{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:24:55Z","timestamp":1790886295888,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Black mustard (Brassica nigra L.) seeds are a rich source of bioactive phytochemicals; however, their antioxidant, antibacterial, and anti-inflammatory potential has not been comprehensively explored. Therefore, this study aimed to assess antioxidant, antibacterial, and anti-inflammatory effects of black mustard seed extracts obtained from Soxhlet extraction with hexane (HE) and ethanol (EE), and ultrasonic-assisted ethanolic (UE) extraction. HPLC analysis confirmed the presence of sinapic acid in all extracts. Phytochemical profiling revealed that the EE was enriched in phenolic compounds, while the UE exhibited a higher flavonoid content. Accordingly, both EE and UE demonstrated strong antioxidant activities, including radical scavenging capacity, reducing power, and inhibition of lipid peroxidation. All extracts demonstrated antibacterial activity against Staphylococcus epidermidis. The anti-inflammatory potential of extracts was supported by the inhibition of lipoxygenase and protease. The UE showed the strongest lipoxygenase inhibition, while the EE and UE exhibited comparable protease inhibitory effect. Regarding RAW264.7 cells, the extracts were non-cytotoxic and reduced the expression of IL-6 and IL-31. Molecular docking analysis suggested that sinapic acid contributes to the anti-inflammatory activity through interactions with key inflammatory targets. Overall, the EE and UE demonstrated multitarget antioxidant, antibacterial, and anti-inflammatory activities, supporting their potential application in functional and dermatological formulations for inflammation management.<\/jats:p>","DOI":"10.3390\/ijms27104603","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T15:45:34Z","timestamp":1779291934000},"page":"4603","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Brassica nigra L. Seed Extracts as a Promising Source of Antioxidant and Anti-Inflammatory Agents"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8950-8406","authenticated-orcid":false,"given":"Nichcha","family":"Nitthikan","sequence":"first","affiliation":[{"name":"Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand"},{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1493-1848","authenticated-orcid":false,"given":"Siripat","family":"Chaichit","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3014-9477","authenticated-orcid":false,"given":"Kanittapon","family":"Supadej","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, University of Phayao, Phayao 56000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4067-5799","authenticated-orcid":false,"given":"Jintana","family":"Junlatat","sequence":"additional","affiliation":[{"name":"Faculty of Thai Traditional and Alternative Medicine, Ubon Ratchathani Rajabhat University, Ubon Ratchathani 34000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4280-1079","authenticated-orcid":false,"given":"Kanokwan","family":"Kiattisin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s44187-025-00635-9","article-title":"Nutritional properties and potential therapeutic uses of Brassica nigra","volume":"5","author":"Alqaraawi","year":"2025","journal-title":"Discov. Food"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Rahman, M., Khatun, A., Liu, L., and Barkla, B.J. (2018). Brassicaceae Mustards: Traditional and Agronomic Uses in Australia and New Zealand. Molecules, 23.","DOI":"10.3390\/molecules23010231"},{"key":"ref_3","unstructured":"Duke, J. (2012). Handbook of Legumes of World Economic Importance, Springer."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lietzow, J. (2021). Biologically Active Compounds in Mustard Seeds: A Toxicological Perspective. Foods, 10.","DOI":"10.3390\/foods10092089"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3571614","DOI":"10.1155\/2016\/3571614","article-title":"Sinapic Acid and Its Derivatives as Medicine in Oxidative Stress-Induced Diseases and Aging","volume":"2016","author":"Chen","year":"2016","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4203","DOI":"10.37506\/ijfmt.v15i2.15032","article-title":"Toxicity of Cadmium Chloride on White Rats Liver and the Protective Role of Brassica nigra Seed Extract","volume":"15","author":"Asaad","year":"2021","journal-title":"Indian J. Forensic Med. Toxicol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"117","DOI":"10.2174\/2215083810666230330161740","article-title":"Phyto-pharmacology of Most Common Indian Culinary Spices and their Potential in Developing New Pharmaceutical Therapies","volume":"10","author":"Maqbool","year":"2024","journal-title":"Curr. Tradit. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1089\/ars.2012.5149","article-title":"Reactive oxygen species in inflammation and tissue injury","volume":"20","author":"Mittal","year":"2014","journal-title":"Antioxid. Redox Signal."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.cell.2006.02.015","article-title":"Pathogen recognition and innate immunity","volume":"124","author":"Akira","year":"2006","journal-title":"Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"113024","DOI":"10.1016\/j.celrep.2023.113024","article-title":"Staphylococcus epidermidis activates keratinocyte cytokine expression and promotes skin inflammation through the production of phenol-soluble modulins","volume":"42","author":"Williams","year":"2023","journal-title":"Cell Rep."