{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:39:33Z","timestamp":1785487173309,"version":"3.56.0"},"reference-count":103,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["KPOD.07.07-IW.07-0028\/24"],"award-info":[{"award-number":["KPOD.07.07-IW.07-0028\/24"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"award":["KPOD.07.07-IW.07-0028\/24"],"award-info":[{"award-number":["KPOD.07.07-IW.07-0028\/24"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Abdominal aortic aneurysm (AAA) is a chronic vascular disease characterized by localized dilatation of the abdominal aorta. This condition is frequently underdiagnosed and carries a high mortality rate (65\u201385%) due to aneurysm rupture. Although numerous candidate biomarkers have been identified for AAA, none have been successfully implemented in clinical diagnostic procedures for AAA screening. This highlights the critical need for the discovery and validation of reliable biomarkers to improve early detection and risk stratification in AAA. Therefore, in our study, we aimed to identify small AAA (sAAA) biomarker candidates among the key cytokines and receptors of IL-1, IL-6, IL-8, IL-10, and IL-17 families on gene expression and plasma protein levels. Comparative analysis was conducted between a group of 100 men with sAAA (&lt;54 mm in diameter) and a group of 100 men without AAA. Expression profiles of the analyzed cytokines and their receptors were obtained in peripheral blood mononuclear cells using real-time PCR, while plasma levels of selected encoded proteins were determined using ELISA. The mean expression of IL10RA, IL17RA, CXCL8, and IL1B, as well as the plasma levels of IL-17A, were significantly different between the sAAA and Control groups, with CXCL8 and IL1B exhibiting a strong mutual correlation. The diagnostic model incorporating these biomarker candidates and D-dimer levels showed a fair classification performance (ROC-AUC = 0.756), with a sensitivity and specificity of approximately 0.7. The selected biomarker candidates were functionally associated with fibroblast activation, neutrophil chemotaxis, T-helper cell function, and cell adhesion and proliferation. Cytokines selected as biomarker candidates represent a promising field for further studies on the identification of diagnostic targets for the early detection of AAA.<\/jats:p>","DOI":"10.3390\/ijms27114863","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:57:43Z","timestamp":1779962263000},"page":"4863","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cytokine-Family Biomarker Candidates for Small Abdominal Aortic Aneurysm Identified via Integrated mRNA and Protein Expression Profiling"],"prefix":"10.3390","volume":"27","author":[{"given":"Piotr","family":"Stabiszewski","sequence":"first","affiliation":[{"name":"Department of Vascular Surgery, St. Padre Pio Provincial Hospital in Przemy\u015bl, 18 Monte Cassino St., 37-700 Przemy\u015bl, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2254-2009","authenticated-orcid":false,"given":"Daniel","family":"Zalewski","sequence":"additional","affiliation":[{"name":"Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Przemys\u0142aw","family":"Ko\u0142odziej","sequence":"additional","affiliation":[{"name":"Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Ziaja-So\u0142tys","sequence":"additional","affiliation":[{"name":"Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"\u0141uszczak","sequence":"additional","affiliation":[{"name":"Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Szyma\u0144ska","sequence":"additional","affiliation":[{"name":"Clinical Dietetics Unit, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"Petniak","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Medical University of Lublin, 11 Radziwi\u0142\u0142owska St., 20-080 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8683-545X","authenticated-orcid":false,"given":"Jacek","family":"Bogucki","sequence":"additional","affiliation":[{"name":"Institute of Medical Sciences, The John Paul II (The Second) Catholic University of Lublin, Konstantyn\u00f3w 1F St., 20-708 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Terlecki","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery and Angiology, Medical University of Lublin, 8 Solidarno\u015bci St., 20-841 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Stawarz","sequence":"additional","affiliation":[{"name":"St. Padre Pio Provincial Hospital in Przemy\u015bl, 18 Monte Cassino St., 37-700 Przemy\u015bl, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janusz","family":"Kocki","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Medical University of Lublin, 11 Radziwi\u0142\u0142owska St., 20-080 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7406-1728","authenticated-orcid":false,"given":"Marcin","family":"Feldo","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery and Angiology, Medical University of Lublin, 8 Solidarno\u015bci St., 20-841 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Bogucka-Kocka","sequence":"additional","affiliation":[{"name":"Department of Biology and Genetics, Medical University of Lublin, 4a Chod\u017aki St., 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kessler, V., Klopf, J., Eilenberg, W., Neumayer, C., and Brostjan, C. (2022). AAA Revisited: A Comprehensive Review of Risk Factors, Management, and Hallmarks of Pathogenesis. Biomedicines, 10.","DOI":"10.3390\/biomedicines10010094"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/s41572-018-0030-7","article-title":"Abdominal Aortic Aneurysms","volume":"4","author":"Sakalihasan","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1056\/NEJMcp1401430","article-title":"Abdominal Aortic Aneurysms","volume":"371","author":"Kent","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1097\/SLA.0000000000005716","article-title":"The Global and Regional Prevalence of Abdominal Aortic Aneurysms: A Systematic Review and Modeling Analysis","volume":"277","author":"Song","year":"2023","journal-title":"Ann. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2219","DOI":"10.1001\/jama.2019.17021","article-title":"Primary Care Screening for Abdominal Aortic Aneurysm: Updated Evidence Report and Systematic Review for the US Preventive Services Task Force","volume":"322","author":"Beil","year":"2019","journal-title":"JAMA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1007\/s00772-014-1348-4","article-title":"Abdominal Aortic Aneurysm Screening Program in Poland","volume":"19","author":"Jawien","year":"2014","journal-title":"Gefasschirurgie"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"705","DOI":"10.5603\/KP.a2017.0071","article-title":"The Prevalence of Abdominal Aortic Aneurysms in the Rural\/Urban Population in Central Poland\u2014Gniewkowo Aortic Study","volume":"75","author":"Migdalski","year":"2017","journal-title":"Kardiol. Pol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5603\/AA.2019.0001","article-title":"Prevalence and Risk Factors of Abdominal Aortic Aneurysm among over 65 Years Old Population in Lublin, Poland","volume":"25","author":"Tkaczyk","year":"2019","journal-title":"Acta Angiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Li, X., Zhao, G., Zhang, J., Duan, Z., and Xin, S. (2013). Prevalence and Trends of the Abdominal Aortic Aneurysms Epidemic in General Population--a Meta-Analysis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0081260"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1002\/bjs.10200","article-title":"Population-Based Study of Ruptured Abdominal Aortic Aneurysm","volume":"103","author":"Laine","year":"2016","journal-title":"Br. J. Surg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.ejvs.2023.09.039","article-title":"Editor\u2019s Choice\u2014Systematic Review and Meta-Analysis of Contemporary Abdominal Aortic Aneurysm Growth Rates","volume":"67","author":"Prendes","year":"2024","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Huang, T., Liu, S., Huang, J., Xu, B., Bai, Y., and Wang, W. (2019). Meta-Analysis of the Growth Rates of Abdominal Aortic Aneurysm in the Chinese Population. BMC Cardiovasc. Disord., 19.","DOI":"10.1186\/s12872-019-1160-x"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Leone, N., Broda, M.A., Eiberg, J.P., and Resch, T.A. (2023). Systematic Review and Meta-Analysis of the Incidence of