{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:10:52Z","timestamp":1790806252113,"version":"4.1.0"},"reference-count":83,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T00:00:00Z","timestamp":1781308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n de la Investigaci\u00f3n, el Desarrollo Tecnol\u00f3gico y la Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PICT2021-92"],"award-info":[{"award-number":["PICT2021-92"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"award":["PICT2021-92"],"award-info":[{"award-number":["PICT2021-92"]}],"id":[{"id":"https:\/\/ror.org\/03stxzb56","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Natinal University of Quilmes","award":["PUNQ 990\/19 and PUNQ 745\/25"],"award-info":[{"award-number":["PUNQ 990\/19 and PUNQ 745\/25"]}]},{"award":["PUNQ 990\/19 and PUNQ 745\/25"],"award-info":[{"award-number":["PUNQ 990\/19 and PUNQ 745\/25"]}],"id":[{"id":"https:\/\/ror.org\/01r53hz59","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The development of bio-based active packaging materials has gained increasing attention as a sustainable alternative to synthetic plastics. In this study, chitosan-based films incorporating yerba mate kombucha infusion (YMK-I) were developed and fully characterized. Films were prepared using different YMK-I concentrations (25\u2013100% v\/v) as solvent, with acetic acid-based chitosan films as controls. The infusion showed pH 2.5, titratable acidity of 3.5%, total solids of 6%, high phenolic content (1085 mg GAE\/L), and reducing sugars (18.3 g\/L). Acetic and lactic acids were identified by high-performance liquid chromatography (HPLC). Minimum Inhibitory Concentration (MIC) values ranged from 0.03 \u00b5g\/mL for Staphylococcus aureus to 0.3 \u00b5g\/mL for Escherichia coli and Pseudomonas aeruginosa. Rheological results indicated that YMK-I performed similarly to acetic acid as a solvent. Fourier Transformed Infrared with Attenuated Total Reflectance (FTIR-ATR) suggested interactions between chitosan and bioactive compounds. Thermal analyses showed that YMK-I acted as a plasticizer and introduced thermolabile components, altering glass transition and degradation behavior. Increasing YMK-I content reduced tensile strength and increased elongation, indicating greater flexibility, while water vapor permeability increased due to hydrophilic compounds. Films enriched with YMK-I exhibited high antioxidant activity (Radical Scavenging Activity &gt; 85%) and strong antimicrobial effects (&gt;98% inhibition) against E. coli and S. aureus. These results highlight the potential of chitosan\u2013kombucha films as multifunctional materials for specialized applications.<\/jats:p>","DOI":"10.3390\/ijms27125346","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T08:57:58Z","timestamp":1781513878000},"page":"5346","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Active Chitosan Films Enriched with Yerba Mate Kombucha Infusion: Formulation and Characterization"],"prefix":"10.3390","volume":"27","author":[{"given":"Celeste","family":"Cottet","sequence":"first","affiliation":[{"name":"Laboratory of Obtention, Modification, Characterization and Evaluation of Materials (LOMCEM), Department of Science and Technology, National University of Quilmes, Bernal B1876BXD, Argentina"},{"name":"National Scientific and Technical Research Council (CONICET), Buenos Aires C1033AAG, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela A.","family":"Kikot","sequence":"additional","affiliation":[{"name":"National Scientific and Technical Research Council (CONICET), Buenos Aires C1033AAG, Argentina"},{"name":"Biotechnological Materials Laboratory (LaMaBio), Department of Science and Technology, National University of Quilmes, Bernal B1876BXD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2243-5430","authenticated-orcid":false,"given":"Mat\u00edas L.","family":"Nobile","sequence":"additional","affiliation":[{"name":"National Scientific and Technical Research Council (CONICET), Buenos Aires C1033AAG, Argentina"},{"name":"Biotransformation and Nucleic Acids Laboratory (LaByQAN), Department of Science and Technology, National University of Quilmes, Bernal B1876BXD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0911-9225","authenticated-orcid":false,"given":"Marcela F.","family":"Almassio","sequence":"additional","affiliation":[{"name":"INQUISUR, Department of Chemistry, National University of the South (UNS)-CONICET, Bah\u00eda Blanca B8000FTN, Argentina"},{"name":"Commission for Scientific Research (CIC-PBA), Buenos Aires