{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T13:59:11Z","timestamp":1785765551876,"version":"3.56.0"},"reference-count":54,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pavlov Center\u2014Integrative Physiology to Medicine, High-tech Healthcare and Technologies of Stress Resistance","award":["075-15-2020-921"],"award-info":[{"award-number":["075-15-2020-921"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>The present study aimed to elucidate whether the metabolic effects of the probiotic bacterium Hafnia alvei 4597 depend on melanocortin receptor (MCR) signaling. The response to a 3-week intragastric treatment with the H. alvei bacterial suspension or total protein extract was compared between genetically similar mouse sub-strains but with different MCR sensitivity: KK and KK.Cg-Ay\/a (KK-Ay)\u2014the latter overproducing Agouti protein. Treatment of KK mice with H. alvei protein extract stimulated energy expenditure and carbohydrate oxidation but reduced lipid oxidation, leptin level, pancreatic weight, and hypothalamic insulin and leptin receptor mRNA expression. Live bacteria in KK mice reduced food intake and stimulated hypothalamic mRNA expression of proopiomelanocortin, Agouti-related peptide, and insulin receptors. In the sub-strain KK-Ay, probiotics had no effect on the aforementioned metabolic parameters. H. alvei-based probiotics improved glucose tolerance and decreased body fat and liver glycogen in both mouse sub-strains. Activation of MC4R by H. alvei protein extract was revealed by in vitro study using \u03b2-arrestin recruitment. These findings confirm beneficial effects of the H. alvei bacteria and show that, for several parameters, the bacterial protein extract may be more effective than bacterial suspension. Differential responses to the treatment between the mouse sub-strains, particularly in energy metabolism, as well as in vitro data, indicate that the effects of H. alvei are mediated by MCR signaling.<\/jats:p>","DOI":"10.3390\/ijms27156963","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:57:56Z","timestamp":1785761876000},"page":"6963","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Melanocortin Receptor Signaling Modulates Anti-Obesity and Glucose Tolerance Effects of Hafnia alvei 4597 Probiotic Strain in Mice"],"prefix":"10.3390","volume":"27","author":[{"given":"Vasiliy A.","family":"Zolotarev","sequence":"first","affiliation":[{"name":"Pavlov Institute of Physiology, Russian Academy of Sciences, Saint-Petersburg 199034, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir O.","family":"Murovets","sequence":"additional","affiliation":[{"name":"Pavlov Institute of Physiology, Russian Academy of Sciences, Saint-Petersburg 199034, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egor A.","family":"Sozontov","sequence":"additional","affiliation":[{"name":"Pavlov Institute of Physiology, Russian Academy of Sciences, Saint-Petersburg 199034, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia L.","family":"Sepp","sequence":"additional","affiliation":[{"name":"Pavlov Institute of Physiology, Russian Academy of Sciences, Saint-Petersburg 199034, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina A.","family":"Lukina","sequence":"additional","affiliation":[{"name":"Pavlov Institute of Physiology, Russian Academy of Sciences, Saint-Petersburg 199034, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raisa P.","family":"Khropycheva","sequence":"additional","affiliation":[{"name":"Pavlov Institute of Physiology, Russian Academy of Sciences, Saint-Petersburg 199034, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Thomas","sequence":"additional","affiliation":[{"name":"Inserm UMR1239 Laboratory, University of Rouen Normandie, 76130 Mont-Saint-Aignan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2491-4945","authenticated-orcid":false,"given":"Sergue\u00ef O.","family":"Fetissov","sequence":"additional","affiliation":[{"name":"Inserm UMR1239 Laboratory, University of Rouen Normandie, 76130 Mont-Saint-Aignan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ramos-Vivas, J., Tapia, O., Elexpuru-Zabaleta, M., Pifarre, K.T., Armas Diaz, Y., Battino, M., and Giampieri, F. 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