{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:47:27Z","timestamp":1785422847417,"version":"3.56.0"},"reference-count":54,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["23-74-10105"],"award-info":[{"award-number":["23-74-10105"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]},{"award":["23-74-10105"],"award-info":[{"award-number":["23-74-10105"]}],"id":[{"id":"https:\/\/ror.org\/03y2gwe85","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Ministry of Education and Science","award":["125041005114-8"],"award-info":[{"award-number":["125041005114-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Multidrug resistance (MDR) is a global problem for the healthcare system, complicating the therapy of bacterial infections. It is noted that patients infected with MDR strains often require prolonged hospitalization, have a high risk of mortality, and remain vulnerable to reinfection after recovery. In this study, recombinant phage-encoded depolymerase DepKP144 was produced using the Escherichia coli expression system and purified. The depolymerase DepKP144 protein was able to reduce viable bacterial counts following capsule degradation in 95% of the tested strains of type K1 and 85% of the tested strains of type K2 Klebsiella pneumoniae. The depolymerase DepKP144 was active against K. pneumoniae K1-type and K2-type planktonic cells and destroyed the biofilms formed by clinical MDR strains of K. pneumoniae. In in vivo experiments, DepKP144 at a dose of 180 \u03bcg\/mouse resulted in a 50% survival of the mice infected with K2-type K. pneumoniae and in a 17% survival of the mice infected with K1-type K. pneumoniae. This depolymerase is promising for further development of prevention and therapeutic candidates against MDR K. pneumoniae.<\/jats:p>","DOI":"10.3390\/ijms27125466","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T10:19:53Z","timestamp":1781691593000},"page":"5466","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phage-Encoded Depolymerase DepKP144 with Therapeutic Potential Against Both K1- and K2-Type Klebsiella pneumoniae"],"prefix":"10.3390","volume":"27","author":[{"given":"Ekaterina A.","family":"Kondakova","sequence":"first","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia N.","family":"Golosova","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2066-8975","authenticated-orcid":false,"given":"Bogdana I.","family":"Kravchuk","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1413-5876","authenticated-orcid":false,"given":"Yana A.","family":"Khlusevich","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vyacheslav I.","family":"Yakubovskij","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuliya N.","family":"Kozlova","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana A.","family":"Grishkova","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"},{"name":"Faculty of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina V.","family":"Tikunova","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6884-6104","authenticated-orcid":false,"given":"Andrey L.","family":"Matveev","sequence":"additional","affiliation":[{"name":"Institute of Chemical Biology and Fundamental Medicine Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6549","DOI":"10.2147\/IDR.S422564","article-title":"Establishment and Validation of Models for the Risk of Multi-Drug Resistant Bacteria Infection and Prognosis in Elderly Patients with Pulmonary Infection: A Multicenter Retrospective Study","volume":"16","author":"Wang","year":"2023","journal-title":"Infect. 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