sequence":"additional","affiliation":[{"name":"Department of Pathology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL 36617, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karan P.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics, The University of Texas at Tyler Health Science Center, Tyler, TX 75708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer Y.","family":"Pierce","sequence":"additional","affiliation":[{"name":"Mitchell Cancer Institute, University of South Alabama, Mobile, AL 36604, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1499-0039","authenticated-orcid":false,"given":"Santanu","family":"Dasgupta","sequence":"additional","affiliation":[{"name":"Department of Pathology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL 36617, USA"},{"name":"Mitchell Cancer Institute, University of South Alabama, Mobile, AL 36604, USA"},{"name":"Department of Biochemistry and Molecular Biology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL 36688, USA"},{"name":"Department of Urology, Frederick P. Whiddon College of Medicine, University of South Alabama, Mobile, AL 36688, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"ref_1","first-page":"322","article-title":"Global Burden of Cervical Cancer: Current Estimates, Temporal Trend and Future Projections Based on the GLOBOCAN 2022","volume":"5","author":"Wu","year":"2025","journal-title":"J. Natl. Cancer Cent."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Huang, Y., Lin, W., Chen, X., Zheng, X., Yi, H., and Zhang, L. (J. Adv. Res., 2025). 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