{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:37:20Z","timestamp":1785429440341,"version":"3.56.0"},"reference-count":57,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo (ANID), Chile","award":["21240274"],"award-info":[{"award-number":["21240274"]}]},{"award":["21240274"],"award-info":[{"award-number":["21240274"]}],"id":[{"id":"https:\/\/ror.org\/02ap3w078","id-type":"ROR","asserted-by":"publisher"}]},{"name":"University of the Frontera"},{"id":[{"id":"https:\/\/ror.org\/04v0snf24","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Helicobacter pylori infection is associated with severe gastroduodenal pathologies. This study evaluated the bactericidal and antibiofilm activities of eudesmin, a furanofuran lignan from Araucaria araucana, and its acid-induced epimer, epieudesmin, against clinical isolates and the H. pylori ATCC 43504 strain. Virulence profiles and antibiotic susceptibility (CLSI\/EUCAST) were determined. Antibacterial effects were assessed via minimum inhibitory concentration (MIC), checkerboard assays, time-kill kinetics, and urease activity. Biofilm reduction and morphological alterations were analyzed using crystal violet staining and scanning electron microscopy, respectively. Cytotoxicity was evaluated in HepG2 cells. Clinical strains exhibited distinct virulence and multidrug-resistance patterns. Both lignans displayed rapid antibacterial activity (MIC: 0.78\u20133.12 \u00b5g\/mL) without antagonistic interactions with co-administered antibiotics and significantly reduced biofilm biomass (p &lt; 0.0001). Eudesmin and epieudesmin effectively inhibit H. pylori growth and biofilm formation, while sublethal concentrations disrupted biofilm integrity and induced a bacillary-to-coccoid transition. Toxicity of both compounds was evaluated in HepG2 cells till 50 \u00b5g\/mL showing a similar pattern, reducing cell viability up to 30% at almost 25 \u00b5g\/mL, highlighting its potential as a non-antibiotic complementary therapeutic agent, although further in vivo and clinical studies are warranted to confirm its therapeutic applicability.<\/jats:p>","DOI":"10.3390\/ijms27146295","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T06:49:36Z","timestamp":1784184576000},"page":"6295","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acid-Induced Epimerization of Eudesmin to Epieudesmin: Rapid Bactericidal and Antibiofilm Activity Against Helicobacter pylori"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9836-8107","authenticated-orcid":false,"given":"Cristian","family":"Parra-Sep\u00falveda","sequence":"first","affiliation":[{"name":"Laboratory of Bacterial Pathogenicity, Faculty of Biological Sciences, Universidad de Concepci\u00f3n, Concepcion 4030000, Chile"},{"name":"Doctorado en Ciencias Menci\u00f3n Biolog\u00eda Celular y Molecular Aplicada, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6451-6810","authenticated-orcid":false,"given":"Kimberly","family":"S\u00e1nchez-Alonzo","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Pathogenicity, Faculty of Biological Sciences, Universidad de Concepci\u00f3n, Concepcion 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Becerra","sequence":"additional","affiliation":[{"name":"Departamento de Bot\u00e1nica, Facultad de Ciencias Naturales y Oceanogr\u00e1ficas, Universidad de Concepcion, Concepcion 4070386, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8521-1469","authenticated-orcid":false,"given":"Leandro","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Qu\u00edmicas, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia 5090000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Burgos","sequence":"additional","affiliation":[{"name":"Escuela de Tecnolog\u00eda M\u00e9dica, Centro de Investigaci\u00f3n en Prevenci\u00f3n y Cuidados de la Salud (+SALUD), Facultad de Salud, Universidad Santo Tom\u00e1s, Temuco 4780000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9066-1763","authenticated-orcid":false,"given":"Cecilia","family":"Villegas","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias Qu\u00edmicas y Biol\u00f3gicas, Facultad de Recursos Naturales, Universidad Cat\u00f3lica de Temuco, Rudecindo Ortega, Temuco 4781509, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe A.","family":"Sanhueza","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products & Drug Discovery, Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjam\u00edn","family":"Oporto","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products & Drug Discovery, Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristobal","family":"Goic","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Pathogenicity, Faculty of Biological Sciences, Universidad de Concepci\u00f3n, Concepcion 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3909-9733","authenticated-orcid":false,"given":"Apolinaria","family":"Garc\u00eda-Cancino","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Pathogenicity, Faculty of Biological Sciences, Universidad de Concepci\u00f3n, Concepcion 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4668-4984","authenticated-orcid":false,"given":"Cristian","family":"Paz","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products & Drug Discovery, Department of Basic Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco 4811230, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e12537","DOI":"10.1111\/hel.12537","article-title":"Helicobacter pylori and non-alcoholic fatty liver disease: A new enigma?","volume":"23","author":"Mousa","year":"2018","journal-title":"Helicobacter"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.4103\/jms.jms_25_17","article-title":"World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics","volume":"32","author":"Shrivastava","year":"2018","journal-title":"JMS-J. 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