{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:34:21Z","timestamp":1785418461405,"version":"3.56.0"},"reference-count":85,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T00:00:00Z","timestamp":1784073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["RS-2026-25493296"],"award-info":[{"award-number":["RS-2026-25493296"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["RS-2023-00211685"],"award-info":[{"award-number":["RS-2023-00211685"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["RS-2024-00463065"],"award-info":[{"award-number":["RS-2024-00463065"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"award":["RS-2026-25493296"],"award-info":[{"award-number":["RS-2026-25493296"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"publisher"}]},{"award":["RS-2023-00211685"],"award-info":[{"award-number":["RS-2023-00211685"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"publisher"}]},{"award":["RS-2024-00463065"],"award-info":[{"award-number":["RS-2024-00463065"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Ministry of Health and Welfare","award":["RS-2026-25536079"],"award-info":[{"award-number":["RS-2026-25536079"]}]},{"award":["RS-2026-25536079"],"award-info":[{"award-number":["RS-2026-25536079"]}],"id":[{"id":"https:\/\/ror.org\/00vxgjw72","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Extracellular vesicles (EVs), particularly small EVs or exosomes, are promising cell-free therapeutics with superior biocompatibility and intrinsic targeting for synthetic nanoparticles. However, conventional bulk preparation methods suffer from low yield, poor reproducibility, and structural instability. Microfluidic technologies resolve these issues by enabling precise, automated, and low-shear fluidic manipulation. This mini-review highlights recent advances in microfluidic-engineered exosomes for cancer immunotherapy and infectious diseases. We evaluate critical microfluidic strategies for isolation, surface engineering, and cargo loading, contrasting platforms like ExoArc, acoustofluidics, cellular nanoporation, and electroporation. Particular emphasis is placed on complex modalities, including immune cell-derived exosomes (IEX), neo-antigen presentation, chimeric antigen receptor (CAR)-derived exosomes, and targeted siRNA delivery networks. Crucially, we analyze the technological disconnect between analytical microfluidic scales and massive therapeutic manufacturing volumes, addressing how physical forces risk damaging conformationally sensitive surface proteins (e.g., CAR scFv). Finally, we outline future perspectives, including high-throughput 3D-multiplexed networks, stimulus-responsive scarless elution, and integrated \u201csample-to-therapy\u201d circuits. Guided by the MISEV2023 guidelines, this review frames the path toward standardized, clinical-scale engineering of multi-functional, cell-free immunotherapies.<\/jats:p>","DOI":"10.3390\/ijms27146298","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T06:49:36Z","timestamp":1784184576000},"page":"6298","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microfluidic Platforms for Exosome Engineering: Scalable Therapeutics for Cancer Immunotherapy and Infectious Diseases"],"prefix":"10.3390","volume":"27","author":[{"given":"Minyoung","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Biomedical Technology, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4022-3415","authenticated-orcid":false,"given":"Kwangmin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jungho","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1923-9997","authenticated-orcid":false,"given":"Kyung-A","family":"Hyun","sequence":"additional","affiliation":[{"name":"School of Biopharmaceutical and Medical Sciences, Sungshin Women\u2019s University, 55 Dobong-ro 76 ga-gil, Gangbuk-gu, Seoul 01133, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8142-0063","authenticated-orcid":false,"given":"Anbazhagan","family":"Sathiyaseelan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Technology, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunyoung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Technology, Kangwon National University, Chuncheon 24341, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"iyae088","DOI":"10.1093\/genetics\/iyae088","article-title":"Extracellular vesicles","volume":"227","author":"Wang","year":"2024","journal-title":"Genetics"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41392-024-01735-1","article-title":"Extracellular vesicles as tools and targets in therapy for diseases","volume":"9","author":"Kumar","year":"2024","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1038\/s41401-022-00902-w","article-title":"Current advances in the use of exosomes, liposomes, and bioengineered hybrid nanovesicles in cancer detection and therapy","volume":"43","author":"Mukherjee","year":"2022","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"eaau6977","DOI":"10.1126\/science.aau6977","article-title":"The biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1080\/10717544.2020.1748758","article-title":"Prospects and challenges of extracellular vesicle-based drug delivery system: Considering cell source","volume":"27","author":"Meng","year":"2020","journal-title":"Drug Deliv."