{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:20:13Z","timestamp":1785406813484,"version":"3.56.0"},"reference-count":50,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010609","name":"Medical University of Gda\u0144sk","doi-asserted-by":"crossref","award":["ST 02\/130\/07\/308"],"award-info":[{"award-number":["ST 02\/130\/07\/308"]}],"id":[{"id":"10.13039\/501100010609","id-type":"DOI","asserted-by":"crossref"}]},{"award":["ST 02\/130\/07\/308"],"award-info":[{"award-number":["ST 02\/130\/07\/308"]}],"id":[{"id":"https:\/\/ror.org\/019sbgd69","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Human epidermal growth factor receptor 2 (HER2) remains the most recognized and clinically established molecular biomarker in gastric cancer; however, the regulatory mechanisms underlying its dysregulation are not fully understood. This study aimed to identify microRNAs associated with HER2 gene amplification, chromosome 17 centromere copy number increase (CNI), or alternative mechanisms driving HER2 protein overexpression. We analyzed 115 gastric cancer patients treated surgically at a single institution, with available material for immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and microRNA profiling. Among 11 candidate microRNAs, four demonstrated significant associations with HER2-related alterations. hsa-miR-128-3p expression was positively associated with HER2 gene amplification, while hsa-miR-145-5p expression showed an inverse relationship with centromere enumeration probe 17 (CEP17) signal count and correlated with membranous HER2 protein expression. hsa-miR-27b-5p expression was linked to CEP17 CNI, whereas hsa-miR-552-3p expression was associated with both increased HER2 amplification and CEP17 signal count. Importantly, hsa-miR-27b-5p upregulation independently predicted worse overall survival, whereas hsa-miR-128-3p upregulation independently predicted improved survival outcomes. These findings identify distinct microRNA signatures associated with HER2 pathway alterations and prognosis in gastric cancer, highlighting their potential as biomarkers and contributors to HER2-driven tumor biology.<\/jats:p>","DOI":"10.3390\/ijms27125184","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T10:04:14Z","timestamp":1780913054000},"page":"5184","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association of Selected miRNAs (hsa-miR-27b, hsa-miR-128-3p, hsa-miR-145-5p, hsa-miR-552-3p) with HER2 Status and Chromosome 17 Centromere Copy Number Increase in Gastric Cancer"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5935-0633","authenticated-orcid":false,"given":"Maciej","family":"Ciesielski","sequence":"first","affiliation":[{"name":"Department of Oncological Surgery, Gdynia Oncology Centre, 81-519 Gdynia, Poland"},{"name":"Department of Oncological Surgery, Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine, Medical University of Gda\u0144sk, 81-519 Gdynia, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7360-7810","authenticated-orcid":false,"given":"Marzena Anna","family":"Lewandowska","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Genetics Department, Innovative Medical Forum, \u0141ukaszczyk Oncology Centre, 85-796 Bydgoszcz, Poland"},{"name":"Department of Thoracic Surgery and Tumors, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, 85-067 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7595-7152","authenticated-orcid":false,"given":"Mariusz","family":"Szajewski","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Gdynia Oncology Centre, 81-519 Gdynia, Poland"},{"name":"Department of Oncological Surgery, Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine, Medical University of Gda\u0144sk, 81-519 Gdynia, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5562-0114","authenticated-orcid":false,"given":"Krzysztof","family":"Pastuszak","sequence":"additional","affiliation":[{"name":"Department of Algorithms and System Modelling, Gda\u0144sk University of Technology, 80-233 Gda\u0144sk, Poland"},{"name":"Laboratory of Translational Oncology, Intercollegiate Faculty of Biotechnology, University of Gda\u0144sk and Medical University of Gda\u0144sk, 80-307 Gda\u0144sk, Poland"},{"name":"Center of Biostatistics and Bioinformatics, Medical University of Gda\u0144sk, 80-211 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6799-5829","authenticated-orcid":false,"given":"Aleksandra","family":"Ciarka","sequence":"additional","affiliation":[{"name":"Department of Pathomorphology, Faculty of Medicine, Medical University of Gda\u0144sk, 80-210 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8580-536X","authenticated-orcid":false,"given":"Piotr","family":"Kurek","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Gdynia Oncology Centre, 81-519 Gdynia, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2553-4290","authenticated-orcid":false,"given":"Jakub","family":"Walczak","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Gdynia Oncology Centre, 81-519 Gdynia, Poland"},{"name":"Department