{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:22:34Z","timestamp":1785424954387,"version":"3.56.0"},"reference-count":63,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["ORF-2026-1511"],"award-info":[{"award-number":["ORF-2026-1511"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]},{"award":["ORF-2026-1511"],"award-info":[{"award-number":["ORF-2026-1511"]}],"id":[{"id":"https:\/\/ror.org\/02f81g417","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Peucephyllum schottii is an aromatic desert plant of the family Asteraceae, which has little scientific research regarding its phytochemical composition and pharmacological properties. This study aims to evaluate in detail the chemical composition and antioxidant, antibacterial, antidiabetic, and cytotoxic activities of the ethanol extract of P. schottii leaves. The chemical composition of the plant extract was analyzed by GC-MS. Total phenolic (TPC) and flavonoid (TFC) contents of the plant were calculated. An antioxidant assay of the plant material was performed by using the DPPH and ABTS tests. The antibacterial activities of P. schottii plant material against six pathogenic bacteria were studied by using the agar diffusion and MIC\/MBC techniques. Colorimetric analysis, for its part, enabled the assessment of its antihyperglycemic activities (\u03b1-amylase and \u03b1-glucosidase) and its cytotoxic activities (in MCF-7 and HepG2 cells). The expressions of apoptotic proteins (caspases, Bcl2, and Bax), were analyzed by RT-PCR. The GC-MS findings showed the presence of complex phytoconstituents of P. schottii in the form of linoleic acid (19.48%), hexadecanoic acid (15.01%), and vitamin E (12.15%). There is high TPC (118.18 mg of GAE\/g) and TFC (75.56 mg of QE\/g) in P. schottii plant material. The plant showed significant antioxidant (\u2248105 \u03bcg\/mL IC50 in DPPH and \u224880 \u03bcg\/mL IC50 in ABTS) and broad-spectrum antibacterial activities, mostly against E. coli (MIC = 4.68 \u03bcg\/mL), as well as antihyperglycemic activities against \u03b1-amylase (IC50 = 334 \u03bcg\/mL) and \u03b1-glucosidase (IC50 = 196 \u03bcg\/mL) enzymes. The plant material showed cytotoxic effects in MCF-7 and HepG2 cells in a concentration-dependent manner (IC50 = 78 \u00b1 1.13 \u03bcg\/mL and 68.23 \u00b1 2.41 \u03bcg\/mL, respectively). These findings point to P. schottii leaf extract\u2019s potential as a natural antioxidant, antibacterial, antidiabetic, and chemopreventive agent.<\/jats:p>","DOI":"10.3390\/ijms27104497","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:40:31Z","timestamp":1779115231000},"page":"4497","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Chemical Composition, Anticancer, Antioxidant, Antibacterial, and Antidiabetic Activities of Peucephyllum schottii"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6970-5207","authenticated-orcid":false,"given":"Ibrahim M.","family":"Aziz","sequence":"first","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5632-6750","authenticated-orcid":false,"given":"Mohamed A.","family":"Farrag","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noura S.","family":"Aldosari","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najat A. Y.","family":"Marraiki","sequence":"additional","affiliation":[{"name":"Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rolnik, A., and Olas, B. (2021). The plants of the Asteraceae family as agents in the protection of human health. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22063009"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Darqui, F.S., Radonic, L.M., Beracochea, V.C., Hopp, H.E., and L\u00f3pez Bilbao, M. (2021). Peculiarities of the transformation of Asteraceae family species: The cases of sunflower and lettuce. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.767459"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Moujir, L., Callies, O., Sousa, P.M., Sharopov, F., and Seca, A.M. (2020). 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