{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:15:30Z","timestamp":1785413730234,"version":"3.56.0"},"reference-count":78,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82300674"],"award-info":[{"award-number":["82300674"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["82300674"],"award-info":[{"award-number":["82300674"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Liaoning Normal University","award":["2024BSL005"],"award-info":[{"award-number":["2024BSL005"]}]},{"award":["2024BSL005"],"award-info":[{"award-number":["2024BSL005"]}],"id":[{"id":"https:\/\/ror.org\/04c3cgg32","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Inherited liver diseases are predominantly caused by monogenic mutations, and the vast majority of these conditions currently lack curative treatment options. Although liver transplantation may be used for patients with end-stage disease, it faces numerous challenges, including donor organ shortage, immune rejection, and the need for lifelong immunosuppression. In recent years, CRISPR-Cas9-based gene editing technology has advanced rapidly, offering transformative hope for the treatment of these diseases. This review systematically elucidates the working principles and technical advantages of the CRISPR-Cas9 system and its derived tools (base editing and prime editing), summarizes recent applications of these technologies in the treatment of hereditary liver diseases, and discusses the prospects and challenges of their clinical translation, aiming to provide a theoretical reference for future research in this field.<\/jats:p>","DOI":"10.3390\/ijms27146469","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T10:35:52Z","timestamp":1784630152000},"page":"6469","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of CRISPR-Cas9-Based Gene Editing Technology in Inherited Liver Diseases"],"prefix":"10.3390","volume":"27","author":[{"given":"Ran","family":"Liu","sequence":"first","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Biotechnology and Molecular Drug Research and Development, School of Life Sciences, Liaoning Normal University, Dalian 116082, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqi","family":"Cong","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Biotechnology and Molecular Drug Research and Development, School of Life Sciences, Liaoning Normal University, Dalian 116082, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Biotechnology and Molecular Drug Research and Development, School of Life Sciences, Liaoning Normal University, Dalian 116082, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Xu","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Biotechnology and Molecular Drug Research and Development, School of Life Sciences, Liaoning Normal University, Dalian 116082, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1753-241X","authenticated-orcid":false,"given":"Xiaoxia","family":"Shi","sequence":"additional","affiliation":[{"name":"Liaoning Provincial Key Laboratory of Biotechnology and Molecular Drug Research and Development, School of Life Sciences, Liaoning Normal University, Dalian 116082, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Chen, Y., van Til, N.P., and Bosma, P.J. 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