of Oncological Surgery, Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine, Medical University of Gda\u0144sk, 81-519 Gdynia, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Sta\u0144czak","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Gdynia Oncology Centre, 81-519 Gdynia, Poland"},{"name":"Department of Oncological Surgery, Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine, Medical University of Gda\u0144sk, 81-519 Gdynia, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek","family":"Zieli\u0144ski","sequence":"additional","affiliation":[{"name":"2nd Division of Radiology, Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine, Medical University of Gda\u0144sk, 80-210 Gda\u0144sk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5929-5232","authenticated-orcid":false,"given":"Wies\u0142aw Janusz","family":"Kruszewski","sequence":"additional","affiliation":[{"name":"Department of Oncological Surgery, Gdynia Oncology Centre, 81-519 Gdynia, Poland"},{"name":"Department of Oncological Surgery, Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine, Medical University of Gda\u0144sk, 81-519 Gdynia, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"key":"ref_1","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1016\/j.annonc.2022.07.004","article-title":"Gastric cancer: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up","volume":"33","author":"Lordick","year":"2022","journal-title":"Ann. Oncol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s13643-025-02777-4","article-title":"Efficacy and safety of different drugs in patients with HER2-positive gastric cancer: Network meta-analysis","volume":"14","author":"Zhang","year":"2025","journal-title":"Syst. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Cheng, X. (2024). A Comprehensive Review of HER2 in Cancer Biology and Therapeutics. Genes, 15.","DOI":"10.20944\/preprints202406.0515.v1"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"142","DOI":"10.3892\/ol.2020.12403","article-title":"Impact of chromosome 17 centromere copy number increase on patient survival and human epidermal growth factor receptor 2 expression in gastric adenocarcinoma","volume":"21","author":"Ciesielski","year":"2021","journal-title":"Oncol. Lett."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e12854","DOI":"10.1097\/MD.0000000000012854","article-title":"The relationship between HER2 overexpression and angiogenesis in gastric cancer","volume":"97","author":"Ciesielski","year":"2018","journal-title":"Medicine"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4149","DOI":"10.2147\/OTT.S315252","article-title":"Recent Advances in Systemic Treatments for HER-2 Positive Advanced Gastric Cancer","volume":"14","author":"Kahraman","year":"2021","journal-title":"OncoTargets Ther."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/S0140-6736(10)61121-X","article-title":"Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): A phase 3, open-label, randomised controlled trial","volume":"376","author":"Bang","year":"2010","journal-title":"Lancet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1056\/NEJMra043186","article-title":"Trastuzumab\u2014Mechanism of Action and Use in Clinical Practice","volume":"357","author":"Hudis","year":"2007","journal-title":"N. Engl. J. Med."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Lote, H., Mousoullou, F., Vlachogiannis, G., Lampis, A., Satchwell, L., Peckitt, C., Fong, C., Begum, R., Kidd, S., and Cromarty, S. (2023). MicroRNAs as biomarkers for trastuzumab-based therapy in HER2-positive advanced oesophago-gastric cancer patients. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1258365"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1089\/hum.2024.98457","article-title":"The 2024 Nobel Prize: Impact of the Discovery of miRNA on the Field of Gene Therapy","volume":"36","author":"Mueller","year":"2025","journal-title":"Hum. Gene Ther."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Han, D.M., Wakefield, M.R., and Fang, Y. (2025). Beyond Detection: Conventional and Emerging Biomarkers in Gastrointestinal Cancers. Cancers, 17.","DOI":"10.3390\/cancers17172725"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.molmed.2014.06.005","article-title":"MicroRNAs in cancer: Biomarkers, functions and therapy","volume":"20","author":"Hayes","year":"2014","journal-title":"Trends Mol. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.1097\/CM9.0000000000000921","article-title":"Recent advances of miRNAs in the development and clinical application of gastric cancer","volume":"133","author":"Wu","year":"2020","journal-title":"Chin. Med. J."},{"key":"ref_15","first-page":"3807","article-title":"MicroRNA Signature for HER2-positive Breast and Gastric Cancer","volume":"34","author":"Kang","year":"2014","journal-title":"Anticancer Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1200\/JCO.2016.69.4836","article-title":"HER2 Testing and Clinical Decision Making in Gastroesophageal Adenocarcinoma: Guideline From the College of American Pathologists, American Society for Clinical Pathology, and the American Society of Clinical Oncology","volume":"35","author":"Bartley","year":"2017","journal-title":"J. Clin. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ciesielski, M., Lewandowska, M.A., Szajewski, M., Pastuszak, K., Kurek, P., Zieli\u0144ski, J., Walczak, J., P\u0119ksa, R., and Kruszewski, W.J. (2025). The Impact of miR-21-5p, miR-145-5p and miR-382-5p Expression in Gastric Adenocarcinoma Cells on Lymphatic Spread Capability. Biomedicines, 13.","DOI":"10.3390\/biomedicines13102393"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"403","DOI":"10.7150\/jca.11424","article-title":"Multiplex Ligation-dependent Probe Amplification Can Clarify HER2 Status in Gastric Cancers with \u201cPolysomy 17\u201d","volume":"6","author":"Wang","year":"2015","journal-title":"J. Cancer"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1038\/modpathol.2009.78","article-title":"Clinical validation of an array CGH test for HER2 status in breast cancer reveals that polysomy 17 is a rare event","volume":"22","author":"Yeh","year":"2009","journal-title":"Mod. Pathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.4103\/jcrt.JCRT_1111_19","article-title":"Underexpression of circulating miR-145-5p and miR-133a-3p are associated with breast cancer and immunohistochemical markers","volume":"16","year":"2020","journal-title":"J. Cancer Res. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.2147\/IJGM.S510358","article-title":"miR-145-5p Inhibits HER2-Positive Breast Cancer Cells via Targeting ARF6","volume":"18","author":"Yang","year":"2025","journal-title":"Int. J. Gen. Med."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4413","DOI":"10.21873\/anticanres.16637","article-title":"Melatonin Inhibits Gastric Cancer Cell Proliferation by Suppressing Exosome miR-27b-3p Expression","volume":"43","author":"Zhang","year":"2023","journal-title":"Anticancer. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1007\/s13238-016-0340-z","article-title":"miR-27b inhibits gastric cancer metastasis by targeting NR2F2","volume":"8","author":"Feng","year":"2017","journal-title":"Protein Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e595","DOI":"10.1002\/ctm2.595","article-title":"EMT-cancer cells-derived exosomal miR-27b-3p promotes circulating tumour cells-mediated metastasis by modulating vascular permeability in colorectal cancer","volume":"11","author":"Dou","year":"2021","journal-title":"Clin. Transl. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1186\/s13046-015-0253-3","article-title":"miR-27b-3p suppresses cell proliferation through targeting receptor tyrosine kinase like orphan receptor 1 in gastric cancer","volume":"34","author":"Tao","year":"2015","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_26","first-page":"5081","article-title":"Functional role of miR-27b in the development of gastric cancer","volume":"17","author":"Chen","year":"2018","journal-title":"Mol. Med. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4692","DOI":"10.3892\/mmr.2015.4002","article-title":"Relative levels of let-7a, miR-17, miR-27b, miR-125a, miR-125b and miR-206 as potential molecular markers to evaluate grade, receptor status and molecular type in breast cancer","volume":"12","author":"Lehmann","year":"2015","journal-title":"Mol. Med. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2884","DOI":"10.1158\/0008-5472.CAN-12-2162","article-title":"Prooncogenic factors miR-23b and miR-27b are regulated by Her2\/Neu, EGF, and TNF-\u03b1 in breast cancer","volume":"73","author":"Jin","year":"2013","journal-title":"Cancer Res."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Budi, H.S., Younus, L.A., Lafta, M.H., Parveen, S., Mohammad, H.J., Al-Qaim, Z.H., Jawad, M.A., Parra, R.M.R., Mustafa, Y.F., and Alhachami, F.R. (2022). The role of miR-128 in cancer development, prevention, drug resistance, and immunotherapy. Front. Oncol., 12.","DOI":"10.3389\/fonc.2022.1067974"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1016\/j.yexcr.2013.07.031","article-title":"miR-128 and its target genes in tumorigenesis and metastasis","volume":"319","author":"Li","year":"2013","journal-title":"Exp. Cell Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"108947","DOI":"10.1016\/j.biopha.2019.108947","article-title":"MiR-128-3p suppresses breast cancer cellular progression via targeting LIMK1","volume":"115","author":"Zhao","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Yan, H., Cai, X., Zhang, J., Zhao, H., Wu, H., Zhang, J., Xu, L., Liu, S., Zang, Y., and Fu, S. (2024). Gastric cancer cell-derived exosomal miRNA-128-3p promotes angiogenesis by targeting SASH1. Front. Oncol., 14.","DOI":"10.3389\/fonc.2024.1440996"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"37458","DOI":"10.18632\/oncotarget.6059","article-title":"MicroRNA-27b, microRNA-101 and microRNA-128 inhibit angiogenesis by down-regulating vascular endothelial growth factor C expression in gastric