{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T01:19:57Z","timestamp":1783127997695,"version":"3.54.6"},"reference-count":67,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The study compares the effects of the HSP60-derived mutated peptide (CIGB-258) and its wild-type peptide (E18-3) on preventing carboxymethyllysine (CML)- and ethanol (Et-OH)-induced hemorrhagic events and acute toxicity in zebrafish. The results suggest a 67% survivability and swimming recovery in CIGB-258-treated zebrafish compared to only 20% in the CML+Et-OH-treated group. No effect of E18-3 was noticed on CML+Et-OH-impaired zebrafish survivability and swimming ability. Similarly, no effect of E18-3 was noticed on the CML+Et-OH-disturbed blood oxidative and antioxidant variables. In contrast, CIGB-258 showed a notable 35% lower rate of oxidized contents, and 2.0-fold and 1.2-fold higher paraoxonase (PON) and ferric ion reduction activity (FRA), respectively, than in the E18-3 group. Also, the CML+Et-OH-induced dyslipidemia was substantially prevented by the CIGB-258, whereas no protective effect of E18-3 was noticed. Similarly, the CML+Et-OH-triggered hepatic inflammation, steatosis, kidney damage, severe gastrointestinal bleeding, and intestinal fibrosis were successfully mitigated by co-treatment with CIGB-258. Surface plasmon resonance analysis revealed a substantial binding affinity of CIGB-258 for HDL2 and HDL3, characterized by association rate constants (Ka) of 14.78 and 6.20 \u03bcM\u22121s\u22121, dissociation rate constants (Kd) of 0.35 s\u22121 and 0.22 s\u22121, and equilibrium dissociation constants (KD) of 0.024 and 0.035 \u03bcM, respectively. In conclusion, CIGB-258 exerted a substantial impact on CML+Et-OH-triggered adverse events, with high affinity for HDL, whereas E18-3 exposure remained unaffected and failed to produce any beneficial effects.<\/jats:p>","DOI":"10.3390\/ijms27104516","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:35:43Z","timestamp":1779176143000},"page":"4516","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of Anti-Acute Phase Effect of CIGB-258 and Its Wild-Type Peptide (E18-3) in a Hyperinflammatory and Acute Bleeding Model of Zebrafish: A Surface Plasmon Resonance Study to Compare Binding Affinity with High-Density Lipoproteins"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1198-4140","authenticated-orcid":false,"given":"Kyung-Hyun","family":"Cho","sequence":"first","affiliation":[{"name":"Raydel HDL Research Institute, Medical Innovation Complex, Daegu 41061, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunki","family":"Lee","sequence":"additional","affiliation":[{"name":"Raydel HDL Research Institute, Medical Innovation Complex, Daegu 41061, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Hyuk","family":"Lee","sequence":"additional","affiliation":[{"name":"Raydel HDL Research Institute, Medical Innovation Complex, Daegu 41061, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashutosh","family":"Bahuguna","sequence":"additional","affiliation":[{"name":"Raydel HDL Research Institute, Medical Innovation Complex, Daegu 41061, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung","family":"Hee Baek","sequence":"additional","affiliation":[{"name":"Raydel HDL Research Institute, Medical Innovation Complex, Daegu 41061, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda del Carmen","family":"Dom\u00ednguez-Horta","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31, e\/158 y 190, Playa, Havana 10600, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Mart\u00ednez-Donato","sequence":"additional","affiliation":[{"name":"Center for Genetic Engineering and Biotechnology, Ave 31, e\/158 y 190, Playa, Havana 10600, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"225","DOI":"10.15616\/BSL.2025.31.3.225","article-title":"Current and emerging biomarkers for sepsis: Diagnostic and prognostic tools","volume":"31","author":"Shin","year":"2025","journal-title":"Biomed. 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