{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:28:39Z","timestamp":1786357719295,"version":"build-2736575974"},"reference-count":66,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Sulforaphane (SFN), an isothiocyanate produced from glucoraphanin in cruciferous vegetables, has been associated with the regulation of lipid storage, fatty acid oxidation, and autophagic lipid turnover. Therefore, in the current narrative review, AMPK-centered mechanisms were critically evaluated by distinguishing pathway association from experimentally demonstrated AMPK dependence. In cell and animal models, SFN treatment was associated with ACC phosphorylation, inhibition of mTORC1-SREBP signaling, CPT-1-related fatty acid oxidation, PGC-1\u03b1\/PPAR-\u03b1 transcriptional regulation, and ULK1-mediated lipophagy. However, most studies measured AMPK phosphorylation without pharmacological or genetic suppression, and one adipocyte study showed decreased AMPK phosphorylation during SFN-induced lipolysis. These findings indicate that SFN signaling can vary depending on the model, dose, and measured endpoint. Human studies have also shown highly variable SFN exposure and limited or inconsistent effects on lipid and glycemic outcomes, but AMPK-mediated lipid remodeling has not been directly demonstrated in target tissues. In addition, food matrix, myrosinase activity, dose, gut microbiota, and SFN-NIT formation can influence systemic exposure. Therefore, AMPK may be considered an important component of a broader SFN-responsive network rather than an exclusive mechanism. Further standardized human studies are required to evaluate quantified SFN exposure, lipid-related outcomes, tissue-relevant pathway biomarkers, and long-term safety.<\/jats:p>","DOI":"10.3390\/ijms27167138","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T09:24:53Z","timestamp":1786353893000},"page":"7138","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulation of Obesity-Related Lipid Metabolism by Sulforaphane Through AMPK-Centered Molecular Networks"],"prefix":"10.3390","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5979-2626","authenticated-orcid":false,"given":"Uyory","family":"Choe","sequence":"first","affiliation":[{"name":"Department of Food and Nutrition, College of Biomedical and Health Science, Konkuk University, 268 Chungwon-daero, Chungju-si 27478, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lv, Q., Li, X., Fan, B., Zhu, C., and Chen, Z. (2022). The Cellular and Subcellular Organization of the Glucosinolate\u2013Myrosinase System against Herbivores and Pathogens. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23031577"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Olayanju, J.B., Bozic, D., Naidoo, U., and Sadik, O.A. (2024). A Comparative Review of Key Isothiocyanates and Their Health Benefits. Nutrients, 16.","DOI":"10.3390\/nu16060757"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"107107","DOI":"10.1016\/j.phrs.2024.107107","article-title":"Isothiocyanates in Medicine: A Comprehensive Review on Phenylethyl-, Allyl-, and Benzyl-Isothiocyanates","volume":"201","author":"Hoch","year":"2024","journal-title":"Pharmacol. Res."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ruhee, R.T., and Suzuki, K. (2020). The Integrative Role of Sulforaphane in Preventing Inflammation, Oxidative Stress and Fatigue: A Review of a Potential Protective Phytochemical. Antioxidants, 9.","DOI":"10.3390\/antiox9060521"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/S0140-6736(25)00355-1","article-title":"Global, Regional, and National Prevalence of Adult Overweight and Obesity, 1990\u20132021, with Forecasts to 2050: A Forecasting Study for the Global Burden of Disease Study 2021","volume":"405","author":"Ng","year":"2025","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1016\/S0140-6736(23)02750-2","article-title":"Worldwide Trends in Underweight and Obesity from 1990 to 2022: A Pooled Analysis of 3663 Population-Representative Studies with 222 Million Children, Adolescents, and Adults","volume":"403","author":"Phelps","year":"2024","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"\u00c7ubuk, M., P\u0131nar, A.A., S\u00fcleyman, B., and Ta\u015f, N.G. (2026). Sulforaphane Against the Metabolic Consequences of a High-Glycemic-Index Diet: Protective and Therapeutic Mechanisms Associated with Obesity and Insulin Resistance. Nutrients, 18.","DOI":"10.3390\/nu18040574"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1039\/b502990b","article-title":"The Glucosinolate\u2013Myrosinase System. New Insights into Enzyme\u2013Substrate Interactions by Use of Simplified Inhibitors","volume":"3","author":"Bourderioux","year":"2005","journal-title":"Org. Biomol. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1631\/jzus.B0920051","article-title":"Effects of Different Cooking Methods on Health-Promoting Compounds of Broccoli","volume":"10","author":"Yuan","year":"2009","journal-title":"J. Zhejiang Univ. Sci. B"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Bouranis, J.A., Beaver, L.M., and Ho, E. (2021). Metabolic Fate of Dietary Glucosinolates and Their Metabolites: A Role for the Microbiome. Front. Nutr., 8.","DOI":"10.3389\/fnut.2021.748433"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"11519","DOI":"10.1021\/acs.jafc.8b03096","article-title":"The Science behind Microgreens as an Exciting New Food for the 21st Century","volume":"66","author":"Choe","year":"2018","journal-title":"J. Agric. Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1038\/oby.2011.388","article-title":"Sulforaphane Inhibits Mitotic Clonal Expansion during Adipogenesis Through Cell Cycle Arrest","volume":"20","author":"Choi","year":"2012","journal-title":"Obesity"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.bbrc.2012.08.107","article-title":"Sulforaphane Induced Adipolysis via Hormone Sensitive Lipase Activation, Regulated by AMPK Signaling Pathway","volume":"426","author":"Lee","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jnutbio.2013.10.007","article-title":"Sulforaphane Attenuates Obesity by Inhibiting Adipogenesis and Activating the AMPK Pathway in Obese Mice","volume":"25","author":"Choi","year":"2014","journal-title":"J. Nutr. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Men, X., Han, X., Lee, S.-J., Oh, G., Park, K.-T., Han, J.-K., Choi, S.-I., and Lee, O.-H. (2022). Anti-Obesogenic Effects of Sulforaphane-Rich Broccoli (Brassica oleracea Var. Italica) Sprouts and Myrosinase-Rich Mustard (Sinapis alba L.) Seeds In Vitro and In Vivo. Nutrients, 14.","DOI":"10.3390\/nu14183814"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"109017","DOI":"10.1016\/j.jnutbio.2022.109017","article-title":"Sulforaphane Induces Lipophagy Through the Activation of AMPK-mTOR-ULK1 Pathway Signaling in Adipocytes","volume":"106","author":"Masuda","year":"2022","journal-title":"J. Nutr. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"157935","DOI":"10.1016\/j.phymed.2026.157935","article-title":"Sulforaphane-Rich Aqueous Broccoli Seed Extract Suppresses Diet-Induced Obesity via 5-HT2A\/AMPK Signaling in Mice","volume":"153","author":"Jeon","year":"2026","journal-title":"Phytomedicine"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.diabres.2012.01.009","article-title":"Broccoli Sprouts Powder Could Improve Serum Triglyceride and Oxidized LDL\/LDL-Cholesterol Ratio in Type 2 Diabetic Patients: A Randomized Double-Blind Placebo-Controlled Clinical Trial","volume":"96","author":"Bahadoran","year":"2012","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1002\/mnfr.201400863","article-title":"Diet Rich in High Glucoraphanin Broccoli Reduces Plasma LDL Cholesterol: Evidence from Randomised Controlled Trials","volume":"59","author":"Armah","year":"2015","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"eaah4477","DOI":"10.1126\/scitranslmed.aah4477","article-title":"Sulforaphane Reduces Hepatic Glucose Production and Improves Glucose Control in Patients with Type 2 Diabetes","volume":"9","author":"Axelsson","year":"2017","journal-title":"Sci. Transl. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1002\/mnfr.201400674","article-title":"Absorption and Chemopreventive Targets of Sulforaphane in Humans Following Consumption of Broccoli Sprouts or a Myrosinase-Treated Broccoli Sprout Extract","volume":"59","author":"Atwell","year":"2015","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1038\/s41564-025-01932-w","article-title":"Effect of Broccoli Sprout Extract and Baseline Gut Microbiota on Fasting Blood Glucose in Prediabetes: A Randomized, Placebo-Controlled Trial","volume":"10","author":"Dwibedi","year":"2025","journal-title":"Nat. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1700991","DOI":"10.1002\/mnfr.201700991","article-title":"Gut Glucosinolate Metabolism and Isothiocyanate Production","volume":"62","author":"Narbad","year":"2018","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"102878","DOI":"10.1016\/j.redox.2023.102878","article-title":"Sulforaphane Rewires Central Metabolism to Support Antioxidant Response and Achieve Glucose Homeostasis","volume":"67","author":"Bernuzzi","year":"2023","journal-title":"Redox Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s11033-025-10358-w","article-title":"Protective Role of Sulforaphane in Lipid Metabolism-Related Diseases","volume":"52","author":"Zhao","year":"2025","journal-title":"Mol. Biol. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Barba, F.J., Nikmaram, N., Roohinejad, S., Khelfa, A., Zhu, Z., and Koubaa, M. (2016). Bioavailability of Glucosinolates and Their Breakdown Products: Impact of Processing. Front. Nutr., 3.","DOI":"10.3389\/fnut.2016.00024"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1079\/BJN2003900","article-title":"Influence of Plant and Bacterial Myrosinase Activity on the Metabolic Fate of Glucosinolates in Gnotobiotic Rats","volume":"90","author":"Rouzaud","year":"2003","journal-title":"Br. J. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sikorska-Zimny, K., and Beneduce, L. (2021). The Metabolism of Glucosinolates by Gut Microbiota. Nutrients, 13.","DOI":"10.3390\/nu13082750"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Bouranis, J.A., Beaver, L.M., Choi, J., Wong, C.P., Jiang, D., Sharpton, T.J., Stevens, J.F., and Ho, E. (2021). Composition of the Gut Microbiome Influences Production of Sulforaphane-Nitrile and Iberin-Nitrile from Glucosinolates in Broccoli Sprouts. Nutrients, 13.","DOI":"10.3390\/nu13093013"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2300286","DOI":"10.1002\/mnfr.202300286","article-title":"Sulforaphane and Sulforaphane-Nitrile Metabolism in Humans Following Broccoli Sprout Consumption: Inter-individual Variation, Association with Gut Microbiome Composition, and Differential Bioactivity","volume":"68","author":"Bouranis","year":"2024","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nrm3311","article-title":"AMPK: A Nutrient and Energy Sensor That Maintains Energy Homeostasis","volume":"13","author":"Hardie","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.molcel.2017.05.032","article-title":"AMPK: Mechanisms of Cellular Energy Sensing and Restoration of Metabolic Balance","volume":"66","author":"Garcia","year":"2017","journal-title":"Mol. Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.yjmcc.2014.09.022","article-title":"Sulforaphane Prevents the Development of Cardiomyopathy in Type 2 Diabetic Mice Probably by Reversing Oxidative Stress-Induced Inhibition of LKB1\/AMPK Pathway","volume":"77","author":"Zhang","year":"2014","journal-title":"J. Mol. Cell. Cardiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"176477","DOI":"10.1016\/j.ejphar.2024.176477","article-title":"Sulforaphane\u2019s Role in Redefining Autophagic Responses in Depression Associated with Polycystic Ovarian Syndrome: Unveiling the SIRT1\/AMPK\/LKB1 Pathway Connection","volume":"969","author":"Kamel","year":"2024","journal-title":"Eur. J. Pharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.cmet.2005.06.005","article-title":"Ca2+\/Calmodulin-Dependent Protein Kinase Kinase-\u03b2 Acts Upstream of AMP-Activated Protein Kinase in Mammalian Cells","volume":"2","author":"Woods","year":"2005","journal-title":"Cell Metab."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cmet.2005.05.009","article-title":"Calmodulin-Dependent Protein Kinase Kinase-\u03b2 Is an Alternative Upstream Kinase for AMP-Activated Protein Kinase","volume":"2","author":"Hawley","year":"2005","journal-title":"Cell Metab."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2469","DOI":"10.1042\/CS20191088","article-title":"Sulforaphane Prevents Type 2 Diabetes-Induced Nephropathy via AMPK-Mediated Activation of Lipid Metabolic Pathways and Nrf2 Antioxidative Function","volume":"134","author":"Li","year":"2020","journal-title":"Clin. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"15126","DOI":"10.1021\/acs.jafc.2c06311","article-title":"Sulforaphane Ameliorates Hepatic Lipid Metabolism via Modulating Lipophagy In Vivo and In Vitro","volume":"70","author":"Lei","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Aranda-Rivera, A.K., Amador-Mart\u00ednez, I., Aparicio-Trejo, O.E., Le\u00f3n-Contreras, J.C., Hern\u00e1ndez-Pando, R., Saavedra, E., Garc\u00eda-Arroyo, F.E., Pedraza-Chaverri, J., S\u00e1nchez-Lozada, L.G., and Tapia, E. (2025). Sulforaphane Restores Mitochondrial \u03b2-Oxidation and Reduces Renal Lipid Accumulation in a Model of Releasing Unilateral Ureteral Obstruction. Antioxidants, 14.","DOI":"10.3390\/antiox14030288"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1432-1033.1997.00001.x","article-title":"The Mitochondrial Carnitine Palmitoyltransferase System\u2014From Concept to Molecular Analysis","volume":"244","author":"McGarry","year":"1997","journal-title":"Eur. J. Biochem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"113614","DOI":"10.1016\/j.yexcr.2023.113614","article-title":"AMP-Activated Protein Kinase: An Energy Sensor and Survival Mechanism in the Reinstatement of Metabolic Homeostasis","volume":"428","author":"Sharma","year":"2023","journal-title":"Exp. Cell Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2727","DOI":"10.26599\/FSHW.2022.9250220","article-title":"Sulforaphane Ameliorates Non-Alcoholic Steatohepatitis by KLF4-Mediated Macrophage M2 Polarization","volume":"13","author":"Huang","year":"2024","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1868","DOI":"10.1128\/MCB.20.5.1868-1876.2000","article-title":"The Coactivator PGC-1 Cooperates with Peroxisome Proliferator-Activated Receptor \u03b1 in Transcriptional Control of Nuclear Genes Encoding Mitochondrial Fatty Acid Oxidation Enzymes","volume":"20","author":"Vega","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.molmet.2014.02.002","article-title":"Integrated Physiology and Systems Biology of PPAR\u03b1","volume":"3","author":"Kersten","year":"2014","journal-title":"Mol. Metab."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Hajri, T., Zaiou, M., Fungwe, T.V., Ouguerram, K., and Besong, S. (2021). Epigenetic Regulation of Peroxisome Proliferator-Activated Receptor Gamma Mediates High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease. Cells, 10.","DOI":"10.3390\/cells10061355"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1016\/j.jcmgh.2021.01.012","article-title":"Transcriptional Regulation of Metabolic Pathways via Lipid-Sensing Nuclear Receptors PPARs, FXR, and LXR in NASH","volume":"11","author":"Cariello","year":"2021","journal-title":"Cell. Mol. Gastroenterol. Hepatol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Abu Shelbayeh, O., Arroum, T., Morris, S., and Busch, K.B. (2023). PGC-1\u03b1 Is a Master Regulator of Mitochondrial Lifecycle and ROS Stress Response. Antioxidants, 12.","DOI":"10.3390\/antiox12051075"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.ceb.2017.02.012","article-title":"mTORC1 Signaling and the Metabolic Control of Cell Growth","volume":"45","author":"Manning","year":"2017","journal-title":"Curr. Opin. Cell Biol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Liu, Y., Wang, Y., Zhou, J., Li, H., Liu, C., Zhong, B., Liu, J., Liu, L., Zhang, L., and Sun, L. (2025). Restoring Metabolic Flexibility: Targeting Organelle Interaction Networks in the Pathogenesis and Therapy of MASLD. Front. Cell Dev. Biol., 13.","DOI":"10.3389\/fcell.2025.1718799"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.molcel.2008.03.003","article-title":"AMPK Phosphorylation of Raptor Mediates a Metabolic Checkpoint","volume":"30","author":"Gwinn","year":"2008","journal-title":"Mol. Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1172\/JCI0215593","article-title":"SREBPs: Activators of the Complete Program of Cholesterol and Fatty Acid Synthesis in the Liver","volume":"109","author":"Horton","year":"2002","journal-title":"J. Clin. Investig."