{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:59:48Z","timestamp":1785387588988,"version":"3.55.0"},"reference-count":58,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>This retrospective study characterizes the complex associations between serum 1H-NMR metabolomics, clinical nutritional status, and laboratory blood parameters in men with locally advanced head and neck squamous cell carcinoma (LA-HNSCC) in relation to 3-year disease-free survival (DFS). A total of 536 serum samples from 67 patients, collected weekly during radiotherapy or chemoradiotherapy, were analyzed to characterize the metabolic-nutritional landscape. The patients with 3-year DFS exhibited stronger biological links between elevated levels of serum lipids, phosphocholine, branched-chain amino acids (BCAAs), and clinical markers such as BMI, albumin, and prealbumin compared to those with disease recurrence. Furthermore, our findings highlight a novel pattern of disrupted correlations (metabolic decoupling) between body fat distribution, overall obesity, and lipid metabolism, as well as the links between BCAAs and nutritional status, in patients with disease recurrence. This observed breakdown of internal coordination suggests that the survival advantage associated with body fat arises not just from adiposity, but from the body\u2019s ability to metabolically coordinate fat and amino acid turnover with overall energy demands\u2014a trait known as metabolic flexibility. In conclusion, we hypothesize that disease recurrence in men with LA-HNSCC is driven by failure of this system, manifested as a breakdown in the coordination between metabolic and nutritional profiles. The preserved metabolic-nutritional axis between fat turnover and nutrient availability is associated with 3-year disease-free survival in this patient group, although more research is required to fully elucidate the underlying mechanisms.<\/jats:p>","DOI":"10.3390\/ijms27156715","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T16:11:56Z","timestamp":1785168716000},"page":"6715","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic and Clinical\u2013Nutritional Correlation Patterns in Relation to 3-Year Disease-Free Survival in Locally Advanced Head and Neck Squamous Cell Carcinoma: A Preliminary Analysis"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9460-837X","authenticated-orcid":false,"given":"\u0141ukasz","family":"Boguszewicz","sequence":"first","affiliation":[{"name":"Department of Medical Physics, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Hajduk-Biele\u0144","sequence":"additional","affiliation":[{"name":"1st Radiation and Clinical Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateusz","family":"Ciszek","sequence":"additional","affiliation":[{"name":"Department of Medical Physics, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Skorupa","sequence":"additional","affiliation":[{"name":"Department of Medical Physics, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"Mrochem-Kwarciak","sequence":"additional","affiliation":[{"name":"Analytics and Clinical Biochemistry Department, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Sk\u0142adowski","sequence":"additional","affiliation":[{"name":"1st Radiation and Clinical Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1611-8241","authenticated-orcid":false,"given":"Maria","family":"Sok\u00f3\u0142","sequence":"additional","affiliation":[{"name":"Department of Medical Physics, Maria Sklodowska-Curie National Research Institute of Oncology, Gliwice Branch, 44-102 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/s41572-020-00224-3","article-title":"Head and neck squamous cell carcinoma","volume":"6","author":"Johnson","year":"2020","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1056\/NEJMra1715715","article-title":"Head and Neck Cancer","volume":"382","author":"Chow","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3101","DOI":"10.1158\/0008-5472.CAN-23-3594","article-title":"Integrative multi-omics analyses identify molecular subtypes of head and neck squamous cell carcinoma with distinct therapeutic vulnerabilities","volume":"84","author":"Diao","year":"2024","journal-title":"Cancer Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2845","DOI":"10.1158\/1078-0432.CCR-22-3563","article-title":"Integrative Single-Cell and Bulk Transcriptomes Analyses Identify Intrinsic HNSCC Subtypes with Distinct Prognoses and Therapeutic Vulnerabilities","volume":"29","author":"Dai","year":"2023","journal-title":"Clin. Cancer Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ruud Kj\u00e6r, E.K., Jensen, J.S., Jakobsen, K.K., Lelkaitis, G., Wessel, I., von Buchwald, C., and Gr\u00f8nh\u00f8j, C. (2021). The Impact of Comorbidity on Survival in Patients with Head and Neck Squamous Cell Carcinoma: A Nationwide Case-Control Study Spanning 35 Years. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.617184"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"104561","DOI":"10.1016\/j.oraloncology.2019.104561","article-title":"Comorbidity in head and neck cancer: Is it associated with therapeutic delay, post-treatment mortality and survival in a population-based study?","volume":"102","author":"Stordeur","year":"2020","journal-title":"Oral Oncol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"101426","DOI":"10.1016\/j.tranon.2022.101426","article-title":"Therapeutic approaches for the treatment of head and neck squamous cell carcinoma-An update on clinical trials","volume":"21","author":"Goel","year":"2022","journal-title":"Transl. Oncol."},{"key":"ref_8","first-page":"e2045","article-title":"Developments and future prospects of personalized medicine in head and neck squamous cell carcinoma diagnoses and treatments","volume":"7","author":"Jayawickrama","year":"2024","journal-title":"Cancer Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.currproblcancer.2016.06.001","article-title":"Toxicities associated with head and neck cancer treatment and oncology-related clinical trials","volume":"40","author":"Villa","year":"2016","journal-title":"Curr. Probl. Cancer"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Hsieh, C.Y., Lein, M.Y., Yang, S.N., Wang, Y.C., Lin, Y.J., Lin, C.Y., Hua, C.H., Tsai, M.H., and Lin, C.C. (2020). Dose-dense TPF induction chemotherapy for locally advanced head and neck cancer: A phase II study. BMC Cancer, 20.","DOI":"10.1186\/s12885-020-07347-6"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"103986","DOI":"10.1016\/j.critrevonc.2023.103986","article-title":"Induction chemotherapy in locally advanced head and neck cancers, is there a best choice?","volume":"186","author":"Mahdavi","year":"2023","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s40537-022-00578-3","article-title":"Predicting clinical outcomes of radiotherapy for head and neck squamous cell carcinoma patients using machine learning algorithms","volume":"9","author":"Gangil","year":"2022","journal-title":"J. Big Data"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5985","DOI":"10.1038\/s41598-025-89053-6","article-title":"Survival and data-driven phenotypes in head and neck cancer","volume":"15","author":"Heinolainen","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8588","DOI":"10.1038\/s41598-023-34808-2","article-title":"Plasma metabolomics of oral squamous cell carcinomas based on NMR and MS approaches provides biomarker identification and survival prediction","volume":"13","author":"Polachini","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"102960","DOI":"10.1016\/j.ejon.2025.102960","article-title":"Prevalence and risk factors for critical weight loss among patients with head and neck cancer undergoing radiotherapy: A systematic review and meta-analysis","volume":"78","author":"Zhang","year":"2025","journal-title":"Eur. J. Oncol. Nurs."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.oraloncology.2018.05.027","article-title":"Impact of obesity on outcomes for patients with head and neck cancer","volume":"83","author":"Hicks","year":"2018","journal-title":"Oral Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1002\/hed.24760","article-title":"Body mass index and prognosis in patients with head and neck cancer","volume":"39","author":"Gama","year":"2017","journal-title":"Head Neck"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e2320513","DOI":"10.1001\/jamanetworkopen.2023.20513","article-title":"Association of Body Mass Index With Outcomes Among Patients With Head and Neck Cancer Treated With Chemoradiotherapy","volume":"6","author":"Ma","year":"2023","journal-title":"JAMA Netw. Open"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Liang, J., Li, L., Li, L., Zhou, X., Zhang, Z., Huang, Y., and Xiao, X. (2023). Lipid metabolism reprogramming in head and neck cancer. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1271505"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Boguszewicz, \u0141., Biele\u0144, A., Jarczewski, J.D., Ciszek, M., Skorupa, A., Sk\u0142adowski, K., and Soko\u0142, M. (2021). Molecular response to induc-tion chemotherapy and its correlation with treatment outcome in head and neck cancer patients by means of NMR-based metabolomics. BMC Cancer, 21.","DOI":"10.1186\/s12885-021-08137-4"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Miao, X., Wang, B., Chen, K., Ding, R., Wu, J., Pan, Y., Ji, P., Ye, B., and Xiang, M. (2022). Perspectives of lipid metabolism reprogramming in head and neck squamous cell carcinoma: An overview. Front. Oncol., 12.","DOI":"10.3389\/fonc.2022.1008361"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1038\/s41467-025-56675-3","article-title":"PPAR\u03b1-mediated lipid metabolism reprogramming supports anti-EGFR therapy resistance in head and neck squamous cell carcinoma","volume":"16","author":"Vignau","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/odi.14456","article-title":"Correlation analysis of plasma lipid profiles and the prognosis of head and neck squamous cell carcinoma","volume":"30","author":"Wang","year":"2024","journal-title":"Oral Dis."