{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:51:13Z","timestamp":1785408673351,"version":"3.56.0"},"reference-count":148,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/000az4664","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007711","name":"BC Knowledge Development Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007711","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/01rkhv978","id-type":"ROR","asserted-by":"publisher"}]},{"name":"UBC Life Sciences Institute"},{"DOI":"10.13039\/501100000233","name":"Genome BC","doi-asserted-by":"crossref","award":["274PRO"],"award-info":[{"award-number":["274PRO"]}],"id":[{"id":"10.13039\/501100000233","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>This study aims to determine the prevalence of infection by larvae from the Anisakidae family in fish commonly consumed in the north of Chile. Then, 2968 specimens belonging to 22 different fish genera were studied. Anisakis spp. third-stage larvae were collected and used for PCR and proteomics studies. Trachurus murphyi was the most parasitized species (51.6%), whereas Scomber japonicus (21.3%) and Isacia conceptions (9.5%) were also found parasitized. PCR studies showed that the only species detected was Anisakis simplex. By LC-MS\/MS, we identified a total of 8119 peptide precursors, which correspond to 1919 proteins. Gene Ontology analysis indicated that, among molecular functions, catalytic and binding activities were the most highly expressed. Among biological processes, cellular and metabolic processes were the most highly expressed, while among cellular components, cellular anatomical entities and complex-containing proteins were the most highly detected. By in silico analyses, novel putative allergens were detected through comparative analyses with related genera. Among them, apolipophorin is proposed as a potential new allergen. These findings are of relevance for advancing the understanding of allergen\u2013host immune system interactions. Proteomics and bioinformatics studies strongly suggest that A. simplex is a mosaic of allergens whose implications for public health must be properly evaluated. Finally, a One Health approach is proposed to mitigate Anisakidae infections by integrating multisectoral prevention across human and animal interfaces while concurrently preserving aquatic ecosystem integrity.<\/jats:p>","DOI":"10.3390\/ijms27135922","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:50:37Z","timestamp":1782867037000},"page":"5922","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anisakis simplex: The Exclusive Member of Anisakidae Family Infecting Fish Consumed by Humans in Chile Is a Mosaic of Allergens"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1313-5014","authenticated-orcid":false,"given":"Juan","family":"San Francisco","sequence":"first","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"\u00c1valos","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Brito","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Montoya","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Zambrano","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicol\u00e1s","family":"Vivanco","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebasti\u00e1n","family":"Arenas","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Aliaga","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Carter","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Past\u00e9n","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bessy","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3796-720X","authenticated-orcid":false,"given":"Kyung-Mee","family":"Moon","sequence":"additional","affiliation":[{"name":"Departament of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC V3T1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6064-1876","authenticated-orcid":false,"given":"Rafael F.","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular e Sistemica de Tripanossomat\u00eddeos (Labtryp), Instituto Carlos Chagas Fiocruz (ICC-Fiocruz), Curitiba 81310-020, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard J.","family":"Foster","sequence":"additional","affiliation":[{"name":"Departament of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, BC V3T1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Molecular Parasitology Unit, Medical Technology Departament, Faculty of Health Sciences, University of Antofagasta, Antofagasta 1240000, Chile"},{"name":"Center for Immunology and Biomedical Biotechnology of Antofagasta, University of Antofagasta, Antofagasta 1240000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Adroher, F.J., Morales-Yuste, M., and Ben\u00edtez, R. (2024). Anisakiasis and Anisakidae. Pathogens, 13.","DOI":"10.3390\/pathogens13020148"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1007\/s11882-014-0455-3","article-title":"Allergic reactions to Anisakis found in fish","volume":"14","author":"Nieuwenhuizen","year":"2014","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.ijppaw.2019.04.007","article-title":"Anisakis nematodes in fish and shellfish- from infection to allergies","volume":"9","author":"Aibinu","year":"2019","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nonkovi\u0107, D., Te\u0161i\u0107, V., \u0160imat, V., Karabuva, S., Medi\u0107, A., and Hrabar, J. (2025). Anisakidae and Anisakidosis: A public health perspective. Pathogens, 14.","DOI":"10.3390\/pathogens14030217"},{"key":"ref_5","first-page":"150","article-title":"Anisakis simplex: Current knowledge","volume":"44","author":"Pravettoni","year":"2012","journal-title":"Eur. Ann. Allergy Clin. Immunol."