cancers","volume":"6","author":"Liu","year":"2015","journal-title":"Oncotarget"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s12957-023-02906-0","article-title":"microRNA-128-3p inhibits proliferation and accelerates apoptosis of gastric cancer cells via inhibition of TUFT1","volume":"21","author":"Du","year":"2023","journal-title":"World J. Surg. Oncol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.bbrc.2018.08.177","article-title":"MicroRNA-128 contributes to the progression of gastric carcinoma through GAREM-mediated MAPK signaling activation","volume":"504","author":"Han","year":"2018","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"42806","DOI":"10.1038\/srep42806","article-title":"Prognostic Value of microRNA Signature in Patients with Gastric Cancers","volume":"7","author":"Liu","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"6226","DOI":"10.7150\/jca.46613","article-title":"miR-552: An important post-transcriptional regulator that affects human cancer","volume":"11","author":"Zou","year":"2020","journal-title":"J. Cancer"},{"key":"ref_38","first-page":"412","article-title":"Increased expression of miR-552 acts as a potential predictor biomarker for poor prognosis of colorectal cancer","volume":"22","author":"Wang","year":"2018","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1159\/000505377","article-title":"Upregulation of miR-552 Predicts Unfavorable Prognosis of Gastric Cancer and Promotes the Proliferation, Migration, and Invasion of Gastric Cancer Cells","volume":"43","author":"Feng","year":"2020","journal-title":"Oncol. Res. Treat."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.molonc.2013.10.001","article-title":"High-throughput screens identify microRNAs essential for HER2 positive breast cancer cell growth","volume":"8","author":"Leivonen","year":"2014","journal-title":"Mol. Oncol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1074\/jbc.M609383200","article-title":"Coordinate Suppression of ERBB2 and ERBB3 by Enforced Expression of Micro-RNA miR-125a or miR-125b","volume":"282","author":"Scott","year":"2007","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1158\/1078-0432.CCR-10-2132","article-title":"MicroRNA-125a-5p Is an Independent Prognostic Factor in Gastric Cancer and Inhibits the Proliferation of Human Gastric Cancer Cells in Combination with Trastuzumab","volume":"17","author":"Nishida","year":"2011","journal-title":"Clin. Cancer Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.3892\/etm.2014.1627","article-title":"miR-375 inhibits the proliferation of gastric cancer cells by repressing ERBB2 expression","volume":"7","author":"Shen","year":"2014","journal-title":"Exp. Ther. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1002\/ijc.29168","article-title":"The sensitivity of gastric cancer to trastuzumab is regulated by the miR-223\/FBXW7 pathway","volume":"136","author":"Eto","year":"2015","journal-title":"Int. J. Cancer"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1245\/s10434-013-3325-7","article-title":"The MicroRNA-21\/PTEN Pathway Regulates the Sensitivity of HER2-Positive Gastric Cancer Cells to Trastuzumab","volume":"21","author":"Eto","year":"2014","journal-title":"Ann. Surg. Oncol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1038\/s41419-020-02946-4","article-title":"HOTAIR contributes to the carcinogenesis of gastric cancer via modulating cellular and exosomal miRNAs level","volume":"11","author":"Zhang","year":"2020","journal-title":"Cell Death Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1001\/jamasurg.2025.2493","article-title":"Exosomal liquid biopsy for the early detection of gastric cancer: The DESTINEX multicenter study","volume":"160","author":"Sui","year":"2025","journal-title":"JAMA Surg."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10213","DOI":"10.1245\/s10434-025-18088-w","article-title":"A liquid biopsy assay of exosomal miRNA for non-invasive identification of lymph node metastasis in early gastric cancer","volume":"32","author":"Wada","year":"2025","journal-title":"Ann. Surg. Oncol."},{"key":"ref_49","unstructured":"Amin, M.B., Greene, F.L., Edge, S.B., Compton, C.C., Gershenwald, J.E., Brookland, R.K., Meyer, L., Gress, D.M., Byrd, D.R., and Winchester, D.P. (2017). AJCC Cancer Staging Manual, American Joint Committee on Cancer."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1111\/j.1365-2559.2008.03028.x","article-title":"Assessment of a HER2 scoring system for gastric cancer: Results from a validation study","volume":"52","author":"Hofmann","year":"2008","journal-title":"Histopathology"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/12\/5184\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T04:23:33Z","timestamp":1781065413000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/12\/5184"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,8]]},"references-count":50,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ijms27125184"],"URL":"https:\/\/doi.org\/10.3390\/ijms27125184","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,8]]}}}