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wilms, T., Boldrup, L., Gu, X., Coates, P.J., Sgaramella, N., and Nylander, K. (2021). High Levels of Low-Density Lipoproteins Correlate with Improved Survival in Patients with Squamous Cell Carcinoma of the Head and Neck. Biomedicines, 9.","DOI":"10.3390\/biomedicines9050506"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/jop.12344","article-title":"Alteration of serum lipid profile and its prognostic value in head and neck squamous cell carcinoma","volume":"45","author":"Li","year":"2016","journal-title":"J. Oral Pathol. Med."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Boguszewicz, \u0141., Biele\u0144, A., Jarczewski, J.D., Ciszek, M., Skorupa, A., Mrochem-Kwarciak, J., Sk\u0142adowski, K., and Sok\u00f3\u0142, M. (2024). NMR-Based Metabolomics of Blood Serum in Predicting Response to Induction Chemotherapy in Head and Neck Cancer-A Preliminary Approach. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25147555"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"553","DOI":"10.2337\/diacare.5.5.553","article-title":"In defense of statistical significance with multivariate analysis","volume":"5","author":"Linn","year":"1982","journal-title":"Diabetes Care"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e4638","DOI":"10.1002\/nbm.4638","article-title":"Multivariate analysis of NMR-based metabolomic data","volume":"35","author":"Debik","year":"2022","journal-title":"NMR Biomed."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"17744","DOI":"10.1038\/srep17744","article-title":"Body mass index and risk of subtypes of head-neck cancer: The Netherlands Cohort Study","volume":"5","author":"Maasland","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S0002-9343(99)80220-4","article-title":"Body fat distribution, rather than overall adiposity, influences serum lipids and lipoproteins in healthy men independently of age","volume":"99","author":"Walton","year":"1995","journal-title":"Am. J. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1038\/sj.ijo.0800961","article-title":"Body mass index (weight\/height2) or percentage body fat by bioelectrical impedance analysis: Which variable better reflects serum lipid profile?","volume":"23","author":"Nagaya","year":"1999","journal-title":"Int. J. Obes. Relat. Metab. Disord."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/s43856-022-00208-2","article-title":"Adiposity and NMR-measured lipid and metabolic biomarkers among 30,000 Mexican adults","volume":"2","author":"Herrington","year":"2022","journal-title":"Commun. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"15530","DOI":"10.1038\/s41598-017-15346-0","article-title":"Potential Application of 1H NMR for Routine Serum Lipidome Analysis-Evaluation of Effects of Bariatric Surgery","volume":"7","author":"Mika","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Halczy-Kowalik, L., Drozd, A., Stachowska, E., Drozd, R., \u017babski, T., and Domaga\u0142a, W. (2019). Fatty acids distribution and content in oral squamous cell carcinoma tissue and its adjacent microenvironment. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0218246"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s12070-015-0829-4","article-title":"Alteration of Lipid Profile in Patients with Head and Neck Malignancy","volume":"68","author":"Poorey","year":"2016","journal-title":"Indian J. Otolaryngol. Head Neck Surg."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Astradsson, T., Sellberg, F., Berglund, D., Ehrsson, Y.T., and Laurell, G.F.E. (2019). Systemic Inflammatory Reaction in Patients With Head and Neck Cancer-An Explorative Study. Front. Oncol., 9.","DOI":"10.3389\/fonc.2019.01177"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s12944-024-02024-0","article-title":"Reprogramming of lipid metabolism in the tumor microenvironment: A strategy for tumor immunotherapy","volume":"23","author":"Wu","year":"2024","journal-title":"Lipids Health Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"5232","DOI":"10.1021\/pr200800w","article-title":"Magic angle spinning NMR-based metabolic profiling of head and neck squamous cell carcinoma tissues","volume":"10","author":"Somashekar","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kim, W., Lee, S., Seo, D., Kim, D., Kim, K., Kim, E., Kang, J., Seong, K.M., Youn, H., and Youn, B. (2019). Cellular Stress Responses in Radiotherapy. Cells, 8.","DOI":"10.3390\/cells8091105"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Kou, J., Li, W., Wang, Y., Su, X., and Zhang, H. (2025). BCAA metabolism in cancer progression and therapy resistance: The balance between fuel and cell signaling. Front. Pharmacol., 16.","DOI":"10.3389\/fphar.2025.1595176"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Xu, E., Ji, B., Jin, K., and Chen, Y. (2023). Branched-chain amino acids catabolism and cancer progression: Focus on therapeutic interven-tions. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1220638"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"102679","DOI":"10.1016\/j.ejon.2024.102679","article-title":"Impact of sarcopenia and obesity on overall survival in patients with head and neck cancer receiving radiotherapy: A longitudinal study","volume":"72","author":"Wang","year":"2024","journal-title":"Eur. J. Oncol. Nurs."