{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T13:05:51Z","timestamp":1779455151739,"version":"3.53.1"},"reference-count":40,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dairy Science and Technology Innovation Fund of China Dairy Industry Association","award":["CDIAKCJJ-TP-2024-007"],"award-info":[{"award-number":["CDIAKCJJ-TP-2024-007"]}]},{"name":"National Science and Technology Major Project of China","award":["2019YFC1605002"],"award-info":[{"award-number":["2019YFC1605002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Although mango is not classified among the nine major allergenic foods reported by the Food and Drug Administration (FDA), the increasing global and domestic consumption of mango has been accompanied by a growing number of reported cases of mango allergy. Currently, reports on cross-reactive allergens and cross-reactive linear epitopes in mango are limited. This study employed BLASTp (version 2.11.0+) to predict potential allergens that may cross-react with mango protein allergens and other food protein allergens. Subsequently, cross-reactive allergens were identified using sera from mango-allergic patients. Furthermore, similar sequences of the identified cross-reactive allergens were predicted by BLAST. These similar sequences were then synthesized by the solid-phase peptide synthesis method. Finally, the cross-reactive linear epitopes were determined by assessing their IgE-binding capacity using serum IgE from the same patient cohort. The results demonstrated that the sera from mango-allergic patients exhibited IgE-binding cross-reactivity with those from peanut, wheat, cashew, pistachio, and hazelnut, particularly with IgE-binding cross-reactivity to wheat and hazelnut, which has not been previously reported. The following novel cross-reactive linear epitopes were identified: the AA80\u201388 sequence of mango chitinase with the AA37\u201345 sequence of wheat Tri a 27 and the AA15\u201322 sequence of mango profilin with the AA65\u201372 sequence of pistachio Pis v 1. Furthermore, multiple cross-reactive epitopes were mapped between mango profilin and peanut Ara h 5, corresponding to the sequences AA31\u201351\/AA31\u201350, AA50\u201365\/AA52\u201365, AA76\u201396\/AA76\u201396, and AA103\u2013117\/AA104\u2013117, respectively.<\/jats:p>","DOI":"10.3390\/ijms27114670","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T12:14:52Z","timestamp":1779452092000},"page":"4670","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Mango Cross-Reactive Allergens and Cross-Reactive Linear Epitopes Using Serum from Patients with Mango Allergy"],"prefix":"10.3390","volume":"27","author":[{"given":"Wenxuan","family":"Zhao","sequence":"first","affiliation":[{"name":"Beijing Engineering and Technology Research Center of Food Additives, College of Food and Health, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglei","family":"Guo","sequence":"additional","affiliation":[{"name":"Beijing Engineering and Technology Research Center of Food Additives, College of Food and Health, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1473-2440","authenticated-orcid":false,"given":"Yanjun","family":"Cong","sequence":"additional","affiliation":[{"name":"Beijing Engineering and Technology Research Center of Food Additives, College of Food and Health, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e160","DOI":"10.1111\/jdv.14656","article-title":"A severe case of mango dermatitis","volume":"32","author":"Miyazawa","year":"2018","journal-title":"J. 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