{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T04:45:33Z","timestamp":1782621933095,"version":"3.54.5"},"reference-count":58,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007917","name":"USDA-ARS","doi-asserted-by":"crossref","award":["8042-22000-324-000-D"],"award-info":[{"award-number":["8042-22000-324-000-D"]}],"id":[{"id":"10.13039\/100007917","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Seashore paspalum (Paspalum vaginatum), a salinity-tolerant turfgrass, lacks well-characterized viral profiles. This study reports the discovery of a novel virus, tentatively named Paspalum latent virus (PaLV), representing a new taxon within the Alphaflexiviridae. Using high-throughput sequencing and RACE PCR, the 6995 nt genome was determined, revealing five open reading frames. Notably, PaLV lacks the AlkB domain and exhibits unique features, including overlapping start-stop codons (ORF4\/ORF5) and a second in-frame AUG in the coat protein (CP) region. Phylogenetic analysis of the replicase placed PaLV in a distinct clade, separate from Potexvirus and Lolavirus. Despite low sequence identity, AlphaFold2 revealed conserved CP structural domains. Genetic analysis of 11 isolates showed low diversity and strong purifying selection. Pathogenicity assays through mechanical transmission demonstrated a broad but latent host range, including Zea mays and Sorghum spp. These findings suggest PaLV represents a novel species within a putatively new genus, Paspalovirus. Given its 90% incidence rate and latent profile, the RT-PCR assay developed here is vital for routine molecular diagnostics in turfgrass management and germplasm conservation.<\/jats:p>","DOI":"10.3390\/ijms27135760","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T05:52:11Z","timestamp":1782453131000},"page":"5760","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular and Biological Characterization of a Newly Identified Virus Representing a Novel Taxon of Alphaflexiviridae Infecting Different Accessions of Seashore Paspalum, a Turfgrass, Widely Grown in the United States"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4610-3334","authenticated-orcid":false,"given":"Sayanta","family":"Bera","sequence":"first","affiliation":[{"name":"United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program (PGQP), Beltsville, MD 20708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor F.","family":"Schulden","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program (PGQP), Beltsville, MD 20708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4464-206X","authenticated-orcid":false,"given":"Xiaojun","family":"Hu","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program (PGQP), Beltsville, MD 20708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9613-2838","authenticated-orcid":false,"given":"Peter","family":"Abrahamian","sequence":"additional","affiliation":[{"name":"USDA-ARS National Germplasm Resources Laboratory, Beltsville, MD 20705, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yang","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program (PGQP), Beltsville, MD 20708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna L.","family":"Paulson","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program (PGQP), Beltsville, MD 20708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Harvey-White","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program (PGQP), Beltsville, MD 20708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2297-6490","authenticated-orcid":false,"given":"Shreena","family":"Pradhan","sequence":"additional","affiliation":[{"name":"Institute of Plant Breeding, Genetics and Genomics, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrien","family":"Devos","sequence":"additional","affiliation":[{"name":"Institute of Plant Breeding, Genetics and Genomics, Athens, GA 30602, USA"},{"name":"Department of Crop and Soil Sciences, University of Georgia, Athens, GA 30602, USA"},{"name":"Department of Plant Biology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Devorshak","sequence":"additional","affiliation":[{"name":"USDA-APHIS-PPQ, Field Operations, Raleigh, NC 27606, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"Foster","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program (PGQP), Beltsville, MD 20708, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4840-9609","authenticated-orcid":false,"given":"Bishwo N.","family":"Adhikari","sequence":"additional","affiliation":[{"name":"United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), Plant Protection and Quarantine (PPQ), Plant Germplasm Quarantine Program 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(2020). Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Seashore Paspalum (Paspalum vaginatum). BMC Genom., 21.","DOI":"10.1186\/s12864-020-6508-1"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1104\/pp.20.00796","article-title":"The Halophyte Seashore Paspalum Uses Adaxial Leaf Papillae for Sodium Sequestration","volume":"184","author":"Spiekerman","year":"2020","journal-title":"Plant Physiol."},{"key":"ref_4","unstructured":"Gates, M. (2001). Plant Guide Seashore Paspalum Paspalum vaginatum Sw. Plant Symbol = PAVA."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"305","DOI":"10.21273\/JASHS04737-19","article-title":"Morphological and Physiological Responses of Seashore Paspalum and Bermudagrass to Waterlogging Stress","volume":"144","author":"Xiao","year":"2019","journal-title":"J. Am. Soc. Hortic. Sci."},{"key":"ref_6","unstructured":"Duncan, R.R., and Carrow, R.N. (2000). Seashore Paspalum: The Environmental Turfgrass, Wiley."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"175","DOI":"10.2134\/itsrj2016.05.0411","article-title":"Evaluation of Seashore Paspalum Germplasm for Resistance to Dollar Spot","volume":"13","author":"Steketee","year":"2017","journal-title":"Int. Turfgrass Soc. Res. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.virusres.2008.07.028","article-title":"Plant Virus Emergence and Evolution: Origins, New Encounter Scenarios, Factors Driving Emergence, Effects of Changing World Conditions, and Prospects for Control","volume":"141","author":"Jones","year":"2009","journal-title":"Virus Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.virusres.2012.07.018","article-title":"Australian Monocot-Infecting Mastrevirus Diversity Rivals That in Africa","volume":"169","author":"Kraberger","year":"2012","journal-title":"Virus Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/s00705-011-1129-2","article-title":"Paspalum Striate Mosaic Virus: An Australian Mastrevirus from Paspalum dilatatum","volume":"157","author":"Geering","year":"2012","journal-title":"Arch. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Harmon, P. (2014). Mosaic Disease of St. Augustinegrass Caused by Sugarcane Mosaic Virus, University of Florida IFAS Extension.","DOI":"10.32473\/edis-pp313-2014"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1007\/s00705-007-0016-3","article-title":"Novel Sugarcane Streak and Sugarcane Streak Reunion Mastreviruses from Southern Africa and La R\u00e9union","volume":"153","author":"Shepherd","year":"2008","journal-title":"Arch. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Sastry, K.S., Mandal, B., Hammond, J., Scott, S.W., and Briddon, R.W. (2019). Paspalum dilatatum (Paspalum). Encyclopedia of Plant Viruses and Viroids, Springer.","DOI":"10.1007\/978-81-322-3912-3"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Diaz-Lara, A., Stevens, K., Aguilar-Molina, V.H., Fern\u00e1ndez-Cort\u00e9s, J.M., Chabacano Le\u00f3n, V.M., De Donato, M., Sharma, A., Erickson, T.M., and Al Rwahnih, M. (2023). High-Throughput Sequencing of Grapevine in Mexico Reveals a High Incidence of Viruses Including a New Member of the Genus Enamovirus. Viruses, 15.","DOI":"10.3390\/v15071561"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1094\/PHYTO-07-18-0257-RVW","article-title":"High Throughput Sequencing for Plant Virus Detection and Discovery","volume":"109","author":"Villamor","year":"2019","journal-title":"Phytopathology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1007\/s00705-022-05525-4","article-title":"Two New Umbravirus-like Associated RNAs (UlaRNAs) Discovered in Maize and Johnsongrass from Ecuador","volume":"167","author":"Avila","year":"2022","journal-title":"Arch. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Liu, J., Carino, E., Bera, S., Gao, F., May, J.P., and Simon, A.E. (2021). Structural Analysis and Whole Genome Mapping of a New Type of Plant Virus Subviral RNA: Umbravirus-like Associated RNAs. Viruses, 13.","DOI":"10.3390\/v13040646"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Malapi-Wight, M., Adhikari, B., Zhou, J., Hendrickson, L., Maroon-Lango, C.J., McFarland, C., Foster, J.A., and Hurtado-Gonzales, O.P. (2021). Hts-Based Diagnostics of Sugarcane Viruses: Seasonal Variation and Its Implications for Accurate Detection. Viruses, 13.","DOI":"10.3390\/v13081627"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s40168-023-01500-6","article-title":"In-Depth Study of Tomato and Weed Viromes Reveals Undiscovered Plant Virus Diversity in an Agroecosystem","volume":"11","author":"Rivarez","year":"2023","journal-title":"Microbiome"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1007\/s00705-023-05763-0","article-title":"Complete Genome Sequence of Zoysia Mosaic Virus, a Novel Member of the Genus Poacevirus","volume":"168","author":"Adhikari","year":"2023","journal-title":"Arch. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/mpp.13163","article-title":"Potato Virus X: A Global Potato-Infecting Virus and Type Member of the Potexvirus Genus","volume":"23","author":"Verchot","year":"2022","journal-title":"Mol. Plant Pathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1007\/s13337-024-00898-3","article-title":"Identification of Nine Putative Novel Members of Plant-Infecting Alphaflexiviruses in Public Domain Plant Transcriptomes","volume":"35","author":"Sravani","year":"2024","journal-title":"Virusdisease"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1006\/viro.1997.8565","article-title":"Mutations That Alter a Conserved Element Upstream of the Potato Virus X Triple Block and Coat Protein Genes Affect Subgenomic RNA Accumulation","volume":"232","author":"Kim","year":"1997","journal-title":"Virology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e11795","DOI":"10.7554\/eLife.11795","article-title":"The Near-Atomic CryoEM Structure of a Flexible Filamentous Plant Virus Shows 1 Homology of Its Coat Protein with Nucleoproteins of Animal Viruses 2","volume":"4","author":"Agirrezabala","year":"2015","journal-title":"Elife"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1007\/s00705-008-0108-8","article-title":"Molecular Characterization of Lolium Latent Virus, Proposed Type Member of a New Genus in the Family Flexiviridae","volume":"153","author":"Vaira","year":"2008","journal-title":"Arch. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1099\/vir.0.042960-0","article-title":"Lolium Latent Virus (Alphaflexiviridae) Coat Proteins: Expression and Functions in Infected Plant Tissue","volume":"93","author":"Vaira","year":"2012","journal-title":"J. Gen. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1146\/annurev.phyto.45.062806.094401","article-title":"Family Flexiviridae: A Case Study in Virion and Genome Plasticity","volume":"45","author":"Martelli","year":"2008","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Chen, I.H., Huang, Y.W., and Tsai, C.H. (2017). The Functional Roles of the Cis-Acting Elements in Bamboo Mosaic Virus RNA Genome. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00645"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1099\/vir.0.82667-0","article-title":"Molecular Biology of Potexviruses: Recent Advances","volume":"88","author":"Ye","year":"2007","journal-title":"J. Gen. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.jmb.2012.05.032","article-title":"Crystal Structure of the Coat Protein of the Flexible Filamentous Papaya Mosaic Virus","volume":"422","author":"Yang","year":"2012","journal-title":"J. Mol. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s13337-017-0362-z","article-title":"Population Genetic Analysis of Potato Virus X Based on the CP Gene Sequence","volume":"28","author":"Hajizadeh","year":"2017","journal-title":"Virusdisease"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Fuentes, S., Gibbs, A.J., Hajizadeh, M., Perez, A., Adams, I.P., Fribourg, C.E., Kreuze, J., Fox, A., Boonham, N., and Jones, R.A.C. (2021). The Phylogeography of Potato Virus X Shows the Fingerprints of Its Human Vector. Viruses, 13.","DOI":"10.3390\/v13040644"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1134\/S0026893306040157","article-title":"Regulation of RNA Translation in Potato Virus X RNA-Coat Protein Complexes: The Key Role of the N-Terminal Segment of the Protein","volume":"40","author":"Karpova","year":"2006","journal-title":"Mol. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1046\/j.1365-313X.1992.t01-24-00999.x","article-title":"Potato Virus X as a Vector for Gene Expression in Plants","volume":"2","author":"Chapman","year":"1992","journal-title":"Plant J."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1105\/tpc.10.4.495","article-title":"Cell-to-Cell and Phloem-Mediated Transport of Potato Virus X: The Role of Virions","volume":"10","author":"Cruz","year":"1998","journal-title":"Plant Cell"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JVI.01268-18","article-title":"Analysis of Fitness Trade-Offs in the Host Range Expansion of an RNA Virus, Tobacco Mild Green Mosaic Virus","volume":"92","author":"Bera","year":"2018","journal-title":"J. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/aab.12456","article-title":"Description and Genetic Variation of a Distinct Species of Potyvirus Infecting Saffron (Crocus sativus L.) Plants in Major Production Regions in Iran","volume":"173","author":"Parizad","year":"2018","journal-title":"Ann. Appl. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.2135\/cropsci2017.04.0241","article-title":"Ploidy Level and Genetic Diversity in the Genus Paspalum, Group Disticha","volume":"57","author":"Eudy","year":"2017","journal-title":"Crop Sci."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Hu, X., Hurtado-Gonzales, O.P., Adhikari, B.N., French-Monar, R.D., Malapi, M., Foster, J.A., and McFarland, C.D. (2023). PhytoPipe: A Phytosanitary Pipeline for Plant Pathogen Detection and Diagnosis Using RNA-Seq Data. BMC Bioinform., 24.","DOI":"10.1186\/s12859-023-05589-2"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-Length Transcriptome Assembly from RNA-Seq Data without a Reference Genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., and Madden, T.L. (2009). BLAST+: Architecture and Applications. BMC Bioinform., 10.","DOI":"10.1186\/1471-2105-10-421"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"530","DOI":"10.12688\/f1000research.18776.1","article-title":"RVDB-Prot, a Reference Viral Protein Database and Its HMM Profiles","volume":"8","author":"Bigot","year":"2019","journal-title":"F1000Research"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and Sensitive Protein Alignment Using DIAMOND","volume":"12","author":"Buchfink","year":"2014","journal-title":"Nat. Methods"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Muhire, B.M., Varsani, A., and Martin, D.P. (2014). SDT: A Virus Classification Tool Based on Pairwise Sequence Alignment and Identity Calculation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0108277"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"D384","DOI":"10.1093\/nar\/gkac1096","article-title":"The Conserved Domain Database in 2023","volume":"51","author":"Wang","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1038\/s41592-022-01488-1","article-title":"ColabFold: Making Protein Folding Accessible to All","volume":"19","author":"Mirdita","year":"2022","journal-title":"Nat. Methods"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1093\/nar\/gki524","article-title":"TM-Align: A Protein Structure Alignment Algorithm Based on the TM-Score","volume":"33","author":"Zhang","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/pro.3943","article-title":"UCSF ChimeraX: Structure Visualization for Researchers, Educators, and Developers","volume":"30","author":"Pettersen","year":"2021","journal-title":"Protein Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"veaa087","DOI":"10.1093\/ve\/veaa087","article-title":"RDP5: A Computer Program for Analyzing Recombination in, and Removing Signals of Recombination from, Nucleotide Sequence Datasets","volume":"7","author":"Martin","year":"2021","journal-title":"Virus Evol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1093\/bioinformatics\/btp187","article-title":"DnaSP v5: A Software for Comprehensive Analysis of DNA Polymorphism Data","volume":"25","author":"Librado","year":"2009","journal-title":"Bioinformatics"},{"key":"ref_53","first-page":"1358","article-title":"Estimating F-Statistics for the Analysis of Population Structure","volume":"38","author":"Weir","year":"1984","journal-title":"Evolution"},{"key":"ref_54","unstructured":"Shadow, R.A. (2004). Seashore Paspalum Panicum vaginatum Sw. Plant Symbol = PAVA, Plant Fact Sheet."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.coviro.2016.01.008","article-title":"Environment and Evolution Modulate Plant Virus Pathogenesis","volume":"17","author":"Fraile","year":"2016","journal-title":"Curr. Opin. Virol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1111\/nph.13631","article-title":"Environment and Host Genotype Determine the Outcome of a Plant-Virus Interaction: From Antagonism to Mutualism","volume":"209","author":"Hily","year":"2016","journal-title":"New Phytol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/s00705-023-05934-z","article-title":"Long-Term Passages of Plantago Asiatica Mosaic Virus Alter Virulence and Multiplication in Nicotiana benthamiana Plants","volume":"169","author":"Nakamura","year":"2024","journal-title":"Arch. Virol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3345","DOI":"10.1094\/PDIS-03-24-0617-RE","article-title":"An Update in Claviceps paspali Disease: A Comprehensive Analysis on Field and Greenhouse Paspalum spp. Infection","volume":"108","author":"Oberti","year":"2024","journal-title":"Plant Dis."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5760\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T04:13:17Z","timestamp":1782619997000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":58,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ijms27135760"],"URL":"https:\/\/doi.org\/10.3390\/ijms27135760","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]}}}