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.biochi.2022.09.016","article-title":"Exosomal lipids from membrane organization to biomarkers: Focus on an endolysosomal-specific lipid","volume":"203","author":"Colosetti","year":"2022","journal-title":"Biochimie"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2400623","DOI":"10.1002\/adbi.202400623","article-title":"The Potential for Extracellular Vesicles in Nanomedicine: A Review of Recent Advancements and Challenges Ahead","volume":"9","author":"Ebrahimi","year":"2025","journal-title":"Adv. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2005709","DOI":"10.1002\/adma.202005709","article-title":"Engineered extracellular vesicles for cancer therapy","volume":"33","author":"Zhang","year":"2021","journal-title":"Adv. Mater."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6623","DOI":"10.1021\/acsnano.3c12862","article-title":"High-Throughput Microfluidic Extraction of Platelet-free Plasma for MicroRNA and Extracellular Vesicle Analysis","volume":"18","author":"Leong","year":"2024","journal-title":"ACS Nano"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s12672-026-04748-0","article-title":"Exosomes serve as natural nanocarriers targeting cancer stem cells to advance precision oncology","volume":"17","author":"AlRamadneh","year":"2026","journal-title":"Discov. Oncol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Jin, Y., Zhang, Y., Song, M., Zhou, J., Zhang, H., and Ding, Y. (2026). Engineered exosomes as precision tools for brain-targeted drug delivery and treatment of central nervous system diseases. Cell Biomater., 100344.","DOI":"10.1016\/j.celbio.2025.100344"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Onohuean, H., Naik Bukke, S.P., Thalluri, C., Abass, K.S., and Choonara, Y.E. (2025). Exosome engineering for targeted therapy of brain-infecting pathogens: Molecular tools, delivery platforms, and translational advances. Front. Med. Technol., 7.","DOI":"10.3389\/fmedt.2025.1655471"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2402222","DOI":"10.1002\/smtd.202402222","article-title":"Exosome-Based Vaccines: Pioneering New Frontiers in Combating Infectious Diseases and Cancer","volume":"9","author":"Wang","year":"2025","journal-title":"Small Methods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1038\/s41392-023-01382-y","article-title":"Engineered exosomes from different sources for cancer-targeted therapy","volume":"8","author":"Zhang","year":"2023","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"101066","DOI":"10.1016\/j.nantod.2020.101066","article-title":"Towards Microfluidic-Based Exosome Isolation and Detection for Tumor Therapy","volume":"37","author":"Wang","year":"2021","journal-title":"Nano Today"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3558","DOI":"10.1039\/C7LC00592J","article-title":"Microfluidics for exosome isolation and analysis: Enabling liquid biopsy for personalized medicine","volume":"17","author":"Wu","year":"2017","journal-title":"Lab Chip"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ubanako, P., Mirza, S., Ruff, P., and Penny, C. (2024). Exosome-mediated delivery of siRNA molecules in cancer therapy: Triumphs and challenges. Front. Mol. Biosci., 11.","DOI":"10.3389\/fmolb.2024.1447953"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e70132","DOI":"10.1002\/jev2.70132","article-title":"Microfluidic Devices for Manufacture of Therapeutic Extracellular Vesicles: Advances and Opportunities","volume":"14","author":"Sango","year":"2025","journal-title":"J. Extracell. Vesicles"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"9443","DOI":"10.1021\/jacs.2c02268","article-title":"Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies","volume":"144","author":"Sheng","year":"2022","journal-title":"J. Am. Chem. Soc."