B1900TOL, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4020-1336","authenticated-orcid":false,"given":"Andr\u00e9s G.","family":"Salvay","sequence":"additional","affiliation":[{"name":"Laboratory of Obtention, Modification, Characterization and Evaluation of Materials (LOMCEM), Department of Science and Technology, National University of Quilmes, Bernal B1876BXD, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3152-8580","authenticated-orcid":false,"given":"Mercedes A.","family":"Peltzer","sequence":"additional","affiliation":[{"name":"Laboratory of Obtention, Modification, Characterization and Evaluation of Materials (LOMCEM), Department of Science and Technology, National University of Quilmes, Bernal B1876BXD, Argentina"},{"name":"National Scientific and Technical Research Council (CONICET), Buenos Aires C1033AAG, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1016\/j.msec.2013.01.010","article-title":"Chitosan based edible films and coatings: A review","volume":"33","author":"Elsabee","year":"2013","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"(1958). The British Food Journal Volume 60 Issue 6 1958. Br. Food J., 60, 57\u201368.","DOI":"10.1108\/eb011555"},{"key":"ref_3","first-page":"100598","article-title":"Plant-based chitosan for the development of biodegradable packaging materials","volume":"8","author":"Bahndral","year":"2024","journal-title":"Carbohydr. Polym. Technol. Appl."},{"key":"ref_4","unstructured":"Ebnesajjad, S. (2013). Handbook of Biopolymers and Biodegradable Plastics, William Andrew."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Guti\u00e9rrez, T.J. (2018). Polymers for food applications: News. Polymers for Food Applications, Springer.","DOI":"10.1007\/978-3-319-94625-2"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Jin, S.G. (2026). Recent Applications of Chitosan-Based Biomaterials as Wound Dressings. Int. J. Mol. Sci., 27.","DOI":"10.3390\/ijms27041637"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.carbpol.2017.07.053","article-title":"Bioadhesive and biocompatible films as wound dressing materials based on a novel dendronized chitosan loaded with ciprofloxacin","volume":"175","author":"Aldana","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.tifs.2008.07.003","article-title":"Biodegradable polymers for food packaging: A review","volume":"19","author":"Siracusa","year":"2008","journal-title":"Trends Food Sci. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.jclepro.2018.01.169","article-title":"Environmental impact of biodegradable food packaging when considering food waste","volume":"180","author":"Lane","year":"2018","journal-title":"J. Clean. Prod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.wasman.2016.10.006","article-title":"Biodegradation of bioplastics in natural environments","volume":"59","author":"Emadian","year":"2017","journal-title":"Waste Manag."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.ijbiomac.2019.07.089","article-title":"Impact of acid type for chitosan dissolution on the characteristics and biodegradability of cornstarch\/chitosan based films","volume":"138","author":"Pavoni","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e08674","DOI":"10.1016\/j.heliyon.2021.e08674","article-title":"Drug release study of the chitosan-based nanoparticles","volume":"8","author":"Herdiana","year":"2022","journal-title":"Heliyon"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Melro, E., Antunes, F.E., da Silva, G.J., Cruz, I., Ramos, P.E., Carvalho, F., and Alves, L. (2021). Chitosan films in food applications. Tuning film properties by changing acidic dissolution conditions. Polymers, 23.","DOI":"10.3390\/polym13010001"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Yan, D., Li, Y., Liu, Y., Li, N., Zhang, X., and Yan, C. (2021). Antimicrobial properties of chitosan and chitosan derivatives in the treatment of enteric infections. Molecules, 26.","DOI":"10.3390\/molecules26237136"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s12934-019-1083-3","article-title":"Engineering microbial consortia by division of labor","volume":"18","author":"Roell","year":"2019","journal-title":"Microb. Cell Fact."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1111\/1574-6976.12019","article-title":"Microbial syntrophy: Interaction for the common good","volume":"37","author":"Morris","year":"2013","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1146\/annurev-micro-090110-102844","article-title":"Genomic insights into syntrophy: The paradigm for anaerobic metabolic cooperation","volume":"66","author":"Sieber","year":"2012","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Kouzuma, A., Kato, S., and Watanabe, K. (2015). Microbial interspecies interactions: Recent findings in syntrophic consortia. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00477"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6712","DOI":"10.1038\/s41467-025-61957-x","article-title":"Microbial consortia for the conversion of biomass into fuels and chemicals","volume":"16","author":"Troiano","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00157-22","DOI":"10.1128\/msystems.00157-22","article-title":"Microbial Diversity and Interaction Specificity in Kombucha Tea Fermentations","volume":"7","author":"Landis","year":"2022","journal-title":"mSystems"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s13568-015-0124-5","article-title":"Metabarcoding of the kombucha microbial community grown in different microenvironments","volume":"5","author":"Reva","year":"2015","journal-title":"AMB Express"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Harrison, K., and Curtin, C. (2021). Microbial composition of scoby starter cultures used by commercial kombucha brewers in North America. Microorganisms, 9.","DOI":"10.3390\/microorganisms9051060"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"116634","DOI":"10.1016\/j.indcrop.2023.116634","article-title":"A new approach to obtain chitosan films\u2013Characteristics of films prepared with tea and coffee kombucha as natural chitosan solvents","volume":"197","author":"Stefanowska","year":"2023","journal-title":"Ind. Crops Prod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"18792","DOI":"10.1038\/s41598-021-98191-6","article-title":"Effect of fermentation time on the content of bioactive compounds with cosmetic and dermatological properties in Kombucha Yerba Mate extracts","volume":"11","author":"Ziemlewska","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wang, B., Rutherfurd-Markwick, K., Zhang, X.X., and Mutukumira, A.N. (2022). Kombucha: Production and Microbiological Research. Foods, 11.","DOI":"10.3390\/foods11213456"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1111\/1541-4337.12073","article-title":"A review on kombucha tea-microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus","volume":"13","author":"Jayabalan","year":"2014","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"R138","DOI":"10.1111\/j.1750-3841.2007.00535.x","article-title":"Yerba mate tea (Ilex paraguariensis): A comprehensive review on chemistry, health implications, and technological considerations","volume":"72","author":"Heck","year":"2007","journal-title":"J. Food Sci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Mihai, R.A., Cubi-Insuaste, N.S., and Catana, R.D. (2024). Biological Activity and Phenolic Content of Kombucha Beverages under the Influence of Different Tea Extract Substrates. Fermentation, 10.","DOI":"10.3390\/fermentation10070338"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"102346","DOI":"10.1016\/j.ifset.2020.102346","article-title":"Chitosan-based biodegradable functional films for food packaging applications","volume":"62","author":"Priyadarshi","year":"2020","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Wardejn, S., Wac\u0142awek, S., and Dudek, G. (2024). Improving Antimicrobial Properties of Biopolymer-Based Films in Food Packaging: Key Factors and Their Impact. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252312580"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"407","DOI":"10.31883\/pjfns\/112276","article-title":"Survival of wild strains of lactobacilli during Kombucha fermentation and their contribution to functional characteristics of beverage","volume":"69","author":"Pezo","year":"2019","journal-title":"Pol. J. Food Nutr. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Salinas-Ruiz, J.P., Guevara Garc\u00eda, J., Rios Tovar, D., Cruz Cabrera, R.P., and Torrico, D.D. (2026). Effects of Fermentation Time and Temperature on the Physicochemical Quality of Kombucha. Foods, 15.","DOI":"10.3390\/foods15071226"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Al-Mohammadi, A.R., Ismaiel, A.A., Ibrahim, R.A., Moustafa, A.H., Zeid, A.A., and Enan, G. (2021). Chemical constitution and antimicrobial activity of kombucha fermented beverage. Molecules, 26.","DOI":"10.3390\/molecules26165026"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"P\u0142atkiewicz, J., Oko\u0142owicz, D., Frankowski, R., Grze\u015bkowiak, T., Jeszka-Skowron, M., and Zgo\u0142a-Grze\u015bkowiak, A. (2024). Antioxidant Capacity, Phenolic Compounds, and Other Constituents of Cold and Hot Yerba