{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:13:17Z","timestamp":1785420797851,"version":"3.56.0"},"reference-count":42,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260822"],"award-info":[{"award-number":["82260822"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82260822"],"award-info":[{"award-number":["82260822"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Medical and Health Science and Technology Special Project of Ordos Municipal Health Commission","award":["WSKJ20250002"],"award-info":[{"award-number":["WSKJ20250002"]}]},{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia","doi-asserted-by":"crossref","award":["2024QN08026"],"award-info":[{"award-number":["2024QN08026"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Youth Top-notch Talent Foundation of Inner Mongolia","award":["[2024] No. 3"],"award-info":[{"award-number":["[2024] No. 3"]}]},{"name":"Key Research and Development Program of Ordos","award":["YF20232312"],"award-info":[{"award-number":["YF20232312"]}]},{"name":"Science and Technology Program of Ordos","award":["2022YY010"],"award-info":[{"award-number":["2022YY010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Allergic rhinitis (AR) is one of the most prevalent allergic disorders worldwide. Current pharmacological treatments are often limited by suboptimal efficacy and notable adverse effects. Herbal medicines, with their multi-component and multi-target therapeutic characteristics, have attracted increasing attention. Artemisia ordosica Krasch. (AOK), a traditional Chinese\/Mongolian medicine has demonstrated immunomodulatory, antioxidant, and anti-inflammatory activities. The anti-AR potential of AOK extract fractions was evaluated using in vitro mast cell degranulation inhibition assays, network pharmacology analysis, molecular docking, and molecular dynamics simulations to elucidate underlying pharmacological mechanisms. The P815 mast cell model induced by compound 48\/80 was employed to assess the inhibitory activity and cytotoxicity of different extract fractions. Among the tested fractions, the ethyl acetate fraction exhibited the most potent inhibitory effect on mast cell degranulation without significant cytotoxicity. Network pharmacology analysis identified 254 potential AR-related targets of AOK, with Signal Transducer and Activator of Transcription 3(STAT3), Src protein(SRC), Tumor protein 53(TP53), AKT Serine\/Threonine Kinase 1(AKT1), Heat Shock Protein 90 Alpha Family Class A Member 1(HSP90AA1), Estrogen Receptor 1(ESR1), and Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha(PIK3CA) identified as key hub proteins. Gene Ontology and KEGG pathway enrichment analyses indicated that AOK primarily modulated inflammatory and oxidative stress-related processes through the lipid and atherosclerosis, hypoxia-inducible factor-1, and AGE-RAGE signaling pathways. Molecular docking and dynamics simulations demonstrated strong binding affinities and stable interactions between major active constituents, particularly hydroxygenkwanin, and key targets such as SRC. The ethyl acetate fraction of AOK extract exhibited significant mast cell degranulation inhibitory activity, likely mediated via a synergistic multi-component, multi-target mechanism involving regulation of inflammatory and immune-related signaling pathways. These findings provide a pharmacological basis for the potential application of AOK in AR treatment.<\/jats:p>","DOI":"10.3390\/ijms27115122","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T17:18:16Z","timestamp":1780679896000},"page":"5122","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Study of Artemisia ordosica Krasch. Against Allergic Rhinitis Based on the P815 Mast Cell Degranulation Model, Network Pharmacology, Molecular Docking, and Molecular Dynamics"],"prefix":"10.3390","volume":"27","author":[{"given":"Mengmeng","family":"Wang","sequence":"first","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"},{"name":"General Clinical Research Center, Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos 017000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingming","family":"Zou","sequence":"additional","affiliation":[{"name":"General Clinical Research Center, Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos 017000, China"},{"name":"Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"},{"name":"General Clinical Research Center, Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos 017000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianxiang","family":"Bai","sequence":"additional","affiliation":[{"name":"General Clinical Research Center, Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos 017000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Wu","sequence":"additional","affiliation":[{"name":"General Clinical Research Center, Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos 017000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawei","family":"Hu","sequence":"additional","affiliation":[{"name":"General Clinical Research Center, Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos 017000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Han","sequence":"additional","affiliation":[{"name":"General Clinical Research Center, Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos 017000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Han","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Xiao","sequence":"additional","affiliation":[{"name":"Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"},{"name":"General Clinical Research Center, Ordos School of Clinical Medicine, Inner Mongolia Medical University, Ordos 017000, China"},{"name":"Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1111\/all.70216","article-title":"Prevalence of Allergic Rhinitis in China Continues to Increase Without Reaching a Plateau: An Analysis of Three Waves of National Surveys from 2005 to 2019","volume":"81","author":"Pu","year":"2026","journal-title":"Allergy"},{"key":"ref_2","first-page":"7165627","article-title":"Prevalence and Risk Factors for Allergic Rhinitis in China: A Systematic Review and Meta-Analysis","volume":"2022","author":"Pang","year":"2022","journal-title":"Evid.-Based Complement. 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