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/s41378-026-01273-6","article-title":"Parallelized droplet microfluidic mechanoporation enables robust and clogging-resistant intracellular gene delivery","volume":"12","author":"Liu","year":"2026","journal-title":"Microsyst. Nanoeng."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2321","DOI":"10.1021\/nn506538f","article-title":"Acoustic Purification of Extracellular Microvesicles","volume":"9","author":"Lee","year":"2015","journal-title":"ACS Nano"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/s41378-022-00374-2","article-title":"Acoustofluidics for simultaneous nanoparticle-based drug loading and exosome encapsulation","volume":"8","author":"Wang","year":"2022","journal-title":"Microsyst. Nanoeng."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/s41392-025-02312-w","article-title":"Extracellular vesicle-based drug overview: Research landscape, quality control and nonclinical evaluation strategies","volume":"10","author":"Xu","year":"2025","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1186\/s12951-024-02634-x","article-title":"Exosome for mRNA delivery: Strategies and therapeutic applications","volume":"22","author":"Iqbal","year":"2024","journal-title":"J. Nanobiotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/s41551-019-0485-1","article-title":"Large-scale generation of functional mRNA-encapsulating exosomes via cellular nanoporation","volume":"4","author":"Yang","year":"2020","journal-title":"Nat. Biomed. Eng."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2191","DOI":"10.1021\/acs.chemmater.9b00050","article-title":"Surface Engineering of Extracellular Vesicles through Chemical and Biological Strategies","volume":"31","author":"Richardson","year":"2019","journal-title":"Chem. Mater."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1186\/s12964-025-02268-y","article-title":"Functionalized exosomes for targeted therapy in cancer and regenerative medicine: Genetic, chemical, and physical modifications","volume":"23","author":"Gabaran","year":"2025","journal-title":"Cell Commun. Signal."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s10787-022-01115-7","article-title":"Exosome engineering in cell therapy and drug delivery","volume":"31","author":"Sadeghi","year":"2023","journal-title":"Inflammopharmacology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1186\/s12951-026-04404-3","article-title":"Surface engineering of extracellular vesicles: Trends, strategies, and applications in diagnosis and therapy","volume":"24","author":"Hong","year":"2026","journal-title":"J. Nanobiotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e70215","DOI":"10.1002\/jev2.70215","article-title":"Universal Microfluidic Platform for Multifunctional Surface Modification of Small Extracellular Vesicles","volume":"14","author":"Hong","year":"2025","journal-title":"J. Extracell. Vesicles"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1039\/C8LC01279B","article-title":"Microfluidic on-demand engineering of exosomes towards cancer immunotherapy","volume":"19","author":"Zhao","year":"2019","journal-title":"Lab Chip"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"115810","DOI":"10.1016\/j.addr.2026.115810","article-title":"Microfluidics for separation, detection, and engineering of extracellular vesicles","volume":"231","author":"Heikal","year":"2026","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1021\/acs.bioconjchem.2c00019","article-title":"Suppressing or Enhancing Macrophage Engulfment through the Use of CD47 and Related Peptides","volume":"33","author":"Jalil","year":"2022","journal-title":"Bioconjug Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature22341","article-title":"Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer","volume":"546","author":"Kamerkar","year":"2017","journal-title":"Nature"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"123510","DOI":"10.1016\/j.biomaterials.2025.123510","article-title":"Engineered exosomes for targeted microRNA delivery to reverse liver fibrosis","volume":"324","author":"Zhang","year":"2026","journal-title":"Biomaterials"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"106929","DOI":"10.1016\/j.ejps.2024.106929","article-title":"Microfluidic post-insertion of polyethylene glycol lipids and KK or RGD high functionality and quality lipids in milk-derived extracellular vesicles","volume":"203","author":"Geng","year":"2024","journal-title":"Eur. J. Pharm. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"10712","DOI":"10.1021\/acsnano.7b04878","article-title":"The Exosome Total Isolation Chip","volume":"11","author":"Liu","year":"2017","journal-title":"ACS Nano"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"6610","DOI":"10.1038\/s41467-023-42365-5","article-title":"Adaptive design of mRNA-loaded extracellular vesicles for targeted immunotherapy of cancer","volume":"14","author":"Dong","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Han, Y., Jones, T.W., Dutta, S., Zhu, Y., Wang, X., Narayanan, S.P., Fagan, S.C., and Zhang, D. (2021). Overview and Update on Methods for Cargo Loading into Extracellular Vesicles. Processes, 9.","DOI":"10.3390\/pr9020356"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Romagnoli, G.G., Zelante, B.B., Toniolo, P.A., Migliori, I.K., and Barbuto, J.A.M. (2015). Dendritic cell-derived exosomes may be a tool for cancer immunotherapy by converting tumor cells into immunogenic targets. Front. Immunol., 5.","DOI":"10.3389\/fimmu.2014.00692"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s12276-023-01132-8","article-title":"Modification of immune cell-derived exosomes for enhanced cancer immunotherapy: Current advances and therapeutic applications","volume":"56","author":"Jung","year":"2024","journal-title":"Exp. Mol. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1186\/s13287-024-03783-4","article-title":"CAR-T cell-derived exosomes: A new perspective for cancer therapy","volume":"15","author":"Sani","year":"2024","journal-title":"Stem Cell Res. Ther."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/s41392-025-02269-w","article-title":"CAR-T cell therapy for cancer: Current challenges and future directions","volume":"10","author":"Zugasti","year":"2025","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1655095","DOI":"10.3389\/fimmu.2025.1655095","article-title":"CAR-exosomes derived from immune cells: An emerging nanoscale vanguard in overcoming tumor immunotherapy hurdles","volume":"16","author":"Zhao","year":"2025","journal-title":"Front Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1186\/s41232-024-00358-x","article-title":"Focusing on exosomes to overcome the existing bottlenecks of CAR-T cell therapy","volume":"44","author":"Zhang","year":"2024","journal-title":"Inflamm. Regen."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4355","DOI":"10.1038\/s41467-019-12321-3","article-title":"CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity","volume":"10","author":"Fu","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e99263","DOI":"10.1172\/jci.insight.99263","article-title":"Generation and testing of clinical-grade exosomes for pancreatic cancer","volume":"3","author":"Mendt","year":"2018","journal-title":"JCI Insight"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"8696","DOI":"10.1038\/s41467-025-63718-2","article-title":"Engineered exosomes with KrasG12D specific siRNA in pancreatic cancer: A phase I study with immunological correlates","volume":"16","author":"Kalluri","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e12508","DOI":"10.1002\/jev2.12508","article-title":"Scalable production of siRNA-encapsulated extracellular vesicles for the inhibition of KRAS-mutant cancer using acoustic shock waves","volume":"13","author":"Kim","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Yuan, R., Mu, Z., Zhang, H., Guo, J., Tian, Y., Xin, Q., Zhu, X., Dong, Z., Wang, H., and Shi, Y. (2025). Ultrasonic Microfluidic Method Used for siHSP47 Loaded in Human Embryonic Kidney Cell-Derived Exosomes for Inhibiting TGF-\u03b21 Induced Fibroblast Differentiation and Migration. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26010382"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3185","DOI":"10.1039\/D5LC00279F","article-title":"An integrated microfluidic chip for synchronous drug loading, separation and detection of plasma exosomes","volume":"25","author":"Zhang","year":"2025","journal-title":"Lab Chip"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"140022","DOI":"10.1016\/j.snb.2026.140022","article-title":"An integrated microfluidic platform for exosome efficient separation and drug loading","volume":"462","author":"Tong","year":"2026","journal-title":"Sens. Actuators B Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/s41551-022-00902-5","article-title":"Exosomes decorated with a recombinant SARS-CoV-2 receptor-binding domain as an inhalable COVID-19 vaccine","volume":"6","author":"Wang","year":"2022","journal-title":"Nat. Biomed. Eng."