Mate (Ilex paraguariensis) Infusions. Antioxidants, 13.","DOI":"10.3390\/antiox13121467"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"108782","DOI":"10.1016\/j.foodres.2019.108782","article-title":"de Kombuchas from green and black teas have different phenolic profile, which impacts their antioxidant capacities, antibacterial and antiproliferative activities","volume":"128","author":"Cardoso","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_36","first-page":"100310","article-title":"Kombucha fermentation in yerba mate: Cellulose production, films formulation and its characterisation","volume":"5","author":"Tapias","year":"2023","journal-title":"Carbohydr. Polym. Technol. Appl."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/j.foodchem.2006.05.032","article-title":"Changes in content of organic acids and tea polyphenols during kombucha tea fermentation","volume":"102","author":"Jayabalan","year":"2007","journal-title":"Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Zeinab, B., Buthaina, J., and Rafik, K. (2020). Resistance of gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules, 25.","DOI":"10.3390\/molecules25061340"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Rajput, P., Nahar, K.S., and Rahman, K.M. (2024). Evaluation of Antibiotic Resistance Mechanisms in Gram-Positive Bacteria. Antibiotics, 13.","DOI":"10.3390\/antibiotics13121197"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Tandhanskul, A., Krungkaew, S., Li, L., Kham, S.A., and Yonekura, L. (2025). Kombucha as a Sustainable Source of Metabiotics: Potential, Applications, and Future Perspectives. Beverages, 11.","DOI":"10.3390\/beverages11060173"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1111\/j.1750-3841.2011.02255.x","article-title":"Antimicrobial Activity of Yerba Mate (Ilex paraguariensis) Aqueous Extracts against Escherichia coli O157:H7 and Staphylococcus aureus","volume":"76","author":"Burris","year":"2011","journal-title":"J. Food Sci."},{"key":"ref_42","first-page":"93","article-title":"Actividad antimicrobiana de la yerba mate (Ilex paraguariensis St. Hil.) contra pat\u00f3genos alimentarios","volume":"45","author":"Porto","year":"2013","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Rachwa\u0142, K., and Gustaw, K. (2025). Plant-Derived Phytobiotics as Emerging Alternatives to Antibiotics Against Foodborne Pathogens. Appl. Sci., 15.","DOI":"10.3390\/app15126774"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Ji, Q.Y., Wang, W., Yan, H., Qu, H., Liu, Y., Qian, Y., and Gu, R. (2023). The Effect of Different Organic Acids and Their Combination on the Cell Barrier and Biofilm of Escherichia coli. Foods, 12.","DOI":"10.3390\/foods12163011"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.foodhyd.2012.11.028","article-title":"Structural properties of films and rheology of film-forming solutions based on chitosan and chitosan-starch blend enriched with murta leaf extract","volume":"31","author":"Bifani","year":"2013","journal-title":"Food Hydrocoll."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2748","DOI":"10.1007\/s10924-017-1166-3","article-title":"Influence of Glutaraldehyde Crosslinking and Alkaline Post-treatment on the Properties of Chitosan-Based Films","volume":"26","author":"Frick","year":"2018","journal-title":"J. Polym. Environ."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1002\/jrs.1238","article-title":"Infrared and Raman characteristic group frequencies. Tables and charts George Socrates John Wiley and Sons, Ltd., Chichester, Third Edition, 2001. Price \u00a3135","volume":"35","author":"Long","year":"2004","journal-title":"J. Raman Spectrosc."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"141","DOI":"10.3390\/md13010141","article-title":"Does the use of chitosan contribute to oxalate kidney stone formation?","volume":"13","author":"Queiroz","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.ijbiomac.2017.12.028","article-title":"Preparation and characterization of biocomposite film based on chitosan and kombucha tea as active food packaging","volume":"108","author":"Ashrafi","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Kaashyap, M., Cohen, M., and Kaashyap, N. (2021). Microbial Diversity and Characteristics of Kombucha as Revealed by Metagenomic and Physicochemical Analysis. Nutrients, 13.","DOI":"10.3390\/nu13124446"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1016\/j.foodchem.2012.09.081","article-title":"Preparation and functional properties of fish gelatin-chitosan blend edible