{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:27:25Z","timestamp":1785421645409,"version":"3.56.0"},"reference-count":36,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, Romania","award":["511\/2\/17.01.2022"],"award-info":[{"award-number":["511\/2\/17.01.2022"]}]},{"award":["511\/2\/17.01.2022"],"award-info":[{"award-number":["511\/2\/17.01.2022"]}],"id":[{"id":"https:\/\/ror.org\/03gwbzf29","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Obesity and periodontitis are chronic diseases that share inflammatory and metabolic pathways. The RBFOX1 gene was selected as an exploratory candidate gene because it encodes an RNA-binding splicing regulator, has been implicated in obesity-related phenotypes, and has been reported in candidate-gene analyses of periodontitis\/metabolic traits. This study investigated the association between obesity, periodontal disease severity, and RBFOX1 rs6500744 polymorphism. This case\u2013control study enrolled 106 adults: 53 with obesity (BMI \u2265 30 kg\/m2) and 53 normoweight controls. Clinical and radiographic periodontal assessments determined disease severity, complexity, staging, and grading. Genotyping of the RBFOX1 rs6500744 polymorphism (CC, CT, TT) was performed using the TaqMan\u00ae SNP Genotyping Assay and the 7500 Fast Dx Real-Time PCR system. Multivariable models adjusted for age, sex, smoking status, and clinically confirmed type 2 diabetes. Participants with obesity showed higher levels of periodontal disease than normoweight controls, indicated by greater pocket depths, attachment loss, and bone loss. Advanced stage (Stage III\/IV; p = 5.35 \u00d7 10\u22126) and Grade C periodontitis (p = 2.08 \u00d7 10\u22127) were significantly more frequent in the obese group. The rs6500744 T allele was more common among individuals with obesity (46.2% vs. 30.2%; OR 1.99, p = 0.023). Periodontal damage appeared to increase progressively from CC to CT to TT genotype, but genotype-stratified estimates, particularly for TT homozygotes, were interpreted cautiously because of the small subgroup size and multiple testing. Both obesity and the number of T alleles were associated with increased periodontal severity in adjusted statistical models. VIFs were low, residual diagnostics did not indicate major assumption violations, smoking-stratified sensitivity analyses were directionally consistent, and the obesity \u00d7 T-allele interaction was not statistically significant. Obesity is associated with more severe and extensive periodontal disease in this exploratory case\u2013control cohort; however, residual confounding from significant smoking imbalances between groups cannot be excluded. The RBFOX1 rs6500744 T allele may mark increased susceptibility to periodontal tissue destruction, but the findings do not establish causality or clinical prognostic utility and require longitudinal validation in larger, ancestry-controlled populations.<\/jats:p>","DOI":"10.3390\/ijms27114867","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:57:43Z","timestamp":1779962263000},"page":"4867","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association of the RBFOX1 rs6500744 Polymorphism with Periodontal Disease Severity in Adults with Obesity: A Case\u2013Control Study"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1019-864X","authenticated-orcid":false,"given":"Felicia Gabriela","family":"Beresescu","sequence":"first","affiliation":[{"name":"Faculty of Dental Medicine, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mure\u0219, 540142 Targu Mure\u0219, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela-Tatiana","family":"Sala","sequence":"additional","affiliation":[{"name":"2nd Department of Surgery, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mure\u0219, 540142 Targu Mure\u0219, Romania"},{"name":"2nd Clinic of Surgery, Mure\u0219 County Emergency Clinical Hospital, 540136 Targu Mure\u0219, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Ormenisan","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mure\u0219, 540142 Targu Mure\u0219, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7147-5623","authenticated-orcid":false,"given":"Andrea","family":"Bors","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mure\u0219, 540142 Targu Mure\u0219, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3998-0638","authenticated-orcid":false,"given":"Melinda","family":"Sz\u00e9kely","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mure\u0219, 540142 Targu Mure\u0219, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0176-1894","authenticated-orcid":false,"given":"Liana","family":"Beresescu","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mure\u0219, 540142 Targu Mure\u0219, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana-Stela","family":"Crisan","sequence":"additional","affiliation":[{"name":"Genetics Department, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mure\u0219, 540136 Targu Mure\u0219, Romania"},{"name":"Genetics Laboratory, Center for Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mure\u0219, 540139 Targu Mure\u0219, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"ref_1","unstructured":"(2026, February 16). 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