{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:52:37Z","timestamp":1784865157892,"version":"3.55.0"},"reference-count":63,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:00:00Z","timestamp":1778803200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cardiac surgery-associated acute kidney injury (CSA-AKI) is a frequent and severe complication of open-heart surgery. Although oxidative\/inflammatory mechanisms are known to contribute to its pathophysiology, the circulating factors involved are poorly understood. In this preliminary investigation, we evaluated the effects of plasma from patients undergoing cardiac surgery on endothelial and renal tubular cells at anesthesia induction (T0) and 48 h after surgery (T1). Plasma levels of thiobarbituric acid-reactive substances (TBARSs), glutathione (GSH), and nitric oxide (NO) were measured in parallel. At T0, patient plasma showed increased TBARSs and reduced GSH and NO levels, consistent with oxidative imbalance, and induced cellular injury. In both cell types, plasma exposure reduced cell viability and mitochondrial membrane potential, while it increased oxidant release. Endothelial cells also showed decreased NO production, whereas renal tubular displayed increased apoptotic markers and reduced anti-aging factors. At T1, these alterations were further aggravated only in patients who developed CSA-AKI, whose plasma caused more severe endothelial and tubular damage. These findings support the presence of circulating injurious factors in cardiac surgery patient plasma that may contribute to CSA-AKI pathogenesis and help identify patients at risk before irreversible kidney damage develops.<\/jats:p>","DOI":"10.3390\/ijms27104416","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:40:31Z","timestamp":1779115231000},"page":"4416","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Plasma from Cardiac Surgery Patients Induces Endothelial and Tubular Epithelial Cell Damage: Potential Role in Acute Kidney Injury Development\u2014A Preliminary Report"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3012-259X","authenticated-orcid":false,"given":"Elena","family":"Grossini","sequence":"first","affiliation":[{"name":"Laboratory of Physiology, Department of Translational Medicine, Universit\u00e0 Del Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2583-6433","authenticated-orcid":false,"given":"Teresa","family":"Esposito","sequence":"additional","affiliation":[{"name":"Cardiac Anesthesia and Intensive Care Unit, Azienda Ospedaliero Universitaria Maggiore Della Carit\u00e0, Corso Mazzini 18, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7517-2574","authenticated-orcid":false,"given":"Sakthipriyan","family":"Venkatesan","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology, Department of Translational Medicine, Universit\u00e0 Del Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8810-4688","authenticated-orcid":false,"given":"Mohammad Mostafa","family":"Ola 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Kidney J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1186\/s13054-016-1352-z","article-title":"Acute kidney injury following cardiac surgery: Current understanding and future directions","volume":"20","author":"Shaw","year":"2016","journal-title":"Crit. Care"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Wang, X.D., Bao, R., Lan, Y., Zhao, Z.Z., Yang, X.Y., Wang, Y.Y., Quan, Z.Y., Wang, J.F., and Bian, J.J. (2024). The incidence, risk factors, and prognosis of acute kidney injury in patients after cardiac surgery. Front. Cardiovasc. Med., 11.","DOI":"10.3389\/fcvm.2024.1396889"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Liu, C., Zhang, H.T., Yue, L.J., Li, Z.S., Pan, K., Chen, Z., Gu, S.P., Pan, T., Pan, J., and Wang, D.J. (2021). Risk factors for mortality in patients undergoing continuous renal replacement therapy after cardiac surgery. BMC Cardiovasc. Disord., 21.","DOI":"10.1186\/s12872-021-02324-8"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Duchnowski, P., and \u015amigielski, W. (2024). Risk Factors for Postoperative Acute Kidney Injury Requiring Renal Replacement Therapy in Patients Undergoing Heart Valve Surgery. J. Clin. Med., 13.","DOI":"10.3390\/jcm13247811"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Schurle, A., and Koyner, J.L. (2021). CSA-AKI: Incidence, Epidemiology, Clinical Outcomes, and Economic Impact. J. Clin. Med., 10.","DOI":"10.3390\/jcm10245746"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s13613-025-01424-4","article-title":"Impact of mechanical ventilation on severe acute kidney injury in critically ill patients with and without COVID-19\u2014A multicentre propensity matched analysis","volume":"15","author":"Perschinka","year":"2025","journal-title":"Ann. Intensive Care"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chao, C.T., Hou, C.C., Wu, V.C., Lu, H.M., Wang, C.Y., Chen, L., and Kao, T.W. (2012). The impact of dialysis-requiring acute kidney injury on long-term prognosis of patients requiring prolonged mechanical ventilation: Nationwide population-based study. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0050675"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1007\/s40620-025-02234-4","article-title":"Epidemiology, trajectories and outcomes of acute kidney injury among hospitalized patients: A large retrospective multicenter cohort study","volume":"38","author":"Adiyeke","year":"2025","journal-title":"J. Nephrol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"gfaf093","DOI":"10.1093\/ndt\/gfaf093","article-title":"Long-term risks of adverse kidney outcomes after acute kidney injury: A systematic review and meta-analysis","volume":"40","author":"Veltkamp","year":"2025","journal-title":"Nephrol. Dial. Transplant."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1016\/j.jtcvs.2020.01.101","article-title":"Costs and consequences of acute kidney injury after cardiac surgery: A cohort study","volume":"162","author":"Lau","year":"2021","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Jiang, M., and Wang, T. (2024). Reactive oxygen species (ROS)-responsive biomaterials for treating myocardial ischemia-reperfusion injury. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1469393"},{"key":"ref_13","first-page":"62","article-title":"An Overview of Ischemia-Reperfusion Injury: Review on Oxidative Stress and Inflammatory Response","volume":"54","author":"Tanyeli","year":"2022","journal-title":"Eurasian J. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1007\/s40256-024-00715-8","article-title":"Kidney Injury Following Cardiac Surgery: A Review of Our Current Understanding","volume":"25","author":"Kamla","year":"2025","journal-title":"Am. J. Cardiovasc. Drugs"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.1039\/D3TB02318D","article-title":"ROS-responsive curcumin-encapsulated nanoparticles for AKI therapy via promoting lipid degradation in renal tubules","volume":"12","author":"Guo","year":"2024","journal-title":"J. Mater. Chem. B"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1016\/j.jtcvs.2020.03.182","article-title":"Nitric oxide delivery during cardiopulmonary bypass reduces acute kidney injury: A randomized trial","volume":"163","author":"Kamenshchikov","year":"2022","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zhang, J., Zhang, H., Wang, J., Wang, T., Bian, L., Li, Z., Wang, J., Teng, Y., Liu, G., and Yan, S. (2025). The organ-protective effects of nitric oxide in adult patients undergoing cardiac surgery with cardiopulmonary bypass: A systematic review and meta-analysis. BMC Anesthesiol., 25.","DOI":"10.1186\/s12871-025-03207-7"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1097\/TP.0000000000004038","article-title":"DAMPs Released From Injured Renal Tubular Epithelial Cells Activate Innate Immune Signals in Healthy Renal Tubular Epithelial Cells","volume":"106","author":"DeWolf","year":"2022","journal-title":"Transplantation"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2183026","DOI":"10.1155\/2016\/2183026","article-title":"ROS-Mediated NLRP3 Inflammasome Activation in Brain, Heart, Kidney, and Testis Ischemia\/Reperfusion Injury","volume":"2016","author":"Minutoli","year":"2016","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"909","DOI":"10.34067\/KID.0000000000000466","article-title":"Cardiac Surgery-Associated Acute Kidney Injury","volume":"5","author":"Scurt","year":"2024","journal-title":"Kidney360"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1038\/nrneph.2017.119","article-title":"Cardiac surgery-associated acute kidney injury: Risk factors, pathophysiology and treatment","volume":"13","author":"Wang","year":"2017","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"S117","DOI":"10.1016\/j.kint.2023.10.018","article-title":"KDIGO 2024 Clinical Practice Guideline for the Evaluation and Management of Chronic Kidney Disease","volume":"105","author":"Stevens","year":"2024","journal-title":"Kidney Int."},{"key":"ref_23","first-page":"120","article-title":"Acute kidney injury after open-heart surgery procedures","volume":"60","author":"Velicki","year":"2021","journal-title":"Acta Clin. Croat."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1186\/s13613-024-01360-9","article-title":"Biomarkers in acute kidney injury","volume":"14","author":"Ostermann","year":"2024","journal-title":"Ann. Intensive Care"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1186\/s13054-022-04223-6","article-title":"Comparative accuracy of biomarkers for the prediction of hospital-acquired acute kidney injury: A systematic review and meta-analysis","volume":"26","author":"Pan","year":"2022","journal-title":"Crit. Care"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"137","DOI":"10.3892\/mmr.2025.13503","article-title":"Efficacy of exosomes in acute kidney injury treatment and the associated mechanism (Review)","volume":"31","author":"Zhang","year":"2025","journal-title":"Mol. Med. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1159\/000531291","article-title":"Renal proximal tubular epithelial cells: From harvesting to use in studies","volume":"147","author":"Petreski","year":"2023","journal-title":"Nephron"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1186\/s13643-022-02003-5","article-title":"Extracellular vesicles for ischemia\/reperfusion injury-induced acute kidney injury: A systematic review and meta-analysis of data from animal models","volume":"11","author":"Li","year":"2022","journal-title":"Syst. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1007\/s00134-015-3934-7","article-title":"Epidemiology of acute kidney injury in critically ill patients: The multinational AKI-EPI study","volume":"41","author":"Hoste","year":"2015","journal-title":"Intensive Care Med."},{"key":"ref_30","first-page":"1345","article-title":"A review of the pathophysiology and current management of cardiac surgery-associated acute kidney injury","volume":"102","author":"Shaw","year":"2016","journal-title":"Ann. Thorac. Surg."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Meersch, M., Schmidt, C., Van Aken, H., Martens, S., Rossaint, J., Singbartl, K., G\u00f6rlich, D., Kellum, J.A., and Zarbock, A. (2014). Urinary TIMP-2 and IGFBP7 as early biomarkers of acute kidney injury and renal recovery following cardiac surgery. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0093460"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2331062","DOI":"10.1080\/0886022X.2024.2331062","article-title":"Cardiopulmonary bypass associated acute kidney injury: Better understanding and better prevention","volume":"46","author":"Yang","year":"2024","journal-title":"Ren. Fail."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Vermeulen Windsant, I.C., de Wit, N.C.J., Sertorio, J.T.C., van Bijnen, A.A., Ganushchak, Y.M., Heijmans, J.H., Tanus-Santos, J.E., Jacobs, M.J., Maessen, J.G., and Buurman, W.A. (2014). Hemolysis during cardiac surgery is associated with increased intravascular nitric oxide consumption and perioperative kidney injury. Front. Physiol., 5.","DOI":"10.3389\/fphys.2014.00340"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1159\/000363203","article-title":"The microcirculation in acute kidney injury","volume":"127","author":"Ince","year":"2014","journal-title":"Nephron Clin. Pract."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"F145","DOI":"10.1152\/ajprenal.00164.2016","article-title":"The proximal tubule is the primary target of injury and progression of kidney disease: Role of the glomerulotubular junction","volume":"311","author":"Chevalier","year":"2016","journal-title":"Am. J. Physiol. Ren. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"155088","DOI":"10.1016\/j.jcrc.2025.155088","article-title":"Low postoperative mitochondrial oxygen tension is an early marker of acute kidney injury after cardiac surgery: A prospective observational study","volume":"88","author":"Hilderink","year":"2025","journal-title":"J. Crit. Care"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1038\/nm1613","article-title":"Endothelial-to-mesenchymal transition contributes to cardiac fibrosis","volume":"13","author":"Zeisberg","year":"2007","journal-title":"Nat. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1038\/nrneph.2012.92","article-title":"Klotho as a potential biomarker and therapy for acute kidney injury","volume":"8","author":"Hu","year":"2012","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_39","first-page":"7351","article-title":"Klotho: A novel and early biomarker of acute kidney injury after cardiac valve replacement surgery in adults","volume":"8","author":"Liu","year":"2015","journal-title":"Int. J. Clin. Exp. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1681\/ASN.2017111218","article-title":"Sirtuins in renal health and disease","volume":"29","author":"Morigi","year":"2018","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1016\/j.kint.2016.08.023","article-title":"Transcription factor Nrf2 hyperactivation in early-phase renal ischemia-reperfusion injury prevents tubular damage progression","volume":"91","author":"Nezu","year":"2017","journal-title":"Kidney Int."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Cappucci, I.P., Tremoli, E., Zavan, B., and Ferroni, L. (2025). Circulating extracellular vesicles in cardiovascular disease. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26146817"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Martin-Ventura, J.L., Roncal, C., Orbe, J., and Blanco-Colio, L.M. (2022). Role of extracellular vesicles as potential diagnostic and\/or therapeutic biomarkers in chronic cardiovascular diseases. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.813885"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"6271","DOI":"10.2147\/IJN.S519345","article-title":"Extracellular vesicles in acute kidney injury: Mechanisms, biomarkers, and therapeutic potential","volume":"20","author":"Yan","year":"2025","journal-title":"Int. J. Nanomed."},{"key":"ref_45","first-page":"e51228","article-title":"Acute kidney injury: Definition, management, and promising therapeutic target","volume":"15","author":"Almazmomi","year":"2023","journal-title":"Cureus"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1053\/j.jvca.2025.11.009","article-title":"Acute kidney injury in cardiac surgery: A comprehensive review of perioperative strategies and emerging biomarkers","volume":"40","author":"Cuttone","year":"2026","journal-title":"J. Cardiothorac. Vasc. Anesth."