},{"key":"ref_6","unstructured":"(2026, May 14). OPRHANET. Available online: https:\/\/www.orpha.net\/orphacom\/cahiers\/docs\/GB\/Prevalence_of_rare_diseases_by_alphabetical_list.pdf."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Biological Hazards (BIOHAZ) (2010). Scientific Opinion on risk assessment of parasites in fishery products. EFSA J., 8, 1543.","DOI":"10.2903\/j.efsa.2010.1543"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1016\/j.ijpara.2005.07.013","article-title":"Fish-borne parasitic zoonoses: Status and issues","volume":"35","author":"Chai","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1093\/cid\/ciy853","article-title":"Epidemiological scenario of anisakidosis in Spain based on associated hospitalizations: The tip of the iceberg","volume":"69","author":"Herrador","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.actatropica.2005.11.007","article-title":"Sensitization to Anisakis simplex s.l. in a healthy population","volume":"97","author":"Valero","year":"2006","journal-title":"Acta Trop."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/S1081-1206(10)62278-8","article-title":"Incidence of sensitivity to Anisakis simplex in a risk population of fishermen\/fishmongers","volume":"84","author":"Pastorello","year":"2000","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3585","DOI":"10.1007\/s00436-020-06892-0","article-title":"World-wide prevalence of Anisakis larvae in fish and its relationship to human allergic anisakiasis: A systematic review","volume":"119","author":"Rahmati","year":"2020","journal-title":"Parasitol. Res."},{"key":"ref_13","first-page":"203","article-title":"Anisakiasis","volume":"33","author":"Thielp","year":"1964","journal-title":"Acta Leiden."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.rvsc.2020.08.003","article-title":"Anisakiasis and Anisakis: An underdiagnosed emerging disease and its main etiological agents","volume":"132","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/bs.apar.2017.12.001","article-title":"Molecular epidemiology of Anisakis and Anisakiasis: An ecological and evolutionary road map","volume":"99","author":"Mattiucci","year":"2018","journal-title":"Adv. Parasitol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1086\/656238","article-title":"Anisakidosis: Perils of the deep","volume":"51","author":"Hochberg","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"ref_17","first-page":"297","article-title":"Biology, epidemiology, clinical features, diagnosis, and treatment of selected fish-borne parasitic zoonoses","volume":"94","author":"Cong","year":"2021","journal-title":"Yale J. Biol. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1111\/j.1572-0241.1998.00356.x","article-title":"Anisakidosis: A cause of intestinal obstruction from eating sushi","volume":"93","author":"Takabe","year":"1998","journal-title":"Am. J. Gastroenterol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1023\/B:DDAS.0000034555.55739.3c","article-title":"A case of acute gastric anisakiasis presenting with malignant tumor-like features: A large gastric vanishing tumor accompanied by local lymph node swelling","volume":"49","author":"Hiramatsu","year":"2004","journal-title":"Dig. Dis. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"270","DOI":"10.4174\/astr.2014.86.5.270","article-title":"Chronic gastric anisakiasis provoking a bleeding gastric ulcer","volume":"86","author":"Kang","year":"2014","journal-title":"Ann. Surg. Treat. Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"292","DOI":"10.3347\/PHD.23055","article-title":"A case of hepatic anisakidosis caused by Anisakis pegreffii mimicking liver cancer","volume":"61","author":"Yamada","year":"2023","journal-title":"Parasites Hosts Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3347\/kjp.1997.35.1.63","article-title":"A case of extragastrointestinal anisakiasis involving a mesocolic lymph node","volume":"35","author":"Kim","year":"1997","journal-title":"Korean J. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"598","DOI":"10.3748\/wjg.v20.i2.598","article-title":"Intestinal anisakiasis treated successfully with conservative therapy: Importance of clinical diagnosis","volume":"20","author":"Shrestha","year":"2014","journal-title":"World J. Gastroenterol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.dld.2004.06.018","article-title":"Acute abdomen due to small bowel anisakiasis","volume":"37","author":"Pellegrini","year":"2005","journal-title":"Dig. Liver Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s00403-012-1206-8","article-title":"Ani s 1 and Ani s 7 recombinant allergens are able to differentiate distinct Anisakis simplex-associated allergic clinical disorders","volume":"304","author":"Daschner","year":"2012","journal-title":"Arch. Dermatol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Mladineo, I., Popovi\u0107, M., Drmi\u0107-Hofman, I., and Poljak, V. (2016). A case report of Anisakis pegreffii (Nematoda, Anisakidae) identified from archival paraffin sections of a Croatian patient. BMC Infect. Dis., 16.","DOI":"10.1186\/s12879-016-1401-x"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Mattiucci, S., Paoletti, M., Borrini, F., Palumbo, M., Palmieri, R.M., Gomes, V., Casati, A., and Nascetti, G. (2011). First molecular identification of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae) in a paraffin-embedded granuloma taken from a case of human intestinal anisakiasis in