{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T04:44:07Z","timestamp":1786164247176,"version":"build-2736575974"},"reference-count":53,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Postgraduate Studies and Scientific Research, Qassim University","award":["QU-APC-2026"],"award-info":[{"award-number":["QU-APC-2026"]}]},{"award":["QU-APC-2026"],"award-info":[{"award-number":["QU-APC-2026"]}],"id":[{"id":"https:\/\/ror.org\/01wsfe280","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Phosphatidylinositol 5-phosphate 4-kinase type 2 alpha (PIP4K2A) is a key oncogenic driver that regulates the PI5P\/PIP2 axis to promote metastatic migration in breast cancer. This study aimed to investigate the therapeutic potential of a crude extract from Tamarix aphylla against breast cancer progression and identify its primary active constituents. The crude extract was initially evaluated against MDA-MB-231 and MCF7 breast cancer cell lines using wound healing assays. Bioassay-guided isolation and screening were deployed to isolate individual components, and the most potent lead compound was structurally characterized using preparative HPLC and FTIR. To analyze its interaction with PIP4K2A, in silico molecular docking, MM\/GBSA calculations, and 200 ns molecular dynamics simulations were conducted. In vitro validation was subsequently performed via dose-dependent cytotoxicity assays, scratch assays, single-cell tracking, and RT-qPCR expression analysis. Quercetin was identified as the most potent lead inhibitor against PIP4K2A. Computational modeling revealed that quercetin binds tightly within the PIP4K2A ATP-binding pocket, yielding a superior binding affinity of \u221210.77 kcal\/mol and enhanced thermodynamic stability (\u0394GMM\/GBSA = \u221242.6 \u00b1 2.1 kcal\/mol) compared to the native ligand (\u0394G MM\/GBSA = \u221223.3 \u00b1 1.8 kcal\/mol). Molecular dynamics simulations confirmed an induced-fit structural transition that locked the complex into an ultra-stable conformation within a deep global energy minimum basin (\u221210.8 kcal\/mol). In vitro assays demonstrated dose-dependent cytotoxicity, with aggressive triple-negative MDA-MB-231 cells exhibiting higher sensitivity (IC50 = 82.23 \u00b5g\/mL) than luminal MCF7 cells (IC50 = 97.14 \u00b5g\/mL). Furthermore, scratch and single-cell tracking assays showed a profound suppression of migration speed and wound closure (reduced to ~40%), while RT-qPCR revealed a near-complete transcriptional knockdown of PIP4K2A mRNA expression (down to 0.025-fold). Collectively, these findings elucidate a unique dual-action mechanism for Tamarix aphylla-derived quercetin\u2014characterized by both direct competitive enzymatic inhibition and downstream transcriptional silencing\u2014positioning it as a promising therapeutic scaffold for targeted anti-metastatic breast cancer interventions.<\/jats:p>","DOI":"10.3390\/ijms27157063","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T14:45:47Z","timestamp":1786027547000},"page":"7063","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Anti-Breast Cancer Effects of Tamarix aphylla-Derived Quercetin and In Silico Insights into Its Targeting of PIP4K2A"],"prefix":"10.3390","volume":"27","author":[{"given":"Dhurgham","family":"Al-Fahad","sequence":"first","affiliation":[{"name":"Department of Pathological Analysis, College of Science, University of Thi-Qar, Nasiriyah 64001, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahraa Naeem","family":"Hashim","sequence":"additional","affiliation":[{"name":"Department of Pathological Analysis, College of Science, University of Thi-Qar, Nasiriyah 64001, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suliman A.","family":"Almahmoud","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2927-8167","authenticated-orcid":false,"given":"Faizul","family":"Azam","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah 51452, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/s41392-024-02108-4","article-title":"Breast cancer: Pathogenesis and treatments","volume":"10","author":"Xiong","year":"2025","journal-title":"Signal Transduct. 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