{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T09:53:23Z","timestamp":1781517203324,"version":"3.54.1"},"reference-count":74,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T00:00:00Z","timestamp":1781308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Carlos Chagas Filho Foundation of Research Support of the State of Rio de Janeiro","award":["FAPERJ #010.101.029\/2018"],"award-info":[{"award-number":["FAPERJ #010.101.029\/2018"]}]},{"name":"Brazilian Council for Scientific Research","award":["CNPq #301744\/2019-0"],"award-info":[{"award-number":["CNPq #301744\/2019-0"]}]},{"name":"MCTI (PNIPE)-FINEP","award":["#0918\/25"],"award-info":[{"award-number":["#0918\/25"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Brucellosis is a globally prevalent zoonotic disease affecting both humans and animals. Its nonspecific clinical manifestations often complicate diagnosis, underscoring the need for reliable laboratory confirmation. Traditional serological assays, though widely used, suffer from limitations such as inconsistent sensitivity and false-positive results. To address these challenges, this study mapped IgM and IgG epitopes of the Brucella Omp-2a protein using sera from infected patients. Epitope identification was performed through SPOT synthesis on cellulose membranes, followed by assessment of potential cross-reactivity using peptide database analysis and ELISA validation. Three major IgM and seven IgG linear B-cell epitopes were identified, six of which demonstrated strong reactivity in peptide-ELISA. Importantly, no significant cross-reactivity with proteins from other human pathogens was detected. Two chimeric multi-epitope peptides, composed of 50 and 60 amino acids and integrating Brucella-specific IgM and IgG epitopes, exhibited excellent diagnostic performance in ELISA, achieving near 100% sensitivity and specificity. These findings support the potential of synthetic peptides as reliable and cost-effective alternatives to native antigens in serological assays. Further validation in larger, geographically diverse cohorts will be essential to confirm their diagnostic robustness and facilitate their integration into routine brucellosis diagnostics.<\/jats:p>","DOI":"10.3390\/ijms27125341","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T08:57:58Z","timestamp":1781513878000},"page":"5341","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["IgM and IgG Epitope Mapping of the Porin Outer Membrane Protein-2a from Brucella abortus: Potential Biomarkers for Detecting Exposure to Brucellosis"],"prefix":"10.3390","volume":"27","author":[{"given":"Armando F.","family":"Noguera","sequence":"first","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil"},{"name":"Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4538-3802","authenticated-orcid":false,"given":"Guilherme C.","family":"Lechuga","sequence":"additional","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Napole\u00e3o-P\u00eago","sequence":"additional","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil"},{"name":"Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joao P. R. S.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niter\u00f3i 22040-036, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7194-0858","authenticated-orcid":false,"given":"Larissa R.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreia","family":"Carneiro da Silva","sequence":"additional","affiliation":[{"name":"Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Melo Monnerat","sequence":"additional","affiliation":[{"name":"Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7819-7654","authenticated-orcid":false,"given":"Flavio R.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2172-656X","authenticated-orcid":false,"given":"Salvatore G.","family":"De-Simone","sequence":"additional","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil"},{"name":"Technological Center of the Marine Corps (CTecFN), Brazilian Navy, Rio de Janeiro 21010-076, Brazil"},{"name":"Program of Post-Graduation on Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niter\u00f3i 22040-036, Brazil"},{"name":"Program of Post-Graduation on Parasitic Biology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2290839","DOI":"10.1080\/22221751.2023.2290839","article-title":"Long ignored but making a comeback: A worldwide epidemiological evolution of human brucellosis","volume":"13","author":"Liu","year":"2024","journal-title":"Emerg. 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