Rupture, Repair, and Death of Small and Large Abdominal Aortic Aneurysms under Surveillance. J. Clin. Med., 12.","DOI":"10.3390\/jcm12216837"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.ejvs.2016.10.013","article-title":"Patients with Small Abdominal Aortic Aneurysm Are at Significant Risk of Cardiovascular Events and This Risk Is Not Addressed Sufficiently","volume":"53","author":"Bath","year":"2017","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.ejvs.2023.11.002","article-title":"Editor\u2019s Choice\u2014European Society for Vascular Surgery (ESVS) 2024 Clinical Practice Guidelines on the Management of Abdominal Aorto-Iliac Artery Aneurysms\u2606","volume":"67","author":"Wanhainen","year":"2024","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_16","first-page":"CD001835","article-title":"Surgery for Small Asymptomatic Abdominal Aortic Aneurysms","volume":"7","author":"Ulug","year":"2020","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3310\/hta17410","article-title":"Systematic Review and Meta-Analysis of the Growth and Rupture Rates of Small Abdominal Aortic Aneurysms: Implications for Surveillance Intervals and Their Cost-Effectiveness","volume":"17","author":"Thompson","year":"2013","journal-title":"Health Technol. Assess."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2\u201377.e2","DOI":"10.1016\/j.jvs.2017.10.044","article-title":"The Society for Vascular Surgery Practice Guidelines on the Care of Patients with an Abdominal Aortic Aneurysm","volume":"67","author":"Chaikof","year":"2018","journal-title":"J. Vasc. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/joim.12958","article-title":"Lack of an Effective Drug Therapy for Abdominal Aortic Aneurysm","volume":"288","author":"Golledge","year":"2020","journal-title":"J. Intern. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11772719221095676","DOI":"10.1177\/11772719221095676","article-title":"Cytokines in Abdominal Aortic Aneurysm: Master Regulators With Clinical Application","volume":"17","author":"Puchenkova","year":"2022","journal-title":"Biomark. Insights"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Yuan, Z., Lu, Y., Wei, J., Wu, J., Yang, J., and Cai, Z. (2020). Abdominal Aortic Aneurysm: Roles of Inflammatory Cells. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.609161"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1038\/nrcardio.2017.52","article-title":"Monocytes and Macrophages in Abdominal Aortic Aneurysm","volume":"14","author":"Raffort","year":"2017","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"12077","DOI":"10.2147\/DDDT.S563581","article-title":"Therapeutic Targeting of Signaling Pathways in Abdominal Aortic Aneurysm: From Pathogenesis to Precision Medicine","volume":"19","author":"Shaikh","year":"2025","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2382","DOI":"10.1161\/CIRCULATIONAHA.108.802074","article-title":"Circulating Markers of Abdominal Aortic Aneurysm Presence and Progression","volume":"118","author":"Golledge","year":"2008","journal-title":"Circulation"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"925840","DOI":"10.1155\/2014\/925840","article-title":"Novel Biomarkers of Abdominal Aortic Aneurysm Disease: Identifying Gaps and Dispelling Misperceptions","volume":"2014","author":"Moris","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"19","DOI":"10.5603\/AA.2019.0004","article-title":"The Review of Selected Biomarkers of Abdominal Aortic Aneurysm","volume":"25","author":"Surma","year":"2019","journal-title":"Acta Angiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.ejvs.2008.05.009","article-title":"Potential Circulating Biomarkers for Abdominal Aortic Aneurysm Expansion and Rupture--a Systematic Review","volume":"36","author":"Urbonavicius","year":"2008","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Xu, Y., Yang, S., and Xue, G. (2023). The Role of Long Non-Coding RNA in Abdominal Aortic Aneurysm. Front. Genet., 14.","DOI":"10.3389\/fgene.2023.1153899"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Gurung, R., Choong, A.M., Woo, C.C., Foo, R., and Sorokin, V. (2020). Genetic and Epigenetic Mechanisms Underlying Vascular Smooth Muscle Cell Phenotypic Modulation in Abdominal Aortic Aneurysm. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176334"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"100399","DOI":"10.1016\/j.jvssci.2025.100399","article-title":"A Narrative Review of Recent Literature of Circulating Biomarkers of Abdominal Aortic Aneurysm","volume":"7","author":"Rhee","year":"2026","journal-title":"JVS Vasc. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"12984","DOI":"10.18632\/oncotarget.3848","article-title":"Differential Gene Expression in Human Abdominal Aortic Aneurysm and Aortic Occlusive Disease","volume":"6","author":"Biros","year":"2015","journal-title":"Oncotarget"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"8980","DOI":"10.1080\/21655979.2021.1990822","article-title":"POU Class 2 Homeobox Associating Factor 1 (POU2AF1) Participates in Abdominal Aortic Aneurysm Enlargement Based on Integrated Bioinformatics Analysis","volume":"12","author":"Meng","year":"2021","journal-title":"Bioengineered"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1007\/s00268-002-6690-0","article-title":"Enhanced Tumor Necrosis Factor-\u03b1 Expression in Small Sized Abdominal Aortic Aneurysms","volume":"27","author":"Hamano","year":"2003","journal-title":"World J. Surg."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.thromres.2009.01.016","article-title":"Haemostatic Markers in Patients with Abdominal Aortic Aneurysm and the Impact of Aneurysm Size","volume":"124","author":"Wallinder","year":"2009","journal-title":"Thromb. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/j.jvs.2016.05.093","article-title":"A Potential Contribution of Dipeptidyl Peptidase-4 by the Mediation of Monocyte Differentiation in the Development and Progression of Abdominal Aortic Aneurysms","volume":"66","author":"Lu","year":"2017","journal-title":"J. Vasc. Surg."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jvs.2010.02.279","article-title":"Markers of Inflammation in Men with Small Abdominal Aortic Aneurysm","volume":"52","author":"Parry","year":"2010","journal-title":"J. Vasc. Surg."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3011","DOI":"10.1161\/ATVBAHA.111.237537","article-title":"Proteomic Analysis of Polymorphonuclear Neutrophils Identifies Catalase as a Novel Biomarker of Abdominal Aortic Aneurysm: Potential Implication of Oxidative Stress in Abdominal Aortic Aneurysm Progression","volume":"31","author":"Lopez","year":"2011","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/j.jvs.2022.12.001","article-title":"The Predictive Value of Four Serum Biomarkers for Major Adverse Events in Patients with Small Abdominal Aortic Aneurysm","volume":"77","author":"Golledge","year":"2023","journal-title":"J. Vasc. Surg."},{"key":"ref_39","unstructured":"(2025). The UniProt Consortium UniProt: The Universal Protein Knowledgebase in 2025. Nucleic Acids Res., 53, D609\u2013D617."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1109\/LSP.2014.2337313","article-title":"Fast Implementation of DeLong\u2019s Algorithm for Comparing the Areas Under Correlated Receiver Operating Characteristic Curves","volume":"21","author":"Sun","year":"2014","journal-title":"IEEE Signal Process. Lett."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1161\/ATVBAHA.110.206573","article-title":"Atherosclerosis and Abdominal Aortic Aneurysm: Cause, Response or Common Risk Factors?","volume":"30","author":"Golledge","year":"2010","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1161\/01.CIR.102.10.1186","article-title":"Arterial Remodeling. Mechanisms and Clinical Implications","volume":"102","author":"Ward","year":"2000","journal-title":"Circulation"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/pro.4218","article-title":"PANTHER: Making Genome-Scale Phylogenetics Accessible to All","volume":"31","author":"Thomas","year":"2022","journal-title":"Protein Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Ciborowski, M., Teul, J., Martin-Ventura, J.L., Egido, J., and Barbas, C. (2012). Metabolomics with LC-QTOF-MS Permits the Prediction of Disease Stage in Aortic Abdominal Aneurysm Based on Plasma Metabolic Fingerprint. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0031982"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.atherosclerosis.2010.11.009","article-title":"Soluble TWEAK Plasma Levels Predict Expansion of Human Abdominal Aortic Aneurysms","volume":"214","author":"Lindholt","year":"2011","journal-title":"Atherosclerosis"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.clinbiochem.2009.07.025","article-title":"Plasma Concentrations of Growth Arrest Specific Protein 6 and the Soluble Form of Its Tyrosine Kinase Receptor Axl as Markers of Large Abdominal Aortic Aneurysms","volume":"43","author":"Ekman","year":"2010","journal-title":"Clin. Biochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1053\/ejvs.2000.1151","article-title":"The Plasma Level of Matrix Metalloproteinase 9 May Predict the Natural History of Small Abdominal Aortic Aneurysms. A Preliminary Study","volume":"20","author":"Lindholt","year":"2000","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1053\/ejvs.2002.1872","article-title":"Relationships between Activators and Inhibitors of Plasminogen, and the Progression of Small Abdominal Aortic Aneurysms","volume":"25","author":"Lindholt","year":"2003","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1128\/IAI.05697-11","article-title":"Interleukin-1\u03b2 Regulates CXCL8 Release and Influences Disease Outcome in Response to Streptococcus Pneumoniae, Defining Intercellular Cooperation between Pulmonary Epithelial Cells and Macrophages","volume":"80","author":"Marriott","year":"2012","journal-title":"Infect. Immun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1080\/08820139.2020.1787436","article-title":"Neutralization of TNF-\u03b1 and IL-1\u03b2 Regulates CXCL8 Production through CXCL8\/CXCR1 Axis in Macrophages during Staphylococcus Aureus Infection","volume":"50","author":"Dutta","year":"2021","journal-title":"Immunol. Investig."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"00368504251328754","DOI":"10.1177\/00368504251328754","article-title":"CXCL8 Upregulation Mediates Inflammatory Cell Infiltration and Accelerates Abdominal Aortic Aneurysm Progression","volume":"108","author":"Huang","year":"2025","journal-title":"Sci. Prog."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1161\/ATVBAHA.117.309897","article-title":"Novel Role of IL (Interleukin)-1\u03b2 in Neutrophil Extracellular Trap Formation and Abdominal Aortic Aneurysms","volume":"38","author":"Meher","year":"2018","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"S51","DOI":"10.1161\/CIRCULATIONAHA.113.006800","article-title":"Inhibition of Interleukin-1\u03b2 Decreases Aneurysm Formation and Progression in a Novel Model of Thoracic Aortic Aneurysms","volume":"130","author":"Johnston","year":"2014","journal-title":"Circulation"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.cyto.2018.03.031","article-title":"CXCL8 Hyper-Signaling in the Aortic Abdominal Aneurysm","volume":"108","author":"Kokje","year":"2018","journal-title":"Cytokine"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"108257","DOI":"10.1016\/j.pharmthera.2022.108257","article-title":"Targeting CXCR1 and CXCR2 Receptors in Cardiovascular Diseases","volume":"237","author":"Dhayni","year":"2022","journal-title":"Pharmacol. Ther."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"22","DOI":"10.33696\/immunology.5.163","article-title":"IL-1 in Abdominal Aortic Aneurysms","volume":"5","author":"Millar","year":"2023","journal-title":"J. Cell. Immunol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.cca.2020.01.007","article-title":"The Role of IL-1\u03b2 in Aortic Aneurysm","volume":"504","author":"Wenjing","year":"2020","journal-title":"Clin. Chim. Acta"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.immuni.2017.10.016","article-title":"Targeting Interleukin-1\u03b2 Protects from Aortic Aneurysms Induced by Disrupted Transforming Growth Factor \u03b2 Signaling","volume":"47","author":"Carnevale","year":"2017","journal-title":"Immunity"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Zalewski, D., Chmiel, P., Ko\u0142odziej, P., Borowski, G., Feldo, M., Kocki, J., and Bogucka-Kocka, A. (2023). Dysregulations of Key Regulators of Angiogenesis and Inflammation in Abdominal Aortic Aneurysm. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241512087"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Thanigaimani, S., Ibrahim, M., and Golledge, J. (2022). Potential of Disease-Modifying Anti-Rheumatic Drugs to Limit Abdominal Aortic Aneurysm Growth. Biomedicines, 10.","DOI":"10.3390\/biomedicines10102409"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"9034996","DOI":"10.1155\/2022\/9034996","article-title":"Bioinformatics and System Biological Approaches for the Identification of Genetic Risk Factors in the Progression of Cardiovascular Disease","volume":"2022","author":"Barua","year":"2022","journal-title":"Cardiovasc. Ther."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2230","DOI":"10.3934\/mbe.2021112","article-title":"Identification of Potential Genes Associated with Immune Cell Infiltration in Atherosclerosis","volume":"18","author":"Xia","year":"2021","journal-title":"Math. Biosci. Eng."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"245","DOI":"10.3109\/07853890.2015.1019916","article-title":"Plasma Cytokine Levels and Risks of Abdominal Aortic Aneurysms: A Population-Based Prospective Cohort Study","volume":"47","author":"Liao","year":"2015","journal-title":"Ann. Med."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"S38","DOI":"10.1161\/CIRCULATIONAHA.111.083451","article-title":"Experimental Abdominal Aortic Aneurysm Formation Is Mediated by IL-17 and Attenuated by Mesenchymal Stem Cell Treatment","volume":"126","author":"Sharma","year":"2012","journal-title":"Circulation"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.1161\/ATVBAHA.114.304435","article-title":"Inhibiting the Th17\/IL-17A-Related Inflammatory Responses with Digoxin Confers Protection against Experimental Abdominal Aortic Aneurysm","volume":"34","author":"Wei","year":"2014","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.ijcard.2020.03.072","article-title":"Experimental Abdominal Aortic Aneurysm Growth Is Inhibited by Blocking the JAK2\/STAT3 Pathway","volume":"312","author":"Xiao","year":"2020","journal-title":"Int. J. Cardiol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1161\/CIRCGENETICS.111.961235","article-title":"Frequent and Widespread Vascular Abnormalities in Human Signal Transducer and Activator of Transcription 3 Deficiency","volume":"5","author":"Chandesris","year":"2012","journal-title":"Circ. Cardiovasc. Genet."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1161\/ATVBAHA.112.300516","article-title":"Overexpression of SOCS3 in T Lymphocytes Leads to Impaired Interleukin-17 Production and Severe Aortic Aneurysm Formation in Mice--Brief Report","volume":"33","author":"Romain","year":"2013","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1002\/ame2.12572","article-title":"Animal Models for Abdominal Aortic Aneurysms: Where We Are and Where We Need to Go","volume":"8","author":"Tian","year":"2025","journal-title":"Anim. Model. Exp. Med."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1038\/s41392-023-01620-3","article-title":"The IL-17 Family in Diseases: From Bench to Bedside","volume":"8","author":"Huangfu","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"110757","DOI":"10.1016\/j.intimp.2023.110757","article-title":"IL-17A Functions and the Therapeutic Use of IL-17A and IL-17RA Targeted Antibodies for Cancer Treatment","volume":"123","author":"Song","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-Monta\u00f1o, R., Ruiz-Guti\u00e9rrez, A.d.C., Mart\u00ednez-Rodr\u00edguez, V.M.d.C., G\u00f3mez-Sandoval, J.R., Guzm\u00e1n-Flores, J.M., Becerra-Ruiz, J.S., Zamora-Perez, A.L., and Guerrero-Vel\u00e1zquez, C. (2022). Levels of IL-23\/IL-17 Axis in Plasma and Gingival Tissue of Periodontitis Patients According to the New Classification. Appl. Sci., 12.","DOI":"10.3390\/app12168051"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"\u010celakovsk\u00e1, J., \u010cerm\u00e1kov\u00e1, E., Boudkova, P., and Andr\u00fds, C. (2026). The Evaluation of the Plasma Levels of Interleukin 17A, Thymic Stromal Lymphopoietin, Interferon Gamma, Tumor Necrosis Factor-Alpha and Interleukins IL-2, IL-6, IL-23, and IL-31 in Atopic Dermatitis Patients with Dupilumab Treatment. Front. Immunol., 17.","DOI":"10.3389\/fimmu.2026.1748929"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Dhaouadi, T., Chahbi, M., Haouami, Y., Sfar, I., Abdelmoula, L., Abdallah, T.B., and Gorgi, Y. (2018). IL-17A, IL-17RC Polymorphisms and IL17 Plasma Levels in Tunisian Patients with Rheumatoid Arthritis. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194883"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e20471","DOI":"10.1016\/j.heliyon.2023.e20471","article-title":"Screening of Plasma IL-6 and IL-17 in Bangladeshi Lung Cancer Patients","volume":"9","author":"Shill","year":"2023","journal-title":"Heliyon"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.cca.2018.03.014","article-title":"Increased Interleukin-11 Levels in Thoracic Aorta and Plasma from Patients with Acute Thoracic Aortic Dissection","volume":"481","author":"Xu","year":"2018","journal-title":"Clin. Chim. Acta"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.bbrc.2022.03.051","article-title":"IL-17A Promotes Vascular Calcification in an Ex Vivo Murine Aorta Culture","volume":"604","author":"Mukai","year":"2022","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"154089","DOI":"10.1016\/j.cyto.2017.06.016","article-title":"Interleukin 17A in Atherosclerosis\u2014Regulation and Pathophysiologic Effector Function","volume":"122","author":"Nordlohne","year":"2019","journal-title":"Cytokine"},{"key":"ref_79","first-page":"1260","article-title":"Interleukin-10 Promotes Proliferation of Vascular Smooth Muscle Cells by Inhibiting Inflammation in Rabbit Abdominal Aortic Aneurysm","volume":"12","author":"Zhu","year":"2019","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1161\/ATVBAHA.117.310672","article-title":"Systemic Upregulation of IL-10 (Interleukin-10) Using a Nonimmunogenic Vector Reduces Growth and Rate of Dissecting Abdominal Aortic Aneurysm","volume":"38","author":"Adam","year":"2018","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.1161\/ATVBAHA.113.301280","article-title":"Natural Regulatory T Cells Limit Angiotensin II-Induced Aneurysm Formation and Rupture in Mice","volume":"33","author":"Wang","year":"2013","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.regpep.2012.08.014","article-title":"Arterial Stiffness and Novel Biomarkers in Patients with Abdominal Aortic Aneurysms","volume":"179","author":"Kadoglou","year":"2012","journal-title":"Regul. Pept."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Zhang, Q., Liu, H., Zhang, M., Liu, F., and Liu, T. (2023). Identification of Co-Expressed Central Genes and Transcription Factors in Atherosclerosis-Related Intracranial Aneurysm. Front. Neurol., 14.","DOI":"10.3389\/fneur.2023.1055456"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"3088","DOI":"10.1182\/bloodadvances.2017013359","article-title":"Prognostic Value of D-Dimer and Markers of Coagulation for Stratification of Abdominal Aortic Aneurysm Growth","volume":"2","author":"Sundermann","year":"2018","journal-title":"Blood Adv."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1111\/jth.13487","article-title":"Plasma D-Dimer as a Predictor of the Progression of Abdominal Aortic Aneurysm","volume":"14","author":"Vele","year":"2016","journal-title":"J. Thromb. Haemost."},{"key":"ref_86","first-page":"405","article-title":"Is Serum Matrix Metalloproteinase 9 and\/or D-Dimer Levels a Marker for Identifying Abdominal Aortic Aneurysms in Patients with Significant Coronary Atherosclerosis?","volume":"50","author":"Brie","year":"2024","journal-title":"Curr. Health Sci. J."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.ejvs.2009.05.013","article-title":"Plasma Fibrinogen and D-Dimer Concentrations Are Associated with the Presence of Abdominal Aortic Aneurysm: A Systematic Review and Meta-Analysis","volume":"38","author":"Takagi","year":"2009","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Cai, H., Pan, B., Xu, J., Liu, S., Wang, L., Wu, K., Yang, P., Huang, J., and Wang, W. (2022). D-Dimer Is a Diagnostic Biomarker of Abdominal Aortic Aneurysm in Patients With Peripheral Artery Disease. Front. Cardiovasc. Med., 9.","DOI":"10.3389\/fcvm.2022.890228"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1093\/eurheartj\/ehq171","article-title":"Evaluation of the Diagnostic and Prognostic Value of Plasma D-Dimer for Abdominal Aortic Aneurysm","volume":"32","author":"Golledge","year":"2011","journal-title":"Eur. Heart J."