{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T01:44:24Z","timestamp":1782783864183,"version":"3.54.5"},"reference-count":30,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"2025 Qinghai Provincial Basic Research Project","award":["2025-ZJ-911Q"],"award-info":[{"award-number":["2025-ZJ-911Q"]}]},{"name":"2025 Qinghai Provincial Basic Research Project","award":["2025-ZJ-748"],"award-info":[{"award-number":["2025-ZJ-748"]}]},{"DOI":"10.13039\/501100010230","name":"Qinghai University Research Ability Enhancement Project","doi-asserted-by":"publisher","award":["2026KTSQ15"],"award-info":[{"award-number":["2026KTSQ15"]}],"id":[{"id":"10.13039\/501100010230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Camel milk is increasingly recognized as a premium functional food, attributed to its rich nutraceutical compounds. Recent research has concentrated on the nanoscale extracellular vesicles derived from camel milk (CM-EVs), which exhibit distinctive properties. This review examines the methodologies for isolating and characterizing CM-EVs, alongside their potential health benefits in functional foods and nutraceuticals. CM-EVs have the capacity to safeguard functional proteins, noncoding RNAs, and bioactive lipids from degradation within the gastrointestinal tract, rendering them particularly suitable for incorporation into infant formulas, adult dietary supplements, and nutraceuticals targeting chronic inflammatory and metabolic disorders. Preclinical models indicate that CM-EVs can mitigate oxidative stress, enhance intestinal barrier integrity, and modulate gut microbiota, thereby contributing to the reduction in colonic injury and inflammation. Nonetheless, the majority of these findings are derived from laboratory and animal studies, highlighting a substantial deficiency in human clinical trials. Critical research gaps remain, necessitating further investigation into the elucidation of molecular mechanisms, assessment of long-term safety, evaluation of bioavailability, and compatibility with dairy processing techniques. This review underscores the significance of CM-EVs as bioactive food components and delineates research priorities, such as standardizing isolation methods, investigating food matrix integration, and providing translational evidence for their application in nutrition and preventive medicine.<\/jats:p>","DOI":"10.3390\/ijms27135777","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:55:11Z","timestamp":1782780911000},"page":"5777","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Camel Milk Extracellular Vesicles as Functional Foods and Nutraceuticals: Bridging Dairy Science and Chronic Disease Prevention"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2226-3392","authenticated-orcid":false,"given":"Hui","family":"Yang","sequence":"first","affiliation":[{"name":"College of Medical, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5791-4629","authenticated-orcid":false,"given":"Yajun","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Medical, Qinghai University, Xining 810016, China"},{"name":"School of Public Health, Peking University, Beijing 100191, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rili","family":"Ge","sequence":"additional","affiliation":[{"name":"College of Medical, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102729","DOI":"10.1016\/j.fochx.2025.102729","article-title":"A new insight into the exosome protein and lipid composition in camel colostrum and mature milk using comparative proteome and lipidomics analyses","volume":"29","author":"Ming","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1021\/acs.jafc.5c11226","article-title":"Therapeutic effects of camel milk exosomes and their miR-148a-3p cargo on DSS-induced colitis: Modulating gut inflammation through the SIRT1\/NF-\u03baB pathway and microbiota alterations","volume":"74","author":"Yu","year":"2026","journal-title":"J. Agric. Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102592","DOI":"10.1016\/j.fochx.2025.102592","article-title":"Comprehensive analysis through multi-omics integration to compare and elucidate the specific substances in milk of donkey, horse, camel, human and pig","volume":"28","author":"Zhang","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Yang, H., Er, D., Wang, Y.H., Zhai, B.T., and Ge, R. (2025). Camel milk-derived extracellular vesicles as a functional food component ameliorate hypobaric hypoxia-induced colonic injury through microbiota-metabolite crosstalk. Nutrients, 17.","DOI":"10.3390\/nu17152431"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Yang, B., Du, S., Liu, L., Wang, J., and Er, D. (2025). Camel milk exosomes regulate glucose metabolism by inhibiting mitochondrial complex I in hepatocytes. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-04555-9"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"113980","DOI":"10.1016\/j.jconrel.2025.113980","article-title":"Camel milk extracellular vesicles-mediated co-delivery of siVEGF\/sorafenib enhances the anti-hepatocellular carcinoma activity by dual-effect blocking the biological effects of VEGF","volume":"385","author":"Chen","year":"2025","journal-title":"J. Control. Release"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1039\/D4FO04331F","article-title":"Camel milk extracellular vesicles\/exosomes: A fascinating frontier in isolation and therapeutic potential","volume":"16","author":"Badawy","year":"2025","journal-title":"Food Funct."