{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:15:08Z","timestamp":1785406508688,"version":"3.56.0"},"reference-count":31,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T00:00:00Z","timestamp":1781308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["DP1 DA048493"],"award-info":[{"award-number":["DP1 DA048493"]}]},{"name":"Manhattan HIV Brain Bank","award":["U24MH100931"],"award-info":[{"award-number":["U24MH100931"]}]},{"name":"Texas NeuroAIDS Research Center","award":["U24MH100930"],"award-info":[{"award-number":["U24MH100930"]}]},{"name":"National Neurological AIDS Bank","award":["U24MH100929"],"award-info":[{"award-number":["U24MH100929"]}]},{"award":["U24MH100929"],"award-info":[{"award-number":["U24MH100929"]}],"id":[{"id":"https:\/\/ror.org\/05ghs6f64","id-type":"ROR","asserted-by":"publisher"}]},{"name":"California NeuroAIDS Tissue Network","award":["U24MH100928"],"award-info":[{"award-number":["U24MH100928"]}]},{"name":"Data Coordinating Center","award":["U24MH100925"],"award-info":[{"award-number":["U24MH100925"]}]},{"award":["U24MH100925"],"award-info":[{"award-number":["U24MH100925"]}],"id":[{"id":"https:\/\/ror.org\/00fzvsb30","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The diversity and abundance of the brain virome is an active field of investigation. However, how the brain virome relates to the presence of viruses outside of the nervous system remains unclear. The rationale for this study is that analyses across multiple biologically linked compartments within the same individuals provide an important opportunity to evaluate virome discordance and viral burden. To characterize viral prevalence and burden across anatomical compartments, we applied the targeted viral enrichment method ViroFind to matched postmortem brain (n = 66), cerebrospinal fluid (CSF; n = 24), and peripheral samples (spleen, peripheral blood mononuclear cells, and lymph nodes; n = 66) from individuals with and without human immunodeficiency virus (HIV) infection and substance use disorder (SUD) in the National NeuroAIDS Tissue Consortium. We detected nucleic acids from 27 viruses representing 12 taxa. Several viruses, including adenovirus, torque teno virus, Epstein\u2013Barr virus, human herpesvirus 6 and 7, cytomegalovirus, parvovirus, and JC polyomavirus, showed significant inter-compartment differences in prevalence or burden. CSF exhibited lower overall viral diversity than brain or peripheral samples, whereas peripheral samples showed the highest viral burden. CNS viral detection was more likely when the same virus was also detected in the periphery. We also detected HBV and HCV in CNS samples despite them not being classically regarded as neurotropic. Broader virome profiling showed greater peripheral viral burden and diversity in HIV-positive than HIV-negative individuals, whereas SUD was not associated with overall viral burden differences. These findings highlight important cross-compartment differences in viral detection, including occurrence of occult HBV infection within the CNS, and support the value of CNS\u2013periphery comparisons in virome studies. These findings can contribute to improved diagnosis and management of viral infections.<\/jats:p>","DOI":"10.3390\/ijms27125349","type":"journal-article","created":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T08:57:58Z","timestamp":1781513878000},"page":"5349","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cross-Compartment Virome Profiling in Human Immunodeficiency Virus Infection and Substance Use Disorder Reveals Brain\u2013CSF\u2013Periphery Discordance and Hepatitis B Virus in Central Nervous System"],"prefix":"10.3390","volume":"27","author":[{"given":"Xin","family":"Dang","sequence":"first","affiliation":[{"name":"Davee Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1060-9448","authenticated-orcid":false,"given":"Barbara A.","family":"Hanson","sequence":"additional","affiliation":[{"name":"Davee Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Lopez","sequence":"additional","affiliation":[{"name":"Davee Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"Miller","sequence":"additional","affiliation":[{"name":"Northwestern Medicine, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6836-1468","authenticated-orcid":false,"given":"Igor J.","family":"Koralnik","sequence":"additional","affiliation":[{"name":"Davee Department of Neurology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ghose, C., Ly, M., Schwanemann, L.K., Shin, J.H., Atab, K., Barr, J.J., Little, M., Schooley, R.T., Chopyk, J., and Pride, D.T. 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