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Raghav, A., Khan, Z.A., Upadhayay, V.K., Tripathi, P., Gautam, K.A., Mishra, B.K., Ahmad, J., and Jeong, G.B. (2021). Mesenchymal Stem Cell-Derived Exosomes Exhibit Promising Potential for Treating SARS-CoV-2-Infected Patients. Cells, 10.","DOI":"10.3390\/cells10030587"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1186\/s13287-026-04980-z","article-title":"Mesenchymal stem cell derived exosomes mitigate COVID-19 cytokine storm via Annexin A1 and TGF-\u03b2 mediated MAPK pathway inhibition","volume":"17","author":"Ebrahim","year":"2026","journal-title":"Stem Cell Res. Ther."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e2500139","DOI":"10.1002\/advs.202500139","article-title":"Locoregional Immune Checkpoint Blockade and Remodeling of Lymph Nodes by Engineered Dendritic Cell-Derived Exosomes for Suppressing Tumor Progression and Metastasis","volume":"12","author":"Wang","year":"2025","journal-title":"Adv. Sci."},{"key":"ref_57","first-page":"466","article-title":"Gene engineered exosome reverses T cell exhaustion in cancer immunotherapy","volume":"34","author":"Li","year":"2024","journal-title":"Bioact. Mater."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Huang, C., Hu, Q., Wang, P., Xie, M., Zhang, Y., Li, Z., Tang, S., Zhang, Y., Tian, Z., and Liu, X. (2025). Overexpression of NKG2D and IL24 in NK Cell-Derived Exosomes for Cancer Therapy. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26052098"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4310","DOI":"10.1038\/s41467-026-70924-z","article-title":"Spatiotemporally engineered tumor-derived extracellular vesicle-based scaffold vaccine for personalized cancer immunotherapy","volume":"17","author":"Chen","year":"2026","journal-title":"Nat. Commun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"108708","DOI":"10.1016\/j.isci.2023.108708","article-title":"SARS-CoV-2 antigen-carrying extracellular vesicles activate T cell responses in a human immunogenicity model","volume":"27","author":"Cummings","year":"2024","journal-title":"iScience"},{"key":"ref_61","first-page":"573","article-title":"Fc-empowered exosomes with superior epithelial layer transmission and lung distribution ability for pulmonary vaccination","volume":"42","author":"Meng","year":"2024","journal-title":"Bioact. Mater."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Cacciottolo, M., Hsieh, L.-E., Li, Y., LeClaire, M.J., Mora, C.L., Lau, C., Dwyer, C., Elliott, K., and Sun, M. (2025). Multivalent Exosome based protein vaccine: A \u201cmix and match\u201d approach to epidemic viruses\u2019 challenges. Vaccines, 13.","DOI":"10.3390\/vaccines13030258"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Shen, A., Zhu, S., Li, M., Zhao, Y., Wu, Y., Zhang, Y., Zhang, J., Han, X., Wang, Y., and Zhao, G. (2026). Synergistic immune protection of exosomal T-cell epitope vaccine and antibody-inducing vaccine against SARS-CoV-2 in highly humanized mice. Front. Immunol., 17.","DOI":"10.3389\/fimmu.2026.1729444"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"26649","DOI":"10.1038\/s41598-024-77258-0","article-title":"Mesenchymal stem cell-derived extracellular vesicles reduce inflammatory responses to SARS-CoV-2 and Influenza viral proteins via miR-146a\/NF-\u03baB pathway","volume":"14","author":"Anggraeni","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1186\/s13287-025-04576-z","article-title":"Inflammatory cytokine-primed MSC-derived extracellular vesicles ameliorate acute lung injury via enhanced immunomodulation and alveolar repair","volume":"16","author":"Jeong","year":"2025","journal-title":"Stem Cell Res. Ther."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/s10020-024-00959-9","article-title":"Probiotic bacteria-released extracellular vesicles enhance macrophage phagocytosis in polymicrobial sepsis by activating the FPR1\/2 pathway","volume":"30","author":"Zhu","year":"2024","journal-title":"Mol. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e70000","DOI":"10.1002\/jex2.70000","article-title":"Engineered extracellular vesicles coated with an antimicrobial peptide for advanced control of bacterial sepsis","volume":"3","author":"Ibrahim","year":"2024","journal-title":"J. Extracell. Biol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"45724","DOI":"10.1038\/s41598-025-28377-9","article-title":"Cathelicidin LL37-loaded extracellular vesicles from Edwardsiella piscicida promote antibacterial and wound-healing activity","volume":"15","author":"Dias","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"113542","DOI":"10.1016\/j.isci.2025.113542","article-title":"Extracellular vesicles: Antiviral functions and applications in therapeutics and vaccines","volume":"28","author":"Gao","year":"2025","journal-title":"iScience"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1038\/s41565-021-00931-2","article-title":"Extracellular vesicles as a next-generation drug delivery platform","volume":"16","author":"Herrmann","year":"2021","journal-title":"Nat. Nanotechnol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"e12404","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Palviainen, M., Saraswat, M., Varga, Z., Kitka, D., Neuvonen, M., Puhka, M., Joenv\u00e4\u00e4r\u00e4, S., Renkonen, R., Nieuwland, R., and Takatalo, M. (2020). Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo-Implications for biomarker discovery. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0236439"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"8142","DOI":"10.1021\/acsnano.5b03300","article-title":"In Vivo Biomolecule Corona around Blood-Circulating, Clinically Used and Antibody-Targeted Lipid Bilayer Nanoscale Vesicles","volume":"9","author":"Hadjidemetriou","year":"2015","journal-title":"ACS Nano"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/nbt.1807","article-title":"Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes","volume":"29","author":"Seow","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1016\/j.cell.2014.09.051","article-title":"Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways","volume":"159","author":"Boelens","year":"2014","journal-title":"Cell"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.cell.2017.06.031","article-title":"Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer","volume":"170","author":"Nabet","year":"2017","journal-title":"Cell"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Gangadaran, P., Hong, C.M., Oh, J.M., Rajendran, R.L., Kalimuthu, S., Son, S.H., Gopal, A., Zhu, L., Baek, S.H., and Jeong, S.Y. (2018). In vivo Non-invasive Imaging of Radio-Labeled Exosome-Mimetics Derived from Red Blood Cells in Mice. Front. Pharmacol., 9.","DOI":"10.3389\/fphar.2018.00817"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"10883","DOI":"10.1021\/acsnano.7b04495","article-title":"In Vivo Neuroimaging of Exosomes Using Gold Nanoparticles","volume":"11","author":"Betzer","year":"2017","journal-title":"ACS Nano"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Al Shammrie, F.F., Alhemshy, L.Z., Althawy, M.M., Alfaraj, M.M., Alotaibi, A.S., Alali, D.S., Alsaggaf, O.H., Alhamashi, L.Z., and Albelowi, L.M. (2025). The Efficacy of MSC-Derived Exosome-Based Therapies in Treating Scars, Aging and Hyperpigmentation: A Systematic Review of Human Clinical Outcomes. Reports, 8.","DOI":"10.3390\/reports8040268"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Gimona, M., Pachler, K., Laner-Plamberger, S., Schallmoser, K., and Rohde, E. (2017). Manufacturing of Human Extracellular Vesicle-Based Therapeutics for Clinical Use. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18061190"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"eaav8521","DOI":"10.1126\/scitranslmed.aav8521","article-title":"Advances in therapeutic applications of extracellular vesicles","volume":"11","author":"Wiklander","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1016\/j.jconrel.2022.12.025","article-title":"The therapeutic and commercial landscape of stem cell vesicles in regenerative dermatology","volume":"353","author":"Davies","year":"2023","journal-title":"J. Control. Release"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"959","DOI":"10.32604\/biocell.2023.028371","article-title":"Microfluidic methods used in exosome isolation","volume":"47","author":"Ozcelik","year":"2023","journal-title":"Biocell"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"e12458","DOI":"10.1002\/jev2.12458","article-title":"A systematic review and meta-analysis of clinical trials assessing safety and efficacy of human extracellular vesicle-based therapy","volume":"13","author":"Derdelinckx","year":"2024","journal-title":"J. Extracell. Vesicles"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"711565","DOI":"10.3389\/fimmu.2021.711565","article-title":"Exosome-Based Vaccines: History, Current State, and Clinical Trials","volume":"12","author":"Santos","year":"2021","journal-title":"Front Immunol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/14\/6298\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:15:22Z","timestamp":1784261722000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/14\/6298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,15]]},"references-count":85,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ijms27146298"],"URL":"https:\/\/doi.org\/10.3390\/ijms27146298","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,15]]}}}