{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:26:18Z","timestamp":1785432378526,"version":"3.56.0"},"reference-count":76,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Manitoba, the Hoeschen Memorial Award 2024","award":["F328279"],"award-info":[{"award-number":["F328279"]}]},{"award":["F328279"],"award-info":[{"award-number":["F328279"]}],"id":[{"id":"https:\/\/ror.org\/02gfys938","id-type":"ROR","asserted-by":"publisher"}]},{"name":"the 2024 GFT Surgery Grant","award":["F327961"],"award-info":[{"award-number":["F327961"]}]},{"name":"the 2021 HSC Foundation Operating Grant","award":["R510837"],"award-info":[{"award-number":["R510837"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>One-lung ventilation (OLV) is performed during lung surgeries by ventilating a single lung, while collapsing the operative lung to provide surgical exposure within the thoracic cavity. While a lung-protective ventilation strategy is recommended during OLV, increasing the fraction of inspired oxygen (FiO2) or tidal volume (VT) may be required to prevent hypoxemia during surgery. Unfortunately, these increases are associated with postoperative lung injury. Using a porcine model of OLV, our project aims to determine if high FiO2 or VT during OLV contributes to elevation of pro-inflammatory lipid mediators postoperatively. Fifteen three-month-old farm-bred pigs underwent left upper lobectomy requiring OLV. Pigs were exposed to one of three ventilation parameters: normoxic low VT lung-protective ventilation LPV-NO, n = 5, FiO2 &lt; 50%, VT = 6 mL\/kg), hyperoxic lung-protective ventilation (LPV-HO, n = 5, FiO2 &gt;100%, VT = 6 mL\/kg), or normoxic high VT (injurious mechanical ventilation) (IMV, n = 5, FiO2 &lt; 50%, VT = 10\u201312 mL\/kg). Arterial plasma was collected before and after OLV, and lipids were detected via LC-MS-MS. Lipidomic analysis demonstrated a statistically significant increase (FC = 2, p \u2264 0.05) in lysophosphatidylethanolamines (LPE 18:3, LPE 20:4, LPE 18:2, LPE 22:6), free fatty acids (FFA 20:4), phosphatidylserine (PS 38:5), lysophosphatidylcholine (LPC 18:1, LPC 18:3, LPC 22:6), triglyceride (TG 18:2-18:2-20:4), free fatty acids (FFA 20:5), linoleyl-carnitine molecules (C18-2 Linoleoyl Carnitine), and phosphatidylethanolamines (PE 36:5) in LPV-HO. IMV resulted in a significant increase (FC = 2, p \u2264 0.05) in triglyceride (TG 18:2-18:2-20:4), diglyceride (DG 18:1-20:4, DG 16:0-20:4), linoleyl-carnitine molecules (C18-2 Linoleoyl Carnitine), and free fatty acids (FFA 20:5). There was no significant change in lipid biomarker levels following LPV-NO post-surgery. Our lipidomic analysis supports that both high FiO2 and VT contribute to systemic lipid metabolic derangements. Lipids that were elevated in LPV-HO and IMV are associated with multiple inflammatory pathways implicated in lung injury. This suggests that intra-operative anti-inflammatory therapies targeted to these lipid pathways may reduce or prevent postoperative pulmonary complications after lung surgery.<\/jats:p>","DOI":"10.3390\/ijms27125219","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:33:44Z","timestamp":1781073224000},"page":"5219","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Plasma Lipidomics Identify Pathways Linked to Acute Lung Injury in a Porcine One-Lung Ventilation Surgery Model"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3732-3781","authenticated-orcid":false,"given":"Simone C.","family":"da Silva Rosa","sequence":"first","affiliation":[{"name":"Biology of Breathing Research Theme, the Children\u2019s Hospital Research Institute of Manitoba, Winnipeg, MB R3E 3P4, Canada"},{"name":"Section of Thoracic Surgery, Department of Surgery, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3A 1R9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Gauvin","sequence":"additional","affiliation":[{"name":"Undergraduate Medical Education (UGME), Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 3P5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6001-7013","authenticated-orcid":false,"given":"Dagem","family":"Chernet","sequence":"additional","affiliation":[{"name":"Biology of Breathing Research Theme, the Children\u2019s Hospital Research Institute of Manitoba, Winnipeg, MB R3E 3P4, Canada"},{"name":"Department of Physiology and Pathophysiology, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2974-8533","authenticated-orcid":false,"given":"Jay","family":"Kormish","sequence":"additional","affiliation":[{"name":"Biology of Breathing Research Theme, the Children\u2019s Hospital Research Institute of Manitoba, Winnipeg, MB R3E 3P4, Canada"},{"name":"Section of Thoracic Surgery, Department of Surgery, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3A 1R9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Giffin","sequence":"additional","affiliation":[{"name":"Undergraduate Medical Education (UGME), Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 3P5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Hinton","sequence":"additional","affiliation":[{"name":"Undergraduate Medical Education (UGME), Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 3P5, Canada"},{"name":"Department of Physiology and Pathophysiology, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7279-5226","authenticated-orcid":false,"given":"Shyamala","family":"Dakshinamurti","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Perioperative and Pain Medicine, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0Z2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5229-9927","authenticated-orcid":false,"given":"Christopher D.","family":"Pascoe","sequence":"additional","affiliation":[{"name":"Biology of Breathing Research Theme, the Children\u2019s Hospital Research Institute of Manitoba, Winnipeg, MB R3E 3P4, Canada"},{"name":"Department of Physiology and Pathophysiology, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6663-1225","authenticated-orcid":false,"given":"Amir","family":"Ravandi","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"},{"name":"Institute of Cardiovascular Sciences, St. Boniface Hospital Research, University of Manitoba, Winnipeg, MB R2H 2A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2982-2505","authenticated-orcid":false,"given":"Biniam","family":"Kidane","sequence":"additional","affiliation":[{"name":"Biology of Breathing Research Theme, the Children\u2019s Hospital Research Institute of Manitoba, Winnipeg, MB R3E 3P4, Canada"},{"name":"Section of Thoracic Surgery, Department of Surgery, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3A 1R9, Canada"},{"name":"Department of Physiology and Pathophysiology, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3E 0J9, Canada"},{"name":"Department of Biomedical Engineering, Price Faculty of Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102693","DOI":"10.1016\/j.canep.2024.102693","article-title":"Global burden of lung cancer in 2022 and projections to 2050: Incidence and mortality estimates from GLOBOCAN","volume":"93","author":"Zhou","year":"2024","journal-title":"Cancer Epidemiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"E526","DOI":"10.1503\/cmaj.252152","article-title":"Projected estimates of cancer in Canada in 2026","volume":"198","author":"Brenner","year":"2026","journal-title":"Can. 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