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Rahman, M., Khatun, A., Liu, L., and Barkla, B.J. (2024). Brassicaceae Mustards: Phytochemical Constituents, Pharmacological Effects, and Mechanisms of Action against Human Disease. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25169039"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1007\/s43994-023-00031-y","article-title":"Impact of different solvents on extraction yield, phenolic composition, in vitro antioxidant and antibacterial activities of deseeded Opuntia stricta fruit","volume":"9","year":"2023","journal-title":"J. Umm Al-Qura Univ. Appl. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/j.tifs.2021.09.006","article-title":"Ultrasonication-A green technology extraction technique for spices: A review","volume":"116","author":"Rao","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1007\/BF02671339","article-title":"Phenolic acids in rapeseed and mustard","volume":"60","author":"Kozlowska","year":"1983","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Chadni, M., Flourat, A.L., Reungoat, V., Mouterde, L.M.M., Allais, F., and Ioannou, I. (2021). Selective Extraction of Sinapic Acid Derivatives from Mustard Seed Meal by Acting on pH: Toward a High Antioxidant Activity Rich Extract. Molecules, 26.","DOI":"10.3390\/molecules26010212"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Meneguetti, B.T., Machado, L.D.S., Oshiro, K.G.N., Nogueira, M.L., Carvalho, C.M.E., and Franco, O.L. (2017). Antimicrobial peptides from fruits and their potential use as biotechnological tools\u2014A review and outlook. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.02136"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1007\/s00217-012-1669-z","article-title":"Sinapic Acid Derivatives in Defatted Oriental Mustard (Brassica juncea L.) Seed Meal Extracts Using UHPLC-DAD-ESI-MS N and Identification of Compounds with Antibacterial Activity","volume":"234","author":"Engels","year":"2012","journal-title":"Eur. Food Res. Technol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1186\/s12967-023-04777-1","article-title":"Phenylpropanoid-enriched broccoli seedling extract can reduce inflammatory markers and pain behavior","volume":"21","author":"Gurgul","year":"2023","journal-title":"J. Transl. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.1007\/s00204-025-03997-2","article-title":"Antioxidants: A comprehensive review","volume":"99","author":"Gulcin","year":"2025","journal-title":"Arch. Toxicol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.indcrop.2019.04.059","article-title":"Biologically active compounds from white and black mustard grains: An optimization study for recovery and identification of phenolic antioxidants","volume":"135","author":"Rasera","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.jams.2012.03.003","article-title":"Evaluation of the antioxidant capacity and phenolic content of three Thymus species","volume":"5","author":"Nickavar","year":"2012","journal-title":"J. Acupunct. Meridian Stud."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/j.freeradbiomed.2010.09.006","article-title":"Oxidative stress, inflammation, and cancer: How are they linked?","volume":"49","author":"Reuter","year":"2010","journal-title":"Free Radic. Biol. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1038\/s41579-022-00780-3","article-title":"Staphylococcus epidermidis and its dual lifestyle in skin health and infection","volume":"21","author":"Severn","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3153\/FH20026","article-title":"Antibacterial and Antioxidant Activity of Some Seeds Used as Food","volume":"6","author":"Semerci","year":"2020","journal-title":"Food Health"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.ejmech.2013.11.030","article-title":"Inhibition of LOX by flavonoids: A structure-activity relationship study","volume":"72","author":"Ribeiro","year":"2014","journal-title":"Eur. J. Med. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.redox.2015.08.006","article-title":"The role of lipoxygenases in pathophysiology; new insights and future perspectives","volume":"6","author":"Mashima","year":"2015","journal-title":"Redox Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.biopha.2016.12.021","article-title":"Role of protease and protease inhibitors in cancer pathogenesis and treatment","volume":"86","author":"Eatemadi","year":"2017","journal-title":"Biomed. Pharmacother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1007\/s13596-016-0247-5","article-title":"Preliminary study on thrombolytic property of thirty six different extracts of eight Bangladeshi medicinal plants with folkloric relevance","volume":"16","author":"Khatun","year":"2016","journal-title":"Orient. Pharm. Exp. Med."},{"key":"ref_29","unstructured":"(2009). Biological Evaluation of Medical Devices\u2014Part 5: Tests for In Vitro Cytotoxicity (Standard No. ISO 10993-5)."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1038\/ni1084","article-title":"Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice","volume":"5","author":"Dillon","year":"2004","journal-title":"Nat. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6639","DOI":"10.1038\/s41598-018-25094-4","article-title":"IL-31 is crucial for induction of pruritus, but not inflammation, in contact hypersensitivity","volume":"8","author":"Takamori","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.intimp.2017.01.032","article-title":"Sinigrin inhibits production of inflammatory mediators by suppressing NF-\u03baB\/MAPK pathways or NLRP3 inflammasome activation in macrophages","volume":"45","author":"Lee","year":"2017","journal-title":"Int. Immunopharmacol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"276","DOI":"10.4062\/biomolther.2022.092","article-title":"Sinapic Acid Attenuates the Neuroinflammatory Response by Targeting AKT and MAPK in LPS-Activated Microglial Models","volume":"31","author":"Huang","year":"2023","journal-title":"Biomol. Ther."