{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:40:39Z","timestamp":1785400839618,"version":"3.56.0"},"reference-count":39,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU H2020-Marie Sk\u0142odowska-Curie Actions-RISE project Translational Research on Endometriosis TRENDO","award":["101008193"],"award-info":[{"award-number":["101008193"]}]},{"name":"Federal Ministry of Education and Research (Germany) BMFTR","award":["01EJ2401A"],"award-info":[{"award-number":["01EJ2401A"]}]},{"name":"Federal Ministry of Education and Research (Germany) BMFTR","award":["01EJ2402B"],"award-info":[{"award-number":["01EJ2402B"]}]},{"award":["01EJ2401A"],"award-info":[{"award-number":["01EJ2401A"]}],"id":[{"id":"https:\/\/ror.org\/04pz7b180","id-type":"ROR","asserted-by":"publisher"}]},{"award":["01EJ2402B"],"award-info":[{"award-number":["01EJ2402B"]}],"id":[{"id":"https:\/\/ror.org\/04pz7b180","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Medical Faculty of M\u00fcnster University"},{"id":[{"id":"https:\/\/ror.org\/00pd74e08","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/012kf4317","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["544376111"],"award-info":[{"award-number":["544376111"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"award":["544376111"],"award-info":[{"award-number":["544376111"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Open Access Publication Fund of the University of M\u00fcnster"},{"id":[{"id":"https:\/\/ror.org\/00pd74e08","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Endometriosis is a disease associated with pain symptoms and reduced fertility, characterized by the presence of endometrial tissue outside the uterus. SETD3 is an actin-specific histidine N-methyltransferase that regulates actin stability and flexibility. Here, we investigate the effects of altered SETD3 expression on cytoskeletal function and endometriotic cell motility. SETD3 expression in endometriotic lesions was analyzed using EndometDB, and in uteri of the superfertile Dummerstorf mouse line FL1 by RT-qPCR. The functional impact of siRNA-mediated SETD3 depletion on endometriotic 12Z cells and primary endometriotic stroma cells was studied in vitro. Cell motility, contractility, invasiveness, morphology and gene expression were analyzed by RT-qPCR, Western blotting, immunofluorescence, scratch wound, collagen contraction and Matrigel invasion assays. In patient tissue, SETD3 expression was slightly increased in deep endometriotic lesions, whereas SETD3 was downregulated 1.6-fold in the uteri of superfertile mice. SETD3 depletion delayed cell motility, reduced invasiveness of 12Z cells, and reduced the capability to contract collagen gels. Cytoskeletal gene expression was moderately changed. Our data suggest that the histidine N-methyltransferase SETD3 contributes to cytoskeletal remodeling that plays a key role in cell migration and invasion. A dysregulation of SETD3 could therefore be related to the pathogenesis of endometriosis, particularly in deep endometriosis.<\/jats:p>","DOI":"10.3390\/ijms27136069","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T08:04:49Z","timestamp":1783411489000},"page":"6069","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Analysis of the Histidine N-Methyltransferase SETD3 in Endometriosis"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5260-1738","authenticated-orcid":false,"given":"Melanie","family":"Poloczek","sequence":"first","affiliation":[{"name":"Department of Gynecology and Obstetrics, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolin Lisa Michaela","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Biology, Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7708-7995","authenticated-orcid":false,"given":"Hanna","family":"Surmann","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Strau\u00df","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3870-7699","authenticated-orcid":false,"given":"Michael","family":"Gabriel","sequence":"additional","affiliation":[{"name":"Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, 20520 Turku, Finland"},{"name":"Department of Obstetrics and Gyneacology, Institute of Medicine, University of Turku, 20014 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matti","family":"Poutanen","sequence":"additional","affiliation":[{"name":"Research Centre for Integrative Physiology and Pharmacology, Institute of Biomedicine, University of Turku, 20520 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3963-8661","authenticated-orcid":false,"given":"Julia","family":"Oto","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 M\u00fcnster, Germany"},{"name":"Haemostasis, Thrombosis, Arteriosclerosis and Vascular Biology Research Group, Medical Research Institute Hospital La Fe, 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7247-9247","authenticated-orcid":false,"given":"Ludwig","family":"Kiesel","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8010-1463","authenticated-orcid":false,"given":"Sebastian D.","family":"Sch\u00e4fer","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 M\u00fcnster, Germany"},{"name":"Department of Gynecology and Obstetrics, Clemenshospital M\u00fcnster, 48153 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Hanker","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4073-6691","authenticated-orcid":false,"given":"Joachim M.","family":"Weitzel","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Biology, Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2360-2496","authenticated-orcid":false,"given":"Martin","family":"G\u00f6tte","sequence":"additional","affiliation":[{"name":"Department of Gynecology and Obstetrics, University Hospital Muenster, Albert-Schweitzer-Campus 1, 48149 M\u00fcnster, Germany"},{"name":"Cells-in-Motion Interfaculty Centre (CiMIC), University of M\u00fcnster, 48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1056\/NEJMra1810764","article-title":"Endometriosis","volume":"382","author":"Zondervan","year":"2020","journal-title":"N. 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