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J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Gut microbiota dysbiosis and metabolic disorders are key factors in inflammatory Bowel Disease (IBD) pathogenesis and progression. Although Artemisia rupestris water extract (AR) contains many anti-inflammatory compounds, its effects and mechanisms in colitis have not yet been explored. This study is the first to evaluate AR\u2019s therapeutic efficacy in dextran sodium sulfate (DSS)-induced colitis and to investigate whether AR acts by modifying the gut microbiota and correcting metabolic imbalances. An acute inflammatory bowel disease model was established in mice using 3% DSS. Mice received AR at different doses, and clinical and pathological indices, histopathological scores, and blood concentrations of pro-inflammatory cytokines were evaluated. We explored AR; mechanisms in IBD using 16S rRNA sequencing and untargeted metabolomics. AR decreased concentrations of pro-inflammatory cytokines IL-6, CXCL-1, and TNF-\u03b1, and reduced MPO levels. It reduced intestinal permeability and mitigated IBD symptoms. Immunohistochemistry demonstrated that AR downregulated TLR4 and TLR9, while restoring Occludin (OCC) and Zonula Occludens-1 (ZO-1). AR modified the gut microbiota by reducing aberrant taxa such as HT002 and Erysipelatoclostridium, while increasing beneficial bacteria including Bacteroides, Alloprevotella, and Lachnospiraceae_NK4A136_group. AR also rectified metabolic disturbances by reducing 5OH-HIP, reversing decreases in metabolites such as 4-(2-amino-3-hydroxyphenyl)-2,4-dioxobutanoate and vanillin, and influencing amino acid and lipid metabolic pathways. AR treatment produces therapeutic effects distinct from other interventions by targeting inflammatory responses, restoring the gut microbiota and metabolism, suppressing TLR4 and TLR9 signaling, and repairing the mucosal barrier.<\/jats:p>","DOI":"10.3390\/ijms27146478","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:17:52Z","timestamp":1784647072000},"page":"6478","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Artemisia rupestris Improves the Microbiota\u2013Barrier Axis in DSS Colitis"],"prefix":"10.3390","volume":"27","author":[{"given":"Jiaying","family":"Wu","sequence":"first","affiliation":[{"name":"College of Pharmacy, Xinjiang Medical University, Urumqi 830017, China"},{"name":"Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Xinjiang Medical University, Urumqi 830017, China"},{"name":"Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Xinjiang Medical University, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuwen","family":"Mao","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Xinjiang Medical University, Urumqi 830017, China"},{"name":"Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Xinjiang Medical University, Urumqi 830017, China"},{"name":"Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Xinjiang Medical University, Urumqi 830017, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.pop.2017.07.010","article-title":"An Update on Inflammatory Bowel Disease","volume":"44","author":"Sairenji","year":"2017","journal-title":"Prim. Care"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1186\/s40168-022-01353-5","article-title":"Selected commensals educate the intestinal vascular and immune system for immunocompetence","volume":"10","author":"Romero","year":"2022","journal-title":"Microbiome"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/s41575-020-00360-x","article-title":"The four epidemiological stages in the global evolution of inflammatory bowel disease","volume":"18","author":"Kaplan","year":"2021","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/j.dld.2017.01.141","article-title":"Use of corticosteroids and immunosuppressive drugs in inflammatory bowel disease: Clinical practice guidelines of the Italian Group for the Study of Inflammatory Bowel Disease","volume":"49","author":"Biancone","year":"2017","journal-title":"Dig. Liver Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"105358","DOI":"10.1016\/j.phrs.2020.105358","article-title":"Berberine improves colitis by triggering AhR activation by microbial tryptophan catabolites","volume":"164","author":"Jing","year":"2021","journal-title":"Pharmacol. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"105767","DOI":"10.1016\/j.phrs.2021.105767","article-title":"Dihydromyricetin improves DSS-induced colitis in mice via modulation of fecal-bacteria-related bile acid metabolism","volume":"171","author":"Dong","year":"2021","journal-title":"Pharmacol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"115821","DOI":"10.3748\/wjg.v32.i10.115821","article-title":"Mechanisms and therapeutic potential of traditional Chinese medicine for inflammatory bowel disease","volume":"32","author":"Zeng","year":"2026","journal-title":"World J. Gastroenterol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Nurlybekova, A., Kudaibergen, A., Kazymbetova, A., Amangeldi, M., Baiseitova, A., Ospanov, M., Aisa, H.A., Ye, Y., Ibrahim, M.A., and Jenis, J. (2022). Traditional Use, Phytochemical Profiles and Pharmacological Properties of Artemisia Genus from Central Asia. Molecules, 27.","DOI":"10.3390\/molecules27165128"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"121028","DOI":"10.1016\/j.jep.2025.121028","article-title":"Dual targeting by Artemisia rupestris L. disrupting TLR4\/NF-\u03baB signaling and macrophage driven immune evasion in hepatocellular carcinoma","volume":"358","author":"Zhang","year":"2026","journal-title":"J. Ethnopharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1002\/rcm.5289","article-title":"Characterization and identification of chemical compositions in the extract of Artemisia rupestris L. by liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry","volume":"26","author":"Gu","year":"2012","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"119525","DOI":"10.1016\/j.carbpol.2022.119525","article-title":"Immunoregulatory properties of the cultivated Artemisia rupestris L. polysaccharide as a potential adjuvant","volume":"291","author":"Li","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_12","first-page":"310","article-title":"Advances in studies on the rupestonic acid derivatives as anti-influenza agents","volume":"13","author":"Yong","year":"2013","journal-title":"Mini Rev. Med. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"107531","DOI":"10.1016\/j.toxicon.2023.107531","article-title":"Integrating network pharmacology and experimental validation to explore the mechanisms of luteolin in alleviating fumonisin B1-induced intestinal inflammatory injury","volume":"237","author":"Wen","year":"2024","journal-title":"Toxicon"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Li, Y., Yao, J., Han, C., Yang, J., Chaudhry, M.T., Wang, S., Liu, H., and Yin, Y. (2016). Quercetin, Inflammation and Immunity. Nutrients, 8.","DOI":"10.3390\/nu8030167"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.rvsc.2019.03.016","article-title":"Adjuvanticity of aqueous extracts of Artemisia rupestris L. for inactivated foot-and-mouth disease vaccine in mice","volume":"124","author":"Wang","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e0004523","DOI":"10.1128\/cmr.00045-23","article-title":"Role of the intestinal microbiota in contributing to weight disorders and associated comorbidities","volume":"37","author":"Neyrinck","year":"2024","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chen, Y., Cui, W., Li, X., and Yang, H. (2021). Interaction Between Commensal Bacteria, Immune Response and the Intestinal Barrier in Inflammatory Bowel Disease. Front. Immunol., 12.","DOI":"10.3389\/fimmu.2021.761981"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1924","DOI":"10.1053\/j.gastro.2021.08.047","article-title":"The tight junction protein ZO-1 is dispensable for barrier function but critical for effective mucosal repair","volume":"161","author":"Kuo","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1053\/j.gastro.2020.09.056","article-title":"Inflammatory Bowel Diseases (IBD) and the Microbiome-Searching the Crime Scene for Clues","volume":"160","author":"Lee","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10121","DOI":"10.1039\/D1FO02001C","article-title":"Jellyfish skin polysaccharides enhance intestinal barrier function and modulate the gut microbiota in mice with DSS-induced colitis","volume":"12","author":"Cao","year":"2021","journal-title":"Food Funct."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Aldars-Garc\u00eda, L., Chaparro, M., and Gisbert, J.P. (2021). Systematic Review: The Gut Microbiome and Its Potential Clinical Application in Inflammatory Bowel Disease. Microorganisms, 9.","DOI":"10.3390\/microorganisms9050977"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/19490976.2021.1900996","article-title":"Gut microbiota shape the inflammatory response in mice with an epithelial defect","volume":"13","author":"Wang","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1002\/jobm.201500726","article-title":"Acute dextran sulfate sodium (DSS)-induced colitis promotes gut microbial dysbiosis in mice","volume":"56","author":"Munyaka","year":"2016","journal-title":"J. Basic Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"15.25.1","DOI":"10.1002\/0471142735.im1525s104","article-title":"Dextran sulfate sodium (DSS)-induced colitis in mice","volume":"104","author":"Chassaing","year":"2014","journal-title":"Curr. Protoc. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1007\/s11427-017-9097-0","article-title":"Initial gut microbiota structure affects sensitivity to DSS-induced colitis in a mouse model","volume":"61","author":"Li","year":"2018","journal-title":"Sci. China Life Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Santana, P.T., Rosas, S.L.B., Ribeiro, B.E., Marinho, Y., and de Souza, H.S.P. (2022). Dysbiosis in inflammatory bowel disease: Pathogenic role and potential therapeutic targets. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23073464"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Spiljar, M., Merkler, D., and Trajkovski, M. (2017). The Immune System Bridges the Gut Microbiota with Systemic Energy Homeostasis: Focus on TLRs, Mucosal Barrier, and SCFAs. Front. Immunol., 8.","DOI":"10.3389\/fimmu.2017.01353"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1093\/ibd\/izad312","article-title":"Unveiling Colitis: A Journey through the Dextran Sodium Sulfate-induced Model","volume":"30","author":"Yang","year":"2024","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Iyer, N., Williams, M.A., O\u2019Callaghan, A.A., Dempsey, E., Cabrera-Rubio, R., Raverdeau, M., Crispie, F., Cotter, P.D., and Corr, S.C. (2022). Lactobacillus salivarius UCC118\u2122 dampens inflammation and promotes microbiota recovery to provide therapeutic benefit in a DSS-induced colitis model. Microorganisms, 10.","DOI":"10.3390\/microorganisms10071383"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s00253-021-11694-0","article-title":"Ligilactobacillus salivarius functionalities, applications, and manufacturing challenges","volume":"106","author":"Passot","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/mi.2016.75","article-title":"Mechanisms of inflammation-driven bacterial dysbiosis in the gut","volume":"10","author":"Zeng","year":"2017","journal-title":"Mucosal Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1073\/pnas.2316579120","article-title":"Gut dysbiosis: Ecological causes and causative effects on human disease","volume":"120","author":"Winter","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2361493","DOI":"10.1080\/19490976.2024.2361493","article-title":"Disruption of intestinal oxygen balance in acute colitis alters the gut microbiome","volume":"16","author":"Zong","year":"2024","journal-title":"Gut Microbes"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2431643","DOI":"10.1080\/19490976.2024.2431643","article-title":"Probiotic significance of Lactobacillus strains: A comprehensive review on health impacts, research gaps, and future prospects","volume":"16","author":"Shah","year":"2024","journal-title":"Gut Microbes"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4306","DOI":"10.1007\/s10620-023-08122-w","article-title":"Intestinal barrier dysfunction in inflammatory bowel disease: Underpinning pathogenesis and therapeutics","volume":"68","author":"Dunleavy","year":"2023","journal-title":"Dig. Dis. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7135","DOI":"10.1038\/s41467-023-42788-0","article-title":"Microbiome and metabolome features in inflammatory bowel disease via multi-omics integration analyses across cohorts","volume":"14","author":"Ning","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1073\/pnas.2501030122","article-title":"Human milk IgA promotes normal immune development by limiting Th17-inducing Erysipelatoclostridium ramosum in the infant gut","volume":"122","author":"Donald","year":"2025","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"G27","DOI":"10.1152\/ajpgi.00465.2012","article-title":"Paeoniflorin abrogates DSS-induced colitis via a TLR4-dependent pathway","volume":"306","author":"Zhang","year":"2014","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Mu\u00f1oz, F., Fonseca-Camarillo, G., Villeda-Ram\u00edrez, M.A., Miranda-P\u00e9rez, E., Mendivil, E.J., Barreto-Z\u00fa\u00f1iga, R., Uribe, M., Bojalil, R., Dom\u00ednguez-L\u00f3pez, A., and Yamamoto-Furusho, J.K. (2011). Transcript levels of Toll-Like Receptors 5, 8 and 9 correlate with inflammatory activity in Ulcerative Colitis. BMC Gastroenterol., 11.","DOI":"10.1186\/1471-230X-11-138"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1038\/nature08530","article-title":"Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43","volume":"461","author":"Maslowski","year":"2009","journal-title":"Nature"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/ueg2.12485","article-title":"Gut microbiota signatures in inflammatory bowel disease","volume":"12","author":"Vestergaard","year":"2024","journal-title":"United Eur. Gastroenterol. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.3945\/jn.113.179549","article-title":"Short-chain fatty acids activate AMP-activated protein kinase and ameliorate ethanol-induced intestinal barrier dysfunction in Caco-2 cell monolayers","volume":"143","author":"Elamin","year":"2013","journal-title":"J. Nutr."