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e33670","DOI":"10.1016\/j.heliyon.2024.e33670","article-title":"Recent Advances in Anti-Inflammation via AMPK Activation","volume":"10","author":"Xu","year":"2024","journal-title":"Heliyon"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"102049","DOI":"10.1016\/j.gendis.2026.102049","article-title":"Lipophagy: A Key Regulator in Oxidative Stress and Metabolic Disorders","volume":"13","author":"Zhao","year":"2026","journal-title":"Genes Dis."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1080\/15548627.2022.2117513","article-title":"Eating Your Own Fat to Stay Fit: Lipophagy Sustains Lymphangiogenesis","volume":"19","author":"Mece","year":"2023","journal-title":"Autophagy"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1126\/science.1196371","article-title":"Phosphorylation of ULK1 (hATG1) by AMP-Activated Protein Kinase Connects Energy Sensing to Mitophagy","volume":"331","author":"Egan","year":"2011","journal-title":"Science"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1038\/ncb2152","article-title":"AMPK and mTOR Regulate Autophagy through Direct Phosphorylation of Ulk1","volume":"13","author":"Kim","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"ref_57","first-page":"1178","article-title":"Breaking Fat: The Regulation and Mechanisms of Lipophagy","volume":"1862","author":"Schulze","year":"2017","journal-title":"Biochim. Biophys. Acta (BBA)\u2014Mol. Cell Biol. Lipids"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Fahey, J.W., Holtzclaw, W.D., Wehage, S.L., Wade, K.L., Stephenson, K.K., and Talalay, P. (2015). Sulforaphane Bioavailability from Glucoraphanin-Rich Broccoli: Control by Active Endogenous Myrosinase. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0140963"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1700911","DOI":"10.1002\/mnfr.201700911","article-title":"Bioavailability of Glucoraphanin and Sulforaphane from High-Glucoraphanin Broccoli","volume":"62","author":"Sivapalan","year":"2018","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1017\/S0007114511000274","article-title":"Variation of Glucoraphanin Metabolism In Vivo and Ex Vivo by Human Gut Bacteria","volume":"106","author":"Li","year":"2011","journal-title":"Br. J. Nutr."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Dmytriv, T.R., Lushchak, O., and Lushchak, V.I. (2025). Glucoraphanin Conversion into Sulforaphane and Related Compounds by Gut Microbiota. Front. Physiol., 16.","DOI":"10.3389\/fphys.2025.1497566"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1207\/s15327914nc5501_7","article-title":"Safety, Tolerance, and Metabolism of Broccoli Sprout Glucosinolates and Isothiocyanates: A Clinical Phase I Study","volume":"55","author":"Shapiro","year":"2006","journal-title":"Nutr. Cancer"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1158\/1940-6207.CAPR-14-0103","article-title":"Rapid and Sustainable Detoxication of Airborne Pollutants by Broccoli Sprout Beverage: Results of a Randomized Clinical Trial in China","volume":"7","author":"Egner","year":"2014","journal-title":"Cancer Prev. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fct.2019.02.004","article-title":"Broccoli Sprout Beverage Is Safe for Thyroid Hormonal and Autoimmune Status: Results of a 12-Week Randomized Trial","volume":"126","author":"Chartoumpekis","year":"2019","journal-title":"Food Chem. Toxicol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.taap.2012.10.029","article-title":"Sulforaphane Is Not an Effective Antagonist of the Human Pregnane X-Receptor In Vivo","volume":"266","author":"Poulton","year":"2013","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"6743","DOI":"10.1021\/jf2050284","article-title":"Impact of Thermal Processing on Sulforaphane Yield from Broccoli (Brassica oleracea L. ssp. Italica)","volume":"60","author":"Wang","year":"2012","journal-title":"J. Agric. Food Chem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7138\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T09:34:38Z","timestamp":1786354478000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/27\/16\/7138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,9]]},"references-count":66,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["ijms27167138"],"URL":"https:\/\/doi.org\/10.3390\/ijms27167138","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,9]]}}}