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Zagrapan, B., Klopf, J., Celem, N.D., Brandau, A., Rossi, P., Gordeeva, Y., Szewczyk, A.R., Liu, L., Ahmadi-Fazel, D., and Najarnia, S. (2023). Diagnostic Utility of a Combined MPO\/D-Dimer Score to Distinguish Abdominal Aortic Aneurysm from Peripheral Artery Disease. J. Clin. Med., 12.","DOI":"10.3390\/jcm12247558"},{"key":"ref_91","first-page":"1035","article-title":"How We Manage a High D-Dimer","volume":"109","author":"Franchini","year":"2024","journal-title":"Haematologica"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"201","DOI":"10.2143\/AC.64.2.2036138","article-title":"Expression or Secretion of IL-17 in the Peripheral Blood Mononuclear Cells from Patients with Dilated Cardiomyopathy","volume":"64","author":"Huang","year":"2009","journal-title":"Acta Cardiol."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Obasanmi, G., Lois, N., Armstrong, D., Hombrebueno, J.M.R., Lynch, A., Chen, M., and Xu, H. (2023). Peripheral Blood Mononuclear Cells from Patients with Type 1 Diabetes and Diabetic Retinopathy Produce Higher Levels of IL-17A, IL-10 and IL-6 and Lower Levels of IFN-\u03b3\u2014A Pilot Study. Cells, 12.","DOI":"10.3390\/cells12030467"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.cyto.2013.07.005","article-title":"Increased IL-23 and IL-17 Expression by Peripheral Blood Cells of Patients with Primary Biliary Cirrhosis","volume":"64","author":"Qian","year":"2013","journal-title":"Cytokine"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1007\/s10875-012-9701-4","article-title":"Requirements for Growth and IL-10 Expression of Highly Purified Human T Regulatory Cells","volume":"32","author":"Bonacci","year":"2012","journal-title":"J. Clin. Immunol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"110728","DOI":"10.1016\/j.celrep.2022.110728","article-title":"Human IL-10-Producing B Cells Have Diverse States That Are Induced from Multiple B Cell Subsets","volume":"39","author":"Glass","year":"2022","journal-title":"Cell Rep."},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Bogucka-Kocka, A., Zalewski, D.P., Ruszel, K.P., St\u0119pniewski, A., Ga\u0142kowski, D., Bogucki, J., Komsta, \u0141., Ko\u0142odziej, P., Zubilewicz, T., and Feldo, M. (2019). Dysregulation of MicroRNA Regulatory Network in Lower Extremities Arterial Disease. Front. Genet., 10.","DOI":"10.3389\/fgene.2019.01200"},{"key":"ref_98","unstructured":"R Core Team (2023). R: A Language and Environment for Statistical Computing, R Core Team."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"29762","DOI":"10.1038\/s41598-025-11822-0","article-title":"RQdeltaCT: An Open-Source R Package for Relative Quantification of Gene Expression Using Delta Ct Methods","volume":"15","author":"Zalewski","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Analyzing Real-Time PCR Data by the Comparative C(T) Method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Robin, X., Turck, N., Hainard, A., Tiberti, N., Lisacek, F., Sanchez, J.-C., and M\u00fcller, M. (2011). pROC: An Open-Source Package for R and S+ to Analyze and Compare ROC Curves. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-77"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v028.i05","article-title":"Building Predictive Models in R Using the Caret Package","volume":"28","author":"Kuhn","year":"2008","journal-title":"J. Stat. Softw."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/11\/4863\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:58:51Z","timestamp":1779962331000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/11\/4863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,28]]},"references-count":103,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ijms27114863"],"URL":"https:\/\/doi.org\/10.3390\/ijms27114863","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,28]]}}}