{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:27:17Z","timestamp":1785414437503,"version":"3.56.0"},"reference-count":73,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005916","name":"Universidad de La Frontera","doi-asserted-by":"publisher","award":["DIUFRO DI24-0029"],"award-info":[{"award-number":["DIUFRO DI24-0029"]}],"id":[{"id":"10.13039\/501100005916","id-type":"DOI","asserted-by":"publisher"}]},{"award":["DIUFRO DI24-0029"],"award-info":[{"award-number":["DIUFRO DI24-0029"]}],"id":[{"id":"https:\/\/ror.org\/04v0snf24","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Visceral adipose tissue (VAT) is a metabolically active organ that undergoes structural and functional remodeling under obesogenic conditions. Early-life stress, such as maternal separation (MS), may modulate these processes, but its depot-specific effects remain poorly characterized. This study aimed to determine whether MS modulates VAT remodeling in response to post-weaning high-fat diet (HFD) exposure in male C57BL\/6 mice. Animals underwent MS during the early postnatal period (PND2\u201316) or remained unmanipulated (UM), and were subsequently fed either a control diet (CD) or an HFD for 16 weeks (groups: UM-CD, UM-HFD, MS-CD, MS-HFD). Visceral adipose tissue was collected and analyzed at PND133. Perigonadal (PGAT), retroperitoneal (RPAT), and mesenteric (MSAT) visceral adipose tissue deposits were analyzed by histology, Picrosirius Red staining, and immunohistochemistry for leptin and UCP-1; apoptosis was assessed by TUNEL assay. HFD induced adipocyte hypertrophy and early inflammatory changes, while MS predominantly affected stromal organization. Collagen remodeling was depot-specific: PGAT showed an adaptive pattern, RPAT exhibited a significant MS\u00d7HFD interaction, and MSAT was primarily affected by MS regardless of diet. Leptin immunoreactivity increased with HFD in UM animals but was attenuated in MS mice, particularly in MSAT. UCP-1 signal was low and heterogeneous, without clear morphological browning. Apoptosis increased in MSAT under MS-HFD conditions. These findings indicate that early-life stress programs depot-specific VAT remodeling, with MSAT emerging as particularly susceptible to obesogenic challenge.<\/jats:p>","DOI":"10.3390\/ijms27115056","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:46:48Z","timestamp":1780494408000},"page":"5056","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Maternal Separation Differentially Programs Structural and Functional Remodeling of Visceral Adipose Tissue Depots in Mice Exposed to a Post-Weaning High-Fat Diet"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5347-4820","authenticated-orcid":false,"given":"Javiera","family":"Navarrete","sequence":"first","affiliation":[{"name":"Doctoral Program in Morphological Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco 4780000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4106-3548","authenticated-orcid":false,"given":"B\u00e9lgica","family":"V\u00e1squez","sequence":"additional","affiliation":[{"name":"Doctoral Program in Morphological Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco 4780000, Chile"},{"name":"Center of Excellence in Morphological and Surgical Studies, Universidad de La Frontera, Temuco 4811230, Chile"},{"name":"Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Barthelemy, J., Bogard, G., and Wolowczuk, I. (2023). Beyond Energy Balance Regulation: The Underestimated Role of Adipose Tissues in Host Defense against Pathogens. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1083191"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hemat Jouy, S., Mohan, S., Scichilone, G., Mostafa, A., and Mahmoud, A.M. (2024). Adipokines in the Crosstalk between Adipose Tissues and Other Organs: Implications in Cardiometabolic Diseases. Biomedicines, 12.","DOI":"10.3390\/biomedicines12092129"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"C375","DOI":"10.1152\/ajpcell.00379.2020","article-title":"Adipose Tissue Inflammation and Metabolic Dysfunction in Obesity","volume":"320","author":"Kawai","year":"2021","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Kolb, H. (2022). Obese Visceral Fat Tissue Inflammation: From Protective to Detrimental?. BMC Med., 20.","DOI":"10.1186\/s12916-022-02672-y"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mam.2012.10.001","article-title":"Adipose Tissue Heterogeneity: Implication of Depot Differences in Adipose Tissue for Obesity Complications","volume":"34","author":"Lee","year":"2013","journal-title":"Mol. Asp. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.cmet.2017.12.002","article-title":"Anatomical, Physiological and Functional Diversity of Adipose Tissue","volume":"27","author":"Zwick","year":"2018","journal-title":"Cell Metab."