Italy. BMC Infect. Dis., 11.","DOI":"10.1186\/1471-2334-11-82"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"111494","DOI":"10.1016\/j.molbiopara.2022.111494","article-title":"Anisakis spp, DNA detection in paraffin-embedded tissue biopsies recovered from patients with gastritis using real-time PCR in Bushehr, Persian Gulf, Iran","volume":"251","author":"Najjari","year":"2022","journal-title":"Mol. Biochem Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Roca-Geron\u00e8s, X., Alcover, M.M., God\u00ednez-Gonz\u00e1lez, C., Gonz\u00e1lez-Moreno, O., Masachs, M., Fisa, R., and Montoliu, I. (2020). First Molecular Diagnosis of Clinical Cases of Gastric Anisakiosis in Spain. Genes, 11.","DOI":"10.3390\/genes11040452"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1017\/S0022149X07820220","article-title":"Western blot antibody determination in sera from patients diagnosed with Anisakis sensitization with different antigenic fractions of Anisakis simplex purified by affinity chromatography","volume":"81","author":"Rodero","year":"2007","journal-title":"J. Helminthol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/S0091-6749(95)70301-2","article-title":"Recurrent anaphylaxis caused by Anisakis simplex parasitizing fish","volume":"96","author":"Audicana","year":"1995","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s11882-017-0698-x","article-title":"New perspectives on the diagnosis of allergy to Anisakis spp.","volume":"17","author":"Moneo","year":"2017","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/S1471-4922(01)02152-3","article-title":"Anisakis simplex: Dangerous\u2014Dead and alive?","volume":"18","author":"Audicana","year":"2002","journal-title":"Trends Parasitol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1645\/19-75","article-title":"Proteomic profiling reveals new insights into the allergomes of Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum","volume":"106","author":"Kochanowski","year":"2020","journal-title":"J. Parasitol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4127","DOI":"10.1038\/s41598-022-08113-3","article-title":"Targeted proteomics and specific immunoassays reveal the presence of shared allergens between the zoonotic nematodes Anisakis simplex and Pseudoterranova decipiens","volume":"12","author":"Saelens","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.mcp.2014.02.003","article-title":"Foodborne anisakiasis and allergy","volume":"28","author":"Baird","year":"2014","journal-title":"Mol. Cell. Probes"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Biological Hazards (BIOHAZ), Koutsoumanis, K., Allende, A., Alvarez-Ord\u00f3\u00f1ez, A., Bover-Cid, S., Chemaly, M., De Cesare, A., Herman, L., Hilbert, F., and Lindqvist, R. (2024). Re-evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 1: ToRs1\u20133. EFSA J., 22, e8719.","DOI":"10.2903\/j.efsa.2024.8719"},{"key":"ref_38","first-page":"79","article-title":"Anisakiasis humana en Chile","volume":"31","author":"Sapunar","year":"1976","journal-title":"Bol. Chil. Parasitol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1590\/S0074-02762001000500010","article-title":"Human infection by Pseudoterranova decipiens (Nematoda, Anisakidae) in Chile: Report of seven cases","volume":"96","author":"Mercado","year":"2001","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1590\/S0034-89101997000200012","article-title":"Human case of gastric infection by a fourth larval stage of Pseudoterranova decipiens (Nematoda, Anisakidae)","volume":"31","author":"Mercado","year":"1997","journal-title":"Rev. Saude Publica"},{"key":"ref_41","unstructured":"World Health Organization (2026, May 14). Available online: https:\/\/www.who.int\/health-topics\/one-health#tab=tab_1."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1002\/prot.20264","article-title":"Scoring function for automated assessment of protein structure template quality","volume":"57","author":"Zhang","year":"2004","journal-title":"Proteins"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1093\/bioinformatics\/btq066","article-title":"How significant is a protein structure similarity with TM-score = 0.5?","volume":"26","author":"Xu","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_44","first-page":"591","article-title":"Diagnosis of a case of gastric anisakidosis by PCR-based restriction fragment length polymorphism analysis","volume":"41","author":"Mathiopoulos","year":"1999","journal-title":"Parassitologia"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"496","DOI":"10.3201\/eid1903.121017","article-title":"Anisakiasis and gastroallergic reactions associated with Anisakis pegreffii infection, Italy","volume":"19","author":"Mattiucci","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Mattiucci, S., Paoletti, M., Colantoni, A., Carbone, A., Gaeta, R., Proietti, A., Frattaroli, S., Fazii, P., Bruschi, F., and Nascetti, G. (2017). Invasive anisakiasis by the parasite Anisakis pegreffii (Nematoda: Anisakidae): Diagnosis by real-time PCR hydrolysis probe system and immunoblotting assay. BMC Infect. Dis., 17.","DOI":"10.1186\/s12879-017-2633-0"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"De Benedetto, G., Giannetto, A., Riolo, K., Iaria, C., Brianti, E., and Gaglio, G. (2021). Anisakis pegreffii larvae in sphyraena viridensis and description of granulomatous lesions. Animals, 11.","DOI":"10.3390\/ani11123449"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.3201\/eid2110.141848","article-title":"Human infections with Pseudoterranova cattani nematodes, Chile","volume":"21","author":"Weitzel","year":"2015","journal-title":"Emerg. Infect. Dis."