{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:36:12Z","timestamp":1785418572492,"version":"3.56.0"},"reference-count":28,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32371542"],"award-info":[{"award-number":["32371542"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32371542"],"award-info":[{"award-number":["32371542"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Zhejiang Provincial Natural Science Foundation of China","doi-asserted-by":"publisher","award":["LY23B060001"],"award-info":[{"award-number":["LY23B060001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"award":["LY23B060001"],"award-info":[{"award-number":["LY23B060001"]}],"id":[{"id":"https:\/\/ror.org\/04aqat463","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008137","name":"Ministry of Natural Resources","doi-asserted-by":"publisher","award":["HY202305"],"award-info":[{"award-number":["HY202305"]}],"id":[{"id":"10.13039\/100008137","id-type":"DOI","asserted-by":"publisher"}]},{"award":["HY202305"],"award-info":[{"award-number":["HY202305"]}],"id":[{"id":"https:\/\/ror.org\/02kxqx159","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Tianjin Institute of Industrial Biotechnology","award":["(No. 2021KF003)"],"award-info":[{"award-number":["(No. 2021KF003)"]}]},{"award":["(No. 2021KF003)"],"award-info":[{"award-number":["(No. 2021KF003)"]}],"id":[{"id":"https:\/\/ror.org\/042pyga86","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Type XVII collagen (COL17) is crucial for skin integrity but difficult to produce. To achieve high-level secretory expression, a human COL17 segment was designed and cloned into the pPIC9K vector with six different \u03b1-mating factor signal peptides and integrated into Komagataella phaffii GS115. Fed-batch fermentation in a 5 L bioreactor yielded 4.7 g\/L of recombinant COL17. Functional assays showed that it promoted the proliferation of human skin fibroblast (HFF) and immortalized keratinocytes (HaCaT), upregulated COL1A1, COL3A1, and TIMP1 in HFF cells, and enhanced skin barrier-related genes (KRT1, KRT5, KRT10, KRT14, IVL, LOR, FLG) in HaCaT cells. In a UVB-induced photoaging model, COL17 reduced reactive oxygen species (ROS) and matrix metalloproteinase 3 (MMP3) activity. This recombinant collagen exhibits photoprotective, regenerative, and barrier-enhancing activities, offering potential for skincare and tissue engineering.<\/jats:p>","DOI":"10.3390\/ijms27125613","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T03:09:17Z","timestamp":1782270557000},"page":"5613","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-Level Secretory Expression of Recombinant Type XVII Human-like Collagen in Komagataella phaffii"],"prefix":"10.3390","volume":"27","author":[{"given":"Yixuan","family":"Jia","sequence":"first","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhao","family":"Yue","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanting","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weirui","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Ningbo Tech University, Ningbo 315100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6467-4262","authenticated-orcid":false,"given":"Sheng","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Ningbo Tech University, Ningbo 315100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lehe","family":"Mei","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Ningbo Tech University, Ningbo 315100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peilian","family":"Wei","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changjiang","family":"Lyu","sequence":"additional","affiliation":[{"name":"School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1007\/s42452-025-07780-x","article-title":"Collagen and collagen-derived materials: Synthesis, structure, classification, fundamental properties and biomedical applications","volume":"7","author":"Emam","year":"2025","journal-title":"Discov. 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