{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:28:40Z","timestamp":1786357720716,"version":"build-2736575974"},"reference-count":26,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T00:00:00Z","timestamp":1786233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100013302","name":"King Abdullah International Medical Research Center","doi-asserted-by":"publisher","award":["NRC24\/068"],"award-info":[{"award-number":["NRC24\/068"]}],"id":[{"id":"10.13039\/501100013302","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013302","name":"King Abdullah International Medical Research Center","doi-asserted-by":"publisher","award":["RC18\/173"],"award-info":[{"award-number":["RC18\/173"]}],"id":[{"id":"10.13039\/501100013302","id-type":"DOI","asserted-by":"publisher"}]},{"award":["NRC24\/068"],"award-info":[{"award-number":["NRC24\/068"]}],"id":[{"id":"https:\/\/ror.org\/009p8zv69","id-type":"ROR","asserted-by":"publisher"}]},{"award":["RC18\/173"],"award-info":[{"award-number":["RC18\/173"]}],"id":[{"id":"https:\/\/ror.org\/009p8zv69","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int. J. Mol. Sci.","IJMS"],"abstract":"<jats:p>Brain cancer is a highly aggressive disease with limited treatment options, highlighting the need for reliable preclinical models for drug discovery. This study aimed to isolate and characterize Saudi patient-derived primary brain cancer cells and assess the anticancer activity of novel compounds developed in-house. Sixteen tumor samples from Saudi patients were processed to establish primary brain cancer cultures and One Normal Tissue (Control). The cells were successfully isolated and maintained under optimized conditions, with their morphology and growth characteristics monitored. Molecular analysis confirmed the expression of key tumor and neural markers. The anticancer activity of selected compounds, KCO69, KCO70, and KCO129, was tested at various concentrations using the MTT and CellTiter-Glo Luminescent Cell Viability Assay. All compounds caused a concentration-dependent reduction in cell viability, with the strongest effects seen at 25 \u00b5M. Among them, compound 70 showed the most significant antiproliferative activity, while compounds KCO69 and KCO129 exhibited moderate effects. Variability in treatment response among cultures reflected the inherent heterogeneity of patient-derived tumors. Overall, establishing primary brain cancer cell models from Saudi patients offers a valuable platform for preclinical drug screening and supports further research on these compounds as potential therapies for brain cancer.<\/jats:p>","DOI":"10.3390\/ijms27167139","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T09:24:53Z","timestamp":1786353893000},"page":"7139","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Saudi Patient-Derived Brain and Intracranial Explanted Tumor Cells: Isolation, Growth, and Anticancer Drug Screening"],"prefix":"10.3390","volume":"27","author":[{"given":"Saba M.","family":"Alsubaie","sequence":"first","affiliation":[{"name":"King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 14811, Saudi Arabia"},{"name":"Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafa","family":"Almeer","sequence":"additional","affiliation":[{"name":"Zoology Department, King Saud University, Riyadh 11451, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali H.","family":"Alassiri","sequence":"additional","affiliation":[{"name":"King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 14811, Saudi Arabia"},{"name":"Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City, Ministry of National Guard\u2014Health Affairs, Riyadh 11426, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Alkhani","sequence":"additional","affiliation":[{"name":"Division of Neurosurgery, Department of Surgery, King Abdulaziz Medical City, Ministry of National Guard\u2014Health Affairs, Riyadh 11426, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahd","family":"AlSufiani","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, King Abdulaziz Medical City, Ministry of National Guard\u2014Health Affairs, Riyadh 11426, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imadul","family":"Islam","sequence":"additional","affiliation":[{"name":"King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 14811, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1009-4031","authenticated-orcid":false,"given":"Mohamed","family":"Boudjelal","sequence":"additional","affiliation":[{"name":"King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 14811, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rizwan","family":"Ali","sequence":"additional","affiliation":[{"name":"King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 14811, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"441","DOI":"10.4103\/0019-509X.200658","article-title":"Cancer and cure: A critical analysis","volume":"53","author":"Roy","year":"2016","journal-title":"Indian J. 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