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Li, X., Yuan, F., and Zhou, L. (2022). Organ crosstalk in acute kidney injury: Evidence and mechanisms. J. Clin. Med., 11.","DOI":"10.3390\/jcm11226637"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"112078","DOI":"10.1016\/j.mad.2025.112078","article-title":"Functional significance of extracellular vesicles as mediators of cardiometabolic and cardiorenal diseases upon aging","volume":"226","author":"Chung","year":"2025","journal-title":"Mech. Ageing Dev."},{"key":"ref_49","first-page":"505","article-title":"Mitochondrial oxygenation monitoring and acute kidney injury risk in cardiac surgery: A prospective cohort study","volume":"38","author":"Streng","year":"2024","journal-title":"J. Clin. Monit. Comput."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1056\/NEJMra1214243","article-title":"Acute kidney injury and chronic kidney disease as interconnected syndromes","volume":"371","author":"Chawla","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1094","DOI":"10.1016\/j.athoracsur.2017.12.034","article-title":"Preoperative Renal Functional Reserve Predicts Risk of Acute Kidney Injury After Cardiac Operation","volume":"105","author":"Ferrari","year":"2018","journal-title":"Ann. Thorac. Surg."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1093\/ndt\/gfy227","article-title":"Persistent decrease of renal functional reserve in patients after cardiac surgery-associated acute kidney injury despite clinical recovery","volume":"34","author":"Ferrari","year":"2019","journal-title":"Nephrol. Dial. Transplant."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1681\/ASN.2004040331","article-title":"A Clinical Score to Predict Acute Renal Failure after Cardiac Surgery","volume":"16","author":"Thakar","year":"2005","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Grossini, E., Garhwal, D., Venkatesan, S., Ferrante, D., Mele, A., Saraceno, M., Scognamiglio, A., Mandrioli, J., Amedei, A., and De Marchi, F. (2022). The Potential Role of Peripheral Oxidative Stress on the Neurovascular Unit in Amyotrophic Lateral Sclerosis Pathogenesis: A Preliminary Report from Human and In Vitro Evaluations. Biomedicines, 10.","DOI":"10.3390\/biomedicines10030691"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Grossini, E., Venkatesan, S., Ola Pour, M.M., Ferrante, D., Surico, D., Vaschetto, R., Cantaluppi, V., and Pirisi, M. (2024). Chromogranin B Protects Human Umbilical Endothelial Cells against Oxidative Stress. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms251910296"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Grossini, E., De Marchi, F., Venkatesan, S., Mele, A., Ferrante, D., and Mazzini, L. (2023). Effects of Acetyl-L-Carnitine on Oxidative Stress in Amyotrophic Lateral Sclerosis Patients: Evaluation on Plasma Markers and Members of the Neurovascular Unit. Antioxidants, 12.","DOI":"10.3390\/antiox12101887"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Grossini, E., Bellan, M., Venkatesan, S., Ola Pour, M.M., Mennuni, M., D\u2019Amario, D., Bruno, S., Ferrante, D., Capello, D., and Sainaghi, P.P. (2025). Characterization of Circulating Vesicles of Complicated and Uncomplicated Systemic Sclerosis Patients and Their Role in Vascular Dysfunction. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26062380"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Grossini, E., Esposito, T., Viretto, M., Venkatesan, S., Licari, I., Surico, D., Della Corte, F., Castello, L., Bruno, S., and Quaglia, M. (2023). Circulating Extracellular Vesicles in Subarachnoid Hemorrhage Patients: Characterization and Cellular Effects. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241914913"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Grossini, E., Smirne, C., Venkatesan, S., Tonello, S., D\u2019Onghia, D., Minisini, R., Cantaluppi, V., Sainaghi, P.P., Comi, C., and Tanzi, A. (2023). Plasma Pattern of Extracellular Vesicles Isolated from Hepatitis C Virus Patients and Their Effects on Human Vascular Endothelial Cells. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241210197"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Grossini, E., Venkatesan, S., Alkabes, M., Toma, C., and de Cill\u00e0, S. (2022). Membrane Blue Dual Protects Retinal Pigment Epithelium Cells\/Ganglion Cells-Like through Modulation of Mitochondria Function. Biomedicines, 10.","DOI":"10.3390\/biomedicines10112854"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Grossini, E., Quaglia, M., Prenna, S., Stasi, A., Franzin, R., Castellano, G., Remorgida, V., Libretti, A., Venkatesan, S., and Smirne, C. (2025). Plasma Extracellular Vesicles from Preeclamptic Patients Trigger a Detrimental Crosstalk Between Glomerular Endothelial Cells and Podocytes Involving Endothelin-1. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26114962"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1007\/s40620-020-00943-6","article-title":"Citrate high volume on-line hemodiafiltration modulates serum Interleukin-6 and Klotho levels: The multicenter randomized controlled study \u201cHephaestus\u201d","volume":"34","author":"Pizzarelli","year":"2021","journal-title":"J. Nephrol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"603","DOI":"10.17219\/acem\/121519","article-title":"Annexin V in children with idiopathic nephrotic syndrome treated with cyclosporine A","volume":"29","author":"Jakubowska","year":"2020","journal-title":"Adv. Clin. Exp. 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