{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:29:20Z","timestamp":1785403760496,"version":"3.56.0"},"reference-count":25,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T00:00:00Z","timestamp":1778889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Direcci\u00f3n General de Investigaciones of Universidad Santiago de Cali","award":["DGI-01-2026"],"award-info":[{"award-number":["DGI-01-2026"]}]},{"DOI":"10.13039\/501100003402","name":"Fundaci\u00f3n Valle del Lili","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003402","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/00xdnjz02","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Escuela Nacional del Deporte"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Breast cancer represents a major public health problem worldwide. Despite radical surgery for localized disease, a substantial proportion of patients experience disease recurrence. The aim of this study was to evaluate the expression of the mammaglobin and CK19 genes in sentinel lymph node biopsies from patients with early breast cancer. This descriptive study included 301 sentinel lymph node biopsies from patients with stage I\u2013II breast cancer treated at the San Carlos Clinical Hospital in Madrid, Spain. Metastases were identified using conventional histopathology (H&amp;E), immunohistochemistry (IHC), and molecular detection of mammaglobin and CK19 using PCR-based methods. Associations between variables were assessed using Fisher\u2019s exact test with a 95% confidence level. Statistical analyses were performed using STATA 12.0. The predictive value for metastatic involvement was 12.29% for CK19 and 16.61% for mammaglobin, increasing to 19.27% when conventional staining was combined with immunohistochemistry. The overall sensitivity was 68.9%, and the specificity was 93.42%. Mammaglobin showed slightly better diagnostic performance than CK19, and the combined molecular detection of both genes improved diagnostic accuracy when compared with individual markers. Intraoperative molecular evaluation of sentinel lymph nodes using mammaglobin and CK19 is comparable to conventional histopathological assessment combined with immunohistochemistry. The combined RT-PCR detection of both genes improves diagnostic performance and represents a clinically useful complementary tool for the detection of metastatic involvement in early breast cancer.<\/jats:p>","DOI":"10.3390\/ijms27104462","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:40:31Z","timestamp":1779115231000},"page":"4462","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intraoperative Molecular Profiling of Sentinel Lymph Nodes: Diagnostic Value of Mammaglobin and CK19 in Early Breast Cancer"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8636-1629","authenticated-orcid":false,"given":"Diana Carolina","family":"Zambrano","sequence":"first","affiliation":[{"name":"Center for Basic Sciences, Escuela Nacional del Deporte, Cali 760001, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s Jenuer","family":"Matta","sequence":"additional","affiliation":[{"name":"Center for Basic Sciences, Escuela Nacional del Deporte, Cali 760001, Colombia"},{"name":"Faculty of Basic Sciences, Universidad Santiago de Cali, Cali 760001, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Luisa","family":"Maestro de las Casas","sequence":"additional","affiliation":[{"name":"San Carlos Clinical Hospital, 28001 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz Fernanda","family":"Sua","sequence":"additional","affiliation":[{"name":"Valle del Lili Foundation University Hospital, Cali 760001, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,16]]},"reference":[{"key":"ref_1","first-page":"394","article-title":"Global Cancer Statistics 2018: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. 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