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"D\u2019Arcangelo, S., Di Fermo, P., Diban, F., Ferrone, V., D\u2019Ercole, S., Di Giulio, M., and Di Lodovico, S. (2024). Staphylococcus aureus\/Staphylococcus epidermidis from skin microbiota are balanced by Pomegranate peel extract: An eco-sustainable approach. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0308211"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1016\/j.jaci.2023.07.010","article-title":"The role of Janus kinase signaling in the pathology of atopic dermatitis","volume":"152","author":"Irvine","year":"2023","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s13765-024-00873-y","article-title":"Anti-inflammatory effects of TP1 in LPS-induced Raw264.7 macrophages","volume":"67","author":"Kim","year":"2024","journal-title":"Appl. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1110\/ps.035436.108","article-title":"Unconventional serine proteases: Variations on the catalytic Ser\/His\/Asp triad configuration","volume":"17","author":"Ekici","year":"2008","journal-title":"Protein Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1111\/1750-3841.15861","article-title":"Air frying pretreatment and the recovery of lipophilic sinapates from the oil fraction of mustard samples","volume":"86","author":"Fadairo","year":"2021","journal-title":"J. Food Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.indcrop.2018.05.001","article-title":"Optimization and kinetic modeling of oil extraction from white mustard (Sinapis alba L.) seeds","volume":"121","year":"2018","journal-title":"Ind. Crops Prod."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s11746-009-1486-0","article-title":"Determination of Sinapic Acid Derivatives in Canola Extracts Using High-Performance Liquid Chromatography","volume":"87","author":"Khattab","year":"2010","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"123","DOI":"10.5530\/pj.2018.1.22","article-title":"Phytochemical screening, total flavonoid and total phenolic content and antioxidant activity of different parts of Caesalpinia bonduc (L.) Roxb","volume":"10","author":"Sembiring","year":"2018","journal-title":"Pharmacogn. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"7865024","DOI":"10.1155\/2018\/7865024","article-title":"Improvement of stability and transdermal delivery of bioactive compounds in green robusta coffee beans extract loaded nanostructured lipid carriers","volume":"2018","author":"Nitthikan","year":"2018","journal-title":"J. Nanotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Aree, T., Chaichit, S., Junlatat, J., Kiattisin, K., and Intharuksa, A. (2025). Bridging Phytochemistry and Cosmetic Science: Molecular Insights into the Cosmeceutical Promise of Crotalaria juncea L.. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26167716"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Nitthikan, N., Preedalikit, W., Supadej, K., Chaichit, S., Leelapornpisid, P., and Kiattisin, K. (2024). Exploring the Wound Healing Potential of a Cuscuta chinensis Extract-Loaded Nanoemulsion-Based Gel. Pharmaceutics, 16.","DOI":"10.3390\/pharmaceutics16050573"},{"key":"ref_45","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2024). Antimicrobial Disk Susceptibility Tests, CLSI."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Preedalikit, W., Chittasupho, C., Leelapornpisid, P., Duangnin, N., and Kiattisin, K. (2024). Potential of Coffee Cherry Pulp Extract against Polycyclic Aromatic Hydrocarbons in Air Pollution Induced Inflammation and Oxidative Stress for Topical Applications. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25179416"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Nitthikan, N., Leelapornpisid, P., Naksuriya, O., Intasai, N., and Kiattisin, K. (2022). Potential and Alternative Bioactive Compounds from Brown Agaricus bisporus Mushroom Extracts for Xerosis Treatment. Sci. Pharm., 90.","DOI":"10.3390\/scipharm90040059"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.jep.2009.04.061","article-title":"Anti-inflammatory effect of Streblus asper leaf extract in rats and its modulation on inflammation-associated genes expression in RAW 264.7 macrophage cells","volume":"124","author":"Sripanidkulchai","year":"2009","journal-title":"J. Ethnopharmacol."},{"key":"ref_49","unstructured":"Frisch, M.J., Trucks, G.W., Schlegel, H.B., Scuseria, G.E., Robb, M.A., Cheeseman, J.R., Scalmani, G., Barone, V., Petersson, G.A., and Nakatsuji, H. (2016). Gaussian 16 Rev. B.01, Gaussian Inc."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility","volume":"30","author":"Morris","year":"2009","journal-title":"J. Comput. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"ref_52","unstructured":"Schr\u00f6dinger, LLC (2015). PyMOL Molecular Graphics System, Schr\u00f6dinger, LLC. version 1.8."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/10\/4603\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T04:16:48Z","timestamp":1779509808000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/10\/4603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":52,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["ijms27104603"],"URL":"https:\/\/doi.org\/10.3390\/ijms27104603","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]}}}