films","volume":"136","author":"Rezaei","year":"2013","journal-title":"Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"109984","DOI":"10.1016\/j.lwt.2020.109984","article-title":"Structure and properties of chitosan films: Effect of the type of solvent acid","volume":"135","author":"Qiao","year":"2021","journal-title":"LWT"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Cottet, C., Fern\u00e1ndez-Garc\u00eda, M., and Peltzer, M.A. (2024). Evaluation of Different Concentrations of Antimicrobial Quaternary Polymers on the Behavior of Gelatin- and Starch-Based Films. Polymers, 16.","DOI":"10.3390\/polym16223168"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.carbpol.2016.09.018","article-title":"Characterization of nanocomposite film based on chitosan intercalated in clay platelets by electron beam irradiation","volume":"157","author":"Shahbazi","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.carbpol.2019.02.019","article-title":"Tailoring physicochemical properties of chitosan films and their protective effects on meat by varying drying temperature","volume":"212","author":"Liu","year":"2019","journal-title":"Carbohydr. Polym."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"47306","DOI":"10.1002\/app.47306","article-title":"A flame retarded chitosan binder for insulating miscanthus\/recycled textile fibers reinforced biocomposites","volume":"136","author":"Khalaf","year":"2019","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.polymdegradstab.2014.12.006","article-title":"Advanced physico-chemical characterization of chitosan by means of TGA coupled on-line with FTIR and GCMS: Thermal degradation and water adsorption capacity","volume":"112","author":"Corazzari","year":"2015","journal-title":"Polym. Degrad. Stab."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"6102","DOI":"10.3390\/ijms13056102","article-title":"Physiochemical, optical and biological activity of chitosan-chromone derivative for biomedical applications","volume":"13","author":"Kumar","year":"2012","journal-title":"Int. J. Mol. Sci."},{"key":"ref_59","first-page":"100232","article-title":"Physicochemical properties of chitosan edible films incorporated with different classes of flavonoids","volume":"4","author":"Sutharsan","year":"2022","journal-title":"Carbohydr. Polym. Technol. Appl."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"630","DOI":"10.29072\/basjs.20220308","article-title":"Preparation and Study of Some Physical and Mechanical Properties of Plasticized Chitosan Films","volume":"40","author":"Mutasher","year":"2022","journal-title":"Basra J. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.polymer.2016.02.031","article-title":"Designing green plasticizers: Influence of molecule geometry and alkyl chain length on the plasticizing effectiveness of diester plasticizers in PVC blends","volume":"89","author":"Erythropel","year":"2016","journal-title":"Polymer"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"102032","DOI":"10.1016\/j.bcab.2021.102032","article-title":"Kombucha beverage from non-conventional edible plant infusion and green tea: Characterization, toxicity, antioxidant activities and antimicrobial properties","volume":"34","author":"Silva","year":"2021","journal-title":"Biocatal. Agric. Biotechnol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"100265","DOI":"10.1016\/j.foohum.2024.100265","article-title":"The influence of material and color of food packaging on consumers\u2019 perception and consumption willingness","volume":"2","author":"Berthold","year":"2024","journal-title":"Food Humanit."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2976","DOI":"10.1007\/s10924-022-02412-6","article-title":"Water Vapour Transport in Biopolymeric Materials: Effects of Thickness and Water Vapour Pressure Gradient on Yeast Biomass-Based Films","volume":"30","author":"Delgado","year":"2022","journal-title":"J. Polym. Environ."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.eurpolymj.2017.11.051","article-title":"Hydration and water vapour transport properties in yeast biomass based films: A study of plasticizer content and thickness effects","volume":"99","author":"Delgado","year":"2018","journal-title":"Eur. Polym. J."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0926-6690(99)00032-1","article-title":"Water vapor transport parameters of a cast wheat gluten film","volume":"11","author":"Roy","year":"2000","journal-title":"Ind. Crops Prod."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1016\/j.jfoodeng.2006.07.016","article-title":"Water vapor permeability of edible starch based films","volume":"80","author":"Bertuzzi","year":"2007","journal-title":"J. Food Eng."