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"E169","DOI":"10.1152\/ajpendo.00558.2020","article-title":"Fight against Fibrosis in Adipose Tissue Remodeling","volume":"321","author":"Li","year":"2021","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1007\/s12020-024-03734-2","article-title":"Liraglutide Induced Browning of Visceral White Adipose through Regulation of miRNAs in High-Fat-Diet-Induced Obese Mice","volume":"85","author":"Zhao","year":"2024","journal-title":"Endocrine"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Mandal, M., Mamun, M.A.A., Rakib, A., and Singh, U.P. (2025). High-Fat Diet-Induced Adipose Tissue-Resident Macrophages, T Cells, and Dendritic Cells Modulate Chronic Inflammation and Adipogenesis during Obesity. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1524544"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1038\/s41419-021-03872-9","article-title":"Adipocyte Death Triggers a Pro-Inflammatory Response and Induces Metabolic Activation of Resident Macrophages","volume":"12","author":"Lindhorst","year":"2021","journal-title":"Cell Death Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e230180","DOI":"10.1530\/JOE-23-0180","article-title":"Adipose Tissue Fibrosis: The Unwanted Houseguest Invited by Obesity","volume":"259","author":"Gliniak","year":"2023","journal-title":"J. Endocrinol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"114723","DOI":"10.1016\/j.bcp.2021.114723","article-title":"White Adipose Tissue Dysfunction in Obesity and Aging","volume":"192","author":"Serra","year":"2021","journal-title":"Biochem. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1186\/s12986-020-00513-4","article-title":"Chronic Stress, Epigenetics, and Adipose Tissue Metabolism in the Obese State","volume":"17","author":"Xiao","year":"2020","journal-title":"Nutr. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Duque-Colorado, J., Rivadeneira, J., and V\u00e1squez, B. (2025). Maternal Separation and Negative Renal Programming, Evidence of Morphofunctional Alterations in Rodent Models: Systematic Review and Meta-Analysis. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms262110509"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"E852","DOI":"10.1152\/ajpendo.00243.2020","article-title":"Exacerbated Obesogenic Response in Female Mice Exposed to Early Life Stress Is Linked to Fat Depot-Specific Upregulation of Leptin Protein Expression","volume":"319","author":"Leachman","year":"2020","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"112964","DOI":"10.1016\/j.physbeh.2020.112964","article-title":"Adverse Childhood Experiences and Adult Obesity: A Systematic Review of Plausible Mechanisms and Meta-Analysis of Cross-Sectional Studies","volume":"223","author":"Wiss","year":"2020","journal-title":"Physiol. Behav."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e23114","DOI":"10.1016\/j.heliyon.2023.e23114","article-title":"Adipose Tissue Inflammation Linked to Obesity: A Review of Current Understanding, Therapies and Relevance of Phyto-Therapeutics","volume":"10","author":"Aruwa","year":"2024","journal-title":"Heliyon"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1038\/s41590-023-01479-0","article-title":"Macrophage Function in Adipose Tissue Homeostasis and Metabolic In-flammation","volume":"24","author":"Chavakis","year":"2023","journal-title":"Nat. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/s12986-022-00694-0","article-title":"Browning of the White Adipose Tissue Regulation: New Insights into Nutritional and Metabolic Relevance in Health and Diseases","volume":"19","author":"Machado","year":"2022","journal-title":"Nutr. Metab."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Ziqubu, K., Dludla, P.V., Mthembu, S.X.H., Nkambule, B.B., Mabhida, S.E., Jack, B.U., Nyambuya, T.M., and Mazibuko-Mbeje, S.E. (2023). An Insight into Brown\/Beige Adipose Tissue Whitening, a Metabolic Complication of Obesity with the Multifactorial Origin. Front. Endocrinol., 14.","DOI":"10.3389\/fendo.2023.1114767"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4067\/s0717-95022026000100009","article-title":"Effects of Maternal Separation on Lipid Homeostasis and Visceral Adipose Tissue Organization in Mice Fed a High-Fat Diet Post-Weaning","volume":"44","author":"Navarrete","year":"2026","journal-title":"Int. J. Morphol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"595","DOI":"10.4093\/dmj.2023.0011","article-title":"Adipose Tissue and Metabolic Health","volume":"47","author":"An","year":"2023","journal-title":"Diabetes Metab. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1016\/j.it.2019.07.006","article-title":"Innate Immune Control of Adipose Tissue Homeostasis","volume":"40","author":"Kane","year":"2019","journal-title":"Trends Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2910","DOI":"10.2337\/db07-0767","article-title":"Adipocyte Death, Adipose Tissue Remodeling, and Obesity Complications","volume":"56","author":"Strissel","year":"2007","journal-title":"Diabetes"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Blade, S.P., Falkowski, D.J., Bachand, S.N., Pagano, S.J., and Chin, L. (2024). Mechanobiology of Adipocytes. Biology, 13.","DOI":"10.3390\/biology13060434"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"jeb164970","DOI":"10.1242\/jeb.164970","article-title":"Adipose Morphology and Metabolic Disease","volume":"221","author":"Tandon","year":"2018","journal-title":"J. Exp. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/j.cmet.2013.06.016","article-title":"Fibrosis and Adipose Tissue Dysfunction","volume":"18","author":"Sun","year":"2013","journal-title":"Cell Metab."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1194\/jlr.M038638","article-title":"Obese Adipocytes Show Ultrastructural Features of Stressed Cells and Die of Pyroptosis","volume":"54","author":"Giordano","year":"2013","journal-title":"J. Lipid Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"193","DOI":"10.2152\/jmi.64.193","article-title":"Adipocyte Death and Chronic Inflammation in Obesity","volume":"64","author":"Kuroda","year":"2017","journal-title":"J. Med. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5086","DOI":"10.1007\/s11695-020-04983-6","article-title":"Adipose Morphology: A Critical Factor in Regulation of Human Metabolic Diseases and Adipose Tissue Dysfunction","volume":"30","author":"Liu","year":"2020","journal-title":"Obes. Surg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.1194\/jlr.M800019-JLR200","article-title":"Dead Adipocytes, Detected as Crown-like Structures, Are Prevalent in Visceral Fat Depots of Genetically Obese Mice","volume":"49","author":"Murano","year":"2008","journal-title":"J. Lipid Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"101862","DOI":"10.1016\/j.molmet.2023.101862","article-title":"Activation of a Non-Neuronal Cholinergic System in Visceral White Adipose Tissue of Obese Mice and Humans","volume":"79","author":"Severi","year":"2024","journal-title":"Mol. Metab."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.bcp.2016.05.005","article-title":"Adipose Extracellular Matrix Remodelling in Obesity and Insulin Resistance","volume":"119","author":"Lin","year":"2016","journal-title":"Biochem. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"112962","DOI":"10.1016\/j.intimp.2024.112962","article-title":"Obesity-Derived Macrophages Upregulate TNF-\u03b1 to Induce Apoptosis in Glial Cell via the NF-\u03baB\/PHLPP1 Axis","volume":"141","author":"Ding","year":"2024","journal-title":"Int. Immunopharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"110029","DOI":"10.1016\/j.abb.2024.110029","article-title":"Single-Nucleus RNA Transcriptome Profiling Reveals Murine Adipose Tissue Endothelial Cell Proliferation Gene Networks Involved in Obesity Development","volume":"757","author":"Lu","year":"2024","journal-title":"Arch. Biochem. Biophys."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ruiz-Ojeda, F.J., M\u00e9ndez-Guti\u00e9rrez, A., Aguilera, C.M., and Plaza-D\u00edaz, J. (2019). Extracellular Matrix Remodeling of Adipose Tissue in Obesity and Metabolic Diseases. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20194888"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"de Sousa Neto, I.V., Durigan, J.L.Q., da Silva, A.S.R., and de C\u00e1ssia Marqueti, R. (2022). Adipose Tissue Extracellular Matrix Remodeling in Response to Dietary Patterns and Exercise: Molecular Landscape, Mechanistic Insights, and Therapeutic Approaches. Biology, 11.","DOI":"10.3390\/biology11050765"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"120718","DOI":"10.1016\/j.lfs.2022.120718","article-title":"Early Life Stress Exacerbates Obesity in Adult Female Mice via Mineralocorticoid Receptor-Dependent Increases in Adipocyte Triglyceride and Glycerol Content","volume":"304","author":"Leachman","year":"2022","journal-title":"Life Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Ruigrok, S.R., St\u00f6berl, N., Yam, K.-Y., de Lucia, C., Lucassen, P.J., Thuret, S., and Korosi, A. (2021). Modulation of the Hypothalamic Nutrient Sensing Pathways by Sex and Early-Life Stress. Front. Neurosci., 15.","DOI":"10.3389\/fnins.2021.695367"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"load021","DOI":"10.1093\/lifemeta\/load021","article-title":"Extracellular Matrix Remodelling in Obesity and Metabolic Disorders","volume":"2","author":"Musale","year":"2023","journal-title":"Life Metab."