{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:02:35Z","timestamp":1785402155673,"version":"3.56.0"},"reference-count":38,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional para la Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"crossref","award":["PICTA 2021 0125"],"award-info":[{"award-number":["PICTA 2021 0125"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional para la Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"crossref","award":["Pr\u00e9stamo BID-PICTO-2021-UTCH-00005"],"award-info":[{"award-number":["Pr\u00e9stamo BID-PICTO-2021-UTCH-00005"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"crossref"}]},{"award":["PICTA 2021 0125"],"award-info":[{"award-number":["PICTA 2021 0125"]}],"id":[{"id":"https:\/\/ror.org\/03stxzb56","id-type":"ROR","asserted-by":"publisher"}]},{"award":["Pr\u00e9stamo BID-PICTO-2021-UTCH-00005"],"award-info":[{"award-number":["Pr\u00e9stamo BID-PICTO-2021-UTCH-00005"]}],"id":[{"id":"https:\/\/ror.org\/03stxzb56","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"crossref","award":["PIP 2022\u20132024 No 112202101 00799CO"],"award-info":[{"award-number":["PIP 2022\u20132024 No 112202101 00799CO"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]},{"award":["PIP 2022\u20132024 No 112202101 00799CO"],"award-info":[{"award-number":["PIP 2022\u20132024 No 112202101 00799CO"]}],"id":[{"id":"https:\/\/ror.org\/03cqe8w59","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Ministerio de Producci\u00f3n, Ciencia e Innovaci\u00f3n Tecnol\u00f3gica, Subsecretar\u00eda de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","award":["FITBA 2024 185B"],"award-info":[{"award-number":["FITBA 2024 185B"]}]},{"name":"Universidad Nacional Arturo Jauretche","award":["UNAJ Investiga 2023-Modalidad 1-Tipo A 80020200300012UJ"],"award-info":[{"award-number":["UNAJ Investiga 2023-Modalidad 1-Tipo A 80020200300012UJ"]}]},{"award":["UNAJ Investiga 2023-Modalidad 1-Tipo A 80020200300012UJ"],"award-info":[{"award-number":["UNAJ Investiga 2023-Modalidad 1-Tipo A 80020200300012UJ"]}],"id":[{"id":"https:\/\/ror.org\/00ep21g81","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic wound management remains a significant clinical challenge, requiring adaptive therapeutic approaches to achieve wound closure that nonetheless frequently prove fruitless. Balancing the initial pro-inflammatory response with debris removal and tissue rebuilding remains elusive in most cases, leading to pain, drastic quality-of-life deterioration, and, eventually, amputation. Meanwhile, patient adherence is an overarching theme. Furthermore, non-surgical alternatives that effectively promote tissue rebuilding are essential for patients seeking to avoid further invasive procedures. We report a patient with a recalcitrant ulcer managed using human amniotic membrane dressing (hAM-pe) and a bovine collagen matrix (BCM) in spatially distinct areas as an intra-patient control. Methodology included clinical monitoring and ad hoc molecular and histological analyses to assess inflammatory markers and tissue architecture. Following 59 days of observation, the superior evolution of the hAM-pe-treated zone led to the clinical decision to extend hAM-pe treatment over the adjacent BCM area, resulting in total wound closure. The hAM-pe-treated site demonstrated accelerated closure and clinical resolution of inflammation without the presence of a granulomatous response. Molecular analysis revealed downregulated pro-inflammatory mediators (IL-1\u03b2, TNF-\u03b1, CXCL-10) and upregulated markers associated with angiogenesis (VEGF, CD34) and tissue repair (Arginase-1). In this case, the non-surgical hAM-pe treatment was associated with a favorable healing trajectory, characterized by superior inflammation resolution and enhanced tissue organization (collagen type I\/III maturation). While these descriptive findings suggest the potential advantages of amniotic membrane dressings in promoting advanced tissue repair, they remain limited to this individual observation. Further research in larger cohorts is required to validate these mechanisms.<\/jats:p>","DOI":"10.3390\/ijms27114655","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T09:17:09Z","timestamp":1779441429000},"page":"4655","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Human Amniotic Membrane Dressing as a Non-Surgical Alternative for Extensive Chronic Ulcers: A Comparative Case Study"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1787-3898","authenticated-orcid":false,"given":"Mar\u00eda Ximena","family":"Guerbi","sequence":"first","affiliation":[{"name":"Centro de Medicina Traslacional, Hospital El Cruce (CEMET-HEC), Florencio Varela 1888, Argentina"},{"name":"Comisi\u00f3n de Investigaciones Cient\u00edficas de la Provincia de Buenos Aires (CIC-PBA), La Plata 1900, Argentina"},{"name":"Instituto de Ciencias de la Salud, Universidad Nacional Arturo Jauretche, Florencio Varela 1888, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimena Mar\u00eda del Pilar","family":"Rodrigo","sequence":"additional","affiliation":[{"name":"Centro de Educaci\u00f3n M\u00e9dica e Investigaciones Cl\u00ednicas \u201cNorberto Quirno\u201d (CEMIC), Buenos Aires 1425, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas Fabi\u00e1n","family":"Rotela","sequence":"additional","affiliation":[{"name":"Centro de Medicina Traslacional, Hospital El Cruce (CEMET-HEC), Florencio Varela 1888, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8255-7974","authenticated-orcid":false,"given":"Roc\u00edo Antonella","family":"Comito","sequence":"additional","affiliation":[{"name":"Centro de Medicina Traslacional, Hospital El Cruce (CEMET-HEC), Florencio Varela 1888, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Vogel","sequence":"additional","affiliation":[{"name":"Servicio de Anatom\u00eda Patol\u00f3gica, Hospital El Cruce (HEC), Florencio Varela 1888, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique Leo","family":"Portiansky","sequence":"additional","affiliation":[{"name":"Laboratorio de Im\u00e1genes (CONICET-UNLP), La Plata 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1577-7288","authenticated-orcid":false,"given":"Alejandro","family":"Berra","sequence":"additional","affiliation":[{"name":"Centro de Medicina Traslacional, Hospital El Cruce (CEMET-HEC), Florencio Varela 1888, Argentina"},{"name":"Banco de Tejidos AMNIOS-BMA, San Mart\u00edn 1650, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires 2290, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3403-0462","authenticated-orcid":false,"given":"Griselda Noem\u00ed","family":"Moreno","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias de la Salud, Universidad Nacional Arturo Jauretche, Florencio Varela 1888, Argentina"},{"name":"Instituto de Estudios Inmunol\u00f3gicos y Fisiopatol\u00f3gicos (IIFP-CONICET), La Plata 1900, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4113-3558","authenticated-orcid":false,"given":"Flavia Mariana","family":"Michelini","sequence":"additional","affiliation":[{"name":"Centro de Medicina Traslacional, Hospital El Cruce (CEMET-HEC), Florencio Varela 1888, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Buenos Aires 2290, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1067\/msg.2001.111167","article-title":"Wound healing: Biologic features and approaches to maximize healing trajectories","volume":"38","author":"Robson","year":"2001","journal-title":"Curr. 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