{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T03:49:46Z","timestamp":1782272986176,"version":"3.54.5"},"reference-count":81,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Dental caries is a biofilm-mediated dysbiotic disease characterized by ecological shifts within the oral microbiome and progressive demineralization of dental hard tissues. The microbiological composition of carious dentin and the impact of minimally invasive excavation techniques on residual microbial communities remain subjects of ongoing investigation due to methodological heterogeneity and inconsistencies among published studies. This narrative review aimed to summarize current evidence regarding the microbial ecology of carious dentin, compare culture-based and molecular methods for microbiological assessment, and evaluate the microbiological outcomes associated with contemporary approaches to managing minimally invasive caries. The relevant literature on dentinal caries microbiology, microbial detection methods, and excavation techniques was analyzed. The available evidence indicates that carious dentin contains a highly diverse polymicrobial community composed of acidogenic, aciduric, anaerobic, and proteolytic microorganisms. Culture-based methods primarily detect viable and cultivable taxa, whereas molecular approaches reveal substantially greater microbial diversity, including uncultivable and low-abundance species. Comparative studies demonstrate that minimally invasive excavation techniques significantly reduce microbial load but rarely achieve complete microbial elimination. The available evidence suggests that successful caries management is associated with a reduction in and ecological modulation of the residual microbiota within a sealed environment. The integration of culture-based and molecular findings provides a more comprehensive understanding of the microbiology of carious dentin and supports biologically oriented, minimally invasive strategies for caries management.<\/jats:p>","DOI":"10.3390\/ijms27135648","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T03:09:17Z","timestamp":1782270557000},"page":"5648","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial Composition of Carious Dentin and the Impact of Minimally Invasive Excavation Techniques: A Narrative Review"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4716-7724","authenticated-orcid":false,"given":"Nadezhda","family":"Mitova","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University\u2014Sofia, 1 Georgi Sofiyski St., 1431 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0116-259X","authenticated-orcid":false,"given":"Zornitsa","family":"Lazarova","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, Faculty of Dental Medicine, Medical University\u2014Sofia, 1 Georgi Sofiyski St., 1431 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Zhang, J.S., Chu, C.H., and Yu, O.Y. (2022). Oral Microbiome and Dental Caries Development. Dent. J., 10.","DOI":"10.3390\/dj10100184"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Marsh, P.D. (2006). Dental plaque as a biofilm and a microbial community\u2013implications for health and disease. BMC Oral Health, 6.","DOI":"10.1186\/1472-6831-6-S1-S14"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/s41579-023-00963-6","article-title":"The oral microbiome: Diversity, biogeography and human health","volume":"22","author":"Baker","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"106070","DOI":"10.1016\/j.archoralbio.2024.106070","article-title":"Oral microbiota: Taxonomic composition and functional profile in caries-free and in caries-affected individuals\u2014A systematic review","volume":"168","author":"Pagnussatti","year":"2024","journal-title":"Arch. Oral Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1016\/j.joen.2021.08.018","article-title":"Active Bacteria in Carious Dentin of Mandibular Molars with Different Pulp Conditions: An In Vivo Study","volume":"47","author":"Selvakumar","year":"2021","journal-title":"J. Endod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1177\/154405910408301s07","article-title":"What constitutes dental caries? Histopathology of carious enamel and dentin related to the action of cariogenic biofilms","volume":"83","author":"Kidd","year":"2004","journal-title":"J. Dent. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1038\/s41415-023-5515-4","article-title":"Minimally invasive selective caries removal: A clinical guide","volume":"234","author":"Lim","year":"2023","journal-title":"Br. Dent. J."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Barbosa, C.B., Monici Silva, I., Cena, J.A., Stefani, C.M., and Dame-Teixeira, N. (2023). Presence of host and bacterial-derived collagenolytic proteases in carious dentin: A systematic review. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1278754"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105468","DOI":"10.1016\/j.jdent.2024.105468","article-title":"Remineralisation of mineral-deficient dentine induced by experimental ion-releasing materials in combination with a biomimetic dual-analogue primer","volume":"152","author":"Chou","year":"2025","journal-title":"J. Dent."