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1007\/s00436-022-07459-x","article-title":"Prevalence and molecular identification of zoonotic Anisakis and Pseudoterranova species in fish destined to human consumption in Chile","volume":"121","author":"Machuca","year":"2022","journal-title":"Parasitol. Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1016\/S0140-6736(96)91648-7","article-title":"Gastric anisakidosis due to Pseudoterranova decipiens larva","volume":"347","author":"Pinel","year":"1996","journal-title":"Lancet"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.3201\/eid2806.212483","article-title":"Molecular diagnosis of Pseudoterranova decipiens sensu stricto infections, South Korea, 2002\u20132020","volume":"28","author":"Song","year":"2022","journal-title":"Emerg. Infect. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.4315\/0362-028X.JFP-13-538","article-title":"Anisakid parasites in commercial hake ceviche in southern chile","volume":"77","author":"Torres","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1111\/jfd.13369","article-title":"Increasing intensities of Anisakis simplex (Rudolphi, 1809 det. Krabbe, 1878) larvae with weight and sea age in returning adult Atlantic salmon, Salmo salar L., of coastal waters of Norway","volume":"44","author":"Mo","year":"2021","journal-title":"J. Fish. Dis."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3417","DOI":"10.1007\/s11033-021-06323-y","article-title":"Prevalence and identification of Anisakis nematodes in fish consumed in Marrakesh, Morocco","volume":"48","author":"Biary","year":"2021","journal-title":"Mol. Biol. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Alburqueque, R.A., Palomba, M., Santoro, M., and Mattiucci, S. (2020). Molecular identification of zoonotic parasites of the genus anisakis (Nematoda: Anisakidae) from fish of the southeastern Pacific Ocean (off Peru coast). Pathogens, 9.","DOI":"10.3390\/pathogens9110910"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1051\/parasite\/1999063223","article-title":"Metazoan parasites of the Jack Mackerel Trachurus murphyi (Teleostei, Carangidae) in a latitudinal gradient from South America (Chile and Peru)","volume":"6","author":"Oliva","year":"1999","journal-title":"Parasite"},{"key":"ref_57","first-page":"71","article-title":"The presence of Anisakis sp. larvae in the Pacific jack mackerel, Trachurus murphyi, Nichols, 1920. (some comments on their relation with human eosinophilic granuloma) (author\u2019s transl)","volume":"31","author":"Cattan","year":"1976","journal-title":"Bol. Chil. Parasitol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"110181","DOI":"10.1016\/j.ijfoodmicro.2023.110181","article-title":"Prevalence and abundance of Anisakis larvae in ready-to-eat mackerel products in Japan","volume":"395","author":"Ohnishi","year":"2023","journal-title":"Int. J. Food Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"110347","DOI":"10.1016\/j.ijfoodmicro.2023.110347","article-title":"Prevalence of Anisakis larvae in cultured mackerel Scomber japonicas in Japan and the relationship between the intensity of Anisakis infection in cultured mackerel and fish fatness","volume":"404","author":"Kodo","year":"2023","journal-title":"Int. J. Food Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Martin-Carrillo, N., Garc\u00eda-Livia, K., Baz-Gonz\u00e1lez, E., Abreu-Acosta, N., Dorta-Guerra, R., Valladares, B., and Foronda, P. (2022). Morphological and Molecular Identification of Anisakis spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data. Animals, 12.","DOI":"10.3390\/ani12192634"},{"key":"ref_61","first-page":"200","article-title":"Pseudoterranovosis and sushi","volume":"25","author":"Neira","year":"2008","journal-title":"Rev. Chil. Infectol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1177\/039463200501800408","article-title":"Cross-reactivity between IgE-binding proteins from Anisakis simplex and Dermatophagoides pteronyssinus","volume":"18","author":"Bernardini","year":"2005","journal-title":"Int. J. Immunopathol. Pharmacol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1051\/parasite\/2004112219","article-title":"Cross-reactivity between antigens of Anisakis simplex s.l. and other ascarid nematodes","volume":"11","author":"Lozano","year":"2004","journal-title":"Parasite"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1079\/JOH2003196","article-title":"Cross-reactivity induced by Anisakis simplex and Toxocara canis in mice","volume":"77","author":"Perteguer","year":"2003","journal-title":"J. Helminthol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1007\/s004360050131","article-title":"Antigenic cross-reactivity in mice between third-stage larvae of Anisakis simplex and other nematodes","volume":"82","author":"Iglesias","year":"1996","journal-title":"Parasitol. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.chroma.2016.01.002","article-title":"Development of liquid chromatography-tandem mass spectrometry methods for the quantitation of Anisakis simplex proteins in fish","volume":"1432","author":"Moen","year":"2016","journal-title":"J. Chromatogr. A"},{"key":"ref_67","first-page":"1","article-title":"Proteome profiling of L3 and L4 Anisakis simplex development stages by TMT-based quantitative proteomics","volume":"201","author":"Mateos","year":"2019","journal-title":"J. Proteom."