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Orozco, L., Rivadeneira, J., and V\u00e1squez, B. (2025). Impact of Early Postnatal Maternal Separation Stress on Pancreatic Function in Rodents: A Systematic Review and Meta-Analysis. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26209927"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"E1016","DOI":"10.1152\/ajpendo.00329.2010","article-title":"Adipose Tissue Macrophages in Insulin-Resistant Subjects Are Associated with Collagen VI and Fibrosis and Demonstrate Alternative Activation","volume":"299","author":"Spencer","year":"2010","journal-title":"Am. J. Physiol.-Endocrinol. Metab."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1007\/s12272-021-01350-6","article-title":"Mouse Model of the Adipose Organ: The Heterogeneous Anatomical Characteristics","volume":"44","author":"Chun","year":"2021","journal-title":"Arch. Pharmacal Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"56","DOI":"10.2337\/db10-0697","article-title":"The Portal Theory Supported by Venous Drainage\u2013Selective Fat Transplantation","volume":"60","author":"Rytka","year":"2011","journal-title":"Diabetes"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"del Sol, M., Navarrete, J., Garc\u00eda-Orozco, L., Duque-Colorado, J., S\u00f3cola-Barsallo, Z., Sandoval, C., and V\u00e1squez, B. (2025). When Timing Matters: Effects of Maternal Separation and Post-Weaning High-Fat Diet on Liver Morphology in a Rodent Model. Nutrients, 17.","DOI":"10.3390\/nu17101619"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1721727X231154152","DOI":"10.1177\/1721727X231154152","article-title":"Pancreatic Beta-Cell Dysfunction in Type 2 Diabetes","volume":"21","author":"Khin","year":"2023","journal-title":"Eur. J. Inflamm."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e230313","DOI":"10.1530\/JOE-23-0313","article-title":"An Adipocentric Perspective of Pancreatic Lipotoxicity in Diabetes Pathogenesis","volume":"262","author":"Risi","year":"2024","journal-title":"J. Endocrinol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"R353","DOI":"10.1152\/ajpregu.00216.2022","article-title":"High-Fat\/High-Sucrose Diet Worsens Metabolic Outcomes and Widespread Hypersensitivity Following Early-Life Stress Exposure in Female Mice","volume":"324","author":"Frick","year":"2023","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s11154-021-09687-5","article-title":"Leptin as a Key Regulator of the Adipose Organ","volume":"23","author":"Palou","year":"2022","journal-title":"Rev. Endocr. Metab. Disord."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Francisco, V., Pino, J., Campos-Cabaleiro, V., Ruiz-Fern\u00e1ndez, C., Mera, A., Gonzalez-Gay, M.A., G\u00f3mez, R., and Gualillo, O. (2018). Obesity, Fat Mass and Immune System: Role for Leptin. Front. Physiol., 9.","DOI":"10.3389\/fphys.2018.00640"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.psyneuen.2016.12.012","article-title":"Exposure to Chronic Early-Life Stress Lastingly Alters the Adipose Tissue, the Leptin System and Changes the Vulnerability to Western-Style Diet Later in Life in Mice","volume":"77","author":"Yam","year":"2017","journal-title":"Psychoneuroendocrinology"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Bouillon-Minois, J.-B., Trousselard, M., Thivel, D., Benson, A.C., Schmidt, J., Moustafa, F., Bouvier, D., and Dutheil, F. (2021). Leptin as a Biomarker of Stress: A Systematic Review and Meta-Analysis. Nutrients, 13.","DOI":"10.3390\/nu13103350"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"e4641","DOI":"10.7717\/peerj.4641","article-title":"Collagen Overlays Can Inhibit Leptin and Adiponectin Secretion but Not Lipid Accumulation in Adipocytes","volume":"6","author":"Christian","year":"2018","journal-title":"PeerJ"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2362","DOI":"10.1111\/febs.16176","article-title":"AgRP Neurons: Regulators of Feeding, Energy Expenditure, and Behavior","volume":"289","author":"Deem","year":"2022","journal-title":"FEBS J."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"4646","DOI":"10.1038\/s41467-024-48885-y","article-title":"AgRP Neuron Cis-Regulatory Analysis across Hunger States Reveals That IRF3 Mediates Leptin\u2019s Acute Effects","volume":"15","author":"Heyward","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1093\/icb\/icad119","article-title":"Minireview: Glucocorticoid\u2212Leptin Crosstalk: Role of Glucocorticoid\u2013Leptin Counterregulation in Metabolic Homeostasis and Normal Development","volume":"63","author":"Paul","year":"2023","journal-title":"Integr. Comp. Biol."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Li, X., Ren, Y., Chang, K., Wu, W., Griffiths, H.R., Lu, S., and Gao, D. (2023). Adipose Tissue Macrophages as Potential Targets for Obesity and Metabolic Diseases. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1153915"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2347","DOI":"10.1194\/jlr.M500294-JLR200","article-title":"Adipocyte Death Defines Macrophage Localization and Function in Adipose Tissue of Obese Mice and Humans","volume":"46","author":"Cinti","year":"2005","journal-title":"J. Lipid Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2207152","DOI":"10.1002\/advs.202207152","article-title":"Human RSPO1 Mutation Represses Beige Adipocyte Thermogenesis and Contributes to Diet-Induced Adiposity","volume":"10","author":"Sun","year":"2023","journal-title":"Adv. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Dimitrov, I., Stankova, T., Angelova, P., Boyadjiev, N., Georgieva, K., Dimov, I., Bivolarska, A., Draganova, M., Gerginska, F., and Daskalova, E. (2024). Diet-Induced Early Inflammatory Response of Visceral Adipose Tissue in Healthy Male Wistar Rats. Nutrients, 16.","DOI":"10.3390\/nu16081184"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Yao, J., Wu, D., and Qiu, Y. (2022). Adipose Tissue Macrophage in Obesity-Associated Metabolic Diseases. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.977485"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.molmet.2015.10.005","article-title":"Stress-Induced Activation of Brown Adipose Tissue Prevents Obesity in Conditions of Low Adaptive Thermogenesis","volume":"5","author":"Razzoli","year":"2015","journal-title":"Mol. Metab."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Maniam, J., Antoniadis, C., and Morris, M.J. (2014). Early-Life Stress, HPA Axis Adaptation, and Mechanisms Contributing to Later Health Outcomes. Front. Endocrinol., 5.","DOI":"10.3389\/fendo.2014.00073"},{"key":"ref_64","unstructured":"Russell, W.M.S., and Burch, R.L. (1992). The Principles of Humane Experimental Technique, Universities Federation for Animal Welfare."},{"key":"ref_65","unstructured":"Canadian Council on Animal Care (1992). Guide to the Care and Use of Experimental Animals, Canadian Council on Animal Care. [2nd ed.]. Available online: https:\/\/ccac.ca\/Documents\/Standards\/Guidelines\/Guide_to_the_Care_and_Use_of_Experimental_Animals_Vol1.pdf."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1939","DOI":"10.1093\/jn\/123.11.1939","article-title":"AIN-93 Purified Diets for Laboratory Rodents: Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet","volume":"123","author":"Reeves","year":"1993","journal-title":"J. Nutr."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/laban0109-35","article-title":"A Method for Gender Determination in Newborn Dark Pigmented Mice","volume":"38","author":"Meerding","year":"2009","journal-title":"Lab Anim."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"George, E.D., Bordner, K.A., Elwafi, H.M., and Simen, A.A. (2010). Maternal Separation with Early Weaning: A Novel Mouse Model of Early Life Neglect. BMC Neurosci., 11.","DOI":"10.1186\/1471-2202-11-123"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1136\/vr.116.16.431","article-title":"Guidelines on the Recognition of Pain, Distress and Discomfort in Experimental Animals and an Hypothesis for Assessment","volume":"116","author":"Morton","year":"1985","journal-title":"Vet. Rec."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1002\/dev.20292","article-title":"Effects of Early Deprivation and Maternal Separation on Pup-Directed Behavior and HPA Axis Measures in the Juvenile Female Rat","volume":"50","author":"Rees","year":"2008","journal-title":"Dev. Psychobiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"327","DOI":"10.4067\/S0717-95022021000100327","article-title":"Nutritional Research and Fetal Programming: Parental Nutrition Influences the Structure and Function of the Organs","volume":"39","author":"Aguila","year":"2021","journal-title":"Int. J. Morphol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"L148","DOI":"10.1152\/ajplung.1990.258.4.L148","article-title":"Recent Stereological Methods for Cell Biology: A Brief Survey","volume":"258","author":"Weibel","year":"1990","journal-title":"Am. J. Physiol.-Lung Cell. Mol. Physiol."},{"key":"ref_73","unstructured":"Institute for Laboratory Animal Research (2011). Guide for the Care and Use of Laboratory Animals, National Academies Press."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/11\/5056\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T13:52:15Z","timestamp":1780494735000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/11\/5056"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,3]]},"references-count":73,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ijms27115056"],"URL":"https:\/\/doi.org\/10.3390\/ijms27115056","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,3]]}}}