{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:47:05Z","timestamp":1790185625513,"version":"4.1.0"},"reference-count":59,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T00:00:00Z","timestamp":1781136000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Associated with high morbidity and mortality, cisplatin-induced acute kidney injury (AKI) is a common clinical complication characterized by oxidative stress, inflammation, and mitochondria-associated signaling. Although multiple signaling pathways have been implicated in AKI progression, effective interventions targeting these complex mechanisms are still lacking. As a medicinal fungus with antioxidant and anti-inflammatory properties, Schizophyllum commune (SC) has shown potential biological activities; however, its renoprotective effects in cisplatin-induced AKI remain unclear. Therefore, this study aimed to investigate SC\u2019s protective effects and underlying mechanisms in a cisplatin-induced AKI mouse model. SC treatment improved renal function and attenuated histopathological damage. It reduced oxidative stress and inflammatory responses, as evidenced by the modulation of malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO), and pro-inflammatory cytokines. Mechanistically, SC regulated multiple signaling pathways, including mitogen-activated protein kinase (MAPK), toll-like receptor 4\/nuclear factor kappa B (TLR4\/ NF-\u03baB), PI3K\/AKT, nuclear factor erythroid 2\u2013related factor 2\/heme oxygenase-1 (Nrf2\/HO-1), and the calcium\/calmodulin-dependent protein kinase kinase\u2013AMP-activated protein kinase\u2013sirtuin 1 (CaMKK\u2013AMPK\u2013Sirt1) axis. In addition, SC modulated apoptosis, autophagy, and PTEN-induced kinase 1 (PINK1)\/Parkin-mediated mitophagy, suggesting improved mitochondrial homeostasis. These findings indicate that SC exerts renoprotective effects and may contribute to cisplatin-induced nephrotoxicity mitigation strategies.<\/jats:p>","DOI":"10.3390\/ijms27125302","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T06:53:10Z","timestamp":1781247190000},"page":"5302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cisplatin-Induced Nephrotoxicity Attenuation by Schizophyllum commune Through Regulating Mitochondria-Associated Signaling, Apoptosis, Autophagy, and PINK1\/Parkin-Mediated Mitophagy"],"prefix":"10.3390","volume":"27","author":[{"given":"Yu-Wen","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University, Taichung 404333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Te-Kai","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Pharmacy, China Medical University, Taichung 404333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Ping","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, China Medical University, Taichung 404333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9822-3485","authenticated-orcid":false,"given":"Guan-Jhong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Chinese Medicine, China Medical University, Taichung 404333, Taiwan"},{"name":"Department of Food Nutrition and Healthy Biotechnology, Asia University, Taichung 41354, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2427802","DOI":"10.1155\/2022\/2427802","article-title":"Several alkaloids in Chinese herbal medicine exert protection in acute kidney injury: Focus on mechanism and target analysis","volume":"2022","author":"Rui","year":"2022","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2393262","DOI":"10.1080\/0886022X.2024.2393262","article-title":"Mitochondrial dysfunction in acute kidney injury","volume":"46","author":"Yao","year":"2024","journal-title":"Ren. Fail."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"967826","DOI":"10.1155\/2014\/967826","article-title":"Pathophysiology of cisplatin-induced acute kidney injury","volume":"2014","author":"Ozkok","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"151466","DOI":"10.1016\/j.semnephrol.2023.151466","article-title":"Infections and acute kidney injury: A global perspective","volume":"43","author":"Batte","year":"2023","journal-title":"Semin. Nephrol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1038\/ki.2011.379","article-title":"Chronic kidney disease after acute kidney injury: A systematic review and meta-analysis","volume":"81","author":"Coca","year":"2012","journal-title":"Kidney Int."