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"30712","DOI":"10.1038\/s41598-025-13977-2","article-title":"Elemental and micromorphological analysis of ion releasing restoration\/carious dentin interface","volume":"15","author":"Abdelsalam","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1159\/000070446","article-title":"Cultivatable bacteria in dentine after caries excavation using rose-bur or carisolv","volume":"37","author":"Lager","year":"2003","journal-title":"Caries Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ferreira, L.A.Q., Diniz, I.M.A., Peixoto, R.T.R.C., Gomes, N.A., Caneschi, C.d.S., Spineli, L.M., and Martins, C.C. (2023). Efficacy of antiseptics and chemomechanical methods for dentin caries lesions: Systematic review. Front. Oral Health, 4.","DOI":"10.3389\/froh.2023.1110634"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1177\/0022034516639276","article-title":"Managing Carious Lesions: Consensus Recommendations on Terminology","volume":"28","author":"Innes","year":"2016","journal-title":"Adv. Dent. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"30","DOI":"10.4103\/1735-3327.174695","article-title":"Microbiological analysis after complete or partial removal of carious dentin using two different techniques in primary teeth: A randomized clinical trial","volume":"13","author":"Singhal","year":"2016","journal-title":"Dent. Res. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e038638","DOI":"10.1136\/bmjopen-2020-038638","article-title":"Lasers to prevent dental caries: A systematic review","volume":"10","author":"Pagano","year":"2020","journal-title":"BMJ Open"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1159\/000447309","article-title":"Ecological Hypothesis of Dentin and Root Caries","volume":"50","author":"Takahashi","year":"2016","journal-title":"Caries Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.tim.2014.10.010","article-title":"Solving the etiology of dental caries","volume":"23","author":"Mira","year":"2015","journal-title":"Trends Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.phrs.2012.11.006","article-title":"The oral microbiome in health and disease","volume":"69","author":"Wade","year":"2013","journal-title":"Pharmacol. Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The human oral microbiome","volume":"192","author":"Dewhirst","year":"2020","journal-title":"J. Bacteriol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Chen, J., Kong, L., Peng, X., Chen, Y., Ren, B., Li, M., Li, J., Zhou, X., and Cheng, L. (2021). Core Microbiota Promotes the Development of Dental Caries. Appl. Sci., 11.","DOI":"10.3390\/app11083638"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1128\/JCM.01410-07","article-title":"Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults","volume":"46","author":"Aas","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3023","DOI":"10.1128\/JCM.42.7.3023-3029.2004","article-title":"Molecular Analysis of the Microflora Associated with Dental Caries","volume":"42","author":"Munson","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1177\/0022034517736496","article-title":"The Caries Microbiome: Implications for Reversing Dysbiosis","volume":"29","author":"Tanner","year":"2018","journal-title":"Adv. Dent. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1177\/0022034520913248","article-title":"Structural and Functional Characteristics of the Microbiome in Deep-Dentin Caries","volume":"99","author":"Liu","year":"2020","journal-title":"J. Dent. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1111\/iej.13128","article-title":"Management of deep caries and the exposed pulp","volume":"52","author":"Simon","year":"2019","journal-title":"Int. Endod. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1177\/0022034513477425","article-title":"Incomplete caries removal: A systematic review and meta-analysis","volume":"92","author":"Schwendicke","year":"2013","journal-title":"J. Dent. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"fuac052","DOI":"10.1093\/femsre\/fuac052","article-title":"Illuminating the oral microbiome and its host interactions: Tools and approaches for molecular ecological studies","volume":"47","author":"Kreth","year":"2023","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"103430","DOI":"10.1016\/j.jdent.2020.103430","article-title":"Pulp and dentine responses to selective caries excavation: A histological and histobacteriological human study","volume":"100","author":"Ricucci","year":"2020","journal-title":"J. Dent."