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Most effective among the screened compounds were bicyclic hydroxylactams 6\u20139 with a pyrrolo[3,4-d]isoxazoline structure; they showed IC50 values ranging from 12 to 36 \u03bcg\/mL for all tested cancer cell lines with selectivity indexes up to 12 (as compared to the embryonic kidney HEK293T cell line). Loss of stress fibers with diffuse redistribution of granular actin throughout the cytoplasm in up to 25% of treated cells and a decrease in filopodia-like protrusions up to 69% were observed by confocal microscopy during an actin cytoskeleton study. Such cytoskeletal changes and the proposed altered cell motility were confirmed by scratch-test (revealed a three-fold decrease in cell motility).<\/jats:p>","DOI":"10.3390\/ijms27146231","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T09:51:09Z","timestamp":1783936269000},"page":"6231","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Study of Cytotoxicity of Pyrrolo[3,4-d]isoxazoline and Pyrrolo[2,1-a]isoquinoline Derivatives Against Tumor Cell Lines"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2990-5958","authenticated-orcid":false,"given":"Andrew S.","family":"Drachuk","sequence":"first","affiliation":[{"name":"Laboratory of Nanobiotechnologies, Alferov University, ul. 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