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.jprot.2016.05.012","article-title":"Protein biomarker discovery and fast monitoring for the identification and detection of Anisakids by parallel reaction monitoring (PRM) mass spectrometry","volume":"142","author":"Carrera","year":"2016","journal-title":"J. Proteom."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Palomba, M., Rughetti, A., Mignogna, G., Castrignan\u00f2, T., Rahimi, H., Masuelli, L., Napoletano, C., Pinna, V., Giorgi, A., and Santoro, M. (2023). Proteomic characterization of extracellular vesicles released by third stage larvae of the zoonotic parasite Anisakis pegreffii (Nematoda: Anisakidae). Front. Cell Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1079991"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"625","DOI":"10.2307\/3282971","article-title":"Identification of the secreted neutral proteases from Anisakis simplex","volume":"76","author":"Sakanari","year":"1990","journal-title":"J. Parasitol."},{"key":"ref_71","unstructured":"Erzinger, G.S. (2012). Peptidases in parasitic nematodes: A review. Parasites: Ecology, Diseases and Management, Nova Science Publisher Inc."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Palomba, M., Cipriani, P., Giulietti, L., Levsen, A., Nascetti, G., and Mattiucci, S. (2020). Differences in gene expression profiles of seven target proteins in third-stage larvae of Anisakis simplex (Sensu stricto) by sites of infection in blue whiting (micromesistius poutassou). Genes, 11.","DOI":"10.3390\/genes11050559"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.molbiopara.2018.10.002","article-title":"Comparative transcriptome analyses of the third and fourth stage larvae of Anisakis simplex (Nematoda: Anisakidae)","volume":"226","author":"Kim","year":"2018","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Robinson, M.W., and Dalton, J.P. (2011). Cysteine Proteases of Pathogenics Organisms, Springer Nature.","DOI":"10.1007\/978-1-4419-8414-2"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1089\/fpd.2019.2633","article-title":"Differential cleaving of specific substrates for cathepsin-like activity shows cysteine and serine protease activities and a differential profile between Anisakis simplex s.s. and Anisakis pegreffii, sibling species major etiologic agents of Anisakiasis","volume":"16","author":"Adroher","year":"2019","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.actatropica.2019.04.003","article-title":"Differential proteolytic activity in Anisakis simplex s.s. and Anisakis pegreffii, two sibling species from the complex Anisakis simplex s.l., major etiological agents of Anisakiasis","volume":"195","author":"Adroher","year":"2019","journal-title":"Acta Trop."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1128\/iai.64.4.1328-1334.1996","article-title":"Characterization of the cathepsin-like cysteine proteinases of Schistosoma mansoni","volume":"64","author":"Dalton","year":"1996","journal-title":"Infect. Immun."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1645\/0022-3395(2000)086[0711:ILOIFI]2.0.CO;2","article-title":"Immunofluorescent localization of intermediate filaments (IFs) in helminths using anti-mammalian Ifs monoclonal antibody","volume":"86","author":"Sato","year":"2000","journal-title":"J. Parasitol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"4633","DOI":"10.1002\/pmic.200600068","article-title":"Plasticity demonstrated in the proteome of a parasitic nematode within the intestine of different host strains","volume":"6","author":"Morgan","year":"2006","journal-title":"Proteomics"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"189","DOI":"10.3347\/kjp.2021.59.3.189","article-title":"Albendazole and mebendazole as anti-parasitic and anti-cancer agents: An update","volume":"59","author":"Chai","year":"2021","journal-title":"Korean J. Parasitol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1146\/annurev-biochem-080111-092106","article-title":"The clothes make the mRNA: Past and present trends in mRNP fashion","volume":"84","author":"Singh","year":"2015","journal-title":"Annu. Rev. Biochem."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.molcel.2014.04.033","article-title":"Principles and properties of Eukaryotic mRNPs","volume":"54","author":"Mitchell","year":"2014","journal-title":"Mol. Cell"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1764","DOI":"10.1016\/j.molcel.2024.03.012","article-title":"Time-resolved profiling of RNA binding proteins throughout the mRNA life cycle","volume":"84","author":"Choi","year":"2024","journal-title":"Mol. Cell"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Rios-Castro, R., Abollo, E., and Pascual, S. (2026). Potential impact of marine heatwaves on the survival and transcriptomic signature of free-living third-stage larvae (L3) of Anisakis simplex (Nematoda: Anisakidae). Front. Vet. Sci., 13.","DOI":"10.3389\/fvets.2026.1758357"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13071-017-2585-7","article-title":"Tissue-specific transcriptomes of Anisakis simplex (sensu stricto) and Anisakis pegreffii reveal potential molecular mechanisms involved in pathogenicity","volume":"11","author":"Cavallero","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1186\/s13071-025-07197-w","article-title":"Polymorphism-driven transcriptomic changes in anthelmintic metabolism pathways of Anisakis simplex s.s. L3 larvae","volume":"18","author":"Polak","year":"2025","journal-title":"Parasites Vectors"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"108751","DOI":"10.1016\/j.exppara.2024.108751","article-title":"The response of Anisakis simplex (s. s.) to anthelmintic Specific changes in xenobiotic metabolic processes","volume":"261","author":"Polak","year":"2024","journal-title":"Exp. Parasitol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1007\/s12192-015-0607-y","article-title":"The role of small heat shock proteins in parasites","volume":"20","author":"Espinoza","year":"2015","journal-title":"Cell Stress. Chaperones"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/imcb.12171","article-title":"Immunobiology of parasitic worm extracellular vesicles","volume":"96","author":"Eichenberger","year":"2018","journal-title":"Immunol. Cell Biol."