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1111\/j.1365-2621.1993.tb06183.x","article-title":"Permeability and Mechanical Properties of Cellulose-Based Edible Films","volume":"58","author":"Park","year":"1993","journal-title":"J. Food Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1111\/j.1365-2621.1996.tb13163.x","article-title":"Functional properties of myofibrillar protein-based biopackaging as affected by film thickness","volume":"61","author":"Cuq","year":"1996","journal-title":"J. Food Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1111\/j.1365-2621.1993.tb09387.x","article-title":"Hydrophilic Edible Films: Modified Procedure for Water Vapor Permeability and Explanation of Thickness Effects","volume":"58","author":"McHugh","year":"1993","journal-title":"J. Food Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2403","DOI":"10.1021\/acs.jchemed.2c00449","article-title":"Measurement of the Water Vapor Permeability of Chitosan Films: A Laboratory Experiment on Food Packaging Materials","volume":"99","author":"Velazquez","year":"2022","journal-title":"J. Chem. Educ."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1111\/ijfs.12623","article-title":"Antioxidant activity and physicochemical properties of chitosan films incorporated with Lycium barbarum fruit extract for active food packaging","volume":"50","author":"Wang","year":"2014","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.tifs.2018.08.004","article-title":"Trends in Food Science & Technology Effect of plant extracts on the techno-functional properties of biodegradable packaging films","volume":"80","author":"Ahmad","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"5657","DOI":"10.1007\/s12649-024-02524-1","article-title":"Valorization of Wine Lees: Assessment of Antioxidant, Antimicrobial and Enzyme Inhibitory Activity of Wine Lees Extract and Incorporation in Chitosan Films","volume":"15","author":"Evangelos","year":"2024","journal-title":"Waste Biomass Valorization"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1016\/j.jfca.2011.01.008","article-title":"Journal of Food Composition and Analysis Comparison of ABTS\/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods \u00a7","volume":"24","author":"Floegel","year":"2011","journal-title":"J. Food Compos. Anal."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Khubiev, O.M., Egorov, A.R., Kirichuk, A.A., Khrustalev, V.N., Tskhovrebov, A.G., and Kritchenkov, A.S. (2023). Chitosan-Based Antibacterial Films for Biomedical and Food Applications. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241310738"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.lwt.2011.12.033","article-title":"Antimicrobial effect of Kombucha analogues","volume":"47","author":"Battikh","year":"2012","journal-title":"LWT"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"2781","DOI":"10.1007\/s11947-024-03629-0","article-title":"Yerba Mate and Water Kefir Grain Films for Food Preservation and Freshness Indicators","volume":"18","author":"Rezzani","year":"2025","journal-title":"Food Bioprocess Technol."},{"key":"ref_79","unstructured":"CLSI (2015). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, Clinical and Laboratory Standards Institute. [10th ed.]. CLSI document M07-A10."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Irgens, F. (2013). Rheology and Non-Newtonian Fluids, Springer.","DOI":"10.1007\/978-3-319-01053-3"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Ram\u00edrez Tapias, Y.A., Rezzani, G.D., Delgado, J.F., Peltzer, M.A., and Salvay, A.G. (2024). New Materials from the Integral Milk Kefir Grain Biomass and the Purified Kefiran: The Role of Glycerol Content on the Film\u2019s Properties. Polymers, 16.","DOI":"10.3390\/polym16223106"},{"key":"ref_82","unstructured":"(2013). Standard Test Methods for Water Vapor Transmission of Materials (Standard No. ASTM E96\/E96M)."},{"key":"ref_83","unstructured":"(2001). Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions (Withdrawn 2010) (Standard No. ASTM E2149-01). Available online: https:\/\/www.astm.org."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/12\/5346\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T04:25:28Z","timestamp":1781843128000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/12\/5346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,13]]},"references-count":83,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["ijms27125346"],"URL":"https:\/\/doi.org\/10.3390\/ijms27125346","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,13]]}}}