{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T03:05:20Z","timestamp":1783998320166,"version":"3.55.0"},"reference-count":59,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T00:00:00Z","timestamp":1783900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Volatile organic compounds (VOCs) reflect cellular metabolic activities and may serve as non-invasive biomarkers in oncology. This study investigated whether acquired oxaliplatin resistance in colorectal cancer is associated with distinct volatilomic alterations. The human colorectal cancer cell line HCT116 and its oxaliplatin-resistant derivative (OXrHCT116) were analyzed under basal conditions and following oxaliplatin exposure. Chemoresistance was confirmed using dose\u2013response, cell viability, and colony formation assays. VOCs were analyzed by headspace needle trap extraction coupled with gas chromatography\u2013mass spectrometry (HS-NTE-GC-MS). OXrHCT116 cells exhibited markedly reduced oxaliplatin sensitivity, increased IC50 values, and reduced proliferative and clonogenic capacity. Volatilomic profiling identified 55 significantly altered VOCs. Parental HCT116 cells displayed broader VOC diversity and higher turnover than OXrHCT116 cells. Hydrocarbons associated with lipid peroxidation and oxidative stress were more abundant in HCT116 cells, whereas resistant cells showed markedly reduced emission of these compounds. Additional alterations in aldehydes, alcohols, and aromatic compounds suggested reduced metabolic flux in resistant cells. Oxaliplatin exposure induced pronounced volatilomic changes in HCT116 cells but only minimal modulation in OXrHCT116 cells. These findings suggest that oxaliplatin resistance may be associated with distinct metabolic reprogramming and support VOC profiling as a promising approach for monitoring chemoresistance.<\/jats:p>","DOI":"10.3390\/ijms27146240","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T02:34:44Z","timestamp":1783996484000},"page":"6240","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Volatilomic Signatures of Parental and Oxaliplatin-Resistant HCT116 Colon Cancer Cell Lines"],"prefix":"10.3390","volume":"27","author":[{"given":"Christine","family":"Heinzle","sequence":"first","affiliation":[{"name":"Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), 6800 Feldkirch, Austria"},{"name":"Central Medical Laboratories, 6800 Feldkirch, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3776-5704","authenticated-orcid":false,"given":"Andreas","family":"Leiherer","sequence":"additional","affiliation":[{"name":"Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), 6800 Feldkirch, Austria"},{"name":"Central Medical Laboratories, 6800 Feldkirch, Austria"},{"name":"Faculty of Medical Sciences, Private University in the Principality of Liechtenstein, FL-9495 Triesen, Liechtenstein"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5582-4125","authenticated-orcid":false,"given":"Axel","family":"Muendlein","sequence":"additional","affiliation":[{"name":"Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), 6800 Feldkirch, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3592-2059","authenticated-orcid":false,"given":"Clemens","family":"Ager","sequence":"additional","affiliation":[{"name":"Institute for Breath Research, University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4657-1832","authenticated-orcid":false,"given":"Agnieszka","family":"Kr\u00f3licka","sequence":"additional","affiliation":[{"name":"Department of Building Materials Technology, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris A.","family":"Mayhew","sequence":"additional","affiliation":[{"name":"Institute for Breath Research, University of Innsbruck, 6020 Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawel","family":"Mochalski","sequence":"additional","affiliation":[{"name":"Institute for Breath Research, University of Innsbruck, 6020 Innsbruck, Austria"},{"name":"Institute of Chemistry, Jan Kochanowski University, 25-369 Kielce, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1111\/apt.15140","article-title":"Volatile Organic Compounds Emitted from Faeces as a Biomarker for Colorectal Cancer","volume":"49","author":"Bond","year":"2019","journal-title":"Aliment. 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