{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:47:08Z","timestamp":1785422828736,"version":"3.56.0"},"reference-count":46,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Walailak University Graduate Scholarship and the Graduate Research Fund","award":["26\/2024"],"award-info":[{"award-number":["26\/2024"]}]},{"name":"Walailak University Graduate Scholarship and the Graduate Research Fund","award":["CGS-RF-2025\/29"],"award-info":[{"award-number":["CGS-RF-2025\/29"]}]},{"award":["26\/2024"],"award-info":[{"award-number":["26\/2024"]}],"id":[{"id":"https:\/\/ror.org\/04b69g067","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CGS-RF-2025\/29"],"award-info":[{"award-number":["CGS-RF-2025\/29"]}],"id":[{"id":"https:\/\/ror.org\/04b69g067","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>ESKAPE pathogens have become a major global health challenge. This study aimed to isolate and characterize LAB from fermented cacao and to evaluate their probiotic properties, preliminary antimicrobial activity against ESKAPE pathogens, safety profiles, and functional bioactivities. Each of P. acidilactici isolates exhibited antibacterial activity against ESKAPE pathogens, with inhibition zone diameters ranging from 10.00 \u00b1 1.00 mm to 23.00 \u00b1 0.00 mm, depending on the isolate and pathogens tested. CR05 was identified as the most promising probiotic candidate, showing the highest survival at pH 2 (69.65 \u00b1 6.66%), strong tolerance to pancreatin (99.95 \u00b1 0.10%), pepsin (89.11 \u00b1 2.38%), bile salts (98.65 \u00b1 0.33%), and favorable adhesion properties, including auto-aggregation, cell surface hydrophobicity, and adhesion to HT-29 intestinal epithelial cells. The safety assessment indicated a notable susceptibility to gentamicin, tetracycline, and chloramphenicol, with resistance to several other tested antibiotics. and showed no hemolytic activity. Three selected isolates were evaluated for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against ESKAPE pathogens, with MIC and MBC values ranging from 12.5 to 25 mg\/mL and 25 to &gt;50 mg\/mL, respectively. The strongest inhibitory activity was observed against A. baumannii, P. aeruginosa, and E. aerogenes, with MIC values of 12.5 mg\/mL, particularly for isolates CR05 and CR06. Whole-genome analysis identified genes related to stress response and gastrointestinal tolerance and predicted the enterolysin A gene. No acquired antimicrobial resistance genes were detected. These findings suggest that P. acidilactici isolates from fermented cacao are promising probiotic candidates for further investigation in functional food, probiotic, and postbiotic-related applications.<\/jats:p>","DOI":"10.3390\/ijms27135996","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T14:33:06Z","timestamp":1783089186000},"page":"5996","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Genomic and Functional Characterization of Pediococcus acidilactici Isolated from Fermented Cacao with Anti-ESKAPE Activity"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3102-4462","authenticated-orcid":false,"given":"Pinkanok","family":"Suksabay","sequence":"first","affiliation":[{"name":"College of Graduate Studies, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2594-8153","authenticated-orcid":false,"given":"Yosita","family":"Leepromma","sequence":"additional","affiliation":[{"name":"College of Graduate Studies, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benyapa","family":"Prakit","sequence":"additional","affiliation":[{"name":"College of Graduate Studies, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2499-7765","authenticated-orcid":false,"given":"Tansuda","family":"Puchong","sequence":"additional","affiliation":[{"name":"College of Graduate Studies, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5074-2016","authenticated-orcid":false,"given":"Joo Shun","family":"Tan","sequence":"additional","affiliation":[{"name":"Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Gelugor 11800, Pulau Pinang, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1403-1628","authenticated-orcid":false,"given":"Chonticha","family":"Romyasamit","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Research Center for Microbiome, Systems Biology, and Medical Innovation, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Research Center in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Gruber, D., Vogel, V., Walter, J.-C., Bolotnikov, G., Rodr\u00edguez, A., Preising, N., St\u00e4ndker, L., Firacative, C., Spellerberg, B., and Kissmann, A.-K. 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