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"103880","DOI":"10.1016\/j.numecd.2025.103880","article-title":"Serum branched-chain amino acids are mainly associated with body mass index and waist circumference","volume":"35","author":"Liu","year":"2025","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/s41387-022-00213-3","article-title":"Role of branched-chain amino acid metabolism in the pathogenesis of obesity and type 2 diabetes-related metabolic disturbances BCAA metabolism in type 2 diabetes","volume":"12","author":"Vanweert","year":"2022","journal-title":"Nutr. Diabetes"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s12986-018-0271-1","article-title":"Branched-chain amino acids in health and disease: Metabolism, alterations in blood plasma, and as supplements","volume":"15","year":"2018","journal-title":"Nutr. Metab."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"bvad178","DOI":"10.1210\/jendso\/bvad178","article-title":"Association of Elevated Serum Branched-chain Amino Acid Levels With Longitudinal Skeletal Muscle Loss","volume":"8","author":"Imai","year":"2024","journal-title":"J. Endocr. Soc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s12935-023-03014-5","article-title":"Enhancing prognostic accuracy in head and neck squamous cell carcinoma chemotherapy via a lipid metabolism-related clustered polygenic model","volume":"23","author":"Miao","year":"2023","journal-title":"Cancer Cell Int."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Qian, L., Qian, B., Xu, J., Yang, J., Wu, G., Zhao, Y., Liu, Q., Yuan, Z., Fan, Y., and Li, H. (2023). Clinical relevance of serum lipids in the carcinogenesis of oral squamous cell carcinoma. BMC Oral Health, 23.","DOI":"10.1186\/s12903-023-02859-6"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"107929","DOI":"10.1016\/j.oraloncology.2026.107929","article-title":"Sex-based immune differences influence tumor progression in head and neck squamous cell carcinoma","volume":"176","author":"Jagadeesha","year":"2026","journal-title":"Oral Oncol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Zohri, R., Hahn, L., Seyedi, N., Petersen, C., Ziemann, C., Abel, J., Kutz, L.M., Kr\u00fcll, A., Fl\u00fch, C., and Ehresmann, C. (2024). Nutritional Gender-Specific Differences in Head and Neck Cancer Patients Treated with (Chemo)Radiotherapy: Results from a Prospective Trial. Cancers, 16.","DOI":"10.3390\/cancers16234080"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1007\/s12094-023-03112-w","article-title":"Sex differences in the diagnosis, treatment and prognosis of cancer: The rationale for an individualised approach","volume":"25","author":"Vera","year":"2023","journal-title":"Clin. Transl. Oncol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"oyag167","DOI":"10.1093\/oncolo\/oyag167","article-title":"Female sex is associated with worse survival in laryngeal head and neck squamous cell carcinoma","volume":"31","author":"Yao","year":"2026","journal-title":"Oncologist"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"19160216251318238","DOI":"10.1177\/19160216251318238","article-title":"Sex Differences in Head and Neck Carcinoma-A Retrospective Study on 1115 Head and Neck Carcinoma Patients in Austria","volume":"54","author":"Kofler","year":"2025","journal-title":"J. Otolaryngol. Head Neck Surg."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.rpor.2015.05.010","article-title":"Effect of radiotherapy delay in overall treatment time on local control and survival in head and neck cancer: Review of the literature","volume":"20","author":"Azinovic","year":"2015","journal-title":"Rep. Pract. Oncol. Radiother."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2692","DOI":"10.1038\/nprot.2007.376","article-title":"Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts","volume":"2","author":"Beckonert","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0169-7439(01)00155-1","article-title":"PLS-regression: A basic tool of chemometrics","volume":"58","author":"Wold","year":"2001","journal-title":"Chemom. Intell. Lab. Syst."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/cem.695","article-title":"Orthogonal projections to latent structures (O-PLS)","volume":"16","author":"Trygg","year":"2002","journal-title":"J. Chemom."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Boguszewicz, \u0141., Biele\u0144, A., Ciszek, M., Skorupa, A., Mrochem-Kwarciak, J., Sk\u0142adowski, K., and Sok\u00f3\u0142, M. (2023). Metabolomic Insight into Implications of Induction Chemotherapy Followed by Concomitant Chemoradiotherapy in Locally Advanced Head and Neck Cancer. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25010188"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/15\/6715\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:22:13Z","timestamp":1785385333000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/15\/6715"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,27]]},"references-count":58,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ijms27156715"],"URL":"https:\/\/doi.org\/10.3390\/ijms27156715","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,27]]}}}