{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:06:15Z","timestamp":1779339975345,"version":"3.51.4"},"reference-count":59,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Ministry of Trade, Industry &amp; Energy","award":["20020268"],"award-info":[{"award-number":["20020268"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Obesity is associated with excessive lipid deposition in adipose tissue and the liver, leading to systemic metabolic disturbances. In this study, we investigated the anti-obesity efficacy of Tisochrysis lutea (TL) powder, standardized to fucoxanthin (12.18 \u00b1 0.21 mg\/g DW) and docosahexaenoic acid (DHA) (16.03 \u00b1 0.49 mg\/g DW), in a high-fat diet (HFD)-induced obesity model in C57BL\/6N mice. TL supplementation (50\u2013150 mg\/kg) over eight weeks significantly reduced body weight gain by up to 63.2%, total white adipose tissue mass by 53.4%, and liver weight by 38.2% compared to the HFD control, without affecting renal safety markers. Histological examination revealed smaller adipocytes and diminished hepatic steatosis in TL-treated groups. Serum triglycerides and leptin concentrations were significantly lowered by 38.5% and 70.1%, respectively, while HFD-induced elevations of ALT and AST were reduced by 61.7% and 38.6%, respectively. At the transcriptional level, TL downregulated adipogenic markers including PPAR\u03b3, C\/EBP\u03b1, and SREBP-1c by 46~54%, as well as lipogenic regulators including FAS and ACC1 by up to 72%. Furthermore, TL treatment upregulated the mRNA levels of HSL and AMPK 2.3- and 2.1-fold, respectively, compared to the HFD control. These findings indicate that fucoxanthin- and DHA-enriched TL powder improves obesity-related metabolic alterations by modulating lipid storage and utilization pathways, supporting its development as a marine-derived functional ingredient for metabolic health management.<\/jats:p>","DOI":"10.3390\/ijms27104277","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:49:09Z","timestamp":1778572149000},"page":"4277","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anti-Obesity Effects of Tisochrysis lutea Powder in High-Fat Diet-Induced Obese Mice Through the Regulation of Adipogenesis and Lipid Metabolism"],"prefix":"10.3390","volume":"27","author":[{"given":"Jae-In","family":"Eom","sequence":"first","affiliation":[{"name":"Microalgae Ask Us Co., Ltd., Gangneung 25441, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Se-Min","family":"Kim","sequence":"additional","affiliation":[{"name":"Microalgae Ask Us Co., Ltd., Gangneung 25441, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joo Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Microalgae Ask Us Co., Ltd., Gangneung 25441, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji-Woo","family":"Kim","sequence":"additional","affiliation":[{"name":"Microalgae Ask Us Co., Ltd., Gangneung 25441, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8144-014X","authenticated-orcid":false,"given":"Dae Yoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biology Education, College of Education, Chungbuk National University, Cheongju 28644, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Kwon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biology Education, College of Education, Chungbuk National University, Cheongju 28644, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheol-Ho","family":"Pan","sequence":"additional","affiliation":[{"name":"Microalgae Ask Us Co., Ltd., Gangneung 25441, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1111\/obr.12551","article-title":"Obesity: A chronic relapsing progressive disease process. 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