{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T03:57:51Z","timestamp":1781668671643,"version":"3.54.5"},"reference-count":73,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Chilean Agency for Research and Development"},{"name":"FONDECYT Postdoctorado","award":["3220470"],"award-info":[{"award-number":["3220470"]}]},{"name":"SIA","award":["85220025"],"award-info":[{"award-number":["85220025"]}]},{"name":"FONDECYT","award":["1251144"],"award-info":[{"award-number":["1251144"]}]},{"name":"FONDECYT de Iniciaci\u00f3n","award":["11250842"],"award-info":[{"award-number":["11250842"]}]},{"name":"FONDECYT Postdoctorado","award":["3250259"],"award-info":[{"award-number":["3250259"]}]},{"name":"ANID Doctorate Fellowship","award":["21211147"],"award-info":[{"award-number":["21211147"]}]},{"name":"Hub de microscopia UV","award":["EQM230155"],"award-info":[{"award-number":["EQM230155"]}]},{"name":"FONDECYT","award":["1241048"],"award-info":[{"award-number":["1241048"]}]},{"name":"Proyecto Milenio CINV","award":["ICN2025_026"],"award-info":[{"award-number":["ICN2025_026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Chronic wounds are characterized by persistent inflammation and impaired fibroblast function, leading to defective tissue repair. Pannexin-1 (Panx1) channels regulate extracellular ATP release and purinergic signaling, processes implicated in inflammation and regeneration. Probenecid (PBN), a clinically approved Panx1 channels inhibitor, has emerged as a potential therapeutic modulator in chronic wounds; however, its compatibility with growth factor-mediated regenerative responses remains unclear. Here, we evaluated whether pharmacological inhibition of Panx1 channels alters \u03b2FGF-induced responses in human neonatal dermal fibroblasts (HDFn). Cells were treated with \u03b2FGF (10 ng\/mL) in the presence or absence of PBN (200 \u03bcM), and migration, proliferation, extracellular matrix-related gene expression, extracellular ATP release, intracellular Ca2+ signaling, and Panx1 expression\/localization were assessed. PBN did not significantly alter \u03b2FGF-induced migration, proliferation, or most extracellular matrix-related transcriptional responses, except for a further reduction in COL1A1 expression. In addition, \u03b2FGF did not modify PANX1 transcript levels, Panx1 protein abundance, or membrane localization. Although \u03b2FGF reduced extracellular ATP release and transiently modulated ATP-induced Ca2+ signaling, these effects occurred without detectable changes in Panx1 expression or localization. These findings support the compatibility of PBN with \u03b2FGF-driven regenerative responses in HDFn cells.<\/jats:p>","DOI":"10.3390\/ijms27125155","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T05:43:46Z","timestamp":1780897426000},"page":"5155","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Probenecid-Mediated Pannexin-1 Inhibition Preserves \u03b2FGF-Driven Regenerative Responses in Human Dermal Fibroblasts"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5789-7490","authenticated-orcid":false,"given":"Ricardo","family":"Ceriani","sequence":"first","affiliation":[{"name":"Laboratorio de Innovaci\u00f3n Terap\u00e9utica y Diagn\u00f3stico Molecular, Escuela de Qu\u00edmica y Farmacia, Facultad de Farmacia, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Centro para la Investigaci\u00f3n Traslacional en Neurofarmacolog\u00eda (CITNE), Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Maripillan","sequence":"additional","affiliation":[{"name":"Laboratorio de Innovaci\u00f3n Terap\u00e9utica y Diagn\u00f3stico Molecular, Escuela de Qu\u00edmica y Farmacia, Facultad de Farmacia, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Laboratorio de Comunicaci\u00f3n Intercelular, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Centro Interdisciplinario de Neurociencia de Valpara\u00edso, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefany","family":"Ordenes","sequence":"additional","affiliation":[{"name":"Laboratorio de Comunicaci\u00f3n Intercelular, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Centro Interdisciplinario de Neurociencia de Valpara\u00edso, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Synaptopathies Laboratory, Facultad de Medicina, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Programa de Doctorado en Ciencias, Menci\u00f3n Neurociencia, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5472-8521","authenticated-orcid":false,"given":"Carolina","family":"Flores-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Laboratorio de Comunicaci\u00f3n Intercelular, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Centro Interdisciplinario de Neurociencia de Valpara\u00edso, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Facultad de Ciencias Fisiol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Santiago de Chile, Santiago 8320000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6870-8099","authenticated-orcid":false,"given":"Joel","family":"Novoa-Molina","sequence":"additional","affiliation":[{"name":"Laboratorio de Comunicaci\u00f3n Intercelular, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Centro Interdisciplinario de Neurociencia de Valpara\u00edso, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Programa de Doctorado en Ciencias, Menci\u00f3n Biof\u00edsica y Biolog\u00eda Computacional, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Tapia-Bustos","sequence":"additional","affiliation":[{"name":"Centro para la Investigaci\u00f3n Traslacional en Neurofarmacolog\u00eda (CITNE), Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5435-2587","authenticated-orcid":false,"given":"Caroline","family":"Weinstein-Oppenheimer","sequence":"additional","affiliation":[{"name":"Laboratorio de Innovaci\u00f3n Terap\u00e9utica y Diagn\u00f3stico Molecular, Escuela de Qu\u00edmica y Farmacia, Facultad de Farmacia, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Centro de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n en Productos Bioactivos (Cinbio), Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7842-8400","authenticated-orcid":false,"given":"Agust\u00edn D.","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Laboratorio de Comunicaci\u00f3n Intercelular, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"},{"name":"Centro Interdisciplinario de Neurociencia de Valpara\u00edso, Instituto de Neurociencia, Facultad de Ciencias, Universidad de Valpara\u00edso, Valpara\u00edso 2340000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1126\/scitranslmed.3009337","article-title":"Wound repair and regeneration: Mechanisms, signaling, and translation","volume":"6","author":"Eming","year":"2014","journal-title":"Sci. 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