{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T04:48:28Z","timestamp":1782449308879,"version":"3.54.5"},"reference-count":39,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T00:00:00Z","timestamp":1781481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The global dissemination of carbapenem resistance is predominantly facilitated by plasmid-mediated carbapenemase genes, notably blaOXA-48-like genes. A comprehensive understanding of their evolutionary relationships and structural conservation is essential for monitoring their spread and informing therapeutic strategies. This study aimed to investigate the phylogenetic relationships and structural conservation of blaOXA-48-like carbapenemase genes in multiple Gram-negative bacterial species. We analysed blaOXA-48-like carbapenemase sequences obtained from a hospital in Pakistan and compared them with globally reported variants retrieved from GenBank. Carbapenemase gene sequences (blaOXA-48-like, blaNDM, and blaVIM) were analyzed using maximum-likelihood phylogenetics (MEGA11, Tamura\u2013Nei model, 1000 bootstrap replicates). Comparative global sequences were retrieved from GenBank. Structural modeling of blaOXA-48-like genes was performed using SWISS-MODEL Workspace with the template PDB 3HBR, followed by validation using GMQE, QMEANDisCo, and Ramachandran plot analyses. Phylogenetic analysis revealed a tight clustering of blaOXA-48-like genes across A. baumannii, K. pneumoniae, and E. meningoseptica, showing high similarity to globally distributed plasmid-associated sequences. Structural modeling demonstrated strong conservation of the enzyme, with preserved catalytic residues (Ser70, Lys73, Ser118, Trp157, and Tyr211) and minimal structural deviation (RMSD &lt; 0.3 \u00c5). blaOXA-48-like carbapenemases exhibit strong phylogenetic conservation and structural stability across species and regions, consistent with the horizontal dissemination of blaOXA-48-like genes across bacterial hosts. These findings indicate that blaOXA-48-like carbapenemases have high evolutionary stability.<\/jats:p>","DOI":"10.3390\/ijms27125391","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:49:01Z","timestamp":1781570941000},"page":"5391","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phylogenetic Relationships and Structural Conservation of blaOXA-48-like Carbapenemase in Multispecies Clinical Strains from an Intensive Care Unit in Pakistan"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9486-3701","authenticated-orcid":false,"given":"Zeb","family":"Hussain","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Sciences, Dow Institute of Medical Technology, Dow University of Health Sciences, Karachi 75280, Pakistan"},{"name":"Department of Microbiology, University of Karachi, Karachi 75270, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5653-2012","authenticated-orcid":false,"given":"Ambreen","family":"Fatima","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dow International Medical College, Dow University of Health Sciences, Karachi 75280, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asad","family":"Karim","sequence":"additional","affiliation":[{"name":"Jamil-UR-Rehman Centre for Genome Research, Dr. Panjwani Centre for Molecular Medicine & Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Jahanzaib","sequence":"additional","affiliation":[{"name":"Jamil-UR-Rehman Centre for Genome Research, Dr. Panjwani Centre for Molecular Medicine & Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Sameer","family":"Qureshi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Sciences, Dow Institute of Medical Technology, Dow University of Health Sciences, Karachi 75280, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0571-0189","authenticated-orcid":false,"given":"Asma","family":"Naim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Karachi, Karachi 75270, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e955","DOI":"10.1016\/S2468-2667(25)00201-4","article-title":"The burden of bacterial antimicrobial resistance in the WHO Eastern Mediterranean Region 1990\u20132021: A cross-country systematic analysis with forecasts to 2050","volume":"10","author":"Mestrovic","year":"2025","journal-title":"Lancet Public Health"},{"key":"ref_2","unstructured":"World Health Organization (2025). 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