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"Eichenberger, R.M., Ryan, S., Jones, L., Buitrago, G., Polster, R., de Oca, M.M., Zuvelek, J., Giacomin, P.R., Dent, L.A., and Engwerda, C.R. (2018). Hookworm secreted extracellular vesicles interact with host cells and prevent inducible colitis in mice. Front. Immunol., 9.","DOI":"10.3389\/fimmu.2018.00850"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1038\/337620a0","article-title":"Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria","volume":"337","author":"Cheng","year":"1989","journal-title":"Nature"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1146\/annurev.bb.21.060192.001453","article-title":"Protein folding in the cell: The role of molecular chaperones Hsp70 and Hsp60","volume":"21","author":"Hartl","year":"1992","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2958.1995.tb02216.x","article-title":"Evolution of the chaperonin families (HSP60, HSP 10 and TCP-1) of proteins and the origin of eukaryotic cells","volume":"15","author":"Gupta","year":"1995","journal-title":"Mol. Microbiol."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/S0959-437X(99)00030-1","article-title":"Evolution of organellar genomes","volume":"9","author":"Gray","year":"1999","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1146\/annurev.cb.09.110193.003125","article-title":"Role of the major heat shock proteins as molecular chaperones","volume":"9","author":"Georgopoulos","year":"1993","journal-title":"Annu. Rev. Cell Biol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/S0959-440X(99)80013-X","article-title":"Principles of protein folding in the cellular environment","volume":"9","author":"Ellis","year":"1999","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_97","first-page":"92","article-title":"Molecular chaperones in cellular protein folding","volume":"5","author":"Hartl","year":"1996","journal-title":"Nature"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/0968-0004(94)90281-X","article-title":"DnaJ-like proteins: Molecular chaperones and specific regulators of Hsp70","volume":"19","author":"Cyr","year":"1994","journal-title":"Trends Biochem. Sci."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1091\/mbc.4.6.555","article-title":"Eukaryotic homologues of Escherichia coli dnaJ: A diverse protein family that functions with HSP70 stress proteins","volume":"4","author":"Caplan","year":"1993","journal-title":"Mol. Biol. Cell"},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1021\/bi001659k","article-title":"The ubiquitin-proteasome pathwayplays an essential role in proteolysis during Trypanosoma cruzi remodeling","volume":"40","author":"Katz","year":"2001","journal-title":"Biochemistry"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1046\/j.1432-1327.1999.00682.x","article-title":"Proteasome-dependent cyst formation and stage-specific ubiquitin mRNA accumulation in Entamoeba invadens","volume":"264","author":"Gonzalez","year":"1999","journal-title":"Eur. J. Biochem."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1084\/jem.184.5.1909","article-title":"Proteasome activity is required for the stage-specific transformation of a protozoan parasite","volume":"184","author":"Frevert","year":"1996","journal-title":"J. Exp. Med."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1007\/s00436-015-4360-z","article-title":"Proteasome stress responses in Schistosoma mansoni","volume":"114","author":"Morais","year":"2015","journal-title":"Parasitol. Res."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1007\/s00436-009-1430-0","article-title":"The ubiquitin-proteasome system in Strongyloididae. Biochemical evidence for developmentally regulated proteolysis in Strongyloides venezuelensis","volume":"105","author":"Ueta","year":"2009","journal-title":"Parasitol. Res."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.exppara.2005.01.002","article-title":"Schistosoma mansoni: Functional proteasomes are required for development in the vertebrate host","volume":"109","author":"Evangelista","year":"2005","journal-title":"Exp. Parasitol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1002\/pmic.201300529","article-title":"Proteomic profiling and characterization of differential allergens in the nematodes Anisakis simplex sensu stricto and A. pegreffii","volume":"14","author":"Arcos","year":"2014","journal-title":"Proteomics"},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"e2020-41","DOI":"10.21307\/jofnem-2020-041","article-title":"De novo transcriptome sequencing and analysis of Anisakis pegreffii (Nematoda: Anisakidae) third-stage and fourth stage larvae","volume":"52","author":"Nam","year":"2020","journal-title":"J. Nematol."