{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:54:10Z","timestamp":1789070050797,"version":"build-2803163510"},"reference-count":56,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Interdisciplinary Research Project of Hangzhou Normal University","award":["2025JCXK01"],"award-info":[{"award-number":["2025JCXK01"]}]},{"award":["2025JCXK01"],"award-info":[{"award-number":["2025JCXK01"]}],"id":[{"id":"https:\/\/ror.org\/014v1mr15","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Tianchi Talents Program of Xinjiang Province","award":["YHBH01003732"],"award-info":[{"award-number":["YHBH01003732"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Small heat shock proteins (Hsp20s) function as essential molecular chaperones in plant stress responses, yet their genome-wide characterization in sunflower (Helianthus annuus L.) remains lacking and their functional role in heat response is also unknown. In this study, 65 HaHsp20 genes were identified in sunflower through a comprehensive genome-wide analysis based on the conserved ACD (\u03b1-crystallin) domain. The expansion of this family was primarily driven by whole-genome duplication (WGD) or segmental duplication events, with the CI subfamily (20 members) representing the most significantly expanded lineage-specific clade. While all HaHsp20 proteins harbor the conserved \u03b1-crystallin domain (ACD), they exhibit diverse molecular weights (11.31\u201353.35 kDa), isoelectric points (4.71\u20139.75), and subcellular localization patterns. Promoter cis-regulatory element analysis revealed a predominance of ABA and MeJA-responsive elements but only two canonical heat shock elements. Transcriptome and RT-qPCR analyses revealed that most HaHsp20 genes are responsive to heat stress, with seven HaHsp20 genes exhibiting extremely upregulated expression (more than 1000-fold) after 10 h of 45 \u00b0C treatment. Among these, HaHsp21.59 and HaHsp25.91 showed an increase of over 4000-fold in expression. These findings provide a comprehensive foundation for understanding the evolutionary history and expression dynamics of the HaHsp20 family in sunflower, and highlight HaHsp21.59 and HaHsp25.91 as promising candidate genes for future functional validation of their potential roles in heat stress tolerance.<\/jats:p>","DOI":"10.3390\/ijms27135799","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:55:11Z","timestamp":1782780911000},"page":"5799","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genome-Wide Identification of the Hsp20 Family Responding to Heat Stress in Sunflower (Helianthus annuus L.)"],"prefix":"10.3390","volume":"27","author":[{"given":"Yushan","family":"Liu","sequence":"first","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2897-3851","authenticated-orcid":false,"given":"Shurui","family":"Dong","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenning","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolei","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyang","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maohong","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4368-808X","authenticated-orcid":false,"given":"Tao","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Science, Hangzhou Normal University, Hangzhou 311121, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"El-Sappah, A.H., Rather, S.A., Wani, S.H., Elrys, A.S., Bilal, M., Huang, Q., Dar, Z.A., Elashtokhy, M.M.A., Soaud, N., and Koul, M. (2022). Heat Stress-Mediated Constraints in Maize (Zea mays) Production: Challenges and Solutions. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.879366"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Wen, B. (2015). Effects of High Temperature and Water Stress on Seed Germination of the Invasive Species Mexican Sunflower. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0141567"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"131621","DOI":"10.1016\/j.jhazmat.2023.131621","article-title":"Transgenic soybean of GsMYB10 shapes rhizosphere microbes to promote resistance to aluminum (Al) toxicity","volume":"455","author":"Liu","year":"2023","journal-title":"J. Hazard. Mater."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1146\/annurev-arplant-050718-095919","article-title":"Thermomorphogenesis","volume":"70","author":"Casal","year":"2019","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"106283","DOI":"10.1016\/j.envexpbot.2025.106283","article-title":"Unveiling the impact of heat and drought stress on plant reproductive tissues","volume":"241","author":"Zhou","year":"2026","journal-title":"Environ. Exp. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1093\/pcp\/pcp135","article-title":"High Temperatures Cause Male Sterility in Rice Plants with Transcriptional Alterations During Pollen Development","volume":"50","author":"Endo","year":"2009","journal-title":"Plant Cell Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1626\/pps.3.430","article-title":"High Temperature at Flowering Inhibits Swelling of Pollen Grains, a Driving Force for Thecae Dehiscence in Rice (Oryza sativa L.)","volume":"3","author":"Matsui","year":"2000","journal-title":"Plant Prod. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1093\/pcp\/pcq034","article-title":"Atlas of Rice Grain Filling-Related Metabolism under High Temperature: Joint Analysis of Metabolome and Transcriptome Demonstrated Inhibition of Starch Accumulation and Induction of Amino Acid Accumulation","volume":"51","author":"Yamakawa","year":"2010","journal-title":"Plant Cell Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.rsci.2023.06.003","article-title":"Translocation and Distribution of Carbon-Nitrogen in Relation to Rice Yield and Grain Quality as Affected by High Temperature at Early Panicle Initiation Stage","volume":"30","author":"Ji","year":"2023","journal-title":"Rice Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1104\/pp.103.2.309","article-title":"Dynamic regulation of chloroplast transcription","volume":"103","author":"Mullet","year":"1993","journal-title":"Plant Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1111\/nph.70661","article-title":"High-temperature acclimation of photosystem II in land plants","volume":"249","author":"Posch","year":"2026","journal-title":"New Phytol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/s41477-024-01859-w","article-title":"Stomatal opening under high temperatures is controlled by the OST1-regulated TOT3\u2013AHA1 module","volume":"11","author":"Xu","year":"2025","journal-title":"Nat. Plants"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Posp\u00ed\u0161il, P. (2016). Production of Reactive Oxygen Species by Photosystem II as a Response to Light and Temperature Stress. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.01950"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.envexpbot.2017.05.002","article-title":"Physiological and biochemical responses to high light and temperature stress in plants","volume":"139","author":"Orzechowska","year":"2017","journal-title":"Environ. Exp. Bot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1111\/jipb.13056","article-title":"The calcium-dependent protein kinase ZmCDPK7 functions in heat-stress tolerance in maize","volume":"63","author":"Zhao","year":"2021","journal-title":"J. Integr. Plant Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1111\/pce.14609","article-title":"Nanoscale simulation of the thylakoid membrane response to extreme temperatures","volume":"46","author":"Kulke","year":"2023","journal-title":"Plant Cell Environ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1111\/pbi.13297","article-title":"A membrane-associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice","volume":"18","author":"Liu","year":"2020","journal-title":"Plant Biotechnol. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1016\/j.hpj.2022.12.006","article-title":"Heat-inducible SlWRKY3 confers thermotolerance by activating the SlGRXS1 gene cluster in tomato","volume":"10","author":"Wang","year":"2024","journal-title":"Hortic. Plant J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"uhad089","DOI":"10.1093\/hr\/uhad089","article-title":"ABI5 promotes heat stress-induced chlorophyll degradation by modulating the stability of MYB44 in cucumber","volume":"10","author":"Liu","year":"2023","journal-title":"Hortic. Res."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Guo, M., Liu, J.H., Ma, X., Luo, D.X., Gong, Z.H., and Lu, M.H. (2016). The Plant Heat Stress Transcription Factors (HSFs): Structure, Regulation, and Function in Response to Abiotic Stresses. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00114"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e161","DOI":"10.1002\/mco2.161","article-title":"Heat shock proteins: Biological functions, pathological roles, and therapeutic opportunities","volume":"3","author":"Hu","year":"2022","journal-title":"MedComm"},{"key":"ref_22","first-page":"22","article-title":"Integration of genome-wide identification, transcriptome and association analysis of HSP20 gene family to revealing genetic basis of floral organ number-related traits in tree peony","volume":"3","author":"Peng","year":"2023","journal-title":"Ornam. Plant Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1146\/annurev.pp.42.060191.003051","article-title":"The Roles of Heat Shock Proteins in Plants","volume":"42","author":"Vierling","year":"1991","journal-title":"Annu. Rev. Plant Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1093\/jxb\/ers355","article-title":"The evolution, function, structure, and expression of the plant sHSPs","volume":"64","author":"Waters","year":"2013","journal-title":"J. Exp. Bot."