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Spatafora, G., Li, Y., He, X., Cowan, A., and Tanner, A.C.R. (2024). The Evolving Microbiome of Dental Caries. Microorganisms, 12.","DOI":"10.3390\/microorganisms12010121"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105933","DOI":"10.1016\/j.archoralbio.2024.105933","article-title":"Oral microbiota of adolescents with dental caries: A systematic review","volume":"161","author":"Veenman","year":"2024","journal-title":"Arch. Oral Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1034\/j.1399-302X.2003.00051.x","article-title":"Mutans streptococci strains prevalence before and after cavity preparation during Atraumatic Restorative Treatment","volume":"18","author":"Toi","year":"2003","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Lazarova, Z., Gergova, R., and Mitova, N. (2025). Fluorescence as a quantitative indicator of Cariogenic Bacteria During Chemo-Mechanical Caries Excavation with BRIX 3000 in Primary Teeth. J. Funct. Biomater., 16.","DOI":"10.3390\/jfb16120453"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Lazarova, Z., Gergova, R., and Mitova, N. (2026). Photodynamic therapy as adjunct to BRIX 3000. Pharmaceuticals, 19.","DOI":"10.3390\/ph19020310"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Mitova, N., Gergova, R., Boyanov, V., Alexandrova, A., Karova, E., Grancharova, N., Dogandzhiyska, V., Mihaylova, Z., Marinova-Takorova, M., and Hristov, K. (2026). Detection of cariogenic bacteria: PCR vs culture. Appl. Sci., 16.","DOI":"10.3390\/app16083913"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1600-0528.1998.tb01938.x","article-title":"A practice-based study on stepwise excavation of deep carious lesions in permanent teeth: A 1-year follow-up study","volume":"26","author":"Thylstrup","year":"1998","journal-title":"Community Dent. Oral Epidemiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Fine, D.H., and Schreiner, H. (2023). Oral microbial interactions from an ecological perspective: A narrative review. Front. Oral Health, 4.","DOI":"10.3389\/froh.2023.1229118"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2198433","DOI":"10.1080\/20002297.2023.2198433","article-title":"Dental caries and their microbiomes in children: What do we do now?","volume":"15","author":"Ribeiro","year":"2023","journal-title":"J. Oral Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Jeong, J., Mun, S., Oh, Y., Cho, C.-S., Yun, K., Ahn, Y., Chung, W.-H., Lim, M.Y., Lee, K.E., and Hwang, T.S. (2022). A qRT-PCR Method Capable of Quantifying Specific Microorganisms Compared to NGS-Based Metagenome Profiling Data. Microorganisms, 10.","DOI":"10.3390\/microorganisms10020324"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1128\/JCM.40.5.1698-1704.2002","article-title":"Quantitative microbiological study of human carious dentine by culture and real-time PCR: Association of anaerobes with histopathological changes in chronic pulpitis","volume":"40","author":"Martin","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Obata, J., Takeshita, T., Shibata, Y., Yamanaka, W., Unemori, M., Akamine, A., and Yamashita, Y. (2014). Identification of the microbiota in carious dentin lesions using 16S rRNA gene sequencing. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0103712"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1111\/iej.13890","article-title":"Prediction and validation of microbial community function from normal pulp to pulpitis caused by deep dentinal caries","volume":"56","author":"Liu","year":"2023","journal-title":"Int. Endod. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1002\/cre2.464","article-title":"Microbiological testing of clinical samples before and after periodontal treatment. A comparative methodological study between real-time PCR and real-time-PCR associated to propidium monoazide","volume":"7","author":"Sereti","year":"2021","journal-title":"Clin. Exp. Dent. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"100281","DOI":"10.1016\/j.bioflm.2025.100281","article-title":"PMA-qPCR to quantify viable cells in multispecies oral biofilm after disinfectant treatments","volume":"9","author":"Schwendener","year":"2025","journal-title":"Biofilm"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41368-025-00421-4","article-title":"DNA and RNA-based amplicon sequencing of paired supragingival and dentin lesion plaque in children with severe early childhood caries","volume":"18","author":"Naumann","year":"2026","journal-title":"Int. J. Oral Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1016\/j.csbj.2021.02.010","article-title":"The oralome and its dysbiosis: New insights into oral microbiome-host interactions","volume":"19","author":"Radaic","year":"2021","journal-title":"Comput. Struct. Biotechnol. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1101\/gr.265645.120","article-title":"Deep metagenomics examines the oral microbiome during dental caries, revealing novel taxa and co-occurrences with host molecules","volume":"31","author":"Baker","year":"2021","journal-title":"Genome Res."