{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T12:21:16Z","timestamp":1783081276033,"version":"3.54.6"},"reference-count":97,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The bacterial and toxin methods of cancer treatment date back 130 years. This paradigm rests on nonspecific bacterial-toxin-generated immunotherapy. This high-risk oncology research is experiencing a renaissance of methods that are among the most effective yet. Glioblastomas and other resistant cancers are the modern touchpoint, because of remission history following sepsis. Spurred by recent research deaths, we discuss protocols IRBs should consider in live bacterial or synthetic immuno-stimulatory trials. Human systemic inflammatory response syndrome immunology is unique due to non-functioning SIGLEC-13 and 17, which control excessive Toll-like receptor 4 (TLR-4) signaling. This is not a technicality like human CD8+\/CD4+ T cells. SIGLEC-13&amp;17 consequences are profound; humans are \u2248330\u2013200,000 times more sensitive to LPS\/endotoxin than mice and rats. This human TLR-4 difference also applies to gene therapy and should inform the results from any animal model, including non-human primates. Clinical TLR-4 stimulation takes two forms: bacterial infection and sterile TLR-4 stimulators, and treatments differ. The stereotactic injection of calculated amounts of adjuvants like endotoxin, venoms\/components, or synthetic alternatives may be safer than live bacteria. Inadequate planning for risk elements, basic predictive models, and treatments will likely cause death.<\/jats:p>","DOI":"10.3390\/ijms27135985","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T11:39:11Z","timestamp":1783078751000},"page":"5985","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Perspective on Lessons Not Learned: From Coley\u2019s Toxins to Microbial Drug Delivery, Guidance for Institutional Review Boards (IRBs)"],"prefix":"10.3390","volume":"27","author":[{"given":"Brian P.","family":"Hanley","sequence":"first","affiliation":[{"name":"UTRGV Department of Surgery, UTRGV School of Medicine, 1210 W. Schunior St., Edinburg, TX 78541, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro J.","family":"Betancourt","sequence":"additional","affiliation":[{"name":"UTRGV Department of Surgery, UTRGV School of Medicine, 1210 W. Schunior St., Edinburg, TX 78541, USA"},{"name":"Neurosurgery, Valley Baptist Medical Center, 1040 W. Jefferson St., Brownsville, TX 78520, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Gross","sequence":"additional","affiliation":[{"name":"UTRGV Department of Surgery, UTRGV School of Medicine, 1210 W. Schunior St., Edinburg, TX 78541, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilbur (Bo)","family":"Bowne","sequence":"additional","affiliation":[{"name":"UTRGV Department of Surgery, UTRGV School of Medicine, 1210 W. Schunior St., Edinburg, TX 78541, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Meng, Y., Sun, J., Zhang, G., Yu, T., and Piao, H. (2023). Bacteria associated with glioma: A next wave in cancer treatment. Front. Cell. Infect. 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