{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T03:13:18Z","timestamp":1785208398929,"version":"3.55.0"},"reference-count":60,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Program for Key Basic Research Projects","award":["2022YFC2601400"],"award-info":[{"award-number":["2022YFC2601400"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["326QN0846"],"award-info":[{"award-number":["326QN0846"]}]},{"name":"Hainan Provincial Natural Science Foundation of China","award":["326QN0845"],"award-info":[{"award-number":["326QN0845"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The melon fly, Zeugodacus cucurbitae (Coquillett), is a globally significant agricultural pest causing substantial economic losses. Odorant-binding proteins (OBPs) are critical of the insect olfactory system, yet their specific physiological functions in Z. cucurbitae remain largely uncharacterized. In this study, we functionally characterized ZcucOBP14 and investigated its putative involvement in host chemoreception and insecticide tolerance. Sequence alignment and phylogenetic analysis indicated that ZcucOBP14 belongs to the Minus-C OBP subfamily, and quantitative reverse transcription PCR (RT-qPCR) showed that it was predominantly expressed in both the head and abdomen. Fluorescence binding assays revealed that ZcucOBP14 exhibited broad binding affinity to 11 host plant volatiles, three sex pheromones, and two insecticides. Subsequent electroantennography (EAG) and behavioral bioassays identified isopulegol, 1-hexanol, linalool, and \u03b1-pinene as key ligands regulating the behavioral responses of Z. cucurbitae. RNA interference (RNAi)-mediated knockdown of ZcucOBP14 significantly reduced EAG responses to key ligands, eliminated behavioral preference, and increased insecticide-induced mortality by 20%. Molecular docking further identified that Tyr71, Ile67, Trp50, Val107, Phe116 and Leu70 were critical residues involved in ligand interactions. Collectively, these findings highlight the indispensable role of ZcucOBP14 in olfactory perception and its contribution to insecticide tolerance, laying a solid theoretical foundation for the development of novel behavior-modifying agents, attractants, and optimized integrated pest management (IPM) strategies against this pest.<\/jats:p>","DOI":"10.3390\/ijms27125158","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T05:43:46Z","timestamp":1780897426000},"page":"5158","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Function and Mechanism of ZcucOBP14 in Regulating Olfactory Recognition and Insecticide Susceptibility in Zeugodacus cucurbitae"],"prefix":"10.3390","volume":"27","author":[{"given":"Jingjing","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"National Nan fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yue","sequence":"additional","affiliation":[{"name":"National Nan fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3636-8005","authenticated-orcid":false,"given":"Chao","family":"Ma","sequence":"additional","affiliation":[{"name":"National Nan fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenya","family":"Tian","sequence":"additional","affiliation":[{"name":"National Nan fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Nan fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2564-2176","authenticated-orcid":false,"given":"Hongsong","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory for Biology of Crop Diseases and Insect Pests, Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, Nanning 530007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Ma","sequence":"additional","affiliation":[{"name":"Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongshi","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"National Nan fan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572019, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1146\/annurev-ento-121423-013351","article-title":"Odorant Reception in Insects: Functional and Evolutionary Perspectives","volume":"71","author":"Leal","year":"2026","journal-title":"Annu. 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