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Rajasekaran, J.J., Krishnamurthy, H.K., Bosco, J., Jayaraman, V., Krishna, K., Wang, T., and Bei, K. (2024). Oral Microbiome: A Review of Its Impact on Oral and Systemic Health. Microorganisms, 12.","DOI":"10.20944\/preprints202406.1923.v1"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Mahdizade Ari, M., Scholz, K.J., Cieplik, F., and Al-Ahmad, A. (2025). Viable but non-cultivable state in oral microbiota: A critical review of an underexplored microbial survival strategy. Front. Cell. Infect. Microbiol., 15.","DOI":"10.3389\/fcimb.2025.1533768"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Rudkj\u00f8bing, V.B., Thomsen, T.R., Xu, Y., Melton-Kreft, R., Ahmed, A., Eickhardt, S., Bjarnsholt, T., Poulsen, S.S., Nielsen, P.H., and Earl, J.P. (2016). Comparing culture and molecular methods for the identification of microorganisms involved in necrotizing soft tissue infections. BMC Infect. Dis., 16.","DOI":"10.1186\/s12879-016-1976-2"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Desai, H., Stewart, C.A., and Finer, Y. (2021). Minimally Invasive Therapies for the Management of Dental Caries\u2014A Literature Review. Dent. J., 9.","DOI":"10.3390\/dj9120147"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jds.2022.07.021","article-title":"Selective versus stepwise removal of deep carious lesions: A meta-analysis of randomized controlled trials","volume":"18","author":"Yao","year":"2023","journal-title":"J. Dent. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1177\/00220345241303880","article-title":"Streptococcus mutans and Caries: A Systematic Review and Meta-Analysis","volume":"104","author":"Mazurel","year":"2025","journal-title":"J. Dent. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.sdentj.2023.01.010","article-title":"Chemomechanical caries removal methods: A literature review","volume":"35","author":"Maashi","year":"2023","journal-title":"Saudi Dent. J."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Kitsahawong, K., Kaewpitak, A., Rattanarangsima, K., and Leethongdee, S. (2024). Efficacy of chemo-mechanical caries removal: A 24-month follow-up systematic review and meta-analysis. Front. Oral Health, 5.","DOI":"10.3389\/froh.2024.1458530"},{"key":"ref_55","first-page":"444","article-title":"Evaluation of the Efficacy of Caries Removal Using Papain Gel (Brix 3000) and Smart Preparation Bur (in vivo Comparative Study)","volume":"11","author":"Ismail","year":"2019","journal-title":"J. Pharm. Sci. Res."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Lazarova, Z., and Mitova, N. (2026). Clinical and Biological Evaluation of Chemo-Mechanical Caries Excavation with Brix 3000 in Primary Molars: An 18-Month Prospective Study. Medicina, 62.","DOI":"10.3390\/medicina62040615"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"43","DOI":"10.22271\/oral.2025.v11.i1a.2101","article-title":"Minimally invasive pediatric dentistry: Management of carious lesions. A review","volume":"11","year":"2025","journal-title":"Int. J. Appl. Dent. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"109","DOI":"10.17796\/jcpd.39.2.n25754863557k727","article-title":"Efficacy and tolerance of papain gel with conventional drilling method: A clinico-microbiological study","volume":"39","author":"Goyal","year":"2015","journal-title":"J. Clin. Pediatr. Dent."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1590\/S1678-77572005000400016","article-title":"Comparison of the efficacy of chemomechanical and mechanical methods of caries removal in the reduction of S. mutans and Lactobacillus spp. in carious dentine of primary teeth","volume":"13","author":"Lima","year":"2005","journal-title":"J. Appl. Oral Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.14219\/jada.archive.2006.0111","article-title":"Chemomechanical caries removal in children: Efficacy and efficiency","volume":"137","author":"Peters","year":"2006","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Sachelarie, L., Cristea, R., Burlui, E., and Hurjui, L.L. (2024). Laser Technology in Dentistry: From Clinical Applications to Future Innovations. Dent. J., 12.","DOI":"10.3390\/dj12120420"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Fiegler-Rudol, J., Skaba, D., Kawczyk-Krupka, A., and Wiench, R. (2025). Antibacterial and Bactericidal Effects of the Er: YAG Laser on Oral Bacteria: A Systematic Review of Microbiological Evidence. J. Funct. Biomater., 16.","DOI":"10.3390\/jfb16060209"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Valenti, C., Pagano, S., Bozza, S., Ciurnella, E., Lomurno, G., Capobianco, B., Coniglio, M., Cianetti, S., and Marinucci, L. (2021). Use of the Er:YAG Laser in Conservative Dentistry: Evaluation of the Microbial Population in Carious Lesions. Materials, 14.","DOI":"10.3390\/ma14092387"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1007\/s10103-025-04624-3","article-title":"A comprehensive review on advancements in laser-assisted dental treatments: Focus on minimally invasive techniques and clinical outcomes","volume":"40","author":"Safaee","year":"2025","journal-title":"Lasers Med. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"106264","DOI":"10.1016\/j.jdent.2025.106264","article-title":"Efficacy of Er:YAG, Er,Cr:YSGG, and Nd:YAG lasers for caries removal: A systematic review","volume":"164","year":"2026","journal-title":"J. Dent."