},{"key":"ref_43","first-page":"28","article-title":"Gingerenone A Attenuates Ulcerative Colitis via Targeting IL-17RA to Inhibit Inflammation and Restore Intestinal Barrier Function","volume":"11","author":"Liang","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1083\/jcb.200902153","article-title":"Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo","volume":"189","author":"Marchiando","year":"2010","journal-title":"J. Cell Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e178525","DOI":"10.1172\/jci.insight.178525","article-title":"Chlorination of epithelial tight junction proteins by neutrophil myeloperoxidase promotes barrier dysfunction and mucosal inflammation","volume":"9","author":"Cartwright","year":"2024","journal-title":"JCI Insight"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"van der Geize, R., Grommen, A.W., Hessels, G.I., Jacobs, A.A., and Dijkhuizen, L. (2011). The steroid catabolic pathway of the intracellular pathogen Rhodococcus equi is important for pathogenesis and a target for vaccine development. PLoS Pathog., 7.","DOI":"10.1371\/journal.ppat.1002181"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/mmi.12095","article-title":"FadD3 is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria","volume":"87","author":"Casabon","year":"2013","journal-title":"Mol. Microbiol."},{"key":"ref_48","unstructured":"National Center for Biotechnology Information (2026, February 09). PubChem Compound Summary for CID 21145116, 4-(2-Amino-3-hydroxyphenyl)-2,4-dioxobutanoate. PubChem, Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/4-_2-Amino-3-hydroxyphenyl_-2_4-dioxobutanoate."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Wang, J., An, W., Wang, Z., Zhao, Y., Han, B., Tao, H., Wang, J., and Wang, X. (2024). Vanillin has potent antibacterial, antioxidant, and anti-inflammatory activities in vitro and in mouse colitis induced by multidrug-resistant Escherichia coli. Antioxidants, 13.","DOI":"10.3390\/antiox13121544"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"7398","DOI":"10.1021\/jf3009553","article-title":"Arvelexin inhibits colonic inflammation by suppression of NF-\u03baB activation in dextran sulfate sodium-induced mice and TNF-\u03b1-induced colonic epithelial cells","volume":"60","author":"Cho","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_51","unstructured":"National Center for Biotechnology Information (2026, February 09). PubChem Compound Summary for CID 86289805, (2E)-19-hydroxynonadec-2-enoic Acid. PubChem, Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/2E_-19-hydroxynonadec-2-enoic-acid."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.bbrc.2007.12.188","article-title":"4-Hydroxydocosahexaenoic acid, a potent peroxisome proliferator-activated receptor gamma agonist alleviates the symptoms of DSS-induced colitis","volume":"367","author":"Yamamoto","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Aldars-Garc\u00eda, L., Gisbert, J.P., and Chaparro, M. (2021). Metabolomics Insights into Inflammatory Bowel Disease: A Comprehensive Review. Pharmaceuticals, 14.","DOI":"10.3390\/ph14111190"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e175","DOI":"10.1002\/imt2.175","article-title":"Wekemo Bioincloud: A user-friendly platform for meta-omics data analyses","volume":"3","author":"Gao","year":"2024","journal-title":"iMeta"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Tang, D., Chen, M., Huang, X., Zhang, G., Zeng, L., Zhang, G., Wu, S., and Wang, Y. (2023). SRplot: A free online platform for data visualization and graphing. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0294236"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Zhou, L., Ni, Z., Yu, J., Cheng, W., Cai, Z., and Yu, C. (2020). Correlation Between Fecal Metabolomics and Gut Microbiota in Obesity and Polycystic Ovary Syndrome. Front. Endocrinol., 11.","DOI":"10.3389\/fendo.2020.00628"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Hosseinkhani, F., Dubbelman, A.C., Karu, N., Harms, A.C., and Hankemeier, T. (2021). Towards Standards for Human Fecal Sample Preparation in Targeted and Untargeted LC-HRMS Studies. Metabolites, 11.","DOI":"10.3390\/metabo11060364"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/14\/6478\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:18:52Z","timestamp":1784647132000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/14\/6478"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,21]]},"references-count":57,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["ijms27146478"],"URL":"https:\/\/doi.org\/10.3390\/ijms27146478","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,21]]}}}