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1111\/nph.16536","article-title":"Plant small heat shock proteins\u2014Evolutionary and functional diversity","volume":"227","author":"Waters","year":"2020","journal-title":"New Phytol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1379\/1466-1268(2001)006<0225:TEFOAT>2.0.CO;2","article-title":"The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing \u03b1-crystallin domains (Acd proteins)","volume":"6","author":"Scharf","year":"2001","journal-title":"Cell Stress Chaperones"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"129001","DOI":"10.1016\/j.ijbiomac.2023.129001","article-title":"Comprehensive analysis of Dendrobium catenatum HSP20 family genes and functional characterization of DcHSP20-12 in response to temperature stress","volume":"258","author":"Wang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.cj.2025.11.021","article-title":"Natural variation in ZmHSP20-5 enhances root growth and drought tolerance in maize","volume":"14","author":"Zhang","year":"2026","journal-title":"Crop J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.plaphy.2019.07.001","article-title":"A small heat shock protein CaHsp25.9 positively regulates heat, salt, and drought stress tolerance in pepper (Capsicum annuum L.)","volume":"142","author":"Feng","year":"2019","journal-title":"Plant Physiol. Biochem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e14288","DOI":"10.1111\/ppl.14288","article-title":"Identification of Hsp20 gene family in Malus domestica and functional characterization of Hsp20 class I gene MdHsp18.2b","volume":"176","author":"Zhang","year":"2024","journal-title":"Physiol. Plant."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Sarkar, N.K., Kim, Y.-K., and Grover, A. (2009). Rice sHsp genes: Genomic organization and expression profiling under stress and development. BMC Genom., 10.","DOI":"10.1186\/1471-2164-10-393"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e6832","DOI":"10.7717\/peerj.6832","article-title":"Genome-wide identification and expression profile analysis of the Hsp20 gene family in Barley (Hordeum vulgare L.)","volume":"7","author":"Li","year":"2019","journal-title":"PeerJ"},{"key":"ref_33","unstructured":"Intergovernmental Panel on Climate Change (2014). Climate Change 2014\u2014Impacts, Adaptation and Vulnerability: Part A: Global and Sectoral Aspects: Working Group II Contribution to the IPCC Fifth Assessment Report: Volume 1: Global and Sectoral Aspects, Cambridge University Press."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/nature22380","article-title":"The sunflower genome provides insights into oil metabolism, flowering and Asterid evolution","volume":"546","author":"Badouin","year":"2017","journal-title":"Nature"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1007\/s00709-023-01862-6","article-title":"HSF and Hsp Gene Families in sunflower: A comprehensive genome-wide determination survey and expression patterns under abiotic stress conditions","volume":"260","author":"Ceylan","year":"2023","journal-title":"Protoplasma"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"111355","DOI":"10.1016\/j.plaphy.2026.111355","article-title":"The sunflower HaHB4 gene confers enhanced tolerance to heat stress at the pre-anthesis stage in three different wheat cultivars","volume":"234","author":"Curin","year":"2026","journal-title":"Plant Physiol. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1007\/s11103-009-9477-y","article-title":"Comprehensive sequence and expression profile analysis of Hsp20 gene family in rice","volume":"70","author":"Ouyang","year":"2009","journal-title":"Plant Mol. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Lopes-Caitar, V.S., de Carvalho, M.C.C.G., Darben, L.M., Kuwahara, M.K., Nepomuceno, A.L., Dias, W.P., Abdelnoor, R.V., and Marcelino-Guimar\u00e3es, F.C. (2013). Genome-wide analysis of the Hsp 20 gene family in soybean: Comprehensive sequence, genomic organization and expression profile analysis under abiotic and biotic stresses. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-577"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Cannon, S.B., Mitra, A., Baumgarten, A., Young, N.D., and May, G. (2004). The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana. BMC Plant Biol., 4.","DOI":"10.1186\/1471-2229-4-10"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.gde.2015.11.003","article-title":"Polyploidy and genome evolution in plants","volume":"35","author":"Soltis","year":"2015","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2581","DOI":"10.1038\/s41598-017-01857-3","article-title":"Massive expansion and differential evolution of small heat shock proteins with wheat (Triticum aestivum L.) polyploidization","volume":"7","author":"Wang","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Wu, Q., Wang, Y., and Mu, Z. (2025). Identification of the HSP20 Gene Family in L. barbarum and Their Contrasting Response to Heat Stress Between Two Varieties. Genes, 16.","DOI":"10.3390\/genes16040440"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.tplants.2016.08.015","article-title":"Transcriptional Regulatory Network of Plant Heat Stress Response","volume":"22","author":"Ohama","year":"2017","journal-title":"Trends Plant Sci."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Su, Y., Huang, Y., Dong, X., Wang, R., Tang, M., Cai, J., Chen, J., Zhang, X., and Nie, G. (2021). Exogenous Methyl Jasmonate Improves Heat Tolerance of Perennial Ryegrass Through Alteration of Osmotic Adjustment, Antioxidant Defense, and Expression of Jasmonic Acid-Responsive Genes. Front. Plant Sci., 12.","DOI":"10.3389\/fpls.2021.664519"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Sun, X., Zhu, J., Li, X., Li, Z., Han, L., and Luo, H. (2020). AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response. BMC Plant Biol., 20.","DOI":"10.1186\/s12870-020-02369-5"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Reddy, P.S., Kavi Kishor, P.B., Seiler, C., Kuhlmann, M., Eschen-Lippold, L., Lee, J., Reddy, M.K., and Sreenivasulu, N. (2014). Unraveling regulation of the small heat shock proteins by the heat shock factor HvHsfB2c in barley: Its implications in drought stress response and seed development. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0089125"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.biochi.2026.04.003","article-title":"Small heat shock proteins in plants: Structure, function and role in stress adaptation","volume":"246","author":"Mondal","year":"2026","journal-title":"Biochimie"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1105\/tpc.111.083345","article-title":"High-Resolution Temporal Profiling of Transcripts during Arabidopsis Leaf Senescence Reveals a Distinct Chronology of Processes and Regulation","volume":"23","author":"Breeze","year":"2011","journal-title":"Plant Cell"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1105\/tpc.111.090894","article-title":"JUNGBRUNNEN1, a Reactive Oxygen Species\u2013Responsive NAC Transcription Factor, Regulates Longevity in Arabidopsis","volume":"24","author":"Wu","year":"2012","journal-title":"Plant Cell"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Kaur, H., Petla, B.P., Kamble, N.U., Singh, A., Rao, V., Salvi, P., Ghosh, S., and Majee, M. (2015). Differentially expressed seed aging responsive heat shock protein OsHSP18.2 implicates in seed vigor, longevity and improves germination and seedling establishment under abiotic stress. Front. Plant Sci., 6.","DOI":"10.3389\/fpls.2015.00713"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1007\/s00299-017-2143-y","article-title":"Heterologous expression of three Camellia sinensis small heat shock protein genes confers temperature stress tolerance in yeast and Arabidopsis thaliana","volume":"36","author":"Wang","year":"2017","journal-title":"Plant Cell Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.plaphy.2022.02.017","article-title":"Functional characterization of the Pinellia ternata cytoplasmic class II small heat shock protein gene PtsHSP17.2 via promoter analysis and overexpression in tobacco","volume":"177","author":"Tian","year":"2022","journal-title":"Plant Physiol. Biochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1023\/B:MOLB.0000018764.30795.c1","article-title":"Over-expression of a small heat shock protein, sHSP17.7, confers both heat tolerance and UV-B resistance to rice plants","volume":"13","author":"Murakami","year":"2004","journal-title":"Mol. Breed."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1093\/nar\/30.1.325","article-title":"PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences","volume":"30","author":"Lescot","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5799\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T04:24:01Z","timestamp":1783484641000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":56,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ijms27135799"],"URL":"https:\/\/doi.org\/10.3390\/ijms27135799","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,26]]}}}