{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T09:46:42Z","timestamp":1785923202790,"version":"3.56.0"},"reference-count":52,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"crossref","award":["20130279"],"award-info":[{"award-number":["20130279"]}],"id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"crossref"}]},{"award":["20130279"],"award-info":[{"award-number":["20130279"]}],"id":[{"id":"https:\/\/ror.org\/00c5s4a53","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>The skipjack tuna (Katsuwonus pelamis) is one of the least commercially exploited tuna globally. The possible medicinal benefits of K. pelamis extract have not been explored. We investigated the 70% ethanol (EtOH) soluble fractions of skipjack tuna heart for the inhibition of protein-tyrosine phosphatase 1B (PTP1B) and \u03b1-glucosidase. The dichloromethane (CH2Cl2) fraction significantly inhibited the activities of PTP1B and \u03b1-glucosidase. Repeated column chromatography of the active CH2Cl2 fraction based on bioactivity-guided fractionation yielded cholesterol, cholesteryl myristate, and sn-1,3-dipalmitoyl-2-oleoylglycerol; the latter significantly inhibited PTP1B and \u03b1-glucosidase. Kinetic study revealed that sn-1,3-dipalmitoyl-2-oleoylglycerol showed mixed-type inhibition against PTP1B. Docking simulations showed that sn-1,3-dipalmitoyl-2-oleoylglycerol selectively inhibited PTP1B and \u03b1-glucosidase by targeting its active site and exhibited good binding affinity, with a docking score of \u22127.4 and \u22127.1 kcal\/mol, respectively. Moreover, 70% EtOH, CH2Cl2 and EtOAc fractions, and sn-1,3-dipalmitoyl-2-oleoyl glycerol significantly inhibited ONOO\u2212mediated albumin nitration. These results implicate tuna heart extract as a potential functional food ingredient for the prevention and treatment of diabetes and its associated complications.<\/jats:p>","DOI":"10.3390\/ijms27156914","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:57:56Z","timestamp":1785761876000},"page":"6914","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Activity-Guided Isolation of sn-1,3-Dipalmitoyl-2-oleoylglycerol as Protein Tyrosine Phosphatase 1B and \u03b1-Glucosidase Inhibitor from Skipjack Tuna (Katsuwonus pelamis)"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1488-5711","authenticated-orcid":false,"given":"Md Yousof","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Clinical Neurosciences, Hotchkiss Brain Institute and Alberta Children\u2019s Hospital Research Institute, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada"},{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da Hye","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7212-5572","authenticated-orcid":false,"given":"Hee Jin","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5954-6935","authenticated-orcid":false,"given":"Taek Jeong","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9034-8868","authenticated-orcid":false,"given":"Jae Sue","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.2337\/diacare.27.5.1047","article-title":"Global prevalence of diabetes","volume":"27","author":"Wild","year":"2004","journal-title":"Diabetes Care"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1001\/archinternmed.2011.400","article-title":"Encounter frequency and serum glucose level, blood pressure, and cholesterol level control in patients with diabetes mellitus","volume":"171","author":"Morrison","year":"2011","journal-title":"Arch. 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