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Shaban, A.M., Raslan, M., Sharawi, Z.W., Abdelhameed, M.S., Hammouda, O., El-Masry, H.M., Elsayed, K.N.M., and El-Magd, M.A. (2023). Antibacterial, antifungal, and anticancer effects of camel milk exosomes: An in vitro study. Vet. Sci., 10.","DOI":"10.3390\/vetsci10020124"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1038\/s41538-025-00375-1","article-title":"Milk-derived extracellular vesicles and gut health","volume":"9","author":"Muttiah","year":"2025","journal-title":"npj Sci. Food"},{"key":"ref_10","first-page":"321","article-title":"Assessment of the anti-cancer effects of camel milk exosomes (CMEXOs) on murine colorectal cancer cell line (CT-26)","volume":"23","author":"Karbasi","year":"2024","journal-title":"Iran. J. Allergy Asthma Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"132","DOI":"10.17305\/bb.2024.11267","article-title":"Camel milk-derived exosomes as novel nanocarriers for curcumin delivery in lung cancer","volume":"26","author":"Saeed","year":"2024","journal-title":"Biomol. Biomed."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8304","DOI":"10.1021\/acs.jafc.3c07899","article-title":"Milk-derived extracellular vesicles: Biomedical applications, current challenges, and future perspectives","volume":"72","author":"Salehi","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e70947","DOI":"10.1096\/fj.202501630R","article-title":"Milk-derived extracellular vesicles and microRNAs: Potential modulators of intestinal homeostasis","volume":"39","author":"Husseini","year":"2025","journal-title":"FASEB J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1007\/s11095-022-03404-w","article-title":"Review: Milk small extracellular vesicles for use in the delivery of therapeutics","volume":"40","author":"Munir","year":"2023","journal-title":"Pharm. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"iyae088","DOI":"10.1093\/genetics\/iyae088","article-title":"Extracellular vesicles","volume":"227","author":"Wang","year":"2024","journal-title":"Genetics"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Weiskirchen, R., Schr\u00f6der, S.K., Weiskirchen, S., Buhl, E.M., and Melnik, B. (2023). Isolation of bovine and human milk extracellular vesicles. Biomedicines, 11.","DOI":"10.3390\/biomedicines11102715"},{"key":"ref_17","first-page":"80","article-title":"Isolation and analysis methods of extracellular vesicles (EVs)","volume":"2","author":"Zhao","year":"2021","journal-title":"Extracell. Vesicles Circ. Nucleic Acids"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"342759","DOI":"10.1016\/j.aca.2024.342759","article-title":"Oxylipin profile of human milk and human milk-derived extracellular vesicles","volume":"1313","author":"Kuligowski","year":"2024","journal-title":"Anal. Chim. Acta"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7523","DOI":"10.1039\/C9FO01914F","article-title":"Camel milk exosomes modulate cyclophosphamide-induced oxidative stress and immuno-toxicity in rats","volume":"10","author":"Ibrahim","year":"2019","journal-title":"Food Funct."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1556\/004.2023.00869","article-title":"Sequencing and analysis of micro RNAs in camel milk exosomes","volume":"71","author":"Wu","year":"2023","journal-title":"Acta Vet. Hung."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"114615","DOI":"10.1016\/j.biopha.2023.114615","article-title":"The potential application of encapsulated exosomes: A new approach to increase exosomes therapeutic efficacy","volume":"162","author":"Hazrati","year":"2023","journal-title":"Biomed. Pharmacother."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1002\/fsn3.3749","article-title":"Homogenization and thermal processing reduce the concentration of extracellular vesicles in bovine milk","volume":"12","author":"Colella","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1093\/jn\/nxab031","article-title":"Regular industrial processing of bovine milk impacts the integrity and molecular composition of extracellular vesicles","volume":"151","author":"Kleinjan","year":"2021","journal-title":"J. Nutr."},{"key":"ref_24","unstructured":"Fan, S. (2020). Isolation and Identification of Milk-Derived Exosomes from Different Animals and Their Proteomic Analysis. [Master\u2019s Thesis, Chinese Academy of Agricultural Sciences]."},{"key":"ref_25","first-page":"796","article-title":"Camel milk for food allergies in children","volume":"7","author":"Shabo","year":"2005","journal-title":"Isr. Med. Assoc. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"255","DOI":"10.2500\/aap.2011.32.3429","article-title":"Camel milk: An alternative for cow\u2019s milk allergy in children","volume":"32","author":"Ehlayel","year":"2011","journal-title":"Allergy Asthma Proc."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Wang, J., Ren, W., Liu, S., Li, Z., Zeng, Y., Meng, J., and Yao, X. (2025). Changes in antioxidant capacity and gut microbiota in mice after intake of camel milk. Front. Cell. Infect. Microbiol., 15.","DOI":"10.3389\/fcimb.2025.1621031"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e70098","DOI":"10.1002\/jev2.70098","article-title":"Metabolically engineered extracellular vesicles released from a composite hydrogel delivery system regulate the microenvironment for periprosthetic osteolysis treatment","volume":"14","author":"Wang","year":"2025","journal-title":"J. Extracell. Vesicles"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"112220","DOI":"10.1016\/j.biopha.2021.112220","article-title":"Therapeutic potential of camel milk exosomes against HepaRG cells with potent apoptotic, anti-inflammatory, and anti-angiogenesis effects for colostrum exosomes","volume":"143","author":"Algezawy","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"C379","DOI":"10.1152\/ajpcell.00816.2025","article-title":"Plasma extracellular vesicle signatures of metabolic health and exercise response in a pilot study of older adults","volume":"330","author":"Zhang","year":"2026","journal-title":"Am. J. Physiol. Cell Physiol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5777\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:59:03Z","timestamp":1782781143000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5777"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":30,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ijms27135777"],"URL":"https:\/\/doi.org\/10.3390\/ijms27135777","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]}}}