{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T05:08:53Z","timestamp":1779167333876,"version":"3.51.4"},"reference-count":50,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFA1108301"],"award-info":[{"award-number":["2024YFA1108301"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018542","name":"Sichuan Natural Science Foundation","doi-asserted-by":"publisher","award":["2024ZYD0185"],"award-info":[{"award-number":["2024ZYD0185"]}],"id":[{"id":"10.13039\/501100018542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Long non-coding RNAs (lncRNAs) have emerged as important regulators of developmental processes. Recent studies have established roles for lncRNAs in human and murine erythroid regulation, yet additional regulators remain to be discovered. To identify lncRNA candidates involved in human erythroid regulation, we established a pooled genome-editing screen strategy using human embryonic stem cells (hESCs). Long Intergenic Non-Protein Coding RNA 1089 (LINC01089) was selected for functional investigation. We found that reduced LINC01089 expression impaired erythroid differentiation. Transcriptomic profiling revealed consistent downregulation of genes related to hemoglobin assembly, heme biosynthesis, and membrane maturation, suggesting that LINC01089 supports coordinated erythroid transcriptional programs. In particular, progressive reduction of HBB expression emerged as a key transcriptional anchor. Enrichment analyses of upregulated genes identified recurrent focal adhesion signatures, suggesting a potential link between LINC01089 and focal adhesion kinase (FAK)-related signaling. Given prior evidence linking LINC01089 to FAK phosphorylation, we performed a pilot FAK-inhibition experiment, producing a partial shift toward wild-type HBB expression and supporting FAK\/phosphorylated FAK (pFAK) signaling as a potential contributing axis in the impaired transcriptional programs. Together, our findings identify LINC01089 as a novel lncRNA linked to coordinated heme\u2013globin transcriptional programs in human erythroid differentiation, with possible involvement of the FAK\/pFAK axis.<\/jats:p>","DOI":"10.3390\/ijms27104394","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:27:56Z","timestamp":1778776076000},"page":"4394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduced LINC01089 Expression Impairs Coordinated Heme\u2013Globin Transcriptional Programs in Human Erythroid Cells"],"prefix":"10.3390","volume":"27","author":[{"given":"Yuan","family":"Xue","sequence":"first","affiliation":[{"name":"Center of Stem Cell and Regenerative Medicine, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohong","family":"Li","sequence":"additional","affiliation":[{"name":"Center of Stem Cell and Regenerative Medicine, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yonggang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center of Stem Cell and Regenerative Medicine, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Ma","sequence":"additional","affiliation":[{"name":"Center of Stem Cell and Regenerative Medicine, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu 610052, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6777","DOI":"10.1038\/sj.onc.1210761","article-title":"Transcriptional Control of Erythropoiesis: Emerging Mechanisms and Principles","volume":"26","author":"Kim","year":"2007","journal-title":"Oncogene"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1182\/blood-2011-04-285981","article-title":"Transcription Factor Networks in Erythroid Cell and Megakaryocyte Development","volume":"118","author":"Crispino","year":"2011","journal-title":"Blood"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Wells, M., and Steiner, L. 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