},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Polak, I., Stryi\u0144ski, R., Ma\u017adziarz, M., Paukszto, L., Carrera, M., Bogacka, I., and \u0141opie\u0144ska-Biernat, E. (2025). Coupled transcriptome and proteome analysis of L3 and L4 developmental stages of Anisakis simplex s. s.: Insights into target genes under glucose influence. BMC Genom., 26.","DOI":"10.1186\/s12864-025-12068-w"},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Marzano, V., Pane, S., Foglietta, G., Mortera, S.L., Vernocchi, P., Muda, A.O., and Putignani, L. (2020). Mass spectrometry based-proteomic analysis of Anisakis spp.: A preliminary study towards a new diagnostic tool. Genes, 11.","DOI":"10.3390\/genes11060693"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1159\/000444981","article-title":"Ani s 11-Like Protein Is a Pepsin- and Heat-Resistant Major Allergen of Anisakis spp. and a Valuable Tool for Anisakis Allergy Component-Resolved Diagnosis","volume":"169","author":"Careche","year":"2016","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"605","DOI":"10.4315\/0362-028X.JFP-13-278","article-title":"Identification of autoclave-resistant Anisakis simplex allergens","volume":"77","author":"Olivares","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.molbiopara.2008.02.008","article-title":"Cloning and expression of Ani s 9, a new Anisakis simplex allergen","volume":"159","author":"Moneo","year":"2008","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.euprot.2014.06.006","article-title":"Characterisation of potential novel allergens in the fish parasite Anisakis simplex","volume":"4","author":"Jonscher","year":"2014","journal-title":"EuPA Open Proteom."},{"key":"ref_114","doi-asserted-by":"crossref","unstructured":"International Helminth Genomes Consortium (2019). Comparative genomics of the major parasitic worms International Helminth Genomes Consortium. Nat. Genet., 51, 163\u2013174.","DOI":"10.1038\/s41588-018-0262-1"},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1128\/CMR.00012-07","article-title":"Anisakis simplex: From obscure infectious worm to inducer of immune hypersensitivity","volume":"21","author":"Audicana","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"101062","DOI":"10.1016\/j.waojou.2025.101062","article-title":"Risk factors\/cofactors for heightened anaphylaxis severity in Japanese adults: A 10-year single-center retrospective cohort study","volume":"18","author":"Nojo","year":"2025","journal-title":"World Allergy Organ. J."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1016\/j.jaci.2005.12.1357","article-title":"Exposure to the fish parasite Anisakis causes allergic airway hyperreactivity and dermatitis","volume":"117","author":"Nieuwenhuizen","year":"2006","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_118","doi-asserted-by":"crossref","unstructured":"D\u2019amelio, S., Lombardo, F., Pizzarelli, A., Bellini, I., and Cavallero, S. (2020). Advances in omic studies drive discoveries in the biology of anisakid nematodes. Genes, 11.","DOI":"10.3390\/genes11070801"},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1365-4632.2006.03091.x","article-title":"Cross-reactivity of Anisakis simplex: Possible role of Ani s 2 and Ani s 3","volume":"46","author":"Guarneri","year":"2007","journal-title":"Int. J. Dermatol."},{"key":"ref_120","doi-asserted-by":"crossref","unstructured":"Jeron\u010di\u0107, A., Nonkovi\u0107, D., Vrbatovi\u0107, A., Hrabar, J., Bu\u0161eli\u0107, I., Mart\u00ednez-Sern\u00e1ndez, V., Rocomonde, S.A.L., Ubeira, F.M., Jaman, S., and Jeli\u010di\u0107, E.C. (2020). Anisakis sensitization in the Croatian fish processing workers: Behavioral instead of occupational risk factors?. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008038"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.ijpara.2015.01.002","article-title":"Haemoglobin, a new major allergen of Anisakis simplex","volume":"45","author":"Daschner","year":"2015","journal-title":"Int. J. Parasitol."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/j.1398-9995.2007.01564.x","article-title":"Novel sequences and epitopes of diagnostic value derived from the Anisakis simplex Ani s 7 major allergen","volume":"63","author":"Iglesias","year":"2008","journal-title":"Allergy"},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Mayoral, M.F., Trevi\u00f1o, M.A., P\u00e9rez-Pi\u00f1ar, T., Caballero, M.L., Knaute, T., Umpierrez, A., Briux, M., and Rodriguez-Perez, R. (2014). Relationships between IgE\/IgG4 Epitopes, Structure and Function in Anisakis simplex Ani s 5, a Member of the SXP\/RAL-2 Protein Family. PLoS Negl. Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002735"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.molbiopara.2010.07.005","article-title":"Elucidation of IgE-binding epitopes of Ani s 1: The major Anisakis simplex allergen","volume":"174","author":"Kobayashi","year":"2010","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1159\/000024266","article-title":"High-molecular-weight allergens of the house dust mite: An apolipophorin-like cDNA has sequence identity with the major M-177 allergen and the IgE-binding peptide fragments Mag1 and Mag3","volume":"120","author":"Epton","year":"1999","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S1389-1723(02)80166-9","article-title":"Toward elucidating the full spectrum of mite allergens\u2014State of the art","volume":"94","author":"Kawamoto","year":"2002","journal-title":"J. Biosci. Bioeng."