{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T01:44:31Z","timestamp":1782783871043,"version":"3.54.5"},"reference-count":44,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ANID-FONDECYT Regular 2024","award":["1240030"],"award-info":[{"award-number":["1240030"]}]},{"name":"Department of Research and Development of ADL Diagnostic Chile"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Renibacterium salmoninarum, the etiological agent of bacterial kidney disease, is a facultative intracellular pathogen whose interaction with salmonid phagocytic cells remains poorly resolved at the protein level. Here, we aimed to define the temporal protein-abundance architecture of SHK-1 macrophage-like cells after R. salmoninarum inoculation and to test whether this response supports broad canonical cell-death pathway engagement. We used label-free quantitative LC-MS\/MS proteomics to profile SHK-1 cells over a 48 h post-inoculation time course. Because the design included a single non-infected T0 baseline, analyses were framed as baseline-referenced post-inoculation comparisons rather than a fully controlled mock time course. Of 6842 proteins retained for statistical modeling, 2254 were strictly differentially abundant in at least one contrast relative to T0 (adjusted p &lt; 0.05 and |log2FC| \u2265 0.585). Perturbation was strongest at 1\u20132 h and progressively contracted at later time points. Among 1278 recurrent proteins, k-means clustering resolved four temporal modules capturing coordinated remodeling of lysosomal, immunometabolic, cytoskeletal, stress-response, and antioxidant programs. A curated cell-death panel spanning apoptosis, pyroptosis, necroptosis, ferroptosis, and PANoptosis yielded only three detected markers; CASP3 and MLKL met the strict threshold, whereas ACSL4 remained sub-threshold. Overall, early host-cell remodeling, rather than broad canonical death-program execution, was the predominant proteomic signature of SHK-1 cells during the first 48 h after R. salmoninarum inoculation.<\/jats:p>","DOI":"10.3390\/ijms27135773","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:55:11Z","timestamp":1782780911000},"page":"5773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Time-Resolved Label-Free Proteomics of SHK-1 Cells After Renibacterium salmoninarum Inoculation Reveals Early Host-Cell Remodeling"],"prefix":"10.3390","volume":"27","author":[{"given":"Jorge F.","family":"Beltr\u00e1n","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Faculty of Engineering and Science, Universidad de La Frontera, Ave. Francisco Salazar 01145, Temuco 4811230, Chile"},{"name":"BioChemIntelli, Bioinformatics Research & Development, Temuco 4780000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rn","family":"Bethke","sequence":"additional","affiliation":[{"name":"Aquit SpA, Av. Libertad 269 of 904, Vi\u00f1a del Mar 2520000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Flores-Martin","sequence":"additional","affiliation":[{"name":"Keybio Solution E.I.R.L., Valdivia 5090000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia A.","family":"Barrientos","sequence":"additional","affiliation":[{"name":"Keybio Solution E.I.R.L., Valdivia 5090000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Keybio Solution E.I.R.L., Valdivia 5090000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2168-4029","authenticated-orcid":false,"given":"Adolfo","family":"Isla","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias B\u00e1sicas, Facultad de Ciencias, Universidad Santo Tomas, Valdivia 5090000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Almendras","sequence":"additional","affiliation":[{"name":"Departamento de Investigaci\u00f3n y Desarrollo, Greenvolution SpA, Puerto Varas 5550000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0100-2190","authenticated-orcid":false,"given":"Marcos","family":"Mancilla","sequence":"additional","affiliation":[{"name":"ADL Diagnostic Chile, Sector la Vara, Puerto Montt 5480000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4619-4600","authenticated-orcid":false,"given":"Alejandro J.","family":"Ya\u00f1ez","sequence":"additional","affiliation":[{"name":"Keybio Solution E.I.R.L., Valdivia 5090000, Chile"},{"name":"Departamento de Investigaci\u00f3n y Desarrollo, Greenvolution SpA, Puerto Varas 5550000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"735089","DOI":"10.1016\/j.aquaculture.2020.735089","article-title":"Production cost and competitiveness in major salmon farming countries 2003\u20132018","volume":"522","author":"Iversen","year":"2020","journal-title":"Aquaculture"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1146\/annurev-marine-010814-015646","article-title":"Infectious Diseases Affect Marine Fisheries and Aquaculture Economics","volume":"7","author":"Lafferty","year":"2015","journal-title":"Annu. 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