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Pande, C.K., Smith, M.B., Soranno, D.E., Gist, K.M., Fuhrman, D.Y., Dolan, K., Conroy, A.L., and Akcan-Arikan, A. (2022). The neglected price of pediatric acute kidney injury: Non-renal implications. Front. Pediatr., 10.","DOI":"10.3389\/fped.2022.893993"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Hussain, S., Singh, A., Antony, B., Claure-Del Granado, R., Klugarova, J., L\u00ed\u010den\u00edk, R., and Klugar, M. (2021). Association of acute kidney injury with the risk of dementia: A meta-analysis. J. Clin. Med., 10.","DOI":"10.1101\/2021.07.27.21261192"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.tox.2007.12.024","article-title":"Biomarkers of nephrotoxic acute kidney injury","volume":"245","author":"Ferguson","year":"2008","journal-title":"Toxicology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1517\/17425255.2010.497140","article-title":"Molecular-targeted approaches to reduce renal accumulation of nephrotoxic drugs","volume":"6","author":"Nagai","year":"2010","journal-title":"Expert Opin. Drug Metab. Toxicol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.2215\/CJN.11290821","article-title":"Drug-induced acute kidney injury","volume":"17","author":"Perazella","year":"2022","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0305-7372(98)90061-5","article-title":"Cumulative toxicities from cisplatin therapy and current cytoprotective measures","volume":"24","author":"Cvitkovic","year":"1998","journal-title":"Cancer Treat. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1007\/s11255-022-03418-8","article-title":"Cisplatin and AKI: An ongoing battle with new perspectives\u2014A narrative review","volume":"55","author":"Volovat","year":"2023","journal-title":"Int. Urol. Nephrol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1038\/sj.onc.1206933","article-title":"Cisplatin: Mode of cytotoxic action and molecular basis of resistance","volume":"22","author":"Siddik","year":"2003","journal-title":"Oncogene"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Holditch, S.J., Brown, C.N., Lombardi, A.M., Nguyen, K.N., and Edelstein, C.L. (2019). Recent advances in models, mechanisms, biomarkers, and interventions in cisplatin-induced acute kidney injury. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20123011"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1016\/j.ijbiomac.2019.11.183","article-title":"Characterization of physicochemical and biological properties of Schizophyllum commune polysaccharide extracted with different methods","volume":"156","author":"Chen","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"135326","DOI":"10.1016\/j.ijbiomac.2024.135326","article-title":"Schizophyllum commune fruiting body polysaccharides inhibit glioma by mediating ARHI regulation of PI3K\/AKT signalling pathway","volume":"279","author":"Zheng","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Sun, T.K., Huang, W.C., Sun, Y.W., Deng, J.S., Chien, L.H., Chou, Y.N., Jiang, W.P., Lin, J.G., and Huang, G.J. (2022). Schizophyllum commune reduces expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232314766"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Mi\u0161kovi\u0107, J., Karaman, M., Ra\u0161eta, M., Krsmanovi\u0107, N., Bere\u017eni, S., Jakovljevi\u0107, D., Piattoni, F., Zambonelli, A., Gargano, M.L., and Venturella, G. (2021). Comparison of two Schizophyllum commune strains in production of acetylcholinesterase inhibitors and antioxidants from submerged cultivation. J. Fungi, 7.","DOI":"10.3390\/jof7020115"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"105940","DOI":"10.1016\/j.fitote.2024.105940","article-title":"Bioguided isolation, identification and bioactivity evaluation of anti-fatigue constituents from Schizophyllum commune","volume":"175","author":"Chen","year":"2024","journal-title":"Fitoterapia"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Paterska, M., Czerny, B., and Cielecka-Piontek, J. (2024). Macrofungal extracts as a source of bioactive compounds for cosmetical anti-aging therapy: A comprehensive review. Nutrients, 16.","DOI":"10.3390\/nu16162810"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"124504","DOI":"10.1016\/j.ijbiomac.2023.124504","article-title":"Efficient production and skincare activity evaluation of schizophyllan, a \u03b2-glucan derived from Schizophyllum commune NTU-1","volume":"241","author":"Gou","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1016\/S1567-5769(01)00088-1","article-title":"The regulatory role of nitric oxide in apoptosis","volume":"1","author":"Kim","year":"2001","journal-title":"Int. Immunopharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"113779","DOI":"10.1016\/j.bcp.2019.113779","article-title":"Pyroptotic and apoptotic cell death in iNOS and nNOS overexpressing K562 cells: A mechanistic insight","volume":"176","author":"Sadaf","year":"2020","journal-title":"Biochem. Pharmacol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wang, P., Wu, Z., Song, Z., Deng, X., Zhang, Y., and Gong, X. (2026). Arsenic trioxide-induced acute kidney injury: OPA1-and Drp1-mediated mitochondrial dynamics imbalance, PINK1\/Parkin-dependent mitophagy, and Chuanhuang Fang III. Front. Mol. Biosci., 13.","DOI":"10.3389\/fmolb.2026.1778855"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1038\/s41419-018-1152-2","article-title":"PINK1\/Parkin-mediated mitophagy is activated in cisplatin nephrotoxicity to protect against kidney injury","volume":"9","author":"Wang","year":"2018","journal-title":"Cell Death Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101254","DOI":"10.1016\/j.redox.2019.101254","article-title":"PINK1-parkin pathway of mitophagy protects against contrast-induced acute kidney injury via decreasing mitochondrial ROS and NLRP3 inflammasome activation","volume":"26","author":"Lin","year":"2019","journal-title":"Redox Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1042\/CS20256705","article-title":"Double-edged mitophagy: Balancing inflammation and resolution in lung disease","volume":"139","author":"Tian","year":"2025","journal-title":"Clin. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1200\/JCO.1996.14.7.2101","article-title":"Amifostine pretreatment for protection against cyclophosphamide-induced and cisplatin-induced toxicities: Results of a randomized control trial in patients with advanced ovarian cancer","volume":"14","author":"Kemp","year":"1996","journal-title":"J. Clin. Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3333","DOI":"10.1200\/JCO.1999.17.10.3333","article-title":"American Society of Clinical Oncology Clinical Practice Guidelines for the Use of Chemotherapy and Radiotherapy Protectants","volume":"17","author":"Hensley","year":"1999","journal-title":"J. Clin. Oncol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1038\/nature07813","article-title":"AMPK regulates energy expenditure by modulating NAD+ metabolism and SIRT1 activity","volume":"458","author":"Feige","year":"2009","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cmet.2005.05.009","article-title":"Calmodulin-dependent protein kinase kinase-\u03b2 is an alternative upstream kinase for AMP-activated protein kinase","volume":"2","author":"Hawley","year":"2005","journal-title":"Cell Metab."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.cell.2017.04.001","article-title":"AKT\/PKB signaling: Navigating the network","volume":"169","author":"Manning","year":"2017","journal-title":"Cell"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chou, Y.N., Lee, M.M., Deng, J.S., Jiang, W.P., Lin, J.G., and Huang, G.J. (2023). Water extract from brown strain of Flammulina velutipes alleviates cisplatin-induced acute kidney injury by attenuating oxidative stress, inflammation, and autophagy via PI3K\/AKT pathway regulation. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24119448"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/sj.ki.5002702","article-title":"The phosphoinositide-3 kinase \u03b3\u2013Akt pathway mediates renal tubular injury in cisplatin nephrotoxicity","volume":"73","author":"Kuwana","year":"2008","journal-title":"Kidney Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.fct.2016.11.004","article-title":"Carvacrol attenuates acute kidney injury induced by cisplatin through suppression of ERK and PI3K\/Akt activation","volume":"98","year":"2016","journal-title":"Food Chem. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1080\/15548627.2017.1405880","article-title":"PINK1-PRKN\/PARK2 pathway of mitophagy is activated to protect against renal ischemia-reperfusion injury","volume":"14","author":"Tang","year":"2018","journal-title":"Autophagy"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1038\/sj.ki.5002786","article-title":"Cisplatin nephrotoxicity: Mechanisms and renoprotective strategies","volume":"73","author":"Pabla","year":"2008","journal-title":"Kidney Int."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"156550","DOI":"10.1016\/j.phymed.2025.156550","article-title":"Smilax glabra roxb. alleviates cisplatin-induced acute kidney injury in mice by activating the Nrf2\/HO-1 Signalling Pathway","volume":"139","author":"Zhao","year":"2025","journal-title":"Phytomedicine"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"7912763","DOI":"10.1155\/2020\/7912763","article-title":"Cordyceps cicadae mycelia ameliorate cisplatin-induced acute kidney injury by suppressing the TLR4\/NF-\u03baB\/MAPK and activating the HO-1\/Nrf2 and Sirt-1\/AMPK pathways in mice","volume":"2020","author":"Deng","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"114264","DOI":"10.1016\/j.intimp.2025.114264","article-title":"Cordycepin alleviates renal ischemia\u2013reperfusion injury by suppressing the p38\/JNK signaling pathway","volume":"150","author":"Chen","year":"2025","journal-title":"Int. Immunopharmacol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1007\/s11302-025-10107-5","article-title":"Adenosine receptors and acute kidney injury: Perspectives for future therapy","volume":"21","author":"Wissmann","year":"2025","journal-title":"Purinergic Signal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1038\/nrd1983","article-title":"Adenosine receptors as therapeutic targets","volume":"5","author":"Jacobson","year":"2006","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"S56","DOI":"10.1111\/j.1523-1755.2004.09109.x","article-title":"Inflammatory cytokines in acute renal failure","volume":"66","author":"Ramesh","year":"2004","journal-title":"Kidney Int."