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1603\/ME10028","article-title":"Identification and expression profile of multiple genes in response to magnesium exposure in Culex quinquefasciatus larvae","volume":"47","author":"Zhao","year":"2010","journal-title":"J. Med. Entomol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"e2300811","DOI":"10.1002\/mnfr.202300811","article-title":"The Allergen Profile of Two Edible Insect Species\u2014Acheta domesticus and Hermetia illucens","volume":"68","author":"Karnaneedi","year":"2024","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_129","doi-asserted-by":"crossref","unstructured":"Barre, A., Pichereaux, C., Simplicien, M., Burlet-Schiltz, O., Benoist, H., and Roug\u00e9, P. (2021). A proteomic-and bioinformatic-based identification of specific allergens from edible insects: Probes for future detection as food ingredients. Foods, 10.","DOI":"10.3390\/foods10020280"},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"100062","DOI":"10.1016\/j.toxcx.2020.100062","article-title":"Partial proteomic analysis of brown widow spider (Latrodectus geometricus) venom to determine the biological activities","volume":"8","author":"Khamtorn","year":"2020","journal-title":"Toxicon X"},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13028-017-0355-3","article-title":"Food safety challenges and One Health within Europe","volume":"60","author":"Boqvist","year":"2018","journal-title":"Acta Vet. Scand."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"316","DOI":"10.4315\/0362-028X.JFP-10-336","article-title":"Liquid chromatography and mass spectrometry in food allergen detection","volume":"74","author":"Christians","year":"2011","journal-title":"J. Food Prot."},{"key":"ref_133","first-page":"e31949","article-title":"The utilization of activated charcoal in the management of anaphylaxis: A Case Series","volume":"14","author":"Duyan","year":"2022","journal-title":"Cureus"},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1021\/jf102984z","article-title":"Release of soluble protein from peanut (Arachis hypogaea, Leguminosae) and its adsorption by activated charcoal","volume":"59","author":"Kopper","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"10619","DOI":"10.1021\/jf801731n","article-title":"Adsorption of peanut (Arachis hypogaea, Leguminosae) proteins by activated charcoal","volume":"56","author":"Kopper","year":"2008","journal-title":"J. Agric. Food Chem."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.ijppaw.2017.08.001","article-title":"Anisakidae nematodes isolated from the flathead grey mullet fish (Mugil cephalus) of Buenaventura, Colombia","volume":"6","author":"Castellanos","year":"2017","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0020-7519(99)00178-2","article-title":"Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase-chain-reaction based restriction fragment length polymorphism","volume":"30","author":"Mathiopoulos","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"3704","DOI":"10.1021\/acs.jproteome.8b00343","article-title":"In-depth quantitative proteomic analysis of trophozoites and pseudocysts of Trichomonas vaginalis","volume":"17","author":"Vidal","year":"2018","journal-title":"J. Proteome Res."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1080\/21505594.2022.2132776","article-title":"Trypanosoma cruzi pathogenicity involves virulence factor expression and upregulation of bioenergetic and biosynthetic pathways","volume":"13","author":"Astudillo","year":"2022","journal-title":"Virulence"},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/pro.4218","article-title":"PANTHER: Making genome-scale phylogenetic accessible to all","volume":"31","author":"Thomas","year":"2022","journal-title":"Protein Sci."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"W216","DOI":"10.1093\/nar\/gkac194","article-title":"DAVID: A web server for functional enrichment analysis and functional annotation of gene lists (2021 update)","volume":"50","author":"Sherman","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1093\/bioinformatics\/btt619","article-title":"AllergenFP: Allergenicity prediction by descriptor fingerprints","volume":"30","author":"Dimitrov","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"bbaa294","DOI":"10.1093\/bib\/bbaa294","article-title":"AlgPred 2.0: An improved method for predicting allergenic proteins and mapping of IgE epitopes","volume":"22","author":"Sharma","year":"2021","journal-title":"Brief. Bioinform."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"11486","DOI":"10.1074\/jbc.RA120.014016","article-title":"T cell receptor interactions with human leukocyte antigen govern indirect peptide selectivity for the cancer testis antigen MAGE-A4","volume":"295","author":"Coles","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"W215","DOI":"10.1093\/nar\/gkae237","article-title":"DeepLoc 2.1: Multi-label membrane protein type prediction using protein language models","volume":"52","author":"Teufel","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"eadj6367","DOI":"10.1126\/sciadv.adj6367","article-title":"Accurate prediction of HLA class II antigen presentation across all loci using tailored data acquisition and refined machine learning","volume":"9","author":"Nilsson","year":"2023","journal-title":"Sci. Adv."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5922\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T04:31:10Z","timestamp":1783053070000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/13\/5922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,30]]},"references-count":148,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ijms27135922"],"URL":"https:\/\/doi.org\/10.3390\/ijms27135922","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,30]]}}}