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0010-8545(02)00034-6","article-title":"Photosensitized singlet oxygen and its applications","volume":"233","author":"DeRosa","year":"2002","journal-title":"Coord. Chem. Rev."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.mib.2016.06.008","article-title":"Antimicrobial photodynamic inactivation: A bright new technique to kill resistant microbes","volume":"33","author":"Hamblin","year":"2016","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1177\/154405910708600803","article-title":"Photodynamic therapy in dentistry","volume":"86","author":"Konopka","year":"2007","journal-title":"J. Dent. Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1089\/pho.2012.3341","article-title":"Effects of photodynamic therapy on Gram-positive and Gram-negative bacterial biofilms by bioluminescence imaging and scanning electron microscopic analysis","volume":"31","author":"Garcez","year":"2013","journal-title":"Photomed. Laser Surg."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.pdpdt.2016.08.002","article-title":"Effect of photodynamic therapy with two photosensitizers onStreptococcus mutans: An in vitro study","volume":"16","author":"Azizi","year":"2016","journal-title":"Photodiagn. Photodyn. Ther."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"de Oliveira, A.B., Ferrisse, T.M., Marques, R.S., de Annunzio, S.R., Brighenti, F.L., and Fontana, C.R. (2019). Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20143585"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"167","DOI":"10.4103\/0970-4388.73793","article-title":"Dentin comparison in primary and permanent molars under transmitted and polarised light microscopy: An in vitro study","volume":"28","author":"Chowdhary","year":"2010","journal-title":"J. Indian Soc. Pedod. Prev. Dent."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"4431399","DOI":"10.1155\/tswj\/4431399","article-title":"Comparative Evaluation of Deciduous and Permanent Coronal Caries Using Polarizing Light Microscopy and Scanning Electron Microscopy","volume":"2025","author":"Thomas","year":"2025","journal-title":"Sci. World J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"188","DOI":"10.5005\/jp-journals-10005-1509","article-title":"Evaluation of dentin\u2013pulp complex response after conservative clinical procedures in primary teeth","volume":"11","author":"Mello","year":"2018","journal-title":"Int. J. Clin. Pediatr. Dent."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"279","DOI":"10.5114\/jos.2023.133652","article-title":"Clinical dilemma of selective caries removal in primary teeth: A scoping review","volume":"76","author":"Asturini","year":"2023","journal-title":"J. Stomatol."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Cherkaoui, A., and Schrenzel, J. (2022). Total Laboratory Automation for Rapid Detection and Identification of Microorganisms and Their Antimicrobial Resistance Profiles. Front. Cell. Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.807668"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Gheorghiu, I.M., Ciobanu, S., Roman, I., P\u0103unic\u0103, S., Dumitriu, A.S., and Iliescu, A.A. (2025). Deep Caries Lesions Revisited: A Narrative Review. J. Mind Med. Sci., 12.","DOI":"10.3390\/jmms12010037"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"214","DOI":"10.5005\/jp-journals-10005-1366","article-title":"A Comparative Evaluation of the Efficacy of Different Caries Excavation Techniques in reducing the Cariogenic Flora: An in vivo Study","volume":"9","author":"Hassan","year":"2016","journal-title":"Int. J. Clin. Pediatr. Dent."},{"key":"ref_79","first-page":"e31422","article-title":"Clinical and Microbiological Evaluation of the Chemomechanical Caries Removal Agents in Primary Molars","volume":"12","author":"Choudhary","year":"2022","journal-title":"Cureus"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1159\/000545052","article-title":"Effectiveness of Stepwise Excavation or Selective Excavation in Comparison with Non-Selective Caries Removal in Managing Deep Caries in Vital Permanent Teeth: A Systematic Review with Trial Sequential, Pairwise, and Network Meta-Analyses","volume":"60","author":"Ramezanzade","year":"2026","journal-title":"Caries Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/idj.12074","article-title":"The primary and mixed dentition, post-eruptive enamel maturation and dental caries: A review","volume":"63","author":"Lynch","year":"2013","journal-title":"Int. Dent. J."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5648\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T03:41:43Z","timestamp":1782272503000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,23]]},"references-count":81,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ijms27135648"],"URL":"https:\/\/doi.org\/10.3390\/ijms27135648","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,23]]}}}