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Ray, A., and Gulati, K. (2023). Nitric Oxide: From Research to Therapeutics, Springer International Publishing. Advances in Biochemistry in Health and Disease.","DOI":"10.1007\/978-3-031-24778-1"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1681\/ASN.2014010117","article-title":"Beyond tissue injury\u2014Damage-associated molecular patterns, toll-like receptors, and inflammasomes also drive regeneration and fibrosis","volume":"25","author":"Anders","year":"2014","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1016\/j.bcp.2008.07.017","article-title":"Activation of Nrf2-antioxidant signaling attenuates NF\u03baB-inflammatory response and elicits apoptosis","volume":"76","author":"Li","year":"2008","journal-title":"Biochem. Pharmacol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Chien, L.H., Wu, C.T., Deng, J.S., Jiang, W.P., Huang, W.C., and Huang, G.J. (2021). Salvianolic acid C protects against cisplatin-induced acute kidney injury through attenuation of inflammation, oxidative stress, and apoptotic effects and activation of the CaMKK\u2013AMPK\u2013SIRT1-associated signaling pathway in mouse models. Antioxidants, 10.","DOI":"10.3390\/antiox10101620"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.cmet.2012.04.003","article-title":"SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function","volume":"15","author":"Price","year":"2012","journal-title":"Cell Metab."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Lin, W.H., Jiang, W.P., Chen, C.C., Lee, L.Y., Tsai, Y.S., Chien, L.H., Chou, Y.N., Deng, J.S., and Huang, G.J. (2022). Renoprotective effect of Pediococcus acidilactici GKA4 on cisplatin-induced acute kidney injury by mitigating inflammation and oxidative stress and regulating the MAPK, AMPK\/SIRT1\/NF-\u03baB, and PI3K\/AKT pathways. Nutrients, 14.","DOI":"10.3390\/nu14142877"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/nrm2308","article-title":"The BCL-2 protein family: Opposing activities that mediate cell death","volume":"9","author":"Youle","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"502676","DOI":"10.1155\/2014\/502676","article-title":"The impact of autophagy on cell death modalities","volume":"2014","author":"Ryter","year":"2014","journal-title":"Int. J. Cell Biol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Lee, M.M., Chou, Y.X., Huang, S.H., Cheng, H.T., Liu, C.H., and Huang, G.J. (2024). Renoprotective effects of brown-strain Flammulina velutipes Singer in chronic kidney disease-induced mice through modulation of oxidative stress and inflammation and regulation of renal transporters. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252212096"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"101767","DOI":"10.1016\/j.redox.2020.101767","article-title":"The PINK1\/PARK2\/optineurin pathway of mitophagy is activated for protection in septic acute kidney injury","volume":"38","author":"Wang","year":"2021","journal-title":"Redox Biol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"a011338","DOI":"10.1101\/cshperspect.a011338","article-title":"Mitochondrial quality control mediated by PINK1 and Parkin: Links to parkinsonism","volume":"4","author":"Narendra","year":"2012","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Ghosh, M., Thangada, S., Dasgupta, O., Khanna, K.M., Yamase, H.T., Kashgarian, M., Hla, T., Shapiro, L.H., and A Ferrer, F. (2018). Cell-intrinsic sphingosine kinase 2 promotes macrophage polarization and renal inflammation in response to unilateral ureteral obstruction. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194053"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"106260","DOI":"10.1016\/j.mimet.2021.106260","article-title":"Refinement of the Griess method for measuring nitrite in biological samples","volume":"187","author":"Franco","year":"2021","journal-title":"J. Microbiol. Methods"},{"key":"ref_57","first-page":"e61122","article-title":"Evaluation of oxidative stress in biological samples using the thiobarbituric acid reactive substances assay","volume":"159","author":"Borges","year":"2020","journal-title":"J. Vis. Exp."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Lin, J.G., Jiang, W.P., Tsai, Y.S., Lin, S.W., Chen, Y.L., Chen, C.C., and Huang, G.J. (2024). Dietary probiotic Pediococcus acidilactici GKA4, dead probiotic GKA4, and postbiotic GKA4 improves cisplatin-induced AKI by autophagy and endoplasmic reticulum stress and organic ion transporters. Nutrients, 16.","DOI":"10.3390\/nu16203532"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/0003-9861(59)90090-6","article-title":"Tissue sulfhydryl groups","volume":"82","author":"Ellman","year":"1959","journal-title":"Arch. Biochem. Biophys."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/12\/5302\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T04:23:32Z","timestamp":1781843012000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/12\/5302"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,11]]},"references-count":59,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ijms27125302"],"URL